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P24298

- ALAT1_HUMAN

UniProt

P24298 - ALAT1_HUMAN

Protein

Alanine aminotransferase 1

Gene

GPT

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 153 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles By similarity.By similarity

    Catalytic activityi

    L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

    Cofactori

    Pyridoxal phosphate.

    Pathwayi

    GO - Molecular functioni

    1. L-alanine:2-oxoglutarate aminotransferase activity Source: UniProtKB
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. cellular amino acid biosynthetic process Source: Reactome
    2. cellular nitrogen compound metabolic process Source: Reactome
    3. L-alanine catabolic process Source: UniProtKB-UniPathway
    4. small molecule metabolic process Source: Reactome

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:HS09610-MONOMER.
    ReactomeiREACT_238. Amino acid synthesis and interconversion (transamination).
    UniPathwayiUPA00528; UER00586.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine aminotransferase 1 (EC:2.6.1.2)
    Short name:
    ALT1
    Alternative name(s):
    Glutamate pyruvate transaminase 1
    Short name:
    GPT 1
    Glutamic--alanine transaminase 1
    Glutamic--pyruvic transaminase 1
    Gene namesi
    Name:GPT
    Synonyms:AAT1, GPT1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 8

    Organism-specific databases

    HGNCiHGNC:4552. GPT.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Reactome
    2. extracellular vesicular exosome Source: UniProt

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA28947.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 496495Alanine aminotransferase 1PRO_0000123933Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei314 – 3141N6-(pyridoxal phosphate)lysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP24298.
    PaxDbiP24298.
    PRIDEiP24298.

    PTM databases

    PhosphoSiteiP24298.

    Expressioni

    Tissue specificityi

    Liver, kidney, heart, and skeletal muscles. Expressed at moderate levels in the adipose tissue.1 Publication

    Inductioni

    By glucocorticoids.

    Gene expression databases

    BgeeiP24298.
    CleanExiHS_GPT.
    GenevestigatoriP24298.

    Organism-specific databases

    HPAiCAB032997.
    CAB032999.
    HPA031059.
    HPA031060.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    BioGridi109133. 2 interactions.
    IntActiP24298. 1 interaction.
    STRINGi9606.ENSP00000378408.

    Structurei

    3D structure databases

    ProteinModelPortaliP24298.
    SMRiP24298. Positions 21-495.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0436.
    HOGENOMiHOG000215020.
    HOVERGENiHBG026148.
    InParanoidiP24298.
    KOiK00814.
    OMAiHAKFTLE.
    OrthoDBiEOG76HQ18.
    PhylomeDBiP24298.
    TreeFamiTF300839.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P24298-1 [UniParc]FASTAAdd to Basket

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    MASSTGDRSQ AVRHGLRAKV LTLDGMNPRV RRVEYAVRGP IVQRALELEQ    50
    ELRQGVKKPF TEVIRANIGD AQAMGQRPIT FLRQVLALCV NPDLLSSPNF 100
    PDDAKKRAER ILQACGGHSL GAYSVSSGIQ LIREDVARYI ERRDGGIPAD 150
    PNNVFLSTGA SDAIVTVLKL LVAGEGHTRT GVLIPIPQYP LYSATLAELG 200
    AVQVDYYLDE ERAWALDVAE LHRALGQARD HCRPRALCVI NPGNPTGQVQ 250
    TRECIEAVIR FAFEERLFLL ADEVYQDNVY AAGSQFHSFK KVLMEMGPPY 300
    AGQQELASFH STSKGYMGEC GFRGGYVEVV NMDAAVQQQM LKLMSVRLCP 350
    PVPGQALLDL VVSPPAPTDP SFAQFQAEKQ AVLAELAAKA KLTEQVFNEA 400
    PGISCNPVQG AMYSFPRVQL PPRAVERAQE LGLAPDMFFC LRLLEETGIC 450
    VVPGSGFGQR EGTYHFRMTI LPPLEKLRLL LEKLSRFHAK FTLEYS 496
    Length:496
    Mass (Da):54,637
    Last modified:January 23, 2007 - v3
    Checksum:i23705E7A3A4283B6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti4 – 74STGD → RRGN in BAA01186. 1 PublicationCurated
    Sequence conflicti39 – 391G → S in BAA01186. 1 PublicationCurated
    Sequence conflicti222 – 2221H → A AA sequence (PubMed:1931970)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti14 – 141H → N in allele GPT*2. 2 Publications
    Corresponds to variant rs1063739 [ dbSNP | Ensembl ].
    VAR_000561

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U70732 Genomic DNA. Translation: AAC51155.1.
    BT006992 mRNA. Translation: AAP35638.1.
    EF444981 Genomic DNA. Translation: ACA05996.1.
    CH471162 Genomic DNA. Translation: EAW82077.1.
    CH471162 Genomic DNA. Translation: EAW82078.1.
    BC018207 mRNA. Translation: AAH18207.1.
    D10355 mRNA. Translation: BAA01186.1.
    CCDSiCCDS6430.1.
    PIRiA40465.
    RefSeqiNP_005300.1. NM_005309.2.
    UniGeneiHs.103502.

