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Protein

Foldase protein PrsA

Gene

prsA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential protein that plays a major role in protein secretion by helping the post-translocational extracellular folding of several secreted proteins. Has PPIase activity but it is not essential for its function in vivo.1 Publication

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywordsi

Molecular functionIsomerase, Rotamase

Enzyme and pathway databases

BioCyciBSUB:BSU09950-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Foldase protein PrsA (EC:5.2.1.8)
Gene namesi
Name:prsA
Ordered Locus Names:BSU09950
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi20C → Y: Abolishes lipid modification of the protein. 1 Publication1
Mutagenesisi141H → A: Loss of PPIase function. No effect on secretion of proteins and on growth. 1 Publication1
Mutagenesisi163F → Y: No effect on secretion of proteins and on growth. 1 Publication1
Mutagenesisi171S → A: No effect on secretion of proteins and on growth. 1 Publication1
Mutagenesisi173D → A: Decrease in PPIase activity. No effect on secretion of proteins and on growth. 1 Publication1
Mutagenesisi175S → P: No effect on secretion of proteins and on growth. 1
Mutagenesisi180G → A: No effect on secretion of proteins and on growth. 1 Publication1
Mutagenesisi191M → A: No effect on secretion of proteins and on growth. 1 Publication1
Mutagenesisi195F → A or Y: No effect on secretion of proteins and on growth. 1 Publication1
Mutagenesisi215Y → A: No effect on secretion of proteins and on growth. 1 Publication1
Mutagenesisi216G → A: Decreased secretion of proteins; no effect on growth. 1 Publication1
Mutagenesisi218H → A: Decreased secretion of proteins; no effect on growth. 1 Publication1
Mutagenesisi219I → A: No effect on secretion of proteins and on growth. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000002929920 – 292Foldase protein PrsAAdd BLAST273

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi20N-palmitoyl cysteineSequence analysis1
Lipidationi20S-diacylglycerol cysteineSequence analysis1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP24327.
PRIDEiP24327.

Interactioni

Protein-protein interaction databases

IntActiP24327. 3 interactors.
MINTiMINT-8365681.
STRINGi224308.Bsubs1_010100005536.

Structurei

Secondary structure

1292
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi23 – 25Combined sources3
Beta strandi27 – 31Combined sources5
Helixi38 – 66Combined sources29
Helixi71 – 84Combined sources14
Helixi88 – 95Combined sources8
Helixi98 – 118Combined sources21
Helixi123 – 130Combined sources8
Beta strandi136 – 145Combined sources10
Helixi147 – 159Combined sources13
Helixi163 – 170Combined sources8
Helixi174 – 176Combined sources3
Turni177 – 180Combined sources4
Beta strandi181 – 191Combined sources11
Helixi193 – 200Combined sources8
Beta strandi217 – 224Combined sources8
Turni229 – 231Combined sources3
Helixi233 – 245Combined sources13
Helixi248 – 261Combined sources14
Helixi269 – 271Combined sources3
Turni272 – 276Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZK6NMR-A135-225[»]
4WO7X-ray2.63A/B21-280[»]
ProteinModelPortaliP24327.
SMRiP24327.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24327.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini134 – 224PpiCAdd BLAST91

Domaini

All three domains (PPIase domain and the flanking N- and C-terminal domains) are essential for activity.

Sequence similaritiesi

Belongs to the PrsA family.Curated
Contains 1 PpiC domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107UUG. Bacteria.
COG0760. LUCA.
HOGENOMiHOG000014031.
InParanoidiP24327.
KOiK07533.
OMAiPGKMVKE.
PhylomeDBiP24327.

