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P24280

- SEC14_YEAST

UniProt

P24280 - SEC14_YEAST

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Protein
SEC14 cytosolic factor
Gene
SEC14, PIT1, YMR079W, YM9582.04
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for transport of secretory proteins from the Golgi complex. Catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes in vitro. Essential for viability and secretion.

GO - Molecular functioni

  1. phosphatidylcholine transporter activity Source: SGD
  2. phosphatidylinositol transporter activity Source: SGD
  3. protein binding Source: IntAct

GO - Biological processi

  1. Golgi to plasma membrane protein transport Source: SGD
  2. Golgi to vacuole transport Source: SGD
  3. Golgi vesicle budding Source: SGD
  4. ascospore formation Source: SGD
  5. negative regulation of phosphatidylcholine biosynthetic process Source: SGD
  6. negative regulation of phosphatidylglycerol biosynthetic process Source: SGD
  7. phosphatidylinositol metabolic process Source: SGD
  8. phospholipid transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32781-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
SEC14 cytosolic factor
Alternative name(s):
Phosphatidylinositol/phosphatidylcholine transfer protein
Short name:
PI/PC TP
Gene namesi
Name:SEC14
Synonyms:PIT1
Ordered Locus Names:YMR079W
ORF Names:YM9582.04
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

SGDiS000004684. SEC14.

Subcellular locationi

GO - Cellular componenti

  1. Golgi apparatus Source: SGD
  2. Golgi membrane Source: SGD
  3. cytoplasm Source: SGD
  4. cytosol Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 304303SEC14 cytosolic factor
PRO_0000210744Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei302 – 3021Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP24280.
PaxDbiP24280.
PeptideAtlasiP24280.

Expressioni

Gene expression databases

GenevestigatoriP24280.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PTC7P387973EBI-16535,EBI-24588

Protein-protein interaction databases

BioGridi35255. 193 interactions.
DIPiDIP-1610N.
IntActiP24280. 8 interactions.
MINTiMINT-393005.
STRINGi4932.YMR079W.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi6 – 105
Turni26 – 283
Helixi33 – 4614
Helixi55 – 6410
Turni65 – 673
Helixi69 – 8618
Helixi88 – 903
Helixi91 – 944
Helixi100 – 1034
Helixi104 – 1063
Beta strandi110 – 1145
Beta strandi120 – 1245
Helixi126 – 1283
Helixi131 – 1344
Turni135 – 1373
Helixi140 – 15617
Helixi158 – 1669
Beta strandi173 – 1786
Helixi184 – 20421
Beta strandi209 – 2168
Helixi219 – 22810
Helixi229 – 2313
Helixi234 – 2374
Beta strandi240 – 2423
Helixi248 – 2514
Turni252 – 2543
Beta strandi257 – 2615
Helixi262 – 2643
Helixi272 – 2743
Helixi284 – 2863
Helixi288 – 2903

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AUAX-ray2.50A4-299[»]
ProteinModelPortaliP24280.
SMRiP24280. Positions 4-299.

Miscellaneous databases

EvolutionaryTraceiP24280.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini99 – 272174CRAL-TRIO
Add
BLAST

Sequence similaritiesi

Contains 1 CRAL-TRIO domain.

Phylogenomic databases

eggNOGiNOG309458.
GeneTreeiENSGT00550000074580.
HOGENOMiHOG000196557.
OMAiLTAMYKI.
OrthoDBiEOG761C55.

Family and domain databases

Gene3Di3.40.525.10. 1 hit.
InterProiIPR001071. CRAL-bd_toc_tran.
IPR001251. CRAL-TRIO_dom.
IPR011074. CRAL/TRIO_N_dom.
[Graphical view]
PfamiPF00650. CRAL_TRIO. 1 hit.
PF03765. CRAL_TRIO_N. 1 hit.
[Graphical view]
PRINTSiPR00180. CRETINALDHBP.
SMARTiSM01100. CRAL_TRIO_N. 1 hit.
SM00516. SEC14. 1 hit.
[Graphical view]
SUPFAMiSSF46938. SSF46938. 1 hit.
SSF52087. SSF52087. 1 hit.
PROSITEiPS50191. CRAL_TRIO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24280-1 [UniParc]FASTAAdd to Basket

