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UniProtKB - P24279 (MCM3_YEAST)

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Protein

DNA replication licensing factor MCM3

Gene

MCM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity. Necessary for cell growth.2 Publications

Miscellaneous

Present with 35100 molecules/cell in log phase SD medium.1 Publication
Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. The MCM2-7 hexamer is the proposed physiological active complex.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi409 – 416ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • chromatin binding Source: SGD
  • DNA replication origin binding Source: SGD
  • helicase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  • chromatin silencing at silent mating-type cassette Source: SGD
  • chromatin silencing at telomere Source: SGD
  • DNA duplex unwinding Source: GOC
  • DNA replication initiation Source: SGD
  • DNA strand elongation involved in DNA replication Source: SGD
  • double-strand break repair via break-induced replication Source: SGD
  • mitotic DNA replication initiation Source: SGD
  • pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD
Complete GO annotation...

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-30154-MONOMER.
ReactomeiR-SCE-176974. Unwinding of DNA.
R-SCE-68949. Orc1 removal from chromatin.
R-SCE-68962. Activation of the pre-replicative complex.
R-SCE-69052. Switching of origins to a post-replicative state.
R-SCE-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM3 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance protein 3
Gene namesi
Name:MCM3
Ordered Locus Names:YEL032W
ORF Names:SYGP-ORF23
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›
Proteomesi
  • UP000002311 Componenti: Chromosome V

Organism-specific databases

EuPathDBiFungiDB:YEL032W.
SGDiS000000758. MCM3.

Subcellular locationi

GO - Cellular componenti

  • chromosome, telomeric region Source: GOC
  • CMG complex Source: SGD
  • cytoplasm Source: SGD
  • DNA replication preinitiation complex Source: SGD
  • MCM complex Source: SGD
  • nuclear pre-replicative complex Source: SGD
  • nucleoplasm Source: Reactome
  • nucleus Source: SGD
  • replication fork protection complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi415K → A: No effect on MCM2-7 complex helicase activity. Loss of MCM2-7 complex helicase activity; when associated with MCM5 A-422. Reduces MCM2-7 complex helicase activity; when associated with MCM2 A-549. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001940991 – 971DNA replication licensing factor MCM3Add BLAST971

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei761PhosphoserineCombined sources1
Modified residuei777PhosphoserineCombined sources1
Modified residuei781PhosphoserineCombined sources1
Modified residuei868PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP24279.
PRIDEiP24279.

PTM databases

iPTMnetiP24279.

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5; loaded onto DNA, forms a head-head double hexamer. Interacts with CSM1.3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36697. 379 interactors.
DIPiDIP-2407N.
IntActiP24279. 30 interactors.
MINTiMINT-699197.
STRINGi4932.YEL032W.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JA8electron microscopy3.8031-971[»]
3JC5electron microscopy4.7031-971[»]
3JC6electron microscopy3.7031-971[»]
3JC7electron microscopy4.8031-971[»]
5H7Ielectron microscopy7.1031-971[»]
5U8Selectron microscopy6.1031-971[»]
5U8Telectron microscopy4.9031-971[»]
5UDBelectron microscopy3.9031-971[»]
5V8Felectron microscopy3.9031-971[»]
5XF8electron microscopy7.1031-971[»]
ProteinModelPortaliP24279.
SMRiP24279.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini359 – 566MCMAdd BLAST208

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi541 – 544Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000075022.
HOGENOMiHOG000224126.
InParanoidiP24279.
KOiK02541.
OMAiRNDQNTK.
OrthoDBiEOG092C0VUM.

Family and domain databases

InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR008046. Mcm3.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR033762. MCM_OB.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00493. MCM. 1 hit.
PF17207. MCM_OB. 1 hit.
PRINTSiPR01657. MCMFAMILY.
PR01659. MCMPROTEIN3.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiView protein in PROSITE
PS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P24279-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGSTGFDGD ATTFFAPDAV FGDRVRRFQE FLDTFTSYRD SVRSIQVYNS 
        60         70         80         90        100
NNAANYNDDQ DDADERDLLG DDDGDDLEKE KKAASSTSLN ILPHRIIISL 
       110        120        130        140        150
DDLREFDRSF WSGILVEPAY FIPPAEKALT DLADSMDDVP HPNASAVSSR 
       160        170        180        190        200
HPWKLSFKGS FGAHALSPRT LTAQHLNKLV SVEGIVTKTS LVRPKLIRSV 
       210        220        230        240        250
HYAAKTGRFH YRDYTDATTT LTTRIPTPAI YPTEDTEGNK LTTEYGYSTF 
       260        270        280        290        300
IDHQRITVQE MPEMAPAGQL PRSIDVILDD DLVDKTKPGD RVNVVGVFKS 
       310        320        330        340        350
LGAGGMNQSN SNTLIGFKTL ILGNTVYPLH ARSTGVAARQ MLTDFDIRNI 
       360        370        380        390        400
NKLSKKKDIF DILSQSLAPS IYGHDHIKKA ILLMLMGGVE KNLENGSHLR 
       410        420        430        440        450
GDINILMVGD PSTAKSQLLR FVLNTASLAI ATTGRGSSGV GLTAAVTTDR 
       460        470        480        490        500
ETGERRLEAG AMVLADRGVV CIDEFDKMTD VDRVAIHEVM EQQTVTIAKA 
       510        520        530        540        550
GIHTTLNARC SVIAAANPVF GQYDVNRDPH QNIALPDSLL SRFDLLFVVT 
       560        570        580        590        600
DDINEIRDRS ISEHVLRTHR YLPPGYLEGE PVRERLNLSL AVGEDADINP 
       610        620        630        640        650
EEHSNSGAGV ENEGEDDEDH VFEKFNPLLQ AGAKLAKNKG NYNGTEIPKL 
       660        670        680        690        700
VTIPFLRKYV QYAKERVIPQ LTQEAINVIV KNYTDLRNDD NTKKSPITAR 
       710        720        730        740        750
TLETLIRLAT AHAKVRLSKT VNKVDAKVAA NLLRFALLGE DIGNDIDEEE 
       760        770        780        790        800
SEYEEALSKR SPQKSPKKRQ RVRQPASNSG SPIKSTPRRS TASSVNATPS 
       810        820        830        840        850
SARRILRFQD DEQNAGEDDN DIMSPLPADE EAELQRRLQL GLRVSPRRRE 
       860        870        880        890        900
HLHAPEEGSS GPLTEVGTPR LPNVSSAGQD DEQQQSVISF DNVEPGTIST 
       910        920        930        940        950
GRLSLISGII ARLMQTEIFE EESYPVASLF ERINEELPEE EKFSAQEYLA 
       960        970 
GLKIMSDRNN LMVADDKVWR V
Length:971
Mass (Da):107,518
Last modified:March 1, 1992 - v1
Checksum:i43DD4DACAF4456DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53540 Genomic DNA. Translation: CAA37616.1.
U18779 Genomic DNA. Translation: AAB65010.1.
BK006939 Genomic DNA. Translation: DAA07621.1.
PIRiA36376.
RefSeqiNP_010882.1. NM_001178847.1.

Genome annotation databases

EnsemblFungiiYEL032W; YEL032W; YEL032W.
GeneIDi856680.
KEGGisce:YEL032W.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMCM3_YEAST
AccessioniPrimary (citable) accession number: P24279
Secondary accession number(s): D3DLL7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: July 5, 2017
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome V
    Yeast (Saccharomyces cerevisiae) chromosome V: entries and gene names