Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P24280

- SEC14_YEAST

UniProt

P24280 - SEC14_YEAST

Protein

SEC14 cytosolic factor

Gene

SEC14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Required for transport of secretory proteins from the Golgi complex. Catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes in vitro. Essential for viability and secretion.

    GO - Molecular functioni

    1. phosphatidylcholine transporter activity Source: SGD
    2. phosphatidylinositol transporter activity Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. ascospore formation Source: SGD
    2. Golgi to plasma membrane protein transport Source: SGD
    3. Golgi to vacuole transport Source: SGD
    4. Golgi vesicle budding Source: SGD
    5. negative regulation of phosphatidylcholine biosynthetic process Source: SGD
    6. negative regulation of phosphatidylglycerol biosynthetic process Source: SGD
    7. phosphatidylinositol metabolic process Source: SGD
    8. phospholipid transport Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32781-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    SEC14 cytosolic factor
    Alternative name(s):
    Phosphatidylinositol/phosphatidylcholine transfer protein
    Short name:
    PI/PC TP
    Gene namesi
    Name:SEC14
    Synonyms:PIT1
    Ordered Locus Names:YMR079W
    ORF Names:YM9582.04
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    SGDiS000004684. SEC14.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. cytosol Source: SGD
    3. Golgi apparatus Source: SGD
    4. Golgi membrane Source: SGD

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 304303SEC14 cytosolic factorPRO_0000210744Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei302 – 3021Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP24280.
    PaxDbiP24280.
    PeptideAtlasiP24280.

    Expressioni

    Gene expression databases

    GenevestigatoriP24280.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PTC7P387973EBI-16535,EBI-24588

    Protein-protein interaction databases

    BioGridi35255. 193 interactions.
    DIPiDIP-1610N.
    IntActiP24280. 8 interactions.
    MINTiMINT-393005.
    STRINGi4932.YMR079W.

    Structurei

    Secondary structure

    1
    304
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi6 – 105
    Turni26 – 283
    Helixi33 – 4614
    Helixi55 – 6410
    Turni65 – 673
    Helixi69 – 8618
    Helixi88 – 903
    Helixi91 – 944
    Helixi100 – 1034
    Helixi104 – 1063
    Beta strandi110 – 1145
    Beta strandi120 – 1245
    Helixi126 – 1283
    Helixi131 – 1344
    Turni135 – 1373
    Helixi140 – 15617
    Helixi158 – 1669
    Beta strandi173 – 1786
    Helixi184 – 20421
    Beta strandi209 – 2168
    Helixi219 – 22810
    Helixi229 – 2313
    Helixi234 – 2374
    Beta strandi240 – 2423
    Helixi248 – 2514
    Turni252 – 2543
    Beta strandi257 – 2615
    Helixi262 – 2643
    Helixi272 – 2743
    Helixi284 – 2863
    Helixi288 – 2903

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AUAX-ray2.50A4-299[»]
    ProteinModelPortaliP24280.
    SMRiP24280. Positions 4-299.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP24280.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini99 – 272174CRAL-TRIOPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CRAL-TRIO domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG309458.
    GeneTreeiENSGT00550000074580.
    HOGENOMiHOG000196557.
    OMAiLTAMYKI.
    OrthoDBiEOG761C55.

