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Protein

UDP-glucose 4-epimerase

Gene

galE

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in the metabolism of galactose. Catalyzes the conversion of UDP-galactose (UDP-Gal) to UDP-glucose (UDP-Glc) through a mechanism involving the transient reduction of NAD. By controlling the internal galactose concentration, it may be linked to the biosynthesis of lipopolysaccharide surface molecules, which are important for the pathogenesis of H.influenzae.1 Publication

Catalytic activityi

UDP-alpha-D-glucose = UDP-alpha-D-galactose.

Cofactori

NAD(+)By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991NADBy similarity
Binding sitei124 – 1241NADBy similarity
Binding sitei124 – 1241SubstrateBy similarity
Active sitei149 – 1491Proton acceptorBy similarity
Binding sitei149 – 1491NADBy similarity
Binding sitei149 – 1491SubstrateBy similarity
Binding sitei153 – 1531NADBy similarity
Binding sitei178 – 1781NAD; via carbonyl oxygenBy similarity
Binding sitei179 – 1791SubstrateBy similarity
Binding sitei231 – 2311SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 122NADBy similarity
Nucleotide bindingi31 – 366NADBy similarity
Nucleotide bindingi58 – 592NADBy similarity
Nucleotide bindingi80 – 845NADBy similarity

GO - Molecular functioni

  1. coenzyme binding Source: InterPro
  2. UDP-glucose 4-epimerase activity Source: UniProtKB-EC

GO - Biological processi

  1. galactose metabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism, Galactose metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00214.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucose 4-epimerase (EC:5.1.3.2)
Alternative name(s):
Galactowaldenase
UDP-galactose 4-epimerase
Gene namesi
Name:galE
Ordered Locus Names:HI_0351
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338UDP-glucose 4-epimerasePRO_0000183205Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi71421.HI0351.

Structurei

3D structure databases

ProteinModelPortaliP24325.
SMRiP24325. Positions 2-337.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni199 – 2002Substrate bindingBy similarity
Regioni216 – 2183Substrate bindingBy similarity
Regioni292 – 2954Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1087.
KOiK01784.
OMAiPKYNSLE.
OrthoDBiEOG6WHNS9.
PhylomeDBiP24325.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR025308. Epimerase_C.
IPR001509. Epimerase_deHydtase_N.
IPR005886. GalE.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10366:SF39. PTHR10366:SF39. 1 hit.
PfamiPF01370. Epimerase. 1 hit.
PF13950. Epimerase_Csub. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01179. galE. 1 hit.

Sequencei

Sequence statusi: Complete.

P24325-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAILVTGGAG YIGSHTVVEL LNVGKEVVVL DNLCNSSPKS LERVKQITGK
60 70 80 90 100
EAKFYEGDIL DRALLQKIFA ENEINSVIHF AGLKAVGESV QKPTEYYMNN
110 120 130 140 150
VAGTLVLIQE MKKAGVWNFV FSSSATVYGD PKIIPITEDC EVGGTTNPYG
160 170 180 190 200
TSKYMVEQIL RDTAKAEPKF SMTILRYFNP VGAHESGLIG EDPNGIPNNL
210 220 230 240 250
LPYISQVAIG KLAQLSVFGS DYDTHDGTGV RDYIHVVDLA VGHLKALQRH
260 270 280 290 300
ENDAGLHIYN LGTGHGYSVL DMVKAFEKAN NITIAYKLVE RRSGDIATCY
310 320 330
SDPSLAAKEL GWVAERGLEK MMQDTWNWQK NNPKGYRD
Length:338
Mass (Da):37,165
Last modified:November 1, 1995 - v2
Checksum:i449934B0E21C8A56
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti285 – 2851A → P in strain: RM 7004.
Natural varianti333 – 3331P → S in strain: RM 7004.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57315 Genomic DNA. Translation: CAA40568.1.
L42023 Genomic DNA. Translation: AAC22012.1.
PIRiA64063.
RefSeqiNP_438515.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22012; AAC22012; HI_0351.
GeneIDi949642.
KEGGihin:HI0351.
PATRICi20189249. VBIHaeInf48452_0370.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57315 Genomic DNA. Translation: CAA40568.1.
L42023 Genomic DNA. Translation: AAC22012.1.
PIRiA64063.
RefSeqiNP_438515.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP24325.
SMRiP24325. Positions 2-337.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0351.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22012; AAC22012; HI_0351.
GeneIDi949642.
KEGGihin:HI0351.
PATRICi20189249. VBIHaeInf48452_0370.

Phylogenomic databases

eggNOGiCOG1087.
KOiK01784.
OMAiPKYNSLE.
OrthoDBiEOG6WHNS9.
PhylomeDBiP24325.

Enzyme and pathway databases

UniPathwayiUPA00214.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR025308. Epimerase_C.
IPR001509. Epimerase_deHydtase_N.
IPR005886. GalE.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10366:SF39. PTHR10366:SF39. 1 hit.
PfamiPF01370. Epimerase. 1 hit.
PF13950. Epimerase_Csub. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01179. galE. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular analysis of a complex locus from Haemophilus influenzae involved in phase-variable lipopolysaccharide biosynthesis."
    Maskell D.J., Szabo M.J., Butler P.D., Williams A.E., Moxon E.R.
    Mol. Microbiol. 5:1013-1022(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: RM 7004 / Serotype B.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiGALE_HAEIN
AccessioniPrimary (citable) accession number: P24325
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 1, 1995
Last modified: April 1, 2015
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.