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Protein

Sigma factor-binding protein Crl

Gene

crl

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Binds to the sigma-S subunit of RNA polymerase, activating expression of sigma-S-regulated genes, such as the csgBAC operon encoding the subunits of curli proteins. Stimulates RNA polymerase holoenzyme formation and may bind to several other sigma factors, such as sigma-70 (rpoD) and sigma-32 (rpoH).2 Publications

GO - Biological processi

  • cellular protein complex assembly Source: EcoCyc
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG11092-MONOMER.
ECOL316407:JW0230-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sigma factor-binding protein Crl
Alternative name(s):
Curlin genes transcriptional activatory protein
Gene namesi
Name:crl
Ordered Locus Names:b0240, JW0230
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11092. crl.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000793552 – 133Sigma factor-binding protein CrlAdd BLAST132

Proteomic databases

EPDiP24251.
PaxDbiP24251.

Expressioni

Inductioni

Induced at stationary phase, and by low temperatures.

Interactioni

Protein-protein interaction databases

BioGridi4260821. 12 interactors.
IntActiP24251. 11 interactors.
STRINGi511145.b0240.

Structurei

3D structure databases

ProteinModelPortaliP24251.
SMRiP24251.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni99 – 122Essential for activityAdd BLAST24

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili90 – 116Sequence analysisAdd BLAST27

Sequence similaritiesi

Belongs to the Crl family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4108UVK. Bacteria.
ENOG4111IQF. LUCA.
HOGENOMiHOG000282284.
KOiK11926.
OMAiGWWLNLE.

Family and domain databases

HAMAPiMF_01178. Crl. 1 hit.
InterProiIPR009986. Tscrpt_reg_Crl.
[Graphical view]
PfamiPF07417. Crl. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24251-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLPSGHPKS RLIKKFTALG PYIREGKCKD NRFFFDCLAV CVNVKPAPEV
60 70 80 90 100
REFWGWWMEL EAQESRFTYS YQFGLFDKAG DWKSVPVKDT EVVERLEHTL
110 120 130
REFHEKLREL LTTLNLKLEP ADDFRDEPVK LTA
Length:133
Mass (Da):15,655
Last modified:January 23, 2007 - v5
Checksum:iEA66172EF19586AA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8P → L in CAA23606 (PubMed:6341601).Curated1
Sequence conflicti13I → N in CAA23606 (PubMed:6341601).Curated1
Sequence conflicti29K → E (PubMed:2649795).Curated1
Sequence conflicti29K → E in CAA47646 (PubMed:1357528).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00316 Genomic DNA. Translation: CAA23606.1.
X67207 Genomic DNA. Translation: CAA47646.1.
U70214 Genomic DNA. Translation: AAB08660.1.
U00096 Genomic DNA. No translation available.
AP009048 Genomic DNA. Translation: BAA77909.2.
M13422 Genomic DNA. Translation: AAA23930.1.
PIRiA64749.
RefSeqiWP_000174689.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiBAA77909; BAA77909; BAA77909.
KEGGiecj:JW0230.
PATRICi32115595. VBIEscCol129921_0242.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00316 Genomic DNA. Translation: CAA23606.1.
X67207 Genomic DNA. Translation: CAA47646.1.
U70214 Genomic DNA. Translation: AAB08660.1.
U00096 Genomic DNA. No translation available.
AP009048 Genomic DNA. Translation: BAA77909.2.
M13422 Genomic DNA. Translation: AAA23930.1.
PIRiA64749.
RefSeqiWP_000174689.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP24251.
SMRiP24251.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260821. 12 interactors.
IntActiP24251. 11 interactors.
STRINGi511145.b0240.

Proteomic databases

EPDiP24251.
PaxDbiP24251.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAA77909; BAA77909; BAA77909.
KEGGiecj:JW0230.
PATRICi32115595. VBIEscCol129921_0242.

Organism-specific databases

EchoBASEiEB1084.
EcoGeneiEG11092. crl.

Phylogenomic databases

eggNOGiENOG4108UVK. Bacteria.
ENOG4111IQF. LUCA.
HOGENOMiHOG000282284.
KOiK11926.
OMAiGWWLNLE.

Enzyme and pathway databases

BioCyciEcoCyc:EG11092-MONOMER.
ECOL316407:JW0230-MONOMER.

Miscellaneous databases

PROiP24251.

Family and domain databases

HAMAPiMF_01178. Crl. 1 hit.
InterProiIPR009986. Tscrpt_reg_Crl.
[Graphical view]
PfamiPF07417. Crl. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCRL_ECOLI
AccessioniPrimary (citable) accession number: P24251
Secondary accession number(s): P77629, Q47242, Q47585
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 123 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

A pseudogene in strain MG1655 / ATCC 700926, but not its parent MG1655 / ATCC 47076. In ATCC 700926 IS1I has recently inserted into the gene (PubMed:22081388).1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.