« Hide

MVTQQEKEFL ESYPQNCPPD ALPGTPGNLD SAQEKALAEL RKLLEDAGFI    50
ERLDDSTLLR FLRARKFDVQ LAKEMFENCE KWRKDYGTDT ILQDFHYDEK 100
PLIAKFYPQY YHKTDKDGRP VYFEELGAVN LHEMNKVTSE ERMLKNLVWE 150
YESVVQYRLP ACSRAAGHLV ETSCTIMDLK GISISSAYSV MSYVREASYI 200
SQNYYPERMG KFYIINAPFG FSTAFRLFKP FLDPVTVSKI FILGSSYQKE 250
LLKQIPAENL PVKFGGKSEV DESKGGLYLS DIGPWRDPKY IGPEGEAPEA 300
FSMK 304
Length:304
Mass (Da):34,901
Last modified:January 23, 2007 - v3
Checksum:iCCB69404A8A0963B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15483 Genomic DNA. Translation: CAA33511.1.
Z49259 Genomic DNA. Translation: CAA89225.1.
BK006946 Genomic DNA. Translation: DAA09977.1.
PIRiA30106.
RefSeqiNP_013796.1. NM_001182578.1.

Genome annotation databases

EnsemblFungiiYMR079W; YMR079W; YMR079W.
GeneIDi855103.
KEGGisce:YMR079W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15483 Genomic DNA. Translation: CAA33511.1 .
Z49259 Genomic DNA. Translation: CAA89225.1 .
BK006946 Genomic DNA. Translation: DAA09977.1 .
PIRi A30106.
RefSeqi NP_013796.1. NM_001182578.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1AUA X-ray 2.50 A 4-299 [» ]
ProteinModelPortali P24280.
SMRi P24280. Positions 4-299.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35255. 193 interactions.
DIPi DIP-1610N.
IntActi P24280. 8 interactions.
MINTi MINT-393005.
STRINGi 4932.YMR079W.

Proteomic databases

MaxQBi P24280.
PaxDbi P24280.
PeptideAtlasi P24280.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR079W ; YMR079W ; YMR079W .
GeneIDi 855103.
KEGGi sce:YMR079W.

Organism-specific databases

SGDi S000004684. SEC14.

Phylogenomic databases

eggNOGi NOG309458.
GeneTreei ENSGT00550000074580.
HOGENOMi HOG000196557.
OMAi LTAMYKI.
OrthoDBi EOG761C55.

Enzyme and pathway databases

BioCyci YEAST:G3O-32781-MONOMER.

Miscellaneous databases

EvolutionaryTracei P24280.
NextBioi 978428.

Gene expression databases

Genevestigatori P24280.

Family and domain databases

Gene3Di 3.40.525.10. 1 hit.
InterProi IPR001071. CRAL-bd_toc_tran.
IPR001251. CRAL-TRIO_dom.
IPR011074. CRAL/TRIO_N_dom.
[Graphical view ]
Pfami PF00650. CRAL_TRIO. 1 hit.
PF03765. CRAL_TRIO_N. 1 hit.
[Graphical view ]
PRINTSi PR00180. CRETINALDHBP.
SMARTi SM01100. CRAL_TRIO_N. 1 hit.
SM00516. SEC14. 1 hit.
[Graphical view ]
SUPFAMi SSF46938. SSF46938. 1 hit.
SSF52087. SSF52087. 1 hit.
PROSITEi PS50191. CRAL_TRIO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Saccharomyces cerevisiae SEC14 gene encodes a cytosolic factor that is required for transport of secretory proteins from the yeast Golgi complex."
    Bankaitis V.A., Malehorn D.E., Emr S.D., Greene R.
    J. Cell Biol. 108:1271-1281(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: CTY1-1A.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The gene encoding the phosphatidylinositol transfer protein is essential for cell growth."
    Aitken J.F., van Heusden G.P.H., Temkin M., Dowhan W.
    J. Biol. Chem. 265:4711-4717(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-31.
  5. "An essential role for a phospholipid transfer protein in yeast Golgi function."
    Bankaitis V.A., Aitken J.R., Cleves A.E., Dowhan W.
    Nature 347:561-562(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Crystal structure of the Saccharomyces cerevisiae phosphatidylinositol-transfer protein."
    Sha B., Phillips S.E., Bankaitis V.A., Luo M.
    Nature 391:506-510(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 4-299.

Entry informationi

Entry nameiSEC14_YEAST
AccessioniPrimary (citable) accession number: P24280
Secondary accession number(s): D6VZQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 84300 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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