P24282 (BOFA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sigma-K factor-processing regulatory protein BofA

Alternative name(s):
Bypass-of-forespore protein A

Gene names

Name:	bofA
Ordered Locus Names:	BSU00230

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	87 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the mediation of the intercompartmental coupling of pro-sigma K processing to events in the forespore. Inhibits SpoIVFB-processing activity until a signal has been received from the forespore. Could inhibit SpoIVFB metalloprotease activity by coordinating a zinc in the SpoIVFB active site, preventing access of a water molecule and the sequence of pro-sigma K, which are necessary for peptide bond hydrolysis to produce sigma-K. Ref.7 Ref.8 Ref.9
Subunit structure	Forms a complex with SpoIVFA and SpoIVFB localized in the mother cell membrane surrounding the forespore. Ref.8
Subcellular location	Forespore outer membrane; Multi-pass membrane protein.

Ontologies

Keywords
Biological process	Sporulation
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 87	87	Sigma-K factor-processing regulatory protein BofA		PRO_0000064969
Regions
	Topological domain	1 – 2	2	Forespore intermembrane space Probable
	Transmembrane	3 – 23	21	Helical; Probable
	Topological domain	24 – 36	13	Cytoplasmic Probable
	Transmembrane	37 – 57	21	Helical; Probable
	Topological domain	58 – 87	30	Forespore intermembrane space Probable
Sites
	Metal binding	57	1	Zinc; shared with SpoIVFB
Experimental info
	Sequence conflict	55	1	G → C in CAA34880. Ref.1
	Sequence conflict	55	1	G → C in BAA05259. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P24282 [UniParc]. Last modified June 16, 2009. Version 2. Checksum: F9B1CB8E6826B70E	FASTA	87	8,964
	10 20 30 40 50 60 MEPIFIIGII LGLVILLFLS GSAAKPLKWI GITAVKFVAG ALLLVCVNMF GGSLGIHVPI 70 80 NLVTTAISGI LGIPGIAALV VIKQFII

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, genetic characterization and DNA sequence analysis of the recM region of Bacillus subtilis." Alonso J.C., Shirahige K., Ogasawara N. Nucleic Acids Res. 18:6771-6777(1990) [PubMed: 2124672] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / YB886 / BG214.
[2]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 55.
[5]	"Characterization of bofA, a gene involved in intercompartmental regulation of pro-sigma K processing during sporulation in Bacillus subtilis." Ricca E., Cutting S.M., Losick R. J. Bacteriol. 174:3177-3184(1992) [PubMed: 1577688] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Membrane topology analysis of the Bacillus subtilis BofA protein involved in pro-sigma K processing." Varcamonti M., Marasco R., de Felice M., Sacco M. Microbiology 143:1053-1058(1997) [PubMed: 9141672] [Abstract] Cited for: TOPOLOGY.
[7]	"Role of the sporulation protein BofA in regulating activation of the Bacillus subtilis developmental transcription factor sigmaK." Resnekov O. J. Bacteriol. 181:5384-5388(1999) [PubMed: 10464210] [Abstract] Cited for: FUNCTION IN STABILIZING SPOIVFA.
[8]	"A sporulation membrane protein tethers the pro-sigmaK processing enzyme to its inhibitor and dictates its subcellular localization." Rudner D.Z., Losick R. Genes Dev. 16:1007-1018(2002) [PubMed: 11959848] [Abstract] Cited for: SUBUNIT, FUNCTION.
[9]	"BofA protein inhibits intramembrane proteolysis of pro-sigmaK in an intercompartmental signaling pathway during Bacillus subtilis sporulation." Zhou R., Kroos L. Proc. Natl. Acad. Sci. U.S.A. 101:6385-6390(2004) [PubMed: 15087499] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X17014 Genomic DNA. Translation: CAA34880.1. D26185 Genomic DNA. Translation: BAA05259.1. AL009126 Genomic DNA. Translation: CAB11799.2.
PIR	B41869.
RefSeq	NP_387904.2. NC_000964.3.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000003155; EBBACP00000003155; EBBACG00000003148.
GeneID	937025.
GenomeReviews	Gene locus BSU00230 in contig AL009126_GR.
KEGG	bsu:BSU00230.
NMPDR	fig|224308.1.peg.23.
PATRIC	18971507. VBIBacSub10457_0024.
Organism-specific databases
GenoList	BSU00230. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000002468.
HOGENOM	HBG727428.
ProtClustDB	CLSK886549.
Enzyme and pathway databases
BioCyc	BSUB:BSU00230-MONOMER.
Family and domain databases
InterPro	IPR010001. BofA. [Graphical view]
KO	K06317.
Pfam	PF07441. BofA. 1 hit. [Graphical view]
TIGRFAMs	TIGR02862. Spore_BofA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BOFA_BACSU

Accession

Primary (citable) accession number: P24282

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	June 16, 2009
Last modified:	January 25, 2012
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program