SEC14 cytosolic factor - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P24280 (SEC14_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 126. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
SEC14 cytosolic factor

Alternative name(s):
Phosphatidylinositol/phosphatidylcholine transfer protein
Short name=PI/PC TP

Gene names

Name:	SEC14
Synonyms:	PIT1
Ordered Locus Names:	YMR079W
ORF Names:	YM9582.04

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for transport of secretory proteins from the Golgi complex. Catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes in vitro. Essential for viability and secretion.
Subcellular location	Golgi apparatus membrane; Peripheral membrane protein.
Miscellaneous	Present with 84300 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 CRAL-TRIO domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Golgi apparatus Membrane
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to plasma membrane protein transport Inferred from mutant phenotype PubMed 10848624 PubMed 19129178 PubMed 6754086. Source: SGD Golgi to vacuole transport Inferred from mutant phenotype PubMed 18753406. Source: SGD Golgi vesicle budding Inferred from direct assay PubMed 9553090 PubMed 9736710. Source: SGD ascospore formation Inferred from mutant phenotype PubMed 14528019. Source: SGD negative regulation of phosphatidylcholine biosynthetic process Inferred from mutant phenotype PubMed 7816798. Source: SGD negative regulation of phosphatidylglycerol biosynthetic process Inferred from mutant phenotype PubMed 12869188. Source: SGD phosphatidylinositol metabolic process Inferred from mutant phenotype PubMed 10567405. Source: SGD
Cellular_component	Golgi membrane Inferred from direct assay PubMed 7816798. Source: SGD cytosol Inferred from direct assay PubMed 12869188 Ref.1. Source: SGD
Molecular_function	phosphatidylcholine transporter activity Inferred from direct assay PubMed 10848624 Ref.4. Source: SGD phosphatidylinositol transporter activity Inferred from direct assay Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PTC7	P38797	3	EBI-16535,EBI-24588

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.4

Chain

2 – 304

303

SEC14 cytosolic factor

PRO_0000210744

Regions

Domain

99 – 272

174

CRAL-TRIO

Amino acid modifications

Modified residue

302

Phosphoserine Ref.7

Secondary structure

304

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P24280 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: CCB69404A8A0963B
Show »

FASTA

304

34,901

        10         20         30         40         50         60 
MVTQQEKEFL ESYPQNCPPD ALPGTPGNLD SAQEKALAEL RKLLEDAGFI ERLDDSTLLR 

        70         80         90        100        110        120 
FLRARKFDVQ LAKEMFENCE KWRKDYGTDT ILQDFHYDEK PLIAKFYPQY YHKTDKDGRP 

       130        140        150        160        170        180 
VYFEELGAVN LHEMNKVTSE ERMLKNLVWE YESVVQYRLP ACSRAAGHLV ETSCTIMDLK 

       190        200        210        220        230        240 
GISISSAYSV MSYVREASYI SQNYYPERMG KFYIINAPFG FSTAFRLFKP FLDPVTVSKI 

       250        260        270        280        290        300 
FILGSSYQKE LLKQIPAENL PVKFGGKSEV DESKGGLYLS DIGPWRDPKY IGPEGEAPEA 


FSMK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Saccharomyces cerevisiae SEC14 gene encodes a cytosolic factor that is required for transport of secretory proteins from the yeast Golgi complex." Bankaitis V.A., Malehorn D.E., Emr S.D., Greene R. J. Cell Biol. 108:1271-1281(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CTY1-1A.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The gene encoding the phosphatidylinositol transfer protein is essential for cell growth." Aitken J.F., van Heusden G.P.H., Temkin M., Dowhan W. J. Biol. Chem. 265:4711-4717(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-31.
[5]	"An essential role for a phospholipid transfer protein in yeast Golgi function." Bankaitis V.A., Aitken J.R., Cleves A.E., Dowhan W. Nature 347:561-562(1990) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302, MASS SPECTROMETRY.
[8]	"Crystal structure of the Saccharomyces cerevisiae phosphatidylinositol-transfer protein." Sha B., Phillips S.E., Bankaitis V.A., Luo M. Nature 391:506-510(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 4-299.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X15483 Genomic DNA. Translation: CAA33511.1.
Z49259 Genomic DNA. Translation: CAA89225.1.
BK006946 Genomic DNA. Translation: DAA09977.1.

PIR

A30106.

RefSeq

NP_013796.1. NM_001182578.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AUA	X-ray	2.50	A	4-299	[»]

ProteinModelPortal

P24280.

SMR

P24280. Positions 4-299.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1610N.

IntAct

P24280. 8 interactions.

MINT

MINT-393005.

STRING

4932.YMR079W.

Proteomic databases

PaxDb

P24280.

PeptideAtlas

P24280.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YMR079W; YMR079W; YMR079W.

GeneID

855103.

KEGG

sce:YMR079W.

Organism-specific databases

SGD

S000004684. SEC14.

Phylogenomic databases

eggNOG

NOG309458.

GeneTree

ENSGT00550000074580.

HOGENOM

HOG000196557.

OMA

INAPWGF.

OrthoDB

EOG408RHZ.

Gene expression databases

Genevestigator

P24280.

GermOnline

YMR079W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

3.40.525.10. 1 hit.

InterPro

IPR001071. CRAL-bd_toc_tran.
IPR001251. CRAL-TRIO_dom.
IPR011074. CRAL/TRIO_N_dom.
[Graphical view]

Pfam

PF00650. CRAL_TRIO. 1 hit.
PF03765. CRAL_TRIO_N. 1 hit.
[Graphical view]

PRINTS

PR00180. CRETINALDHBP.

SMART

SM01100. CRAL_TRIO_N. 1 hit.
SM00516. SEC14. 1 hit.
[Graphical view]

SUPFAM

SSF52087. CRAL_TRIO_C. 1 hit.
SSF46938. Sec14p_like_N. 1 hit.

PROSITE

PS50191. CRAL_TRIO. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P24280.

NextBio

978428.

Entry information

Entry name

SEC14_YEAST

Accession

Primary (citable) accession number: P24280
Secondary accession number(s): D6VZQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 126 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents