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P24319

- RS8_THETH

UniProt

P24319 - RS8_THETH

Protein

30S ribosomal protein S8

Gene

rpsH

Organism
Thermus thermophilus
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
  1. Functioni

    One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.By similarity

    GO - Molecular functioni

    1. rRNA binding Source: UniProtKB-KW
    2. structural constituent of ribosome Source: InterPro

    GO - Biological processi

    1. translation Source: InterPro

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Keywords - Ligandi

    RNA-binding, rRNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    30S ribosomal protein S8
    Gene namesi
    Name:rpsH
    Synonyms:rps8
    OrganismiThermus thermophilus
    Taxonomic identifieri274 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

    Subcellular locationi

    GO - Cellular componenti

    1. ribosome Source: UniProtKB-KW

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13813830S ribosomal protein S8PRO_0000126512Add
    BLAST

    Interactioni

    Subunit structurei

    Part of the 30S ribosomal subunit. Contacts proteins S5 and S12 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi262724.TTC1314.

    Structurei

    Secondary structure

    1
    138
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 1713
    Turni18 – 203
    Beta strandi22 – 276
    Helixi30 – 4112
    Beta strandi44 – 5310
    Beta strandi56 – 638
    Beta strandi65 – 684
    Beta strandi70 – 723
    Beta strandi75 – 795
    Beta strandi83 – 853
    Helixi97 – 993
    Helixi103 – 1064
    Beta strandi108 – 1147
    Beta strandi117 – 1204
    Helixi121 – 1277
    Beta strandi131 – 1377

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AN7X-ray2.90A/B3-138[»]
    1TWTmodel-K1-138[»]
    4BYBX-ray3.35H1-138[»]
    ProteinModelPortaliP24319.
    SMRiP24319. Positions 1-138.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP24319.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein S8P family.Curated

    Family and domain databases

    HAMAPiMF_01302_B. Ribosomal_S8_B.
    InterProiIPR000630. Ribosomal_S8.
    [Graphical view]
    PANTHERiPTHR11758. PTHR11758. 1 hit.
    PfamiPF00410. Ribosomal_S8. 1 hit.
    [Graphical view]
    SUPFAMiSSF56047. SSF56047. 1 hit.
    PROSITEiPS00053. RIBOSOMAL_S8. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24319-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLTDPIADML TRIRNATRVY KESTDVPASR FKEEILRILA REGFIKGYER    50
    VDVDGKPYLR VYLKYGPRRQ GPDPRPEQVI HHIRRISKPG RRVYVGVKEI 100
    PRVRRGLGIA ILSTSKGVLT DREARKLGVG GELICEVW 138
    Length:138
    Mass (Da):15,838
    Last modified:May 10, 2005 - v2
    Checksum:i49CC858AD1E4DB5A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti64 – 641K → N in CAA62287. (PubMed:9249063)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79551 Genomic DNA. Translation: CAA56094.1.
    X90765 Genomic DNA. Translation: CAA62287.1.
    PIRiS15441.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X79551 Genomic DNA. Translation: CAA56094.1 .
    X90765 Genomic DNA. Translation: CAA62287.1 .
    PIRi S15441.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AN7 X-ray 2.90 A/B 3-138 [» ]
    1TWT model - K 1-138 [» ]
    4BYB X-ray 3.35 H 1-138 [» ]
    ProteinModelPortali P24319.
    SMRi P24319. Positions 1-138.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 262724.TTC1314.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P24319.

    Family and domain databases

    HAMAPi MF_01302_B. Ribosomal_S8_B.
    InterProi IPR000630. Ribosomal_S8.
    [Graphical view ]
    PANTHERi PTHR11758. PTHR11758. 1 hit.
    Pfami PF00410. Ribosomal_S8. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56047. SSF56047. 1 hit.
    PROSITEi PS00053. RIBOSOMAL_S8. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ribosomal protein S8 from Thermus thermophilus VK1. Sequencing of the gene, overexpression of the protein in Escherichia coli and interaction with rRNA."
      Vysotskaya V.S., Tischenko S., Garber M.B., Kern D., Ehresmann C., Ehresmann B.
      Eur. J. Biochem. 223:437-445(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: VK1.
    2. "Sequencing and analysis of the Thermus thermophilus ribosomal protein gene cluster equivalent to the spectinomycin operon."
      Vysotskaya V.S., Shcherbakov D.V., Garber M.B.
      Gene 193:23-30(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 38-138.
      Strain: VK1.
    3. "Crystal structure of ribosomal protein S8 from Thermus thermophilus reveals a high degree of structural conservation of a specific RNA binding site."
      Nevskaya N., Tishchenko S., Nikulin A., Al-Karadaghi S., Liljas A., Ehresmann B., Ehresmann C., Garber M.B., Nikonov S.
      J. Mol. Biol. 279:233-244(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).
      Strain: VK1.

    Entry informationi

    Entry nameiRS8_THETH
    AccessioniPrimary (citable) accession number: P24319
    Secondary accession number(s): P74907
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: May 10, 2005
    Last modified: October 1, 2014
    This is version 82 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Ribosomal proteins
      Ribosomal proteins families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3