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Protein

PTS system arbutin-, cellobiose-, and salicin-specific EIIBC component

Gene

ascF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in arbutin, cellobiose, and salicin transport.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei28Phosphocysteine intermediate; for EIIB activityPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:ASCF-MONOMER
MetaCyc:ASCF-MONOMER

Protein family/group databases

TCDBi4.A.1.2.3 the pts glucose-glucoside (glc) family

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system arbutin-, cellobiose-, and salicin-specific EIIBC component
Alternative name(s):
EIIBC-Asc
Short name:
EII-Asc
Including the following 2 domains:
Arbutin-, cellobiose-, and salicin-specific phosphotransferase enzyme IIB component (EC:2.7.1.-)
Alternative name(s):
PTS system arbutin-, cellobiose-, and salicin-specific EIIB component
Arbutin, cellobiose, and salicin permease IIC component
Alternative name(s):
PTS system arbutin-, cellobiose-, and salicin-specific EIIC component
Gene namesi
Name:ascF
Ordered Locus Names:b2715, JW5435
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10086 ascF

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei102 – 122HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei147 – 167HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei177 – 197HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei207 – 227HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei254 – 274HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei285 – 305HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei330 – 350HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei363 – 383HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei389 – 409HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei433 – 453HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001864981 – 485PTS system arbutin-, cellobiose-, and salicin-specific EIIBC componentAdd BLAST485

Proteomic databases

PaxDbiP24241
PRIDEiP24241

Interactioni

Protein-protein interaction databases

BioGridi4261308, 9 interactors
STRINGi316385.ECDH10B_2883

Structurei

3D structure databases

ProteinModelPortaliP24241
SMRiP24241
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 88PTS EIIB type-1PROSITE-ProRule annotationAdd BLAST88
Domaini108 – 470PTS EIIC type-1PROSITE-ProRule annotationAdd BLAST363

Domaini

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C5Y Bacteria
COG1263 LUCA
COG1264 LUCA
HOGENOMiHOG000102023
KOiK02752
K02753
OMAiAMILEMT
PhylomeDBiP24241

Family and domain databases

CDDicd00212 PTS_IIB_glc, 1 hit
Gene3Di3.30.1360.60, 1 hit
InterProiView protein in InterPro
IPR036878 Glu_permease_IIB
IPR018113 PTrfase_EIIB_Cys
IPR003352 PTS_EIIC
IPR013013 PTS_EIIC_1
IPR001996 PTS_IIB_1
IPR004719 PTS_maltose/Glc_sub_IIC
PfamiView protein in Pfam
PF00367 PTS_EIIB, 1 hit
PF02378 PTS_EIIC, 1 hit
SUPFAMiSSF55604 SSF55604, 1 hit
TIGRFAMsiTIGR00852 pts-Glc, 1 hit
PROSITEiView protein in PROSITE
PS51098 PTS_EIIB_TYPE_1, 1 hit
PS01035 PTS_EIIB_TYPE_1_CYS, 1 hit
PS51103 PTS_EIIC_TYPE_1, 1 hit

Sequencei

Sequence statusi: Complete.

P24241-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKNYAALAR SVIAALGGVD NISAVTHCMT RLRFVIKDDA LIDSPTLKTI
60 70 80 90 100
PGVLGVVRSD NQCQVIIGNT VSQAFQEVVS LLPGDMQPAQ PVGKPKLTLR
110 120 130 140 150
RIGAGILDAL IGTMSPLIPA IIGGSMVKLL AMILEMSGVL TKGSPTLTIL
160 170 180 190 200
NVIGDGAFFF LPLMVAASAA IKFKTNMSLA IAIAGVLVHP SFIELMAKAA
210 220 230 240 250
QGEHVEFALI PVTAVKYTYT VIPALVMTWC LSYIERWVDS ITPAVTKNFL
260 270 280 290 300
KPMLIVLIAA PLAILLIGPI GIWIGSAISA LVYTIHGYLG WLSVAIMGAL
310 320 330 340 350
WPLLVMTGMH RVFTPTIIQT IAETGKEGMV MPSEIGANLS LGGSSLAVAW
360 370 380 390 400
KTKNPELRQT ALAAAASAIM AGISEPALYG VAIRLKRPLI ASLISGFICG
410 420 430 440 450
AVAGMAGLAS HSMAAPGLFT SVQFFDPANP MSIVWVFAVM ALAVVLSFIL
460 470 480
TLLLGFEDIP VEEAAAQARK YQSVQPTVAK EVSLN
Length:485
Mass (Da):51,026
Last modified:October 11, 2004 - v3
Checksum:iE53627EB31B9523C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti167 – 170ASAA → HLPR in AAA69225 (PubMed:9278503).Curated4
Sequence conflicti167A → Q in AAA16429 (PubMed:1630307).Curated1
Sequence conflicti311R → H in AAA16429 (PubMed:1630307).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73326 Unassigned DNA Translation: AAA16429.1
U29579 Genomic DNA Translation: AAA69225.1
U00096 Genomic DNA Translation: AAT48150.1
AP009048 Genomic DNA Translation: BAE76792.1
PIRiG65051
RefSeqiWP_001107828.1, NZ_LN832404.1
YP_026182.1, NC_000913.3

Genome annotation databases

EnsemblBacteriaiAAT48150; AAT48150; b2715
BAE76792; BAE76792; BAE76792
GeneIDi947154
KEGGiecj:JW5435
eco:b2715
PATRICifig|1411691.4.peg.4026

Similar proteinsi

Entry informationi

Entry nameiPTIBC_ECOLI
AccessioniPrimary (citable) accession number: P24241
Secondary accession number(s): Q2MAB4, Q46880, Q6BF64
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: October 11, 2004
Last modified: March 28, 2018
This is version 150 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

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