P24260 (METK2_DIACA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-adenosylmethionine synthase 2
Short name=AdoMet synthase 2
EC=2.5.1.6

Alternative name(s):
Methionine adenosyltransferase 2
Short name=MAT 2

Gene names

Name:

SAM2

Organism

Dianthus caryophyllus (Carnation) (Clove pink)

Taxonomic identifier

3570 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Caryophyllaceae › Dianthus

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AdoMet synthase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Gene Ontology (GO)
Biological process	S-adenosylmethionine biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine adenosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	S-adenosylmethionine synthase 2		PRO_0000174462
Regions
	Nucleotide binding	123 – 128	6	ATP Potential
	Nucleotide binding	271 – 278	8	ATP Potential
Sites
	Metal binding	21	1	Magnesium By similarity
	Metal binding	47	1	Potassium By similarity
	Metal binding	275	1	Potassium By similarity
	Metal binding	283	1	Magnesium By similarity
	Binding site	151	1	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P24260 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 959024A69865E2D9	FASTA	396	43,189
	10 20 30 40 50 60 MAAAADTFLF TSESVNEGHP DKLCDQISDA VLDACLAQDA ESKVACETCT KTNLVMVFGE 70 80 90 100 110 120 ITTKANVDYE KIVADTCREI GFVSPDVGLD ADNCKVLVYI EQQSPDIAQG VHGHLTKRPE 130 140 150 160 170 180 DIGAGDQGHM FGYATDETPE LMPLSHVLAT KLGARLTEVR KNGTCAWLRP DGKTQVTVEY 190 200 210 220 230 240 YNENGAMVPI RVHTVLISTQ HDETVTNDEI AADLKEHVIK PVIPEKYLDE NTIFHLNPSG 250 260 270 280 290 300 RFVIGGPHGD AGLTGRKIII DTYGGWGAHG GGAFSRKDPT KVDRSGAYIA RQAAKSIVAS 310 320 330 340 350 360 GLARRCIVQI SYAIGVPEPL SVFVDTYGTG KIHDREILKI VKENFDFRPG MIAIALDLKK 370 380 390 GGNRYLKTAA YGHFGREDPD FTWEAAKTLK WEKPQA

References

[1]	"Cloning and nucleotide sequence of a S-adenosylmethionine synthetase cDNA clone from carnation." Larsen P.B., Woodson W.R. Plant Physiol. 96:997-999(1991) [PubMed: 16668288] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M61882 mRNA. Translation: AAA33274.1.
PIR	T10710.
3D structure databases
ProteinModelPortal	P24260.
SMR	P24260. Positions 7-391.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR022631. ADOMET_SYNTHASE_CS. IPR022630. S-AdoMet_synt_C. IPR022629. S-AdoMet_synt_central. IPR022628. S-AdoMet_synt_N. IPR002133. S-AdoMet_synthetase. IPR022636. S-AdoMet_synthetase_sfam. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
SUPFAM	SSF55973. S-AdoMet_synt. 3 hits.
TIGRFAMs	TIGR01034. MetK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHASE_1. 1 hit. PS00377. ADOMET_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METK2_DIACA

Accession

Primary (citable) accession number: P24260

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	June 28, 2011
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program