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P24325

- GALE_HAEIN

UniProt

P24325 - GALE_HAEIN

Protein

UDP-glucose 4-epimerase

Gene

galE

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Involved in the metabolism of galactose. Catalyzes the conversion of UDP-galactose (UDP-Gal) to UDP-glucose (UDP-Glc) through a mechanism involving the transient reduction of NAD. By controlling the internal galactose concentration, it may be linked to the biosynthesis of lipopolysaccharide surface molecules, which are important for the pathogenesis of H.influenzae.1 Publication

    Catalytic activityi

    UDP-alpha-D-glucose = UDP-alpha-D-galactose.

    Cofactori

    NAD.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei99 – 991NADBy similarity
    Binding sitei124 – 1241NADBy similarity
    Binding sitei124 – 1241SubstrateBy similarity
    Active sitei149 – 1491Proton acceptorBy similarity
    Binding sitei149 – 1491NADBy similarity
    Binding sitei149 – 1491SubstrateBy similarity
    Binding sitei153 – 1531NADBy similarity
    Binding sitei178 – 1781NAD; via carbonyl oxygenBy similarity
    Binding sitei179 – 1791SubstrateBy similarity
    Binding sitei231 – 2311SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 122NADBy similarity
    Nucleotide bindingi31 – 366NADBy similarity
    Nucleotide bindingi58 – 592NADBy similarity
    Nucleotide bindingi80 – 845NADBy similarity

    GO - Molecular functioni

    1. coenzyme binding Source: InterPro
    2. UDP-glucose 4-epimerase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular metabolic process Source: InterPro
    2. galactose metabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Carbohydrate metabolism, Galactose metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    UniPathwayiUPA00214.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glucose 4-epimerase (EC:5.1.3.2)
    Alternative name(s):
    Galactowaldenase
    UDP-galactose 4-epimerase
    Gene namesi
    Name:galE
    Ordered Locus Names:HI_0351
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 338338UDP-glucose 4-epimerasePRO_0000183205Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi71421.HI0351.

    Structurei

    3D structure databases

    ProteinModelPortaliP24325.
    SMRiP24325. Positions 2-337.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni199 – 2002Substrate bindingBy similarity
    Regioni216 – 2183Substrate bindingBy similarity
    Regioni292 – 2954Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1087.
    KOiK01784.
    OMAiAGESMIR.
    OrthoDBiEOG6WHNS9.
    PhylomeDBiP24325.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR025308. Epimerase_C.
    IPR001509. Epimerase_deHydtase.
    IPR005886. GalE.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR10366:SF39. PTHR10366:SF39. 1 hit.
    PfamiPF01370. Epimerase. 1 hit.
    PF13950. Epimerase_Csub. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01179. galE. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P24325-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAILVTGGAG YIGSHTVVEL LNVGKEVVVL DNLCNSSPKS LERVKQITGK    50
    EAKFYEGDIL DRALLQKIFA ENEINSVIHF AGLKAVGESV QKPTEYYMNN 100
    VAGTLVLIQE MKKAGVWNFV FSSSATVYGD PKIIPITEDC EVGGTTNPYG 150
    TSKYMVEQIL RDTAKAEPKF SMTILRYFNP VGAHESGLIG EDPNGIPNNL 200
    LPYISQVAIG KLAQLSVFGS DYDTHDGTGV RDYIHVVDLA VGHLKALQRH 250
    ENDAGLHIYN LGTGHGYSVL DMVKAFEKAN NITIAYKLVE RRSGDIATCY 300
    SDPSLAAKEL GWVAERGLEK MMQDTWNWQK NNPKGYRD 338
    Length:338
    Mass (Da):37,165
    Last modified:November 1, 1995 - v2
    Checksum:i449934B0E21C8A56
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti285 – 2851A → P in strain: RM 7004.
    Natural varianti333 – 3331P → S in strain: RM 7004.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57315 Genomic DNA. Translation: CAA40568.1.
    L42023 Genomic DNA. Translation: AAC22012.1.
    PIRiA64063.
    RefSeqiNP_438515.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC22012; AAC22012; HI_0351.
    GeneIDi949642.
    KEGGihin:HI0351.
    PATRICi20189249. VBIHaeInf48452_0370.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57315 Genomic DNA. Translation: CAA40568.1 .
    L42023 Genomic DNA. Translation: AAC22012.1 .
    PIRi A64063.
    RefSeqi NP_438515.1. NC_000907.1.

    3D structure databases

    ProteinModelPortali P24325.
    SMRi P24325. Positions 2-337.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI0351.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC22012 ; AAC22012 ; HI_0351 .
    GeneIDi 949642.
    KEGGi hin:HI0351.
    PATRICi 20189249. VBIHaeInf48452_0370.

    Phylogenomic databases

    eggNOGi COG1087.
    KOi K01784.
    OMAi AGESMIR.
    OrthoDBi EOG6WHNS9.
    PhylomeDBi P24325.

    Enzyme and pathway databases

    UniPathwayi UPA00214 .

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR025308. Epimerase_C.
    IPR001509. Epimerase_deHydtase.
    IPR005886. GalE.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR10366:SF39. PTHR10366:SF39. 1 hit.
    Pfami PF01370. Epimerase. 1 hit.
    PF13950. Epimerase_Csub. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01179. galE. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular analysis of a complex locus from Haemophilus influenzae involved in phase-variable lipopolysaccharide biosynthesis."
      Maskell D.J., Szabo M.J., Butler P.D., Williams A.E., Moxon E.R.
      Mol. Microbiol. 5:1013-1022(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: RM 7004 / Serotype B.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

    Entry informationi

    Entry nameiGALE_HAEIN
    AccessioniPrimary (citable) accession number: P24325
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3