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Protein

DNA replication licensing factor MCM3

Gene

MCM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity. Necessary for cell growth.2 Publications

Miscellaneous

Present with 35100 molecules/cell in log phase SD medium.1 Publication
Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. The MCM2-7 hexamer is the proposed physiological active complex.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi409 – 416ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • chromatin binding Source: SGD
  • DNA replication origin binding Source: SGD
  • helicase activity Source: UniProtKB-KW
  • MCM complex binding Source: SGD

GO - Biological processi

  • chromatin silencing at silent mating-type cassette Source: SGD
  • chromatin silencing at telomere Source: SGD
  • DNA duplex unwinding Source: GOC
  • DNA replication initiation Source: SGD
  • DNA strand elongation involved in DNA replication Source: SGD
  • double-strand break repair via break-induced replication Source: SGD
  • mitotic DNA replication initiation Source: SGD
  • pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30154-MONOMER
ReactomeiR-SCE-68949 Orc1 removal from chromatin
R-SCE-68962 Activation of the pre-replicative complex
R-SCE-69052 Switching of origins to a post-replicative state

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM3 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance protein 3
Gene namesi
Name:MCM3
Ordered Locus Names:YEL032W
ORF Names:SYGP-ORF23
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL032W
SGDiS000000758 MCM3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi415K → A: No effect on MCM2-7 complex helicase activity. Loss of MCM2-7 complex helicase activity; when associated with MCM5 A-422. Reduces MCM2-7 complex helicase activity; when associated with MCM2 A-549. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001940991 – 971DNA replication licensing factor MCM3Add BLAST971

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei761PhosphoserineCombined sources1
Modified residuei777PhosphoserineCombined sources1
Modified residuei781PhosphoserineCombined sources1
Modified residuei868PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP24279
PaxDbiP24279
PRIDEiP24279

PTM databases

iPTMnetiP24279

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5; loaded onto DNA, forms a head-head double hexamer. Interacts with CSM1.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36697, 393 interactors
DIPiDIP-2407N
IntActiP24279, 35 interactors
MINTiP24279
STRINGi4932.YEL032W

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JA8electron microscopy3.8031-971[»]
3JC5electron microscopy4.7031-971[»]
3JC6electron microscopy3.7031-971[»]
3JC7electron microscopy4.8031-971[»]
5BK4electron microscopy3.903/B1-971[»]
5H7Ielectron microscopy7.1031-971[»]
5U8Selectron microscopy6.1031-971[»]
5U8Telectron microscopy4.9031-971[»]
5UDBelectron microscopy3.9031-971[»]
5V8Felectron microscopy3.9031-971[»]
5XF8electron microscopy7.1031-971[»]
6F0Lelectron microscopy4.773/B1-971[»]
ProteinModelPortaliP24279
SMRiP24279
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini359 – 566MCMAdd BLAST208

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi541 – 544Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated

Phylogenomic databases

GeneTreeiENSGT00900000141082
HOGENOMiHOG000224126
InParanoidiP24279
KOiK02541
OMAiRNDQNTK
OrthoDBiEOG092C0VUM

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR031327 MCM
IPR008046 Mcm3
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01659 MCMPROTEIN3
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit

Sequencei

Sequence statusi: Complete.

P24279-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGSTGFDGD ATTFFAPDAV FGDRVRRFQE FLDTFTSYRD SVRSIQVYNS
60 70 80 90 100
NNAANYNDDQ DDADERDLLG DDDGDDLEKE KKAASSTSLN ILPHRIIISL
110 120 130 140 150
DDLREFDRSF WSGILVEPAY FIPPAEKALT DLADSMDDVP HPNASAVSSR
160 170 180 190 200
HPWKLSFKGS FGAHALSPRT LTAQHLNKLV SVEGIVTKTS LVRPKLIRSV
210 220 230 240 250
HYAAKTGRFH YRDYTDATTT LTTRIPTPAI YPTEDTEGNK LTTEYGYSTF
260 270 280 290 300
IDHQRITVQE MPEMAPAGQL PRSIDVILDD DLVDKTKPGD RVNVVGVFKS
310 320 330 340 350
LGAGGMNQSN SNTLIGFKTL ILGNTVYPLH ARSTGVAARQ MLTDFDIRNI
360 370 380 390 400
NKLSKKKDIF DILSQSLAPS IYGHDHIKKA ILLMLMGGVE KNLENGSHLR
410 420 430 440 450
GDINILMVGD PSTAKSQLLR FVLNTASLAI ATTGRGSSGV GLTAAVTTDR
460 470 480 490 500
ETGERRLEAG AMVLADRGVV CIDEFDKMTD VDRVAIHEVM EQQTVTIAKA
510 520 530 540 550
GIHTTLNARC SVIAAANPVF GQYDVNRDPH QNIALPDSLL SRFDLLFVVT
560 570 580 590 600
DDINEIRDRS ISEHVLRTHR YLPPGYLEGE PVRERLNLSL AVGEDADINP
610 620 630 640 650
EEHSNSGAGV ENEGEDDEDH VFEKFNPLLQ AGAKLAKNKG NYNGTEIPKL
660 670 680 690 700
VTIPFLRKYV QYAKERVIPQ LTQEAINVIV KNYTDLRNDD NTKKSPITAR
710 720 730 740 750
TLETLIRLAT AHAKVRLSKT VNKVDAKVAA NLLRFALLGE DIGNDIDEEE
760 770 780 790 800
SEYEEALSKR SPQKSPKKRQ RVRQPASNSG SPIKSTPRRS TASSVNATPS
810 820 830 840 850
SARRILRFQD DEQNAGEDDN DIMSPLPADE EAELQRRLQL GLRVSPRRRE
860 870 880 890 900
HLHAPEEGSS GPLTEVGTPR LPNVSSAGQD DEQQQSVISF DNVEPGTIST
910 920 930 940 950
GRLSLISGII ARLMQTEIFE EESYPVASLF ERINEELPEE EKFSAQEYLA
960 970
GLKIMSDRNN LMVADDKVWR V
Length:971
Mass (Da):107,518
Last modified:March 1, 1992 - v1
Checksum:i43DD4DACAF4456DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53540 Genomic DNA Translation: CAA37616.1
U18779 Genomic DNA Translation: AAB65010.1
BK006939 Genomic DNA Translation: DAA07621.1
PIRiA36376
RefSeqiNP_010882.1, NM_001178847.1

Genome annotation databases

EnsemblFungiiYEL032W; YEL032W; YEL032W
GeneIDi856680
KEGGisce:YEL032W

Similar proteinsi

Entry informationi

Entry nameiMCM3_YEAST
AccessioniPrimary (citable) accession number: P24279
Secondary accession number(s): D3DLL7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: May 23, 2018
This is version 182 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names

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