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Protein

SEC14 cytosolic factor

Gene

SEC14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for transport of secretory proteins from the Golgi complex. Catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes in vitro. Essential for viability and secretion.

GO - Molecular functioni

  • phosphatidylcholine transporter activity Source: SGD
  • phosphatidylinositol transporter activity Source: SGD

GO - Biological processi

  • ascospore formation Source: SGD
  • Golgi to plasma membrane protein transport Source: SGD
  • Golgi to vacuole transport Source: SGD
  • Golgi vesicle budding Source: SGD
  • negative regulation of phosphatidylcholine biosynthetic process Source: SGD
  • negative regulation of phosphatidylglycerol biosynthetic process Source: SGD
  • phosphatidylinositol metabolic process Source: SGD
  • phospholipid transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32781-MONOMER.

Chemistry databases

SwissLipidsiSLP:000000359.

Names & Taxonomyi

Protein namesi
Recommended name:
SEC14 cytosolic factor
Alternative name(s):
Phosphatidylinositol/phosphatidylcholine transfer protein
Short name:
PI/PC TP
Gene namesi
Name:SEC14
Synonyms:PIT1
Ordered Locus Names:YMR079W
ORF Names:YM9582.04
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR079W.
SGDiS000004684. SEC14.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytosol Source: SGD
  • Golgi apparatus Source: SGD
  • Golgi membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002107442 – 304SEC14 cytosolic factorAdd BLAST303

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki42Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki84Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei302PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP24280.
PRIDEiP24280.

PTM databases

iPTMnetiP24280.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PTC7P387973EBI-16535,EBI-24588

Protein-protein interaction databases

BioGridi35255. 193 interactors.
DIPiDIP-1610N.
IntActiP24280. 8 interactors.
MINTiMINT-393005.

Structurei

Secondary structure

1304
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi6 – 10Combined sources5
Turni26 – 28Combined sources3
Helixi33 – 46Combined sources14
Helixi55 – 64Combined sources10
Turni65 – 67Combined sources3
Helixi69 – 86Combined sources18
Helixi88 – 90Combined sources3
Helixi91 – 94Combined sources4
Helixi100 – 103Combined sources4
Helixi104 – 106Combined sources3
Beta strandi110 – 114Combined sources5
Beta strandi120 – 124Combined sources5
Helixi126 – 128Combined sources3
Helixi131 – 134Combined sources4
Turni135 – 137Combined sources3
Helixi140 – 156Combined sources17
Helixi158 – 166Combined sources9
Beta strandi173 – 178Combined sources6
Helixi184 – 204Combined sources21
Beta strandi209 – 216Combined sources8
Helixi219 – 228Combined sources10
Helixi229 – 231Combined sources3
Helixi234 – 237Combined sources4
Beta strandi240 – 242Combined sources3
Helixi248 – 251Combined sources4
Turni252 – 254Combined sources3
Beta strandi257 – 261Combined sources5
Helixi262 – 264Combined sources3
Helixi272 – 274Combined sources3
Helixi284 – 286Combined sources3
Helixi288 – 290Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AUAX-ray2.50A4-299[»]
ProteinModelPortaliP24280.
SMRiP24280.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24280.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini99 – 272CRAL-TRIOPROSITE-ProRule annotationAdd BLAST174

Sequence similaritiesi

Contains 1 CRAL-TRIO domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074580.
HOGENOMiHOG000196557.
InParanoidiP24280.
OMAiWDYPEKA.
OrthoDBiEOG092C43S0.

