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Protein

PTS system arbutin-, cellobiose-, and salicin-specific EIIBC component

Gene

ascF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in arbutin, cellobiose, and salicin transport.

Catalytic activityi

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei28 – 281Phosphocysteine intermediate; for EIIB activityPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:ASCF-MONOMER.
ECOL316407:JW5435-MONOMER.

Protein family/group databases

TCDBi4.A.1.2.3. the pts glucose-glucoside (glc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system arbutin-, cellobiose-, and salicin-specific EIIBC component
Alternative name(s):
EIIBC-Asc
Short name:
EII-Asc
Including the following 2 domains:
Arbutin-, cellobiose-, and salicin-specific phosphotransferase enzyme IIB component (EC:2.7.1.-)
Alternative name(s):
PTS system arbutin-, cellobiose-, and salicin-specific EIIB component
Arbutin, cellobiose, and salicin permease IIC component
Alternative name(s):
PTS system arbutin-, cellobiose-, and salicin-specific EIIC component
Gene namesi
Name:ascF
Ordered Locus Names:b2715, JW5435
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10086. ascF.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei102 – 12221HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei147 – 16721HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei177 – 19721HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei207 – 22721HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei254 – 27421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei285 – 30521HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei330 – 35021HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei363 – 38321HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei389 – 40921HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei433 – 45321HelicalPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 485485PTS system arbutin-, cellobiose-, and salicin-specific EIIBC componentPRO_0000186498Add
BLAST

Proteomic databases

PaxDbiP24241.
PRIDEiP24241.

Interactioni

Protein-protein interaction databases

BioGridi4261308. 9 interactions.
STRINGi511145.b2715.

Structurei

3D structure databases

ProteinModelPortaliP24241.
SMRiP24241. Positions 2-78.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 8888PTS EIIB type-1PROSITE-ProRule annotationAdd
BLAST
Domaini108 – 470363PTS EIIC type-1PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

Sequence similaritiesi

Contains 1 PTS EIIB type-1 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIC type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C5Y. Bacteria.
COG1263. LUCA.
COG1264. LUCA.
HOGENOMiHOG000102023.
KOiK02752.
K02753.
OMAiILTVIGD.
PhylomeDBiP24241.

Family and domain databases

CDDicd00212. PTS_IIB_glc. 1 hit.
Gene3Di3.30.1360.60. 1 hit.
InterProiIPR018113. PTrfase_EIIB_Cys.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
IPR004719. PTS_maltose/Glc_sub_IIC.
[