P24309 (DBR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lariat debranching enzyme
EC=3.1.-.-

Gene names

Name:	DBR1
Synonyms:	PRP26
Ordered Locus Names:	YKL149C
ORF Names:	YKL604

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cleaves the 2'-5' phosphodiester linkage at the branch point of lariat intron pre-mRNAs after splicing and converts them into linear molecules that are subsequently degraded. It thereby facilitates ribonucleotide turnover. It also participates in Ty1 retrovirus-like transposition via a RNA lariat intermediate in cDNA synthesis. Ref.8
Catalytic activity	RNA processing activity that hydrolyzes the 2'-5' phosphodiester linkage at the branchpoint of excised intron lariats.
Subcellular location	Nucleus. Cytoplasm Ref.6.
Miscellaneous	Present with 1130 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the lariat debranching enzyme family.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Cytoplasm Nucleus
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	RNA catabolic process Inferred from mutant phenotype Ref.1. Source: SGD mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW pseudohyphal growth Inferred from mutant phenotype. Source: SGD snoRNA metabolic process Inferred from mutant phenotype. Source: SGD transposition, RNA-mediated Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred by curator Ref.1. Source: SGD
Molecular function	RNA lariat debranching enzyme activity Inferred from mutant phenotype Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 405	405	Lariat debranching enzyme		PRO_0000079797
Amino acid modifications
	Modified residue	269	1	Phosphoserine Ref.9
	Modified residue	270	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P24309 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 437D5325D1D58257	FASTA	405	47,741
	10 20 30 40 50 60 MTKLRIAVQG CCHGQLNQIY KEVSRIHAKT PIDLLIILGD FQSIRDGQDF KSIAIPPKYQ 70 80 90 100 110 120 RLGDFISYYN NEIEAPVPTI FIGGNHESMR HLMLLPHGGY VAKNIFYMGY SNVIWFKGIR 130 140 150 160 170 180 IGSLSGIWKE WDFNKQRPDW NDLENNNWKA NIRNLYHVRI SDIAPLFMIK HRIDIMLSHD 190 200 210 220 230 240 WPNGVVYHGD TKHLLKLKPF FEQDIKEGKL GSPVTWQLLR DLRPQWWLSA HLHVRFMASI 250 260 270 280 290 300 KHNKRSHEPP NKSTSKTKKN NNEIDLDLSS DEDERSGIMN CQEENEYDSK YGETRFLALD 310 320 330 340 350 360 KCLPRRRWLE ILEIEPDTSH ASWKDENHRM FWDPEFINNL VICQKNKNLL SNKPFNSVNW 370 380 390 400 IELSQSNREE GRDIDWENYA IPAYTLDIQK DEVRQTKAFI SKFMT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of the gene encoding yeast debranching enzyme." Chapman K.B., Boeke J.D. Cell 65:483-492(1991) [PubMed: 1850323] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"DNA sequencing of a 36.2 kb fragment located between the FAS1 and LAP loci of chromosome XI of Saccharomyces cerevisiae." Vandenbol M., Bolle P.-A., Dion C., Portetelle D., Hilger F. Yeast 10:S35-S40(1994) [PubMed: 8091859] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed: 8196765] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Primary structure, import, and assembly of the yeast homolog of succinate dehydrogenase flavoprotein." Schuelke N., Blobel G., Pain D. Proc. Natl. Acad. Sci. U.S.A. 89:8011-8015(1992) [PubMed: 1518827] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-117.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"RNA branching and debranching in the yeast retrovirus-like element Ty1." Cheng Z., Menees T.M. Science 303:240-243(2004) [PubMed: 14716018] [Abstract] Cited for: FUNCTION.
[9]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-269 AND SER-270, MASS SPECTROMETRY. Strain: ADR376.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M62813 Genomic DNA. Translation: AAA34560.1. M94874 Genomic DNA. Translation: AAA35025.1. M86909 Genomic DNA. Translation: AAA35023.1. Z26877 Genomic DNA. Translation: CAA81504.1. Z28149 Genomic DNA. Translation: CAA81990.1. BK006944 Genomic DNA. Translation: DAA09014.1.
PIR	A38491.
RefSeq	NP_012773.1. NM_001179715.1.
3D structure databases
ProteinModelPortal	P24309.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4858N.
IntAct	P24309. 1 interaction.
MINT	MINT-499332.
STRING	P24309.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL149C; YKL149C; YKL149C.
GeneID	853708.
KEGG	sce:YKL149C.
NMPDR	fig|4932.3.peg.3752.
Organism-specific databases
CYGD	YKL149c.
SGD	S000001632. DBR1.
Phylogenomic databases
eggNOG	fuNOG05852.
GeneTree	EFGT00050000000586.
HOGENOM	HBG202411.
OMA	RSIAINK.
OrthoDB	EOG4GMZ60.
Gene expression databases
ArrayExpress	P24309.
Genevestigator	P24309.
GermOnline	YKL149C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007708. DBR1_C. IPR004843. Metallo_PEstase_dom. [Graphical view]
KO	K01175.
Pfam	PF05011. DBR1. 1 hit. PF00149. Metallophos. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974710.

Entry information

Entry name

DBR1_YEAST

Accession

Primary (citable) accession number: P24309
Secondary accession number(s): D6VX48

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	December 14, 2011
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program