P24251 (CRL_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sigma factor-binding protein Crl

Alternative name(s):
Curlin genes transcriptional activatory protein

Gene names

Name:	crl
Ordered Locus Names:	b0240, JW0230

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	133 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to the sigma-S subunit of RNA polymerase, activating expression of sigma-S-regulated genes, such as the csgBAC operon encoding the subunits of curli proteins. Stimulates RNA polymerase holoenzyme formation and may bind to several other sigma factors, such as sigma-70 and sigma-32. Ref.3 Ref.12
Subcellular location	Cytoplasm HAMAP-Rule MF_01178.
Induction	Induced at stationary phase, and by low temperatures. HAMAP-Rule MF_01178
Sequence similarities	Belongs to the Crl family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Domain	Coiled coil
Molecular function	Activator
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	positive regulation of transcription, DNA-dependent Inferred from direct assay PubMed 17332743. Source: EcoCyc transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sigma factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
pspA	P0AFM6	1	EBI-1114986,EBI-1123459
tatE	P0A843	1	EBI-1114986,EBI-1112947

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 133	132	Sigma factor-binding protein Crl HAMAP-Rule MF_01178		PRO_0000079355
Regions
	Region	99 – 122	24	Essential for activity HAMAP-Rule MF_01178
	Coiled coil	90 – 116	27	Potential
Experimental info
	Sequence conflict	8	1	P → L in CAA23606. Ref.1
	Sequence conflict	13	1	I → N in CAA23606. Ref.1
	Sequence conflict	29	1	K → E Ref.2
	Sequence conflict	29	1	K → E Ref.3
	Sequence conflict	29	1	K → E Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P24251 [UniParc]. Last modified January 23, 2007. Version 5. Checksum: EA66172EF19586AA	FASTA	133	15,655
	10 20 30 40 50 60 MTLPSGHPKS RLIKKFTALG PYIREGKCKD NRFFFDCLAV CVNVKPAPEV REFWGWWMEL 70 80 90 100 110 120 EAQESRFTYS YQFGLFDKAG DWKSVPVKDT EVVERLEHTL REFHEKLREL LTTLNLKLEP 130 ADDFRDEPVK LTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of phoE, the structural gene for the phosphate limitation inducible outer membrane pore protein of Escherichia coli K12." Overbeeke N., Bergmans H., van Mansfeld F., Lugtenberg B. J. Mol. Biol. 163:513-532(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Fibronectin binding mediated by a novel class of surface organelles on Escherichia coli." Olsen A., Jonsson A., Normark S. Nature 338:652-655(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-6, CHARACTERIZATION. Strain: AO12.
[3]	"The Crl protein activates cryptic genes for curli formation and fibronectin binding in Escherichia coli HB101." Arnqvist A., Olsen A., Pfeifer J., Russell D.G., Normark S. Mol. Microbiol. 6:2443-2452(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: AO12.
[4]	"Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region." Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K. Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[7]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 28. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[8]	"Structural and functional organization of the gpt gene region of Escherichia coli." Nueesch J., Schuemperli D. Gene 32:243-249(1984) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32.
[9]	"The rcsA gene of Escherichia coli 09:K30:H12 is involved in the expression of the serotype-specific group I K (capsular) antigen." Keenleyside W.J., Jayaratne P., MacLachlan P.R., Whitfield C. J. Bacteriol. 174:8-16(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-32. Strain: O9:K30:H12.
[10]	"Crl stimulates RpoS activity during stationary phase." Pratt L.A., Silhavy T.J. Mol. Microbiol. 29:1225-1236(1998) [PubMed] [Europe PMC] [Abstract] Cited for: ACTIVATION OF RPOS ACTIVITY. Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[11]	"Crl, a low temperature-induced protein in Escherichia coli that binds directly to the stationary phase sigma subunit of RNA polymerase." Bougdour A., Lelong C., Geiselmann J. J. Biol. Chem. 279:19540-19550(2004) [PubMed] [Europe PMC] [Abstract] Cited for: BINDING TO SIGMA-SUBUNIT OF RNA POLYMERASE. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[12]	"Crl facilitates RNA polymerase holoenzyme formation." Gaal T., Mandel M.J., Silhavy T.J., Gourse R.L. J. Bacteriol. 188:7966-7970(2006) [PubMed] [Europe PMC] [Abstract] Cited for: ROLE IN RNA POLYMERASE HOLOENZYME FORMATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	V00316 Genomic DNA. Translation: CAA23606.1. X67207 Genomic DNA. Translation: CAA47646.1. U70214 Genomic DNA. Translation: AAB08660.1. U00096 Genomic DNA. Translation: AAC73344.1. AP009048 Genomic DNA. Translation: BAA77909.2. M13422 Genomic DNA. Translation: AAA23930.1.
PIR	A64749.
RefSeq	NP_414775.1. NC_000913.2. YP_488535.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P24251.
SMR	P24251. Positions 7-131.
ModBase	Search...
Protein-protein interaction databases
IntAct	P24251. 10 interactions.
STRING	511145.b0240.
Proteomic databases
PaxDb	P24251.
PRIDE	P24251.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73344; AAC73344; b0240. BAA77909; BAA77909; BAA77909.
GeneID	12931827. 945077.
KEGG	ecj:Y75_p0231. eco:b0240.
PATRIC	32115595. VBIEscCol129921_0242.
Organism-specific databases
EchoBASE	EB1084.
EcoGene	EG11092. crl.
Phylogenomic databases
eggNOG	NOG06255.
HOGENOM	HOG000282284.
KO	K11926.
OMA	DRFFFDC.
ProtClustDB	PRK10984.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11092-MONOMER. ECOL316407:JW0230-MONOMER.
Gene expression databases
Genevestigator	P24251.
Family and domain databases
HAMAP	MF_01178. Crl.
InterPro	IPR009986. Tscrpt_reg_Crl. [Graphical view]
Pfam	PF07417. Crl. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CRL_ECOLI

Accession

Primary (citable) accession number: P24251
Secondary accession number(s): P77629, Q47242, Q47585

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 101 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program