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Protein

HTH-type transcriptional regulator AscG

Gene

ascG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi4 – 23H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • DNA binding transcription factor activity Source: EcoCyc
  • sequence-specific DNA binding Source: EcoCyc

GO - Biological processi

  • negative regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: EcoCyc

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciEcoCyc:EG10087-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator AscG
Alternative name(s):
Cryptic asc operon repressor
Gene namesi
Name:ascG
Ordered Locus Names:b2714, JW5434
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10087 ascG

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001079231 – 336HTH-type transcriptional regulator AscGAdd BLAST336

Proteomic databases

PaxDbiP24242
PRIDEiP24242

Interactioni

Protein-protein interaction databases

BioGridi4259426, 8 interactors
IntActiP24242, 14 interactors
STRINGi316385.ECDH10B_2882

Structurei

Secondary structure

1336
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi61 – 66Combined sources6
Helixi68 – 70Combined sources3
Helixi76 – 89Combined sources14
Beta strandi93 – 97Combined sources5
Helixi103 – 115Combined sources13
Beta strandi119 – 124Combined sources6
Beta strandi126 – 128Combined sources3
Helixi130 – 138Combined sources9
Beta strandi144 – 148Combined sources5
Beta strandi152 – 154Combined sources3
Helixi155 – 157Combined sources3
Helixi163 – 176Combined sources14
Beta strandi181 – 185Combined sources5
Helixi192 – 206Combined sources15
Turni207 – 209Combined sources3
Helixi214 – 216Combined sources3
Helixi224 – 235Combined sources12
Beta strandi242 – 248Combined sources7
Helixi249 – 262Combined sources14
Turni266 – 269Combined sources4
Beta strandi271 – 276Combined sources6
Helixi281 – 283Combined sources3
Beta strandi284 – 286Combined sources3
Beta strandi289 – 292Combined sources4
Helixi295 – 310Combined sources16
Beta strandi324 – 326Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BRQX-ray2.00A/B43-336[»]
3DBIX-ray2.45A/B/C2-336[»]
ProteinModelPortaliP24242
SMRiP24242
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24242

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 56HTH lacI-typePROSITE-ProRule annotationAdd BLAST55

Phylogenomic databases

eggNOGiENOG4105DDV Bacteria
COG1609 LUCA
HOGENOMiHOG000220179
InParanoidiP24242
KOiK03487
OMAiLYHGVYF

Family and domain databases

CDDicd01392 HTH_LacI, 1 hit
InterProiView protein in InterPro
IPR000843 HTH_LacI
IPR010982 Lambda_DNA-bd_dom_sf
IPR028082 Peripla_BP_I
PfamiView protein in Pfam
PF00356 LacI, 1 hit
SMARTiView protein in SMART
SM00354 HTH_LACI, 1 hit
SUPFAMiSSF47413 SSF47413, 1 hit
SSF53822 SSF53822, 1 hit
PROSITEiView protein in PROSITE
PS00356 HTH_LACI_1, 1 hit
PS50932 HTH_LACI_2, 1 hit

Sequencei

Sequence statusi: Complete.

P24242-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTMLEVAKR AGVSKATVSR VLSGNGYVSQ ETKDRVFQAV EESGYRPNLL
60 70 80 90 100
ARNLSAKSTQ TLGLVVTNTL YHGIYFSELL FHAARMAEEK GRQLLLADGK
110 120 130 140 150
HSAEEERQAI QYLLDLRCDA IMIYPRFLSV DEIDDIIDAH SQPIMVLNRR
160 170 180 190 200
LRKNSSHSVW CDHKQTSFNA VAELINAGHQ EIAFLTGSMD SPTSIERLAG
210 220 230 240 250
YKDALAQHGI ALNEKLIANG KWTPASGAEG VEMLLERGAK FSALVASNDD
260 270 280 290 300
MAIGAMKALH ERGVAVPEQV SVIGFDDIAI APYTVPALSS VKIPVTEMIQ
310 320 330
EIIGRLIFML DGGDFSPPKT FSGKLIRRDS LIAPSR
Length:336
Mass (Da):36,813
Last modified:November 16, 2011 - v4
Checksum:iA2825E9E4DF6A261
GO

Sequence cautioni

The sequence AAA16428 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAA69224 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAE76791 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti207 – 230QHGIA…SGAEG → SMVLRSMKNLSLTVNGRLPA GRR in AAA16428 (PubMed:1630307).CuratedAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73326 Unassigned DNA Translation: AAA16428.1 Different initiation.
U29579 Genomic DNA Translation: AAA69224.1 Different initiation.
U00096 Genomic DNA Translation: AAC75756.2
AP009048 Genomic DNA Translation: BAE76791.1 Different initiation.
PIRiF65051
RefSeqiNP_417194.2, NC_000913.3
WP_001394680.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75756; AAC75756; b2714
BAE76791; BAE76791; BAE76791
GeneIDi947305
KEGGiecj:JW5434
eco:b2714
PATRICifig|511145.12.peg.2806

Similar proteinsi

Entry informationi

Entry nameiASCG_ECOLI
AccessioniPrimary (citable) accession number: P24242
Secondary accession number(s): Q2MAB5, Q46879
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 16, 2011
Last modified: March 28, 2018
This is version 138 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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