P24257 (WNT1_DANRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-1

Gene names

Name:	wnt1
Synonyms:	int-1, wnt-1

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. Involved in neurogenesis. Performs a partially redundant function with wnt10b in the formation of the midbrain-hindbrain boundary (MHB) organizer. In the hindbrain, mediates lateral inhibition of boundary cell specification, probably via up-regulation of proneural and Delta gene expression in non-boundary cells; localized expression of wnt1 in boundary cells is maintained via rfng-mediated modulation of Notch activity. Ref.2 Ref.3 Ref.4
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	In the embryo, expressed in the developing central nervous system (CNS), with an expression pattern very similar to that of wnt10b. During somitogenesis, expressed strongly in hindbrain boundary and roof plate cells. Ref.1 Ref.2 Ref.4
Developmental stage	First detected at around 8 hours post-fertilization (hpf) within the prospective midbrain-hindbrain boundary (MHB). By 10 hpf, expressed in two stripes that converge at the dorsal midline. During somitogenesis, this expression domain extends to the prospective epiphysis and the dorsal midline of the hindbrain; unlike wnt10b, wnt1 is not detected in the prospective cerebellum. The hindbrain domain bifurcates along the midline to from two dorsolateral columns with transverse bands of up-regulation at rhombomere boundaries; these are refined into stripes which are maintained at least until at least 48 hpf. At 30 hpf, expressed in the epiphysis, the dorsal midline of the optic tectum, the anterior half of the MHB constriction and in the hindbrain walls. Not detected in adults. Ref.1 Ref.2 Ref.3
Miscellaneous	Morpholino knockdown results in broadening of hindbrain boundary marker expression.
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	T cell differentiation in thymus Inferred from Biological aspect of Ancestor. Source: RefGenome Wnt receptor signaling pathway, calcium modulating pathway Inferred from Biological aspect of Ancestor. Source: RefGenome axis specification Inferred from Biological aspect of Ancestor. Source: RefGenome brain segmentation Inferred from mutant phenotype Ref.4. Source: ZFIN canonical Wnt receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome cerebellum morphogenesis Inferred from mutant phenotype. Source: ZFIN diencephalon development Inferred from Biological aspect of Ancestor. Source: RefGenome embryonic pattern specification Inferred from Biological aspect of Ancestor. Source: RefGenome forebrain anterior/posterior pattern specification Inferred from Biological aspect of Ancestor. Source: RefGenome fourth ventricle development Inferred from mutant phenotype. Source: ZFIN hemopoietic stem cell proliferation Inferred from Biological aspect of Ancestor. Source: RefGenome hepatocyte differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome inner ear morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome mesoderm morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome midbrain development Inferred from Biological aspect of Ancestor. Source: RefGenome midbrain-hindbrain boundary maturation during brain development Inferred from Biological aspect of Ancestor. Source: RefGenome negative regulation of fat cell differentiation Inferred from Biological aspect of Ancestor. Source: RefGenome neuron fate determination Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of cell proliferation Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular component	early endosome Inferred from Biological aspect of Ancestor. Source: RefGenome extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome late endosome Inferred from Biological aspect of Ancestor. Source: RefGenome membrane raft Inferred from Biological aspect of Ancestor. Source: RefGenome plasma membrane Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	frizzled-2 binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 370	351	Protein Wnt-1		PRO_0000041408
Regions
	Compositional bias	325 – 360	36	Cys-rich
Amino acid modifications
	Glycosylation	28	1	N-linked (GlcNAc...) Potential
	Glycosylation	316	1	N-linked (GlcNAc...) Potential
	Glycosylation	359	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P24257 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: F2AB0A82DB031D3E	FASTA	370	41,011
	10 20 30 40 50 60 MRVLALLLAV KAACVLLVSS LTGTGAVNNS GRWWGIVNVA SSGNLLTNSK NVQLVLDPSL 70 80 90 100 110 120 ALLSRRQRKL IRQNPGILHA IAAGLHTAIK ECKWQFRNRR WNCPTTHSPN VFGKIVNRGC 130 140 150 160 170 180 RETAFVFAIT SAGVTHAVAR SCSEGAIESC TCDYRRRGPG GPDWHWGGCS DNVEFGRMFG 190 200 210 220 230 240 REFVDSSERG RDLRYLTNLH NNEAGRMTVA SEMQQECKCH GMSGSCTVRT CWMRLPSFRL 250 260 270 280 290 300 VGDYLKDRFD GASRVVYANK GSNRASHRAD PRHLEPENPA HKLPSSRDLV YFEKSPNFCS 310 320 330 340 350 360 YNGKTGTHGT SGRTCNSSSP ALDGCELLCC GRGYKTRMEQ VTERCHCTFH WCCHVSCLNC 370 TSTQTVHQCL

References

[1]	"Genomic structure and restricted neural expression of the zebrafish wnt-1 (int-1) gene." Molven A., Njolstad P.R., Fjose A. EMBO J. 10:799-807(1991) [PubMed: 2009859] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	"Wnt1 and wnt10b function redundantly at the zebrafish midbrain-hindbrain boundary." Lekven A.C., Buckles G.R., Kostakis N., Moon R.T. Dev. Biol. 254:172-187(2003) [PubMed: 12591239] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[3]	"Rhombomere boundaries are Wnt signaling centers that regulate metameric patterning in the zebrafish hindbrain." Riley B.B., Chiang M.-Y., Storch E.M., Heck R., Buckles G.R., Lekven A.C. Dev. Dyn. 231:278-291(2004) [PubMed: 15366005] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[4]	"Wnt1 regulates neurogenesis and mediates lateral inhibition of boundary cell specification in the zebrafish hindbrain." Amoyel M., Cheng Y.-C., Jiang Y.-J., Wilkinson D.G. Development 132:775-785(2005) [PubMed: 15659486] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X58880 , X58881, X58882, X58883 Genomic DNA. Translation: CAA41687.1.
IPI	IPI00484391.
PIR	S15013.
RefSeq	NP_001188327.1. NM_001201398.1.
UniGene	Dr.85371.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P24257.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000077931; ENSDARP00000072397; ENSDARG00000055554.
GeneID	30128.
KEGG	dre:30128.
Organism-specific databases
CTD	7471.
ZFIN	ZDB-GENE-980526-526. wnt1.
Phylogenomic databases
eggNOG	fiNOG12648.
GeneTree	ENSGT00600000084119.
HOGENOM	HBG446188.
HOVERGEN	HBG001595.
InParanoid	P24257.
OMA	MWWGIAK.
OrthoDB	EOG4R23V2.
Gene expression databases
ArrayExpress	P24257.
Bgee	P24257.
Family and domain databases
InterPro	IPR005817. Wnt. IPR009139. Wnt1. IPR018161. Wnt_grthfactor_CS. [Graphical view]
PANTHER	PTHR12027. Wnt. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01841. WNT1PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

WNT1_DANRE

Accession

Primary (citable) accession number: P24257

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	October 19, 2011
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program