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Protein

Sigma factor-binding protein Crl

Gene

crl

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the sigma-S subunit of RNA polymerase, activating expression of sigma-S-regulated genes, such as the csgBAC operon encoding the subunits of curli proteins. Stimulates RNA polymerase holoenzyme formation and may bind to several other sigma factors, such as sigma-70 (rpoD) and sigma-32 (rpoH).2 Publications

GO - Biological processi

  • cellular protein complex assembly Source: EcoCyc
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG11092-MONOMER.
ECOL316407:JW0230-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sigma factor-binding protein Crl
Alternative name(s):
Curlin genes transcriptional activatory protein
Gene namesi
Name:crl
Ordered Locus Names:b0240, JW0230
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11092. crl.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 133132Sigma factor-binding protein CrlPRO_0000079355Add
BLAST

Proteomic databases

EPDiP24251.
PaxDbiP24251.
PRIDEiP24251.

Expressioni

Inductioni

Induced at stationary phase, and by low temperatures.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
pspAP0AFM61EBI-1114986,EBI-1123459
tatEP0A8431EBI-1114986,EBI-1112947

Protein-protein interaction databases

BioGridi4260821. 12 interactions.
IntActiP24251. 11 interactions.
STRINGi511145.b0240.

Structurei

3D structure databases

ProteinModelPortaliP24251.
SMRiP24251. Positions 7-131.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni99 – 12224Essential for activityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili90 – 11627Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the Crl family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4108UVK. Bacteria.
ENOG4111IQF. LUCA.
HOGENOMiHOG000282284.
KOiK11926.
OMAiGWWLNLE.
OrthoDBiEOG64FKG6.

Family and domain databases

HAMAPiMF_01178. Crl.
InterProiIPR009986. Tscrpt_reg_Crl.
[Graphical view]
PfamiPF07417. Crl. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24251-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLPSGHPKS RLIKKFTALG PYIREGKCKD NRFFFDCLAV CVNVKPAPEV
60 70 80 90 100
REFWGWWMEL EAQESRFTYS YQFGLFDKAG DWKSVPVKDT EVVERLEHTL
110 120 130
REFHEKLREL LTTLNLKLEP ADDFRDEPVK LTA
Length:133
Mass (Da):15,655
Last modified:January 23, 2007 - v5
Checksum:iEA66172EF19586AA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81P → L in CAA23606 (PubMed:6341601).Curated
Sequence conflicti13 – 131I → N in CAA23606 (PubMed:6341601).Curated
Sequence conflicti29 – 291K → E (PubMed:2649795).Curated
Sequence conflicti29 – 291K → E in CAA47646 (PubMed:1357528).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00316 Genomic DNA. Translation: CAA23606.1.
X67207 Genomic DNA. Translation: CAA47646.1.
U70214 Genomic DNA. Translation: AAB08660.1.
U00096 Genomic DNA. No translation available.
AP009048 Genomic DNA. Translation: BAA77909.2.
M13422 Genomic DNA. Translation: AAA23930.1.
PIRiA64749.
RefSeqiWP_000174689.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiBAA77909; BAA77909; BAA77909.
KEGGiecj:JW0230.
PATRICi32115595. VBIEscCol129921_0242.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00316 Genomic DNA. Translation: CAA23606.1.
X67207 Genomic DNA. Translation: CAA47646.1.
U70214 Genomic DNA. Translation: AAB08660.1.
U00096 Genomic DNA. No translation available.
AP009048 Genomic DNA. Translation: BAA77909.2.
M13422 Genomic DNA. Translation: AAA23930.1.
PIRiA64749.
RefSeqiWP_000174689.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP24251.
SMRiP24251. Positions 7-131.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260821. 12 interactions.
IntActiP24251. 11 interactions.
STRINGi511145.b0240.

Proteomic databases

EPDiP24251.
PaxDbiP24251.
PRIDEiP24251.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA77909; BAA77909; BAA77909.
KEGGiecj:JW0230.
PATRICi32115595. VBIEscCol129921_0242.

Organism-specific databases

EchoBASEiEB1084.
EcoGeneiEG11092. crl.

Phylogenomic databases

eggNOGiENOG4108UVK. Bacteria.
ENOG4111IQF. LUCA.
HOGENOMiHOG000282284.
KOiK11926.
OMAiGWWLNLE.
OrthoDBiEOG64FKG6.

Enzyme and pathway databases

BioCyciEcoCyc:EG11092-MONOMER.
ECOL316407:JW0230-MONOMER.

Miscellaneous databases

PROiP24251.

Family and domain databases

HAMAPiMF_01178. Crl.
InterProiIPR009986. Tscrpt_reg_Crl.
[Graphical view]
PfamiPF07417. Crl. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of phoE, the structural gene for the phosphate limitation inducible outer membrane pore protein of Escherichia coli K12."
    Overbeeke N., Bergmans H., van Mansfeld F., Lugtenberg B.
    J. Mol. Biol. 163:513-532(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Fibronectin binding mediated by a novel class of surface organelles on Escherichia coli."
    Olsen A., Jonsson A., Normark S.
    Nature 338:652-655(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-6, CHARACTERIZATION.
    Strain: AO12.
  3. "The Crl protein activates cryptic genes for curli formation and fibronectin binding in Escherichia coli HB101."
    Arnqvist A., Olsen A., Pfeifer J., Russell D.G., Normark S.
    Mol. Microbiol. 6:2443-2452(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: AO12.
  4. "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region."
    Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.
    Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Newly identified genetic variations in common Escherichia coli MG1655 stock cultures."
    Freddolino P.L., Amini S., Tavazoie S.
    J. Bacteriol. 194:303-306(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
    Strain: K12 / MG1655 / ATCC 700926.
  8. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 28.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  9. "Structural and functional organization of the gpt gene region of Escherichia coli."
    Nueesch J., Schuemperli D.
    Gene 32:243-249(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
  10. "The rcsA gene of Escherichia coli 09:K30:H12 is involved in the expression of the serotype-specific group I K (capsular) antigen."
    Keenleyside W.J., Jayaratne P., MacLachlan P.R., Whitfield C.
    J. Bacteriol. 174:8-16(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
    Strain: O9:K30:H12.
  11. "Crl stimulates RpoS activity during stationary phase."
    Pratt L.A., Silhavy T.J.
    Mol. Microbiol. 29:1225-1236(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACTIVATION OF RPOS ACTIVITY.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  12. "Crl, a low temperature-induced protein in Escherichia coli that binds directly to the stationary phase sigma subunit of RNA polymerase."
    Bougdour A., Lelong C., Geiselmann J.
    J. Biol. Chem. 279:19540-19550(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: BINDING TO SIGMA-SUBUNIT OF RNA POLYMERASE.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  13. "Crl facilitates RNA polymerase holoenzyme formation."
    Gaal T., Mandel M.J., Silhavy T.J., Gourse R.L.
    J. Bacteriol. 188:7966-7970(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN RNA POLYMERASE HOLOENZYME FORMATION.

Entry informationi

Entry nameiCRL_ECOLI
AccessioniPrimary (citable) accession number: P24251
Secondary accession number(s): P77629, Q47242, Q47585
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: March 16, 2016
This is version 119 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

A pseudogene in strain MG1655 / ATCC 700926, but not its parent MG1655 / ATCC 47076. In ATCC 700926 IS1I has recently inserted into the gene (PubMed:22081388).1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.