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P24269 (PGK_BACMD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase

EC=2.7.2.3
Gene names
Name:pgk
Ordered Locus Names:BMD_5037
OrganismBacillus megaterium (strain DSM 319) [Complete proteome] [HAMAP]
Taxonomic identifier592022 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length394 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145

Subunit structure

Monomer.

Subcellular location

Cytoplasm HAMAP-Rule MF_00145.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 394394Phosphoglycerate kinase HAMAP-Rule MF_00145
PRO_0000145903

Regions

Nucleotide binding350 – 3534ATP By similarity
Region21 – 233Substrate binding By similarity
Region59 – 624Substrate binding By similarity

Sites

Binding site361Substrate By similarity
Binding site1181Substrate By similarity
Binding site1511Substrate By similarity
Binding site2011ATP By similarity
Binding site2921ATP; via carbonyl oxygen By similarity
Binding site3231ATP By similarity

Amino acid modifications

Modified residue1831Phosphoserine By similarity
Modified residue2991Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
P24269 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 7A2E6B978FA7008B

FASTA39442,457
        10         20         30         40         50         60 
MNKKTLKDID VKGKRVFCRV DFNVPMKDGK VTDETRIRAA IPTIQYLVEQ GAKVILASHL 

        70         80         90        100        110        120 
GRPKGEVVEE LRLNAVAERL QALLGKDVAK ADEAFGEEVK KTIDGMSEGD VLVLENVRFY 

       130        140        150        160        170        180 
PGEEKNDPEL AKAFAELADV YVNDAFGAAH RAHASTEGIA QHIPAVAGFL MEKELDVLSK 

       190        200        210        220        230        240 
ALSNPERPFT AIVGGAKVKD KIGVIDHLLD KVDNLIIGGG LSYTFIKALG HEVGKSLLEE 

       250        260        270        280        290        300 
DKIELAKSFM EKAKKNGVNF YMPVDVVVAD DFSNDANIQV VSIEDIPSDW EGLDAGPKTR 

       310        320        330        340        350        360 
EIYADVIKNS KLVIWNGPMG VFELDAFANG TKAVAEALAE ATDTYSVIGG GDSAAAVEKF 

       370        380        390 
NLADKMSHIS TGGGASLEFM EGKELPGVVA LNDK 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the phosphoglycerate kinase gene from Bacillus megaterium."
Schlaepfer B.S., Branlant C., Branlant G., Zuber H.
Nucleic Acids Res. 18:6423-6423(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Cloning and sequencing of the genes encoding glyceraldehyde-3-phosphate dehydrogenase, phosphoglycerate kinase and triosephosphate isomerase (gap operon) from mesophilic Bacillus megaterium: comparison with corresponding sequences from thermophilic Bacillus stearothermophilus."
Schlaepfer B.S., Zuber H.
Gene 122:53-62(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Genome sequences of the industrial vitamin B12-producers B. megaterium QM B1551 and DSM319 reveal new insights into the Bacillus genome evolution and pan-genome structure."
Eppinger M., Bunk B., Johns M.A., Edirisinghe J.N., Kutumbaka K.K., Riley D.R., Creasy H.H., Koenig S.S.K., Galens K., Orvis J., Creasy T., Biedendieck R., Braun C., Grayburn S., Jahn D., Ravel J., Vary P.S.
Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 319.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54519 Genomic DNA. Translation: CAA38375.1.
M87647 Genomic DNA. Translation: AAA73203.1.
M87648 Genomic DNA. Translation: AAA73206.1.
CP001982 Genomic DNA. Translation: ADF41837.1.
PIRKIBSGM. S13125.
RefSeqYP_003600187.1. NC_014103.1.

3D structure databases

ProteinModelPortalP24269.
SMRP24269. Positions 1-394.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP24269.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADF41837; ADF41837; BMD_5037.
GeneID9120428.
KEGGbmd:BMD_5037.
PATRIC37260261. VBIBacMeg104484_5015.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000227108.
KOK00927.
OMAFGLADKM.
ProtClustDBCLSK2840960.

Enzyme and pathway databases

BioCycBMEG592022:GIVX-5037-MONOMER.
UniPathwayUPA00109; UER00185.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGK_BACMD
AccessionPrimary (citable) accession number: P24269
Secondary accession number(s): D5DNB0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: February 19, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways