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P24298

- ALAT1_HUMAN

UniProt

P24298 - ALAT1_HUMAN

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Protein

Alanine aminotransferase 1

Gene

GPT

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles (By similarity).By similarity

Catalytic activityi

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactori

Pathwayi

GO - Molecular functioni

  1. L-alanine:2-oxoglutarate aminotransferase activity Source: UniProtKB
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. cellular amino acid biosynthetic process Source: Reactome
  2. cellular nitrogen compound metabolic process Source: Reactome
  3. L-alanine catabolic process Source: UniProtKB-UniPathway
  4. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:HS09610-MONOMER.
ReactomeiREACT_238. Amino acid synthesis and interconversion (transamination).
UniPathwayiUPA00528; UER00586.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine aminotransferase 1 (EC:2.6.1.2)
Short name:
ALT1
Alternative name(s):
Glutamate pyruvate transaminase 1
Short name:
GPT 1
Glutamic--alanine transaminase 1
Glutamic--pyruvic transaminase 1
Gene namesi
Name:GPT
Synonyms:AAT1, GPT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 8

Organism-specific databases

HGNCiHGNC:4552. GPT.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. extracellular vesicular exosome Source: UniProt
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA28947.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 496495Alanine aminotransferase 1PRO_0000123933Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei314 – 3141N6-(pyridoxal phosphate)lysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP24298.
PaxDbiP24298.
PRIDEiP24298.

PTM databases

PhosphoSiteiP24298.

Expressioni

Tissue specificityi

Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.1 Publication

Inductioni

By glucocorticoids.

Gene expression databases

BgeeiP24298.
CleanExiHS_GPT.
GenevestigatoriP24298.

Organism-specific databases

HPAiCAB032997.
CAB032999.
HPA031059.
HPA031060.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi109133. 2 interactions.
IntActiP24298. 1 interaction.
STRINGi9606.ENSP00000378408.

Structurei

3D structure databases

ProteinModelPortaliP24298.
SMRiP24298. Positions 21-495.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0436.
GeneTreeiENSGT00650000093331.
HOGENOMiHOG000215020.
HOVERGENiHBG026148.
InParanoidiP24298.
KOiK00814.
OMAiHAKFTLE.
OrthoDBiEOG76HQ18.
PhylomeDBiP24298.
TreeFamiTF300839.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24298-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ
60 70 80 90 100
ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF
110 120 130 140 150
PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD
160 170 180 190 200
PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG
210 220 230 240 250
AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ
260 270 280 290 300
TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY
310 320 330 340 350
AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP
360 370 380 390 400
PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA
410 420 430 440 450
PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC
460 470 480 490
VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS
Length:496
Mass (Da):54,637
Last modified:January 23, 2007 - v3
Checksum:i23705E7A3A4283B6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 74STGD → RRGN in BAA01186. 1 PublicationCurated
Sequence conflicti39 – 391G → S in BAA01186. 1 PublicationCurated
Sequence conflicti222 – 2221H → A AA sequence (PubMed:1931970)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti14 – 141H → N in allele GPT*2. 2 Publications
Corresponds to variant rs1063739 [ dbSNP | Ensembl ].
VAR_000561

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70732 Genomic DNA. Translation: AAC51155.1.
BT006992 mRNA. Translation: AAP35638.1.
EF444981 Genomic DNA. Translation: ACA05996.1.
CH471162 Genomic DNA. Translation: EAW82077.1.
CH471162 Genomic DNA. Translation: EAW82078.1.
BC018207 mRNA. Translation: AAH18207.1.
D10355 mRNA. Translation: BAA01186.1.
CCDSiCCDS6430.1.
PIRiA40465.
RefSeqiNP_005300.1. NM_005309.2.
UniGeneiHs.103502.

Genome annotation databases

EnsembliENST00000394955; ENSP00000378408; ENSG00000167701.
ENST00000528431; ENSP00000433586; ENSG00000167701.
GeneIDi2875.
KEGGihsa:2875.
UCSCiuc003zdh.4. human.

