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Protein

HTH-type transcriptional regulator AscG

Gene

ascG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi4 – 2320H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • sequence-specific DNA binding Source: EcoCyc
  • transcription factor activity, sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

  • negative regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10087-MONOMER.
ECOL316407:JW5434-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator AscG
Alternative name(s):
Cryptic asc operon repressor
Gene namesi
Name:ascG
Ordered Locus Names:b2714, JW5434
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10087. ascG.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 336336HTH-type transcriptional regulator AscGPRO_0000107923Add
BLAST

Proteomic databases

PaxDbiP24242.

Interactioni

Protein-protein interaction databases

BioGridi4259426. 8 interactions.
IntActiP24242. 14 interactions.
STRINGi511145.b2714.

Structurei

Secondary structure

1
336
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi61 – 666Combined sources
Helixi68 – 703Combined sources
Helixi76 – 8914Combined sources
Beta strandi93 – 975Combined sources
Helixi103 – 11513Combined sources
Beta strandi119 – 1246Combined sources
Beta strandi126 – 1283Combined sources
Helixi130 – 1389Combined sources
Beta strandi144 – 1485Combined sources
Beta strandi152 – 1543Combined sources
Helixi155 – 1573Combined sources
Helixi163 – 17614Combined sources
Beta strandi181 – 1855Combined sources
Helixi192 – 20615Combined sources
Turni207 – 2093Combined sources
Helixi214 – 2163Combined sources
Helixi224 – 23512Combined sources
Beta strandi242 – 2487Combined sources
Helixi249 – 26214Combined sources
Turni266 – 2694Combined sources
Beta strandi271 – 2766Combined sources
Helixi281 – 2833Combined sources
Beta strandi284 – 2863Combined sources
Beta strandi289 – 2924Combined sources
Helixi295 – 31016Combined sources
Beta strandi324 – 3263Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3BRQX-ray2.00A/B43-336[»]
3DBIX-ray2.45A/B/C2-336[»]
ProteinModelPortaliP24242.
SMRiP24242. Positions 1-334.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24242.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 5655HTH lacI-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH lacI-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105DDV. Bacteria.
COG1609. LUCA.
HOGENOMiHOG000220179.
InParanoidiP24242.
KOiK03487.
OMAiHGVYFSE.

Family and domain databases

CDDicd01392. HTH_LacI. 1 hit.
Gene3Di1.10.260.40. 1 hit.
InterProiIPR000843. HTH_LacI.
IPR010982. Lambda_DNA-bd_dom.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00356. LacI. 1 hit.
[Graphical view]
SMARTiSM00354. HTH_LACI. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF53822. SSF53822. 1 hit.
PROSITEiPS00356. HTH_LACI_1. 1 hit.
PS50932. HTH_LACI_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24242-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTMLEVAKR AGVSKATVSR VLSGNGYVSQ ETKDRVFQAV EESGYRPNLL
60 70 80 90 100
ARNLSAKSTQ TLGLVVTNTL YHGIYFSELL FHAARMAEEK GRQLLLADGK
110 120 130 140 150
HSAEEERQAI QYLLDLRCDA IMIYPRFLSV DEIDDIIDAH SQPIMVLNRR
160 170 180 190 200
LRKNSSHSVW CDHKQTSFNA VAELINAGHQ EIAFLTGSMD SPTSIERLAG
210 220 230 240 250
YKDALAQHGI ALNEKLIANG KWTPASGAEG VEMLLERGAK FSALVASNDD
260 270 280 290 300
MAIGAMKALH ERGVAVPEQV SVIGFDDIAI APYTVPALSS VKIPVTEMIQ
310 320 330
EIIGRLIFML DGGDFSPPKT FSGKLIRRDS LIAPSR
Length:336
Mass (Da):36,813
Last modified:November 16, 2011 - v4
Checksum:iA2825E9E4DF6A261
GO

Sequence cautioni

The sequence AAA16428 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAA69224 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAE76791 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti207 – 23024QHGIA…SGAEG → SMVLRSMKNLSLTVNGRLPA GRR in AAA16428 (PubMed:1630307).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73326 Unassigned DNA. Translation: AAA16428.1. Different initiation.
U29579 Genomic DNA. Translation: AAA69224.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75756.2.
AP009048 Genomic DNA. Translation: BAE76791.1. Different initiation.
PIRiF65051.
RefSeqiNP_417194.2. NC_000913.3.
WP_001394680.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75756; AAC75756; b2714.
BAE76791; BAE76791; BAE76791.
GeneIDi947305.
KEGGiecj:JW5434.
eco:b2714.
PATRICi32120828. VBIEscCol129921_2806.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73326 Unassigned DNA. Translation: AAA16428.1. Different initiation.
U29579 Genomic DNA. Translation: AAA69224.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75756.2.
AP009048 Genomic DNA. Translation: BAE76791.1. Different initiation.
PIRiF65051.
RefSeqiNP_417194.2. NC_000913.3.
WP_001394680.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3BRQX-ray2.00A/B43-336[»]
3DBIX-ray2.45A/B/C2-336[»]
ProteinModelPortaliP24242.
SMRiP24242. Positions 1-334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259426. 8 interactions.
IntActiP24242. 14 interactions.
STRINGi511145.b2714.

Proteomic databases

PaxDbiP24242.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75756; AAC75756; b2714.
BAE76791; BAE76791; BAE76791.
GeneIDi947305.
KEGGiecj:JW5434.
eco:b2714.
PATRICi32120828. VBIEscCol129921_2806.

Organism-specific databases

EchoBASEiEB0085.
EcoGeneiEG10087. ascG.

Phylogenomic databases

eggNOGiENOG4105DDV. Bacteria.
COG1609. LUCA.
HOGENOMiHOG000220179.
InParanoidiP24242.
KOiK03487.
OMAiHGVYFSE.

Enzyme and pathway databases

BioCyciEcoCyc:EG10087-MONOMER.
ECOL316407:JW5434-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP24242.
PROiP24242.

Family and domain databases

CDDicd01392. HTH_LacI. 1 hit.
Gene3Di1.10.260.40. 1 hit.
InterProiIPR000843. HTH_LacI.
IPR010982. Lambda_DNA-bd_dom.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF00356. LacI. 1 hit.
[Graphical view]
SMARTiSM00354. HTH_LACI. 1 hit.
[Graphical view]
SUPFAMiSSF47413. SSF47413. 1 hit.
SSF53822. SSF53822. 1 hit.
PROSITEiPS00356. HTH_LACI_1. 1 hit.
PS50932. HTH_LACI_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASCG_ECOLI
AccessioniPrimary (citable) accession number: P24242
Secondary accession number(s): Q2MAB5, Q46879
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 16, 2011
Last modified: September 7, 2016
This is version 128 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.