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Protein

Phosphoglycerate kinase

Gene

pgk

Organism
Bacillus megaterium (strain DSM 319)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk), Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei36SubstrateBy similarity1
Binding sitei118SubstrateBy similarity1
Binding sitei151SubstrateBy similarity1
Binding sitei201ATPBy similarity1
Binding sitei292ATP; via carbonyl oxygenBy similarity1
Binding sitei323ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi350 – 353ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase (EC:2.7.2.3)
Gene namesi
Name:pgk
Ordered Locus Names:BMD_5037
OrganismiBacillus megaterium (strain DSM 319)
Taxonomic identifieri592022 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000002365 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001459031 – 394Phosphoglycerate kinaseAdd BLAST394

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei183PhosphoserineBy similarity1
Modified residuei299PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP24269.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP24269.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni21 – 23Substrate bindingBy similarity3
Regioni59 – 62Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

HOGENOMiHOG000227108.
KOiK00927.
OMAiEVLMVEN.
OrthoDBiPOG091H00YN.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24269-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKKTLKDID VKGKRVFCRV DFNVPMKDGK VTDETRIRAA IPTIQYLVEQ
60 70 80 90 100
GAKVILASHL GRPKGEVVEE LRLNAVAERL QALLGKDVAK ADEAFGEEVK
110 120 130 140 150
KTIDGMSEGD VLVLENVRFY PGEEKNDPEL AKAFAELADV YVNDAFGAAH
160 170 180 190 200
RAHASTEGIA QHIPAVAGFL MEKELDVLSK ALSNPERPFT AIVGGAKVKD
210 220 230 240 250
KIGVIDHLLD KVDNLIIGGG LSYTFIKALG HEVGKSLLEE DKIELAKSFM
260 270 280 290 300
EKAKKNGVNF YMPVDVVVAD DFSNDANIQV VSIEDIPSDW EGLDAGPKTR
310 320 330 340 350
EIYADVIKNS KLVIWNGPMG VFELDAFANG TKAVAEALAE ATDTYSVIGG
360 370 380 390
GDSAAAVEKF NLADKMSHIS TGGGASLEFM EGKELPGVVA LNDK
Length:394
Mass (Da):42,457
Last modified:March 1, 1992 - v1
Checksum:i7A2E6B978FA7008B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54519 Genomic DNA. Translation: CAA38375.1.
M87647 Genomic DNA. Translation: AAA73203.1.
M87648 Genomic DNA. Translation: AAA73206.1.
CP001982 Genomic DNA. Translation: ADF41837.1.
PIRiS13125. KIBSGM.
RefSeqiWP_013059701.1. NC_014103.1.

Genome annotation databases

EnsemblBacteriaiADF41837; ADF41837; BMD_5037.
GeneIDi9120428.
KEGGibmd:BMD_5037.
PATRICi37260261. VBIBacMeg104484_5015.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54519 Genomic DNA. Translation: CAA38375.1.
M87647 Genomic DNA. Translation: AAA73203.1.
M87648 Genomic DNA. Translation: AAA73206.1.
CP001982 Genomic DNA. Translation: ADF41837.1.
PIRiS13125. KIBSGM.
RefSeqiWP_013059701.1. NC_014103.1.

3D structure databases

ProteinModelPortaliP24269.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP24269.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADF41837; ADF41837; BMD_5037.
GeneIDi9120428.
KEGGibmd:BMD_5037.
PATRICi37260261. VBIBacMeg104484_5015.

Phylogenomic databases

HOGENOMiHOG000227108.
KOiK00927.
OMAiEVLMVEN.
OrthoDBiPOG091H00YN.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGK_BACMD
AccessioniPrimary (citable) accession number: P24269
Secondary accession number(s): D5DNB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.