Polymorphism databases

DMDMi46577683.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

SHMPD

The Singapore human mutation and polymorphism database

Wikipedia

Alanine transaminase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70732 Genomic DNA. Translation: AAC51155.1 .
BT006992 mRNA. Translation: AAP35638.1 .
EF444981 Genomic DNA. Translation: ACA05996.1 .
CH471162 Genomic DNA. Translation: EAW82077.1 .
CH471162 Genomic DNA. Translation: EAW82078.1 .
BC018207 mRNA. Translation: AAH18207.1 .
D10355 mRNA. Translation: BAA01186.1 .
CCDSi CCDS6430.1.
PIRi A40465.
RefSeqi NP_005300.1. NM_005309.2.
UniGenei Hs.103502.

3D structure databases

ProteinModelPortali P24298.
SMRi P24298. Positions 21-495.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 109133. 2 interactions.
IntActi P24298. 1 interaction.
STRINGi 9606.ENSP00000378408.

Chemistry

ChEMBLi CHEMBL5929.
DrugBanki DB00160. L-Alanine.
DB00780. Phenelzine.

PTM databases

PhosphoSitei P24298.

Polymorphism databases

DMDMi 46577683.

Proteomic databases

MaxQBi P24298.
PaxDbi P24298.
PRIDEi P24298.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000394955 ; ENSP00000378408 ; ENSG00000167701 .
ENST00000528431 ; ENSP00000433586 ; ENSG00000167701 .
GeneIDi 2875.
KEGGi hsa:2875.
UCSCi uc003zdh.4. human.

Organism-specific databases

CTDi 2875.
GeneCardsi GC08P145728.
HGNCi HGNC:4552. GPT.
HPAi CAB032997.
CAB032999.
HPA031059.
HPA031060.
MIMi 138200. gene.
neXtProti NX_P24298.
PharmGKBi PA28947.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0436.
GeneTreei ENSGT00650000093331.
HOGENOMi HOG000215020.
HOVERGENi HBG026148.
InParanoidi P24298.
KOi K00814.
OMAi HAKFTLE.
OrthoDBi EOG76HQ18.
PhylomeDBi P24298.
TreeFami TF300839.

Enzyme and pathway databases

UniPathwayi UPA00528 ; UER00586 .
BioCyci MetaCyc:HS09610-MONOMER.
Reactomei REACT_238. Amino acid synthesis and interconversion (transamination).

Miscellaneous databases

GenomeRNAii 2875.
NextBioi 11349.
PROi P24298.
SOURCEi Search...

Gene expression databases

Bgeei P24298.
CleanExi HS_GPT.
Genevestigatori P24298.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
Pfami PF00155. Aminotran_1_2. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Human glutamate pyruvate transaminase (GPT): localization to 8q24.3, cDNA and genomic sequences, and polymorphic sites."
    Sohocki M.M., Sullivan L.S., Harrison W.R., Sodergren E.J., Elder F.F.B., Weinstock G., Tanase S., Daiger S.P.
    Genomics 40:247-252(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ASN-14.
  2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  3. NHLBI resequencing and genotyping service (RS&G)
    Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ASN-14.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  6. "Complete amino acid sequence of human liver cytosolic alanine aminotransferase (GPT) determined by a combination of conventional and mass spectral methods."
    Ishiguro M., Takio K., Suzuki M., Oyama R., Matsuzawa T., Titani K.
    Biochemistry 30:10451-10457(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-496, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2.
    Tissue: Liver.
  7. "Human mRNA for alanine aminotransferase."
    Funatsu M., Tanase S., Nakao J., Hamada F., Oka T., Morino Y.
    Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-496.
    Tissue: Liver.
  8. "cDNA cloning, genomic structure, chromosomal mapping, and functional expression of a novel human alanine aminotransferase."
    Yang R.-Z., Blaileanu G., Hansen B.C., Shuldiner A.R., Gong D.-W.
    Genomics 79:445-450(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiALAT1_HUMAN
AccessioniPrimary (citable) accession number: P24298
Secondary accession number(s): B0YJ18
, D3DWM7, P78398, Q93076
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 155 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3