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P24280 (SEC14_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
SEC14 cytosolic factor
Alternative name(s):
Phosphatidylinositol/phosphatidylcholine transfer protein
Short name=PI/PC TP
Gene names
Name:SEC14
Synonyms:PIT1
Ordered Locus Names:YMR079W
ORF Names:YM9582.04
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length304 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for transport of secretory proteins from the Golgi complex. Catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes in vitro. Essential for viability and secretion.

Subcellular location

Golgi apparatus membrane; Peripheral membrane protein.

Miscellaneous

Present with 84300 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 CRAL-TRIO domain.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentGolgi apparatus
Membrane
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processGolgi to plasma membrane protein transport

Inferred from mutant phenotype PubMed 10848624PubMed 19129178PubMed 6754086. Source: SGD

Golgi to vacuole transport

Inferred from mutant phenotype PubMed 18753406. Source: SGD

Golgi vesicle budding

Inferred from direct assay PubMed 9553090PubMed 9736710. Source: SGD

ascospore formation

Inferred from mutant phenotype PubMed 14528019. Source: SGD

negative regulation of phosphatidylcholine biosynthetic process

Inferred from direct assay PubMed 7816798. Source: SGD

negative regulation of phosphatidylglycerol biosynthetic process

Inferred from mutant phenotype PubMed 12869188. Source: SGD

phosphatidylinositol metabolic process

Inferred from mutant phenotype PubMed 10567405. Source: SGD

phospholipid transport

Inferred from direct assay PubMed 10848624Ref.5Ref.4. Source: SGD

   Cellular_componentGolgi apparatus

Inferred from direct assay PubMed 1997207. Source: SGD

Golgi membrane

Inferred from direct assay PubMed 7816798. Source: SGD

cytoplasm

Inferred from direct assay PubMed 1997207. Source: SGD

cytosol

Inferred from direct assay PubMed 12869188Ref.1. Source: SGD

   Molecular_functionphosphatidylcholine transporter activity

Inferred from direct assay PubMed 10488334PubMed 10848624Ref.4. Source: SGD

phosphatidylinositol transporter activity

Inferred from direct assay PubMed 10848624Ref.5Ref.4. Source: SGD

protein binding

Inferred from physical interaction PubMed 18719252PubMed 20489023. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PTC7P387973EBI-16535,EBI-24588

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.4
Chain2 – 304303SEC14 cytosolic factor
PRO_0000210744

Regions

Domain99 – 272174CRAL-TRIO

Amino acid modifications

Modified residue3021Phosphoserine Ref.7 Ref.8

Secondary structure

........................................................ 304
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P24280 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: CCB69404A8A0963B

FASTA30434,901
        10         20         30         40         50         60 
MVTQQEKEFL ESYPQNCPPD ALPGTPGNLD SAQEKALAEL RKLLEDAGFI ERLDDSTLLR 

        70         80         90        100        110        120 
FLRARKFDVQ LAKEMFENCE KWRKDYGTDT ILQDFHYDEK PLIAKFYPQY YHKTDKDGRP 

       130        140        150        160        170        180 
VYFEELGAVN LHEMNKVTSE ERMLKNLVWE YESVVQYRLP ACSRAAGHLV ETSCTIMDLK 

       190        200        210        220        230        240 
GISISSAYSV MSYVREASYI SQNYYPERMG KFYIINAPFG FSTAFRLFKP FLDPVTVSKI 

       250        260        270        280        290        300 
FILGSSYQKE LLKQIPAENL PVKFGGKSEV DESKGGLYLS DIGPWRDPKY IGPEGEAPEA 


FSMK 

« Hide

References

« Hide 'large scale' references
[1]"The Saccharomyces cerevisiae SEC14 gene encodes a cytosolic factor that is required for transport of secretory proteins from the yeast Golgi complex."
Bankaitis V.A., Malehorn D.E., Emr S.D., Greene R.
J. Cell Biol. 108:1271-1281(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: CTY1-1A.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"The gene encoding the phosphatidylinositol transfer protein is essential for cell growth."
Aitken J.F., van Heusden G.P.H., Temkin M., Dowhan W.
J. Biol. Chem. 265:4711-4717(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-31.
[5]"An essential role for a phospholipid transfer protein in yeast Golgi function."
Bankaitis V.A., Aitken J.R., Cleves A.E., Dowhan W.
Nature 347:561-562(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Crystal structure of the Saccharomyces cerevisiae phosphatidylinositol-transfer protein."
Sha B., Phillips S.E., Bankaitis V.A., Luo M.
Nature 391:506-510(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 4-299.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X15483 Genomic DNA. Translation: CAA33511.1.
Z49259 Genomic DNA. Translation: CAA89225.1.
BK006946 Genomic DNA. Translation: DAA09977.1.
PIRA30106.
RefSeqNP_013796.1. NM_001182578.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1AUAX-ray2.50A4-299[»]
ProteinModelPortalP24280.
SMRP24280. Positions 4-299.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35255. 193 interactions.
DIPDIP-1610N.
IntActP24280. 8 interactions.
MINTMINT-393005.
STRING4932.YMR079W.

Proteomic databases

MaxQBP24280.
PaxDbP24280.
PeptideAtlasP24280.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR079W; YMR079W; YMR079W.
GeneID855103.
KEGGsce:YMR079W.

Organism-specific databases

SGDS000004684. SEC14.

Phylogenomic databases

eggNOGNOG309458.
GeneTreeENSGT00550000074580.
HOGENOMHOG000196557.
OMALTAMYKI.
OrthoDBEOG761C55.

Enzyme and pathway databases

BioCycYEAST:G3O-32781-MONOMER.

Gene expression databases

GenevestigatorP24280.

Family and domain databases

Gene3D3.40.525.10. 1 hit.
InterProIPR001071. CRAL-bd_toc_tran.
IPR001251. CRAL-TRIO_dom.
IPR011074. CRAL/TRIO_N_dom.
[Graphical view]
PfamPF00650. CRAL_TRIO. 1 hit.
PF03765. CRAL_TRIO_N. 1 hit.
[Graphical view]
PRINTSPR00180. CRETINALDHBP.
SMARTSM01100. CRAL_TRIO_N. 1 hit.
SM00516. SEC14. 1 hit.
[Graphical view]
SUPFAMSSF46938. SSF46938. 1 hit.
SSF52087. SSF52087. 1 hit.
PROSITEPS50191. CRAL_TRIO. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP24280.
NextBio978428.

Entry information

Entry nameSEC14_YEAST
AccessionPrimary (citable) accession number: P24280
Secondary accession number(s): D6VZQ3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references