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Protein

Branched-chain-amino-acid aminotransferase, cytosolic

Gene

Bcat1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.

Catalytic activityi

L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate.
L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate.
L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate.

Cofactori

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-MMU-70895. Branched-chain amino acid catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Branched-chain-amino-acid aminotransferase, cytosolic (EC:2.6.1.42)
Short name:
BCAT(c)
Alternative name(s):
Protein ECA39
Gene namesi
Name:Bcat1
Synonyms:Eca39
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:104861. Bcat1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 386386Branched-chain-amino-acid aminotransferase, cytosolicPRO_0000103293Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei222 – 2221N6-(pyridoxal phosphate)lysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP24288.
PaxDbiP24288.
PRIDEiP24288.

PTM databases

iPTMnetiP24288.
PhosphoSiteiP24288.

Expressioni

Tissue specificityi

Expressed in brain and kidney. Overexpressed in MYC-induced brain tumors, lymphomas, as well as in a teratocarcinoma cell line.

Developmental stagei

Highly expressed at day 9 of embryogenesis. Expression decreases to moderate levels through day 13. In the developing embryo, expressed in the brain, somites and mesenophric tubules.

Gene expression databases

BgeeiP24288.
CleanExiMM_BCAT1.
ExpressionAtlasiP24288. baseline and differential.
GenevisibleiP24288. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP24288. 1 interaction.
MINTiMINT-4089144.
STRINGi10090.ENSMUSP00000032402.

Structurei

3D structure databases

ProteinModelPortaliP24288.
SMRiP24288. Positions 24-385.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0975. Eukaryota.
COG0115. LUCA.
HOGENOMiHOG000276704.
HOVERGENiHBG050678.
InParanoidiP24288.
KOiK00826.

Family and domain databases

InterProiIPR001544. Aminotrans_IV.
IPR018300. Aminotrans_IV_CS.
IPR005786. B_amino_transII.
[Graphical view]
PANTHERiPTHR11825. PTHR11825. 1 hit.
PfamiPF01063. Aminotran_4. 1 hit.
[Graphical view]
PIRSFiPIRSF006468. BCAT1. 1 hit.
SUPFAMiSSF56752. SSF56752. 1 hit.
TIGRFAMsiTIGR01123. ilvE_II. 1 hit.
PROSITEiPS00770. AA_TRANSFER_CLASS_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24288-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKDCSNGCSA PFAGERGSEE VAETFRAKDL IITPATVLKE KPDPDSLVFG
60 70 80 90 100
ATFTDHMLTV EWSSASGWEK PHIKPFGNLP IHPAASVLHY AVELFEGLKA
110 120 130 140 150
FRGVDNKIRL FRPDLNMDRM CRSAVRTTLP MFDKEELLKC ILQLLQIDQE
160 170 180 190 200
WVPYSTSASL YIRPTFIGTE PSLGVKKPSK ALLFVILSPV GPYFSSGSFT
210 220 230 240 250
PVSLWANPKY IRAWKGGTGD CKMGGNYGAS LLAQCEAVEN GCQQVLWLYG
260 270 280 290 300
KDNQITEVGT MNLFLYWINE DGEEELATPP LDGIILPGVT RQSILELAQQ
310 320 330 340 350
WGEFKVCERH LTMDDLATAL EGNRVKEMFG SGTACVVCPV SDILYKGQML
360 370 380
HIPTMENGPK LASRILGKLT DIQYGRVESD WTIELP
Length:386
Mass (Da):42,791
Last modified:October 1, 1996 - v2
Checksum:i8185C4C227843B5E
GO

Sequence cautioni

The sequence CAA35543.1 differs from that shown. Reason: Frameshift at positions 277 and 360. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti238 – 2381V → A in AAH53706 (PubMed:15489334).Curated
Sequence conflicti265 – 2651L → P in AAH53706 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U42443 mRNA. Translation: AAB05673.1.
X17502 mRNA. Translation: CAA35543.1. Frameshift.
BC053706 mRNA. Translation: AAH53706.1.
CCDSiCCDS39703.1.
PIRiS13108.
RefSeqiNP_031558.3. NM_007532.5.
UniGeneiMm.4606.

Genome annotation databases

EnsembliENSMUST00000111742; ENSMUSP00000107371; ENSMUSG00000030268.
GeneIDi12035.
KEGGimmu:12035.
UCSCiuc009eqt.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U42443 mRNA. Translation: AAB05673.1.
X17502 mRNA. Translation: CAA35543.1. Frameshift.
BC053706 mRNA. Translation: AAH53706.1.
CCDSiCCDS39703.1.
PIRiS13108.
RefSeqiNP_031558.3. NM_007532.5.
UniGeneiMm.4606.

3D structure databases

ProteinModelPortaliP24288.
SMRiP24288. Positions 24-385.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP24288. 1 interaction.
MINTiMINT-4089144.
STRINGi10090.ENSMUSP00000032402.

PTM databases

iPTMnetiP24288.
PhosphoSiteiP24288.

Proteomic databases

EPDiP24288.
PaxDbiP24288.
PRIDEiP24288.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000111742; ENSMUSP00000107371; ENSMUSG00000030268.
GeneIDi12035.
KEGGimmu:12035.
UCSCiuc009eqt.2. mouse.

Organism-specific databases

CTDi586.
MGIiMGI:104861. Bcat1.

Phylogenomic databases

eggNOGiKOG0975. Eukaryota.
COG0115. LUCA.
HOGENOMiHOG000276704.
HOVERGENiHBG050678.
InParanoidiP24288.
KOiK00826.

Enzyme and pathway databases

ReactomeiR-MMU-70895. Branched-chain amino acid catabolism.

Miscellaneous databases

ChiTaRSiBcat1. mouse.
PROiP24288.
SOURCEiSearch...

Gene expression databases

BgeeiP24288.
CleanExiMM_BCAT1.
ExpressionAtlasiP24288. baseline and differential.
GenevisibleiP24288. MM.

Family and domain databases

InterProiIPR001544. Aminotrans_IV.
IPR018300. Aminotrans_IV_CS.
IPR005786. B_amino_transII.
[Graphical view]
PANTHERiPTHR11825. PTHR11825. 1 hit.
PfamiPF01063. Aminotran_4. 1 hit.
[Graphical view]
PIRSFiPIRSF006468. BCAT1. 1 hit.
SUPFAMiSSF56752. SSF56752. 1 hit.
TIGRFAMsiTIGR01123. ilvE_II. 1 hit.
PROSITEiPS00770. AA_TRANSFER_CLASS_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "ECA39, a conserved gene regulated by c-Myc in mice, is involved in G1/S cell cycle regulation in yeast."
    Schuldiner O., Eden A., Ben-Yosef T., Yanuka O., Simchen G., Benvenisty N.
    Proc. Natl. Acad. Sci. U.S.A. 93:7143-7148(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "A cDNA clone overexpressed and amplified in a mouse teratocarcinoma line."
    Niwa O., Kumazaki T., Tsukiyama T., Soma G., Miyajima N., Yokoro K.
    Nucleic Acids Res. 18:6709-6709(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C3H/He.
    Tissue: Mesenchymal stem cell.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain and Kidney.

Entry informationi

Entry nameiBCAT1_MOUSE
AccessioniPrimary (citable) accession number: P24288
Secondary accession number(s): Q7TMT2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.