Family and domain databases

Gene3Di3.40.525.10. 1 hit.
InterProiIPR001071. CRAL-bd_toc_tran.
IPR001251. CRAL-TRIO_dom.
IPR011074. CRAL/TRIO_N_dom.
[Graphical view]
PfamiPF00650. CRAL_TRIO. 1 hit.
PF03765. CRAL_TRIO_N. 1 hit.
[Graphical view]
PRINTSiPR00180. CRETINALDHBP.
SMARTiSM01100. CRAL_TRIO_N. 1 hit.
SM00516. SEC14. 1 hit.
[Graphical view]
SUPFAMiSSF46938. SSF46938. 1 hit.
SSF52087. SSF52087. 1 hit.
PROSITEiPS50191. CRAL_TRIO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24280-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTQQEKEFL ESYPQNCPPD ALPGTPGNLD SAQEKALAEL RKLLEDAGFI
60 70 80 90 100
ERLDDSTLLR FLRARKFDVQ LAKEMFENCE KWRKDYGTDT ILQDFHYDEK
110 120 130 140 150
PLIAKFYPQY YHKTDKDGRP VYFEELGAVN LHEMNKVTSE ERMLKNLVWE
160 170 180 190 200
YESVVQYRLP ACSRAAGHLV ETSCTIMDLK GISISSAYSV MSYVREASYI
210 220 230 240 250
SQNYYPERMG KFYIINAPFG FSTAFRLFKP FLDPVTVSKI FILGSSYQKE
260 270 280 290 300
LLKQIPAENL PVKFGGKSEV DESKGGLYLS DIGPWRDPKY IGPEGEAPEA

FSMK
Length:304
Mass (Da):34,901
Last modified:January 23, 2007 - v3
Checksum:iCCB69404A8A0963B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15483 Genomic DNA. Translation: CAA33511.1.
Z49259 Genomic DNA. Translation: CAA89225.1.
BK006946 Genomic DNA. Translation: DAA09977.1.
PIRiA30106.
RefSeqiNP_013796.1. NM_001182578.1.

Genome annotation databases

EnsemblFungiiYMR079W; YMR079W; YMR079W.
GeneIDi855103.
KEGGisce:YMR079W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15483 Genomic DNA. Translation: CAA33511.1.
Z49259 Genomic DNA. Translation: CAA89225.1.
BK006946 Genomic DNA. Translation: DAA09977.1.
PIRiA30106.
RefSeqiNP_013796.1. NM_001182578.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AUAX-ray2.50A4-299[»]
ProteinModelPortaliP24280.
SMRiP24280.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35255. 193 interactors.
DIPiDIP-1610N.
IntActiP24280. 8 interactors.
MINTiMINT-393005.

Chemistry databases

SwissLipidsiSLP:000000359.

PTM databases

iPTMnetiP24280.

Proteomic databases

MaxQBiP24280.
PRIDEiP24280.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR079W; YMR079W; YMR079W.
GeneIDi855103.
KEGGisce:YMR079W.

Organism-specific databases

EuPathDBiFungiDB:YMR079W.
SGDiS000004684. SEC14.

Phylogenomic databases

GeneTreeiENSGT00550000074580.
HOGENOMiHOG000196557.
InParanoidiP24280.
OMAiWDYPEKA.
OrthoDBiEOG092C43S0.

Enzyme and pathway databases

BioCyciYEAST:G3O-32781-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP24280.
PROiP24280.

Family and domain databases

Gene3Di3.40.525.10. 1 hit.
InterProiIPR001071. CRAL-bd_toc_tran.
IPR001251. CRAL-TRIO_dom.
IPR011074. CRAL/TRIO_N_dom.
[Graphical view]
PfamiPF00650. CRAL_TRIO. 1 hit.
PF03765. CRAL_TRIO_N. 1 hit.
[Graphical view]
PRINTSiPR00180. CRETINALDHBP.
SMARTiSM01100. CRAL_TRIO_N. 1 hit.
SM00516. SEC14. 1 hit.
[Graphical view]
SUPFAMiSSF46938. SSF46938. 1 hit.
SSF52087. SSF52087. 1 hit.
PROSITEiPS50191. CRAL_TRIO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSEC14_YEAST
AccessioniPrimary (citable) accession number: P24280
Secondary accession number(s): D6VZQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 155 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 84300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.