Reviewed, UniProtKB/Swiss-Prot P24258 (CAH2_CHLRE)

Names and origin

Protein names

Recommended name:
    Carbonic anhydrase 2
    EC=4.2.1.1
Alternative name(s):
    Carbonate dehydratase 2
      Short name=CA2
Cleaved into the following 2 chains:
    1- Recommended name:
            Carbonic anhydrase 2 large chain
    2- Recommended name:
            Carbonic anhydrase 2 small chain

Gene names

Name:

CAH2

Organism

Chlamydomonas reinhardtii

Taxonomic identifier

3055 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reversible hydration of carbon dioxide.
Catalytic activity	H2CO3 = CO2 + H2O.
Cofactor	Zinc.
Subunit structure	Tetramer of two large and two small subunits linked by two disulfide bonds.
Subcellular location	Periplasm.
Induction	Expressed under high-CO2 condition. Ref.2
Sequence similarities	Belongs to the alpha-carbonic anhydrase family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Lyase
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carbonate dehydratase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Ref.3 Ref.4
	Chain	21 – 380	360	Carbonic anhydrase 2		PRO_0000004260
	Chain	21 – ?343	323	Carbonic anhydrase 2 large chain		PRO_0000004261
	Chain	344 – 380	37	Carbonic anhydrase 2 small chain		PRO_0000004262
Regions
	Compositional bias	310 – 324	15	His-rich
Sites
	Metal binding	163	1	Zinc; catalytic By similarity
	Metal binding	165	1	Zinc; catalytic By similarity
	Metal binding	182	1	Zinc; catalytic By similarity
Amino acid modifications
	Glycosylation	101	1	N-linked (GlcNAc...) Potential
	Glycosylation	135	1	N-linked (GlcNAc...) Potential
	Glycosylation	297	1	N-linked (GlcNAc...) Potential
	Disulfide bond	21		Interchain By similarity
	Disulfide bond	61 ↔ 264		By similarity
	Disulfide bond	194 ↔ 198		By similarity
	Disulfide bond	296 ↔ 354		Interchain (between large and small chains) By similarity
Experimental info
	Sequence conflict	220 – 225	6	Missing in CAA38360. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P24258-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 5ABC3823A1DD7862	FASTA	380	42,172
	10 20 30 40 50 60 MARTGALLLA ALALAGCAQA CIYKFGTSPD SKATHTGDHW DHSLNGENWE GKDGAGNPWV 70 80 90 100 110 120 CKTGRKQSPI NVPQYHVLDG KGSKIATGLQ TQWSYPDLMS NGSSVQVINN GHTIQVQWTY 130 140 150 160 170 180 DYAGHATIAI PAMRNQSNRI VDVLEMRPND ASDRVTAVPT QFHFHSTSEH LLAGKIFPLE 190 200 210 220 230 240 LHIVHKVTDK LEACKGGCFS VTGILFQLDN GPDNELLEPI FANMPTREGT FTNLPAGTTI 250 260 270 280 290 300 KLGELLPSDR DYVTYEGSLT TPPCSEGLLW HVMTQPQRIS FGQWNRYRLA VGEKECNSTE 310 320 330 340 350 360 TDAAHADAGH HHHHHRRLLH NHAHLEEVPA ATSEPKHYFR RVMEETENPD AYTCTTVAFG 370 380 QNFRNAQYAN GRTIKLARYE

References

[1]	"Nucleotide sequences of two genes CAH1 and CAH2 which encode carbonic anhydrase polypeptides in Chlamydomonas reinhardtii." Fukuzawa H., Fujiwara S., Tachiki A., Miyachi S. Nucleic Acids Res. 18:6441-6442(1990) [PubMed: 2243800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: IAM C-9.
[2]	"Structure and differential expression of two genes encoding carbonic anhydrase in Chlamydomonas reinhardtii." Fujiwara S., Fukuzawa H., Tachiki A., Miyachi S. Proc. Natl. Acad. Sci. U.S.A. 87:9779-9783(1990) [PubMed: 2124702] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
[3]	"Partial characterization of a new isoenzyme of carbonic anhydrase isolated from Chlamydomonas reinhardtii." Rawat M., Moroney J.V. J. Biol. Chem. 266:9719-9723(1991) [PubMed: 1903396] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-41 AND 344-363.
[4]	"Characterization of carbonic anhydrase isozyme CA2, which is the CAH2 gene product, in Chlamydomonas reinhardtii." Tachiki A., Fukuzawa H., Miyachi S. Biosci. Biotechnol. Biochem. 56:794-798(1992) [PubMed: 1368343] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-41 AND 344-364, CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54488 Genomic DNA. Translation: CAA38360.1.
PIR	S14188.
RefSeq	XP_001692290.1.
UniGene	Cre.16115
3D structure databases
SMR	P24258. Positions 38-293.
ModBase	Search...
Proteomic databases
PRIDE	P24258.
Genome annotation databases
GeneID	5717780.
KEGG	cre:CHLREDRAFT_128726.
Enzyme and pathway databases
BRENDA	4.2.1.1. 144.
Family and domain databases
InterPro	IPR001148. Carbonic_anhydrase_a-class_cat. IPR018338. Carbonic_anhydrase_a-class_CS. IPR018340. Carbonic_anhydrase_CAH1-like. [Graphical view]
Gene3D	G3DSA:3.10.200.10. Euk_COanhd. 1 hit.
PANTHER	PTHR18952:SF2. Carbonic_anhydrase_CAH1-like. 1 hit. PTHR18952. Euk_COanhd. 1 hit.
Pfam	PF00194. Carb_anhydrase. 1 hit. [Graphical view]
PROSITE	PS00162. ALPHA_CA_1. 1 hit. PS51144. ALPHA_CA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAH2_CHLRE

Accession

Primary (citable) accession number: P24258

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	January 19, 2010
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)