    Family and domain databases

    Gene3Di3.40.525.10. 1 hit.
    InterProiIPR001071. CRAL-bd_toc_tran.
    IPR001251. CRAL-TRIO_dom.
    IPR011074. CRAL/TRIO_N_dom.
    [Graphical view]
    PfamiPF00650. CRAL_TRIO. 1 hit.
    PF03765. CRAL_TRIO_N. 1 hit.
    [Graphical view]
    PRINTSiPR00180. CRETINALDHBP.
    SMARTiSM01100. CRAL_TRIO_N. 1 hit.
    SM00516. SEC14. 1 hit.
    [Graphical view]
    SUPFAMiSSF46938. SSF46938. 1 hit.
    SSF52087. SSF52087. 1 hit.
    PROSITEiPS50191. CRAL_TRIO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P24280-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVTQQEKEFL ESYPQNCPPD ALPGTPGNLD SAQEKALAEL RKLLEDAGFI    50
    ERLDDSTLLR FLRARKFDVQ LAKEMFENCE KWRKDYGTDT ILQDFHYDEK 100
    PLIAKFYPQY YHKTDKDGRP VYFEELGAVN LHEMNKVTSE ERMLKNLVWE 150
    YESVVQYRLP ACSRAAGHLV ETSCTIMDLK GISISSAYSV MSYVREASYI 200
    SQNYYPERMG KFYIINAPFG FSTAFRLFKP FLDPVTVSKI FILGSSYQKE 250
    LLKQIPAENL PVKFGGKSEV DESKGGLYLS DIGPWRDPKY IGPEGEAPEA 300
    FSMK 304
    Length:304
    Mass (Da):34,901
    Last modified:January 23, 2007 - v3
    Checksum:iCCB69404A8A0963B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15483 Genomic DNA. Translation: CAA33511.1.
    Z49259 Genomic DNA. Translation: CAA89225.1.
    BK006946 Genomic DNA. Translation: DAA09977.1.
    PIRiA30106.
    RefSeqiNP_013796.1. NM_001182578.1.

    Genome annotation databases

    EnsemblFungiiYMR079W; YMR079W; YMR079W.
    GeneIDi855103.
    KEGGisce:YMR079W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15483 Genomic DNA. Translation: CAA33511.1 .
    Z49259 Genomic DNA. Translation: CAA89225.1 .
    BK006946 Genomic DNA. Translation: DAA09977.1 .
    PIRi A30106.
    RefSeqi NP_013796.1. NM_001182578.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AUA X-ray 2.50 A 4-299 [» ]
    ProteinModelPortali P24280.
    SMRi P24280. Positions 4-299.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35255. 193 interactions.
    DIPi DIP-1610N.
    IntActi P24280. 8 interactions.
    MINTi MINT-393005.
    STRINGi 4932.YMR079W.

    Proteomic databases

    MaxQBi P24280.
    PaxDbi P24280.
    PeptideAtlasi P24280.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR079W ; YMR079W ; YMR079W .
    GeneIDi 855103.
    KEGGi sce:YMR079W.

    Organism-specific databases

    SGDi S000004684. SEC14.

    Phylogenomic databases

    eggNOGi NOG309458.
    GeneTreei ENSGT00550000074580.
    HOGENOMi HOG000196557.
    OMAi LTAMYKI.
    OrthoDBi EOG761C55.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32781-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P24280.
    NextBioi 978428.

    Gene expression databases

    Genevestigatori P24280.

    Family and domain databases

    Gene3Di 3.40.525.10. 1 hit.
    InterProi IPR001071. CRAL-bd_toc_tran.
    IPR001251. CRAL-TRIO_dom.
    IPR011074. CRAL/TRIO_N_dom.
    [Graphical view ]
    Pfami PF00650. CRAL_TRIO. 1 hit.
    PF03765. CRAL_TRIO_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00180. CRETINALDHBP.
    SMARTi SM01100. CRAL_TRIO_N. 1 hit.
    SM00516. SEC14. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46938. SSF46938. 1 hit.
    SSF52087. SSF52087. 1 hit.
    PROSITEi PS50191. CRAL_TRIO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Saccharomyces cerevisiae SEC14 gene encodes a cytosolic factor that is required for transport of secretory proteins from the yeast Golgi complex."
      Bankaitis V.A., Malehorn D.E., Emr S.D., Greene R.
      J. Cell Biol. 108:1271-1281(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: CTY1-1A.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "The gene encoding the phosphatidylinositol transfer protein is essential for cell growth."
      Aitken J.F., van Heusden G.P.H., Temkin M., Dowhan W.
      J. Biol. Chem. 265:4711-4717(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-31.
    5. "An essential role for a phospholipid transfer protein in yeast Golgi function."
      Bankaitis V.A., Aitken J.R., Cleves A.E., Dowhan W.
      Nature 347:561-562(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Crystal structure of the Saccharomyces cerevisiae phosphatidylinositol-transfer protein."
      Sha B., Phillips S.E., Bankaitis V.A., Luo M.
      Nature 391:506-510(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 4-299.

    Entry informationi

    Entry nameiSEC14_YEAST
    AccessioniPrimary (citable) accession number: P24280
    Secondary accession number(s): D6VZQ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 137 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 84300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3