    Genome annotation databases

    EnsembliENST00000394955; ENSP00000378408; ENSG00000167701.
    ENST00000528431; ENSP00000433586; ENSG00000167701.
    GeneIDi2875.
    KEGGihsa:2875.
    UCSCiuc003zdh.4. human.

    Polymorphism databases

    DMDMi46577683.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    SHMPD

    The Singapore human mutation and polymorphism database

    Wikipedia

    Alanine transaminase entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U70732 Genomic DNA. Translation: AAC51155.1 .
    BT006992 mRNA. Translation: AAP35638.1 .
    EF444981 Genomic DNA. Translation: ACA05996.1 .
    CH471162 Genomic DNA. Translation: EAW82077.1 .
    CH471162 Genomic DNA. Translation: EAW82078.1 .
    BC018207 mRNA. Translation: AAH18207.1 .
    D10355 mRNA. Translation: BAA01186.1 .
    CCDSi CCDS6430.1.
    PIRi A40465.
    RefSeqi NP_005300.1. NM_005309.2.
    UniGenei Hs.103502.

    3D structure databases

    ProteinModelPortali P24298.
    SMRi P24298. Positions 21-495.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109133. 2 interactions.
    IntActi P24298. 1 interaction.
    STRINGi 9606.ENSP00000378408.

    Chemistry

    ChEMBLi CHEMBL5929.
    DrugBanki DB00160. L-Alanine.
    DB00780. Phenelzine.

    PTM databases

    PhosphoSitei P24298.

    Polymorphism databases

    DMDMi 46577683.

    Proteomic databases

    MaxQBi P24298.
    PaxDbi P24298.
    PRIDEi P24298.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000394955 ; ENSP00000378408 ; ENSG00000167701 .
    ENST00000528431 ; ENSP00000433586 ; ENSG00000167701 .
    GeneIDi 2875.
    KEGGi hsa:2875.
    UCSCi uc003zdh.4. human.

    Organism-specific databases

    CTDi 2875.
    GeneCardsi GC08P145728.
    HGNCi HGNC:4552. GPT.
    HPAi CAB032997.
    CAB032999.
    HPA031059.
    HPA031060.
    MIMi 138200. gene.
    neXtProti NX_P24298.
    PharmGKBi PA28947.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG0436.
    HOGENOMi HOG000215020.
    HOVERGENi HBG026148.
    InParanoidi P24298.
    KOi K00814.
    OMAi HAKFTLE.
    OrthoDBi EOG76HQ18.
    PhylomeDBi P24298.
    TreeFami TF300839.

    Enzyme and pathway databases

    UniPathwayi UPA00528 ; UER00586 .
    BioCyci MetaCyc:HS09610-MONOMER.
    Reactomei REACT_238. Amino acid synthesis and interconversion (transamination).

    Miscellaneous databases

    GenomeRNAii 2875.
    NextBioi 11349.
    PROi P24298.
    SOURCEi Search...

    Gene expression databases

    Bgeei P24298.
    CleanExi HS_GPT.
    Genevestigatori P24298.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Human glutamate pyruvate transaminase (GPT): localization to 8q24.3, cDNA and genomic sequences, and polymorphic sites."
      Sohocki M.M., Sullivan L.S., Harrison W.R., Sodergren E.J., Elder F.F.B., Weinstock G., Tanase S., Daiger S.P.
      Genomics 40:247-252(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ASN-14.
    2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. NHLBI resequencing and genotyping service (RS&G)
      Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT ASN-14.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    6. "Complete amino acid sequence of human liver cytosolic alanine aminotransferase (GPT) determined by a combination of conventional and mass spectral methods."
      Ishiguro M., Takio K., Suzuki M., Oyama R., Matsuzawa T., Titani K.
      Biochemistry 30:10451-10457(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-496, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2.
      Tissue: Liver.
    7. "Human mRNA for alanine aminotransferase."
      Funatsu M., Tanase S., Nakao J., Hamada F., Oka T., Morino Y.
      Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 4-496.
      Tissue: Liver.
    8. "cDNA cloning, genomic structure, chromosomal mapping, and functional expression of a novel human alanine aminotransferase."
      Yang R.-Z., Blaileanu G., Hansen B.C., Shuldiner A.R., Gong D.-W.
      Genomics 79:445-450(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiALAT1_HUMAN
    AccessioniPrimary (citable) accession number: P24298
    Secondary accession number(s): B0YJ18
    , D3DWM7, P78398, Q93076
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 153 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 8
      Human chromosome 8: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3