P24319 (RS8_THETH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
30S ribosomal protein S8

Gene names

Name:	rpsH
Synonyms:	rps8

Organism

Thermus thermophilus

Taxonomic identifier

274 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	138 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit By similarity. HAMAP-Rule MF_01302_B
Subunit structure	Part of the 30S ribosomal subunit. Contacts proteins S5 and S12 By similarity.
Sequence similarities	Belongs to the ribosomal protein S8P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Cellular_component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 138

138

30S ribosomal protein S8 HAMAP-Rule MF_01302_B

PRO_0000126512

Experimental info

Sequence conflict

64

1

K → N in CAA62287. Ref.2

Secondary structure

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138

Helix Strand Turn

Details...

Helix

5 – 17

13

Turn

18 – 20

3

Beta strand

22 – 27

6

Helix

30 – 41

12

Beta strand

44 – 53

10

Beta strand

56 – 63

8

Beta strand

65 – 68

4

Beta strand

70 – 72

3

Beta strand

75 – 79

5

Beta strand

83 – 85

3

Helix

97 – 99

3

Helix

103 – 106

4

Beta strand

108 – 114

7

Beta strand

117 – 120

4

Helix

121 – 127

7

Beta strand

131 – 137

7

Sequences

	Sequence		Length	Mass (Da)
	P24319 [UniParc]. Last modified May 10, 2005. Version 2. Checksum: 49CC858AD1E4DB5A	FASTA	138	15,838
	10 20 30 40 50 60 MLTDPIADML TRIRNATRVY KESTDVPASR FKEEILRILA REGFIKGYER VDVDGKPYLR 70 80 90 100 110 120 VYLKYGPRRQ GPDPRPEQVI HHIRRISKPG RRVYVGVKEI PRVRRGLGIA ILSTSKGVLT 130 DREARKLGVG GELICEVW

References

[1]	"The ribosomal protein S8 from Thermus thermophilus VK1. Sequencing of the gene, overexpression of the protein in Escherichia coli and interaction with rRNA." Vysotskaya V.S., Tischenko S., Garber M.B., Kern D., Ehresmann C., Ehresmann B. Eur. J. Biochem. 223:437-445(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: VK1.
[2]	"Sequencing and analysis of the Thermus thermophilus ribosomal protein gene cluster equivalent to the spectinomycin operon." Vysotskaya V.S., Shcherbakov D.V., Garber M.B. Gene 193:23-30(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 38-138. Strain: VK1.
[3]	"Crystal structure of ribosomal protein S8 from Thermus thermophilus reveals a high degree of structural conservation of a specific RNA binding site." Nevskaya N., Tishchenko S., Nikulin A., Al-Karadaghi S., Liljas A., Ehresmann B., Ehresmann C., Garber M.B., Nikonov S. J. Mol. Biol. 279:233-244(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS). Strain: VK1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X79551 Genomic DNA. Translation: CAA56094.1.
X90765 Genomic DNA. Translation: CAA62287.1.

PIR

S15441.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AN7	X-ray	2.90	A/B	3-138	[»]
1TWT	model	-	K	1-138	[»]

ProteinModelPortal

P24319.

SMR

P24319. Positions 1-138.

ModBase

Search...

Protein-protein interaction databases

STRING

262724.TTC1314.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

HAMAP

MF_01302_B. Ribosomal_S8_B.

InterPro

IPR000630. Ribosomal_S8.
[Graphical view]

PANTHER

PTHR11758. PTHR11758. 1 hit.

Pfam

PF00410. Ribosomal_S8. 1 hit.
[Graphical view]

SUPFAM

SSF56047. Ribosomal_S8. 1 hit.

PROSITE

PS00053. RIBOSOMAL_S8. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P24319.

Entry information

Entry name

RS8_THETH

Accession

Primary (citable) accession number: P24319
Secondary accession number(s): P74907

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	May 10, 2005
Last modified:	April 3, 2013
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program