P24325 (GALE_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-glucose 4-epimerase
EC=5.1.3.2

Alternative name(s):
Galactowaldenase
UDP-galactose 4-epimerase

Gene names

Name:	galE
Ordered Locus Names:	HI_0351

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	By controlling the internal galactose concentration, it may be linked to the biosynthesis of lipopolysaccharide surface molecules, which are important for the pathogenesis of H.influenzae.
Catalytic activity	UDP-alpha-D-glucose = UDP-alpha-D-galactose.
Cofactor	NAD.
Pathway	Carbohydrate metabolism; galactose metabolism.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the sugar epimerase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Galactose metabolism
Ligand	NAD
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular metabolic process Inferred from electronic annotation. Source: InterPro galactose metabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	UDP-glucose 4-epimerase activity Inferred from electronic annotation. Source: EC coenzyme binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	UDP-glucose 4-epimerase		PRO_0000183205
Regions
	Nucleotide binding	2 – 33	32	NAD By similarity
Sites
	Active site	149	1	Proton acceptor By similarity
	Binding site	124	1	Substrate By similarity
Natural variations
	Natural variant	285	1	A → P in strain: RM 7004.
	Natural variant	333	1	P → S in strain: RM 7004.

Sequences

	Sequence		Length	Mass (Da)
	P24325 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 449934B0E21C8A56	FASTA	338	37,165
	10 20 30 40 50 60 MAILVTGGAG YIGSHTVVEL LNVGKEVVVL DNLCNSSPKS LERVKQITGK EAKFYEGDIL 70 80 90 100 110 120 DRALLQKIFA ENEINSVIHF AGLKAVGESV QKPTEYYMNN VAGTLVLIQE MKKAGVWNFV 130 140 150 160 170 180 FSSSATVYGD PKIIPITEDC EVGGTTNPYG TSKYMVEQIL RDTAKAEPKF SMTILRYFNP 190 200 210 220 230 240 VGAHESGLIG EDPNGIPNNL LPYISQVAIG KLAQLSVFGS DYDTHDGTGV RDYIHVVDLA 250 260 270 280 290 300 VGHLKALQRH ENDAGLHIYN LGTGHGYSVL DMVKAFEKAN NITIAYKLVE RRSGDIATCY 310 320 330 SDPSLAAKEL GWVAERGLEK MMQDTWNWQK NNPKGYRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular analysis of a complex locus from Haemophilus influenzae involved in phase-variable lipopolysaccharide biosynthesis." Maskell D.J., Szabo M.J., Butler P.D., Williams A.E., Moxon E.R. Mol. Microbiol. 5:1013-1022(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RM 7004 / Serotype B.
[2]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X57315 Genomic DNA. Translation: CAA40568.1. L42023 Genomic DNA. Translation: AAC22012.1.
PIR	A64063.
RefSeq	NP_438515.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P24325.
SMR	P24325. Positions 2-337.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI0351.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC22012; AAC22012; HI_0351.
GeneID	949642.
KEGG	hin:HI0351.
PATRIC	20189249. VBIHaeInf48452_0370.
Phylogenomic databases
eggNOG	COG1087.
KO	K01784.
OMA	TENNIDW.
ProtClustDB	CLSK870036.
Enzyme and pathway databases
UniPathway	UPA00214.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR025308. Epimerase_C. IPR001509. Epimerase_deHydtase. IPR005886. GalE. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR10366:SF39. PTHR10366:SF39. 1 hit.
Pfam	PF01370. Epimerase. 1 hit. PF13950. Epimerase_Csub. 1 hit. [Graphical view]
TIGRFAMs	TIGR01179. galE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GALE_HAEIN

Accession

Primary (citable) accession number: P24325

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program