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Protein

Phosphoglycerate kinase

Gene

pgk

Organism
Bacillus megaterium (strain DSM 319)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361SubstrateBy similarity
Binding sitei118 – 1181SubstrateBy similarity
Binding sitei151 – 1511SubstrateBy similarity
Binding sitei201 – 2011ATPBy similarity
Binding sitei292 – 2921ATP; via carbonyl oxygenBy similarity
Binding sitei323 – 3231ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi350 – 3534ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphoglycerate kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBMEG592022:GIVX-5037-MONOMER.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase (EC:2.7.2.3)
Gene namesi
Name:pgk
Ordered Locus Names:BMD_5037
OrganismiBacillus megaterium (strain DSM 319)
Taxonomic identifieri592022 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000002365 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 394394Phosphoglycerate kinasePRO_0000145903Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei183 – 1831PhosphoserineBy similarity
Modified residuei299 – 2991PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP24269.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP24269.
SMRiP24269. Positions 1-394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni21 – 233Substrate bindingBy similarity
Regioni59 – 624Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Phylogenomic databases

HOGENOMiHOG000227108.
KOiK00927.
OMAiWEALDIG.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24269-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKKTLKDID VKGKRVFCRV DFNVPMKDGK VTDETRIRAA IPTIQYLVEQ
60 70 80 90 100
GAKVILASHL GRPKGEVVEE LRLNAVAERL QALLGKDVAK ADEAFGEEVK
110 120 130 140 150
KTIDGMSEGD VLVLENVRFY PGEEKNDPEL AKAFAELADV YVNDAFGAAH
160 170 180 190 200
RAHASTEGIA QHIPAVAGFL MEKELDVLSK ALSNPERPFT AIVGGAKVKD
210 220 230 240 250
KIGVIDHLLD KVDNLIIGGG LSYTFIKALG HEVGKSLLEE DKIELAKSFM
260 270 280 290 300
EKAKKNGVNF YMPVDVVVAD DFSNDANIQV VSIEDIPSDW EGLDAGPKTR
310 320 330 340 350
EIYADVIKNS KLVIWNGPMG VFELDAFANG TKAVAEALAE ATDTYSVIGG
360 370 380 390
GDSAAAVEKF NLADKMSHIS TGGGASLEFM EGKELPGVVA LNDK
Length:394
Mass (Da):42,457
Last modified:March 1, 1992 - v1
Checksum:i7A2E6B978FA7008B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54519 Genomic DNA. Translation: CAA38375.1.
M87647 Genomic DNA. Translation: AAA73203.1.
M87648 Genomic DNA. Translation: AAA73206.1.
CP001982 Genomic DNA. Translation: ADF41837.1.
PIRiS13125. KIBSGM.
RefSeqiYP_003600187.1. NC_014103.1.

Genome annotation databases

EnsemblBacteriaiADF41837; ADF41837; BMD_5037.
KEGGibmd:BMD_5037.
PATRICi37260261. VBIBacMeg104484_5015.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54519 Genomic DNA. Translation: CAA38375.1.
M87647 Genomic DNA. Translation: AAA73203.1.
M87648 Genomic DNA. Translation: AAA73206.1.
CP001982 Genomic DNA. Translation: ADF41837.1.
PIRiS13125. KIBSGM.
RefSeqiYP_003600187.1. NC_014103.1.

3D structure databases

ProteinModelPortaliP24269.
SMRiP24269. Positions 1-394.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP24269.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADF41837; ADF41837; BMD_5037.
KEGGibmd:BMD_5037.
PATRICi37260261. VBIBacMeg104484_5015.

Phylogenomic databases

HOGENOMiHOG000227108.
KOiK00927.
OMAiWEALDIG.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.
BioCyciBMEG592022:GIVX-5037-MONOMER.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the phosphoglycerate kinase gene from Bacillus megaterium."
    Schlaepfer B.S., Branlant C., Branlant G., Zuber H.
    Nucleic Acids Res. 18:6423-6423(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Cloning and sequencing of the genes encoding glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase and triosephosphate isomerase (gap operon) from mesophilic Bacillus megaterium: comparison with corresponding sequences from thermophilic Bacillus stearothermophilus."
    Schlaepfer B.S., Zuber H.
    Gene 122:53-62(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Genome sequences of the industrial vitamin B12-producers B. megaterium QM B1551 and DSM319 reveal new insights into the Bacillus genome evolution and pan-genome structure."
    Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K., Riley D.R., Creasy H.H., Koenig S.S.K., Galens K., Orvis J., Creasy T., Biedendieck R., Braun C., Grayburn S., Jahn D., Ravel J., Vary P.S.
    Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 319.

Entry informationi

Entry nameiPGK_BACMD
AccessioniPrimary (citable) accession number: P24269
Secondary accession number(s): D5DNB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: April 29, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.