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Protein

Nuclease P1

Gene
N/A
Organism
Penicillium citrinum
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes only single-stranded DNA and RNA without apparent specificity for bases.

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products.

Cofactori

Zn2+Note: Binds 3 Zn2+ ions.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1Zinc 31
Metal bindingi6Zinc 31
Metal bindingi45Zinc 11
Metal bindingi60Zinc 11
Metal bindingi116Zinc 11
Metal bindingi120Zinc 11
Metal bindingi120Zinc 31
Metal bindingi126Zinc 21
Metal bindingi149Zinc 21
Metal bindingi153Zinc 21

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclease P1 (EC:3.1.30.1)
Alternative name(s):
Deoxyribonuclease P1
Endonuclease P1
OrganismiPenicillium citrinum
Taxonomic identifieri5077 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaePenicillium

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000580031 – 270Nuclease P1Add BLAST270

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi72 ↔ 217
Disulfide bondi80 ↔ 85
Glycosylationi92N-linked (GlcNAc...)1
Glycosylationi138N-linked (GlcNAc...)1
Glycosylationi184N-linked (GlcNAc...)1
Glycosylationi197N-linked (GlcNAc...)1

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

Secondary structure

1270
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 16Combined sources14
Helixi19 – 29Combined sources11
Turni34 – 37Combined sources4
Helixi38 – 40Combined sources3
Helixi43 – 48Combined sources6
Turni51 – 53Combined sources3
Helixi54 – 60Combined sources7
Turni68 – 70Combined sources3
Helixi76 – 79Combined sources4
Helixi86 – 97Combined sources12
Helixi104 – 120Combined sources17
Helixi124 – 127Combined sources4
Helixi130 – 133Combined sources4
Turni134 – 136Combined sources3
Beta strandi138 – 141Combined sources4
Beta strandi144 – 147Combined sources4
Helixi148 – 153Combined sources6
Helixi155 – 161Combined sources7
Helixi166 – 181Combined sources16
Helixi186 – 193Combined sources8
Helixi201 – 218Combined sources18
Helixi226 – 228Combined sources3
Beta strandi229 – 232Combined sources4
Helixi236 – 263Combined sources28

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AK0X-ray1.80A1-270[»]
ProteinModelPortaliP24289.
SMRiP24289.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24289.

Family & Domainsi

Sequence similaritiesi

Belongs to the nuclease type I family.Curated

Family and domain databases

CDDicd11010. S1-P1_nuclease. 1 hit.
Gene3Di1.10.575.10. 1 hit.
InterProiIPR008947. PLipase_C/P1_nuclease.
IPR003154. S1/P1nuclease.
[Graphical view]
PfamiPF02265. S1-P1_nuclease. 1 hit.
[Graphical view]
SUPFAMiSSF48537. SSF48537. 1 hit.

Sequencei

Sequence statusi: Complete.

P24289-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
WGALGHATVA YVAQHYVSPE AASWAQGILG SSSSSYLASI ASWADEYRLT
60 70 80 90 100
SAGKWSASLH FIDAEDNPPT NCNVDYERDC GSSGCSISAI ANYTQRVSDS
110 120 130 140 150
SLSSENHAEA LRFLVHFIGD MTQPLHDEAY AVGGNKINVT FDGYHDNLHS
160 170 180 190 200
DWDTYMPQKL IGGHALSDAE SWAKTLVQNI ESGNYTAQAI GWIKGDNISE
210 220 230 240 250
PITTATRWAS DANALVCTVV MPHGAAALQT GDLYPTYYDS VIDTIELQIA
260 270
KGGYRLANWI NEIHGSEIAK
Length:270
Mass (Da):29,227
Last modified:March 1, 1992 - v1
Checksum:iFA9D25FE75A526EF
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti270Missing .1

Sequence databases

PIRiS17828.

Cross-referencesi

Sequence databases

PIRiS17828.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AK0X-ray1.80A1-270[»]
ProteinModelPortaliP24289.
SMRiP24289.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP24289.

Family and domain databases

CDDicd11010. S1-P1_nuclease. 1 hit.
Gene3Di1.10.575.10. 1 hit.
InterProiIPR008947. PLipase_C/P1_nuclease.
IPR003154. S1/P1nuclease.
[Graphical view]
PfamiPF02265. S1-P1_nuclease. 1 hit.
[Graphical view]
SUPFAMiSSF48537. SSF48537. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNUP1_PENCI
AccessioniPrimary (citable) accession number: P24289
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.