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Protein

UDP-glucose 4-epimerase

Gene

galE

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Involved in the metabolism of galactose. Catalyzes the conversion of UDP-galactose (UDP-Gal) to UDP-glucose (UDP-Glc) through a mechanism involving the transient reduction of NAD. By controlling the internal galactose concentration, it may be linked to the biosynthesis of lipopolysaccharide surface molecules, which are important for the pathogenesis of H.influenzae.1 Publication

Catalytic activityi

UDP-alpha-D-glucose = UDP-alpha-D-galactose.

Cofactori

NAD+By similarity

Pathwayi: galactose metabolism

This protein is involved in the pathway galactose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway galactose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99NADBy similarity1
Binding sitei124NADBy similarity1
Binding sitei124SubstrateBy similarity1
Active sitei149Proton acceptorBy similarity1
Binding sitei149NADBy similarity1
Binding sitei149SubstrateBy similarity1
Binding sitei153NADBy similarity1
Binding sitei178NAD; via carbonyl oxygenBy similarity1
Binding sitei179SubstrateBy similarity1
Binding sitei231SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 12NADBy similarity2
Nucleotide bindingi31 – 36NADBy similarity6
Nucleotide bindingi58 – 59NADBy similarity2
Nucleotide bindingi80 – 84NADBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism, Galactose metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00214.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucose 4-epimerase (EC:5.1.3.2)
Alternative name(s):
Galactowaldenase
UDP-galactose 4-epimerase
Gene namesi
Name:galE
Ordered Locus Names:HI_0351
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001832051 – 338UDP-glucose 4-epimeraseAdd BLAST338

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi71421.HI0351.

Structurei

3D structure databases

ProteinModelPortaliP24325.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni199 – 200Substrate bindingBy similarity2
Regioni216 – 218Substrate bindingBy similarity3
Regioni292 – 295Substrate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CMR. Bacteria.
COG1087. LUCA.
KOiK01784.
OMAiTNLRYFN.
PhylomeDBiP24325.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR001509. Epimerase_deHydtase.
IPR005886. GalE.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01370. Epimerase. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01179. galE. 1 hit.

Sequencei

Sequence statusi: Complete.

P24325-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAILVTGGAG YIGSHTVVEL LNVGKEVVVL DNLCNSSPKS LERVKQITGK
60 70 80 90 100
EAKFYEGDIL DRALLQKIFA ENEINSVIHF AGLKAVGESV QKPTEYYMNN
110 120 130 140 150
VAGTLVLIQE MKKAGVWNFV FSSSATVYGD PKIIPITEDC EVGGTTNPYG
160 170 180 190 200
TSKYMVEQIL RDTAKAEPKF SMTILRYFNP VGAHESGLIG EDPNGIPNNL
210 220 230 240 250
LPYISQVAIG KLAQLSVFGS DYDTHDGTGV RDYIHVVDLA VGHLKALQRH
260 270 280 290 300
ENDAGLHIYN LGTGHGYSVL DMVKAFEKAN NITIAYKLVE RRSGDIATCY
310 320 330
SDPSLAAKEL GWVAERGLEK MMQDTWNWQK NNPKGYRD
Length:338
Mass (Da):37,165
Last modified:November 1, 1995 - v2
Checksum:i449934B0E21C8A56
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti285A → P in strain: RM 7004. 1
Natural varianti333P → S in strain: RM 7004. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57315 Genomic DNA. Translation: CAA40568.1.
L42023 Genomic DNA. Translation: AAC22012.1.
PIRiA64063.
RefSeqiNP_438515.1. NC_000907.1.
WP_005694325.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22012; AAC22012; HI_0351.
GeneIDi949642.
KEGGihin:HI0351.
PATRICi20189249. VBIHaeInf48452_0370.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57315 Genomic DNA. Translation: CAA40568.1.
L42023 Genomic DNA. Translation: AAC22012.1.
PIRiA64063.
RefSeqiNP_438515.1. NC_000907.1.
WP_005694325.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP24325.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0351.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22012; AAC22012; HI_0351.
GeneIDi949642.
KEGGihin:HI0351.
PATRICi20189249. VBIHaeInf48452_0370.

Phylogenomic databases

eggNOGiENOG4105CMR. Bacteria.
COG1087. LUCA.
KOiK01784.
OMAiTNLRYFN.
PhylomeDBiP24325.

Enzyme and pathway databases

UniPathwayiUPA00214.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR001509. Epimerase_deHydtase.
IPR005886. GalE.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01370. Epimerase. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01179. galE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGALE_HAEIN
AccessioniPrimary (citable) accession number: P24325
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.