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P24319 (RS8_THETH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
30S ribosomal protein S8
Gene names
Name:rpsH
Synonyms:rps8
OrganismThermus thermophilus
Taxonomic identifier274 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length138 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit By similarity. HAMAP-Rule MF_01302_B

Subunit structure

Part of the 30S ribosomal subunit. Contacts proteins S5 and S12 By similarity.

Sequence similarities

Belongs to the ribosomal protein S8P family.

Ontologies

Keywords
   LigandRNA-binding
rRNA-binding
   Molecular functionRibonucleoprotein
Ribosomal protein
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processtranslation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentribosome

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionrRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

structural constituent of ribosome

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13813830S ribosomal protein S8 HAMAP-Rule MF_01302_B
PRO_0000126512

Experimental info

Sequence conflict641K → N in CAA62287. Ref.2

Secondary structure

............................... 138
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P24319 [UniParc].

Last modified May 10, 2005. Version 2.
Checksum: 49CC858AD1E4DB5A

FASTA13815,838
        10         20         30         40         50         60 
MLTDPIADML TRIRNATRVY KESTDVPASR FKEEILRILA REGFIKGYER VDVDGKPYLR 

        70         80         90        100        110        120 
VYLKYGPRRQ GPDPRPEQVI HHIRRISKPG RRVYVGVKEI PRVRRGLGIA ILSTSKGVLT 

       130 
DREARKLGVG GELICEVW 

« Hide

References

[1]"The ribosomal protein S8 from Thermus thermophilus VK1. Sequencing of the gene, overexpression of the protein in Escherichia coli and interaction with rRNA."
Vysotskaya V.S., Tischenko S., Garber M.B., Kern D., Ehresmann C., Ehresmann B.
Eur. J. Biochem. 223:437-445(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: VK1.
[2]"Sequencing and analysis of the Thermus thermophilus ribosomal protein gene cluster equivalent to the spectinomycin operon."
Vysotskaya V.S., Shcherbakov D.V., Garber M.B.
Gene 193:23-30(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 38-138.
Strain: VK1.
[3]"Crystal structure of ribosomal protein S8 from Thermus thermophilus reveals a high degree of structural conservation of a specific RNA binding site."
Nevskaya N., Tishchenko S., Nikulin A., Al-Karadaghi S., Liljas A., Ehresmann B., Ehresmann C., Garber M.B., Nikonov S.
J. Mol. Biol. 279:233-244(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
Strain: VK1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X79551 Genomic DNA. Translation: CAA56094.1.
X90765 Genomic DNA. Translation: CAA62287.1.
PIRS15441.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AN7X-ray2.90A/B3-138[»]
1TWTmodel-K1-138[»]
4BYBX-ray3.35H1-138[»]
ProteinModelPortalP24319.
SMRP24319. Positions 1-138.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING262724.TTC1314.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

HAMAPMF_01302_B. Ribosomal_S8_B.
InterProIPR000630. Ribosomal_S8.
[Graphical view]
PANTHERPTHR11758. PTHR11758. 1 hit.
PfamPF00410. Ribosomal_S8. 1 hit.
[Graphical view]
SUPFAMSSF56047. SSF56047. 1 hit.
PROSITEPS00053. RIBOSOMAL_S8. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP24319.

Entry information

Entry nameRS8_THETH
AccessionPrimary (citable) accession number: P24319
Secondary accession number(s): P74907
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: May 10, 2005
Last modified: February 19, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references