Family and domain databases

HAMAPiMF_01145. Foldase_PrsA. 1 hit.
InterProiIPR023059. Foldase_PrsA.
IPR000297. PPIase_PpiC.
IPR023058. PPIase_PpiC_CS.
IPR008880. Trigger_fac_C.
IPR027304. Trigger_fact/SurA_dom.
[Graphical view]
PfamiPF05698. Trigger_C. 1 hit.
[Graphical view]
SUPFAMiSSF109998. SSF109998. 1 hit.
PROSITEiPS01096. PPIC_PPIASE_1. 1 hit.
PS50198. PPIC_PPIASE_2. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24327-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKIAIAAIT ATSILALSAC SSGDKEVIAK TDAGDVTKGE LYTNMKKTAG
60 70 80 90 100
ASVLTQLVQE KVLDKKYKVS DKEIDNKLKE YKTQLGDQYT ALEKQYGKDY
110 120 130 140 150
LKEQVKYELL TQKAAKDNIK VTDADIKEYW EGLKGKIRAS HILVADKKTA
160 170 180 190 200
EEVEKKLKKG EKFEDLAKEY STDSSASKGG DLGWFAKEGQ MDETFSKAAF
210 220 230 240 250
KLKTGEVSDP VKTQYGYHII KKTEERGKYD DMKKELKSEV LEQKLNDNAA
260 270 280 290
VQEAVQKVMK KADIEVKDKD LKDTFNTSST SNSTSSSSSN SK
Length:292
Mass (Da):32,510
Last modified:March 1, 1992 - v1
Checksum:iDEC194D8FE5C9FC0
GO

Sequence cautioni

The sequence AAA22825 differs from that shown. Sequencing errors. The N-terminus of this sequence is probably the N-terminus of phrI.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57271 Genomic DNA. Translation: CAA40543.1.
Y14077 Genomic DNA. Translation: CAA74418.1.
AL009126 Genomic DNA. Translation: CAB12835.1.
M22909 Genomic DNA. Translation: AAA22825.1. Sequence problems.
PIRiS15269.
RefSeqiNP_388876.1. NC_000964.3.
WP_003245079.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB12835; CAB12835; BSU09950.
GeneIDi939294.
KEGGibsu:BSU09950.
PATRICi18973686. VBIBacSub10457_1037.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57271 Genomic DNA. Translation: CAA40543.1.
Y14077 Genomic DNA. Translation: CAA74418.1.
AL009126 Genomic DNA. Translation: CAB12835.1.
M22909 Genomic DNA. Translation: AAA22825.1. Sequence problems.
PIRiS15269.
RefSeqiNP_388876.1. NC_000964.3.
WP_003245079.1. NZ_JNCM01000035.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZK6NMR-A135-225[»]
4WO7X-ray2.63A/B21-280[»]
ProteinModelPortaliP24327.
SMRiP24327.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP24327. 3 interactors.
MINTiMINT-8365681.
STRINGi224308.Bsubs1_010100005536.

Proteomic databases

PaxDbiP24327.
PRIDEiP24327.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12835; CAB12835; BSU09950.
GeneIDi939294.
KEGGibsu:BSU09950.
PATRICi18973686. VBIBacSub10457_1037.

Phylogenomic databases

eggNOGiENOG4107UUG. Bacteria.
COG0760. LUCA.
HOGENOMiHOG000014031.
InParanoidiP24327.
KOiK07533.
OMAiPGKMVKE.
PhylomeDBiP24327.

Enzyme and pathway databases

BioCyciBSUB:BSU09950-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP24327.

Family and domain databases

HAMAPiMF_01145. Foldase_PrsA. 1 hit.
InterProiIPR023059. Foldase_PrsA.
IPR000297. PPIase_PpiC.
IPR023058. PPIase_PpiC_CS.
IPR008880. Trigger_fac_C.
IPR027304. Trigger_fact/SurA_dom.
[Graphical view]
PfamiPF05698. Trigger_C. 1 hit.
[Graphical view]
SUPFAMiSSF109998. SSF109998. 1 hit.
PROSITEiPS01096. PPIC_PPIASE_1. 1 hit.
PS50198. PPIC_PPIASE_2. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRSA_BACSU
AccessioniPrimary (citable) accession number: P24327
Secondary accession number(s): Q45680
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 2, 2016
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.