Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative cytochrome bd-II ubiquinol oxidase subunit AppX

Gene

appX

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Might be part of cytochrome bd-II oxidase (appB and appC). Able to restore reductant resistance to a cydX deletion mutant upon overexpression. CyxD and this protein may have some functional overlap.1 Publication

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-2817.

Protein family/group databases

TCDBi9.B.85.1.2. the 1 tms cydx/appx cytochrome bd oxidase activating (cydx/appx) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative cytochrome bd-II ubiquinol oxidase subunit AppX
Gene namesi
Name:appX
Synonyms:yccB
Ordered Locus Names:b4592, JW0961.1
ORF Names:b0979.1
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11114. appX.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei4 – 2421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • cell outer membrane Source: EcoCyc
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Not essential, no change in sensitivity to reductant (beta-mercaptoethanol).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3030Putative cytochrome bd-II ubiquinol oxidase subunit AppXPRO_0000168781Add
BLAST

Proteomic databases

PaxDbiP24244.

Expressioni

Inductioni

Expressed during stationary phase (PubMed:19121005) and in minimal glycerol medium, at 45 degrees Celsius, strongly (30-fold) induced in low oxygen (PubMed:19734316) (at protein level).2 Publications

Interactioni

Subunit structurei

Able to interact with CydA and CydB upon overexpression.

Protein-protein interaction databases

STRINGi511145.b4592.

Structurei

3D structure databases

ProteinModelPortaliP24244.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000009400.
OrthoDBiEOG6FRD42.

Family and domain databases

InterProiIPR012994. YbgT_YccB.
[Graphical view]
PfamiPF08173. YbgT_YccB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24244-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30
MWYLLWFVGI LLMCSLSTLV LVWLDPRLKS
Length:30
Mass (Da):3,597
Last modified:March 1, 1992 - v1
Checksum:i9E27FCD533329606
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58708 Genomic DNA. Translation: AAA72085.1.
S63811 Genomic DNA. Translation: AAB20287.1.
U00096 Genomic DNA. Translation: ABP93439.1.
AP009048 Genomic DNA. No translation available.
PIRiA36733.
RefSeqiWP_000270305.1. NZ_LN832404.1.
YP_001165313.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiABP93439; ABP93439; b4592.
GeneIDi948955.
KEGGieco:b4592.
PATRICi32117181. VBIEscCol129921_1014.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58708 Genomic DNA. Translation: AAA72085.1.
S63811 Genomic DNA. Translation: AAB20287.1.
U00096 Genomic DNA. Translation: ABP93439.1.
AP009048 Genomic DNA. No translation available.
PIRiA36733.
RefSeqiWP_000270305.1. NZ_LN832404.1.
YP_001165313.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP24244.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi511145.b4592.

Protein family/group databases

TCDBi9.B.85.1.2. the 1 tms cydx/appx cytochrome bd oxidase activating (cydx/appx) family.

Proteomic databases

PaxDbiP24244.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABP93439; ABP93439; b4592.
GeneIDi948955.
KEGGieco:b4592.
PATRICi32117181. VBIEscCol129921_1014.

Organism-specific databases

EchoBASEiEB1105.
EcoGeneiEG11114. appX.

Phylogenomic databases

HOGENOMiHOG000009400.
OrthoDBiEOG6FRD42.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-2817.

Miscellaneous databases

PROiP24244.

Family and domain databases

InterProiIPR012994. YbgT_YccB.
[Graphical view]
PfamiPF08173. YbgT_YccB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The complete nucleotide sequence of the Escherichia coli gene appA reveals significant homology between pH 2.5 acid phosphatase and glucose-1-phosphatase."
    Dassa J., Marck C., Boquet P.L.
    J. Bacteriol. 172:5497-5500(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "A new oxygen-regulated operon in Escherichia coli comprises the genes for a putative third cytochrome oxidase and for pH 2.5 acid phosphatase (appA)."
    Dassa J., Fsihi H., Marck C., Dion M., Kieffer-Bontemps M., Boquet P.L.
    Mol. Gen. Genet. 229:341-352(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Small membrane proteins found by comparative genomics and ribosome binding site models."
    Hemm M.R., Paul B.J., Schneider T.D., Storz G., Rudd K.E.
    Mol. Microbiol. 70:1487-1501(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Small stress response proteins in Escherichia coli: proteins missed by classical proteomic studies."
    Hemm M.R., Paul B.J., Miranda-Rios J., Zhang A., Soltanzad N., Storz G.
    J. Bacteriol. 192:46-58(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Membrane localization of small proteins in Escherichia coli."
    Fontaine F., Fuchs R.T., Storz G.
    J. Biol. Chem. 286:32464-32474(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TOPOLOGY.
    Strain: K12 / MG1655 / ATCC 47076.
  8. "The Escherichia coli CydX protein is a member of the CydAB cytochrome bd oxidase complex and is required for cytochrome bd oxidase activity."
    Vanorsdel C.E., Bhatt S., Allen R.J., Brenner E.P., Hobson J.J., Jamil A., Haynes B.M., Genson A.M., Hemm M.R.
    J. Bacteriol. 195:3640-3650(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, POSSIBLE INTERACTION WITH CYDA AND CYDB, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiAPPX_ECOLI
AccessioniPrimary (citable) accession number: P24244
Secondary accession number(s): A5A610
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 11, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.