Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P24325 (GALE_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-glucose 4-epimerase

EC=5.1.3.2
Alternative name(s):
Galactowaldenase
UDP-galactose 4-epimerase
Gene names
Name:galE
Ordered Locus Names:HI_0351
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length338 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the metabolism of galactose. Catalyzes the conversion of UDP-galactose (UDP-Gal) to UDP-glucose (UDP-Glc) through a mechanism involving the transient reduction of NAD. By controlling the internal galactose concentration, it may be linked to the biosynthesis of lipopolysaccharide surface molecules, which are important for the pathogenesis of H.influenzae. Ref.1

Catalytic activity

UDP-alpha-D-glucose = UDP-alpha-D-galactose.

Cofactor

NAD By similarity.

Pathway

Carbohydrate metabolism; galactose metabolism.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the NAD(P)-dependent epimerase/dehydratase family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Galactose metabolism
   LigandNAD
   Molecular functionIsomerase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular metabolic process

Inferred from electronic annotation. Source: InterPro

galactose metabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionUDP-glucose 4-epimerase activity

Inferred from electronic annotation. Source: UniProtKB-EC

coenzyme binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 338338UDP-glucose 4-epimerase
PRO_0000183205

Regions

Nucleotide binding11 – 122NAD By similarity
Nucleotide binding31 – 366NAD By similarity
Nucleotide binding58 – 592NAD By similarity
Nucleotide binding80 – 845NAD By similarity
Region199 – 2002Substrate binding By similarity
Region216 – 2183Substrate binding By similarity
Region292 – 2954Substrate binding By similarity

Sites

Active site1491Proton acceptor By similarity
Binding site991NAD By similarity
Binding site1241NAD By similarity
Binding site1241Substrate By similarity
Binding site1491NAD By similarity
Binding site1491Substrate By similarity
Binding site1531NAD By similarity
Binding site1781NAD; via carbonyl oxygen By similarity
Binding site1791Substrate By similarity
Binding site2311Substrate By similarity

Natural variations

Natural variant2851A → P in strain: RM 7004.
Natural variant3331P → S in strain: RM 7004.

Sequences

Sequence LengthMass (Da)Tools
P24325 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 449934B0E21C8A56

FASTA33837,165
        10         20         30         40         50         60 
MAILVTGGAG YIGSHTVVEL LNVGKEVVVL DNLCNSSPKS LERVKQITGK EAKFYEGDIL 

        70         80         90        100        110        120 
DRALLQKIFA ENEINSVIHF AGLKAVGESV QKPTEYYMNN VAGTLVLIQE MKKAGVWNFV 

       130        140        150        160        170        180 
FSSSATVYGD PKIIPITEDC EVGGTTNPYG TSKYMVEQIL RDTAKAEPKF SMTILRYFNP 

       190        200        210        220        230        240 
VGAHESGLIG EDPNGIPNNL LPYISQVAIG KLAQLSVFGS DYDTHDGTGV RDYIHVVDLA 

       250        260        270        280        290        300 
VGHLKALQRH ENDAGLHIYN LGTGHGYSVL DMVKAFEKAN NITIAYKLVE RRSGDIATCY 

       310        320        330 
SDPSLAAKEL GWVAERGLEK MMQDTWNWQK NNPKGYRD 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X57315 Genomic DNA. Translation: CAA40568.1.
L42023 Genomic DNA. Translation: AAC22012.1.
PIRA64063.
RefSeqNP_438515.1. NC_000907.1.

3D structure databases

ProteinModelPortalP24325.
SMRP24325. Positions 2-337.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING71421.HI0351.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC22012; AAC22012; HI_0351.
GeneID949642.
KEGGhin:HI0351.
PATRIC20189249. VBIHaeInf48452_0370.

Phylogenomic databases

eggNOGCOG1087.
KOK01784.
OMAGTFILCE.
OrthoDBEOG6WHNS9.
ProtClustDBCLSK870036.

Enzyme and pathway databases

UniPathwayUPA00214.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR025308. Epimerase_C.
IPR001509. Epimerase_deHydtase.
IPR005886. GalE.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR10366:SF39. PTHR10366:SF39. 1 hit.
PfamPF01370. Epimerase. 1 hit.
PF13950. Epimerase_Csub. 1 hit.
[Graphical view]
TIGRFAMsTIGR01179. galE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGALE_HAEIN
AccessionPrimary (citable) accession number: P24325
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 1, 1995
Last modified: November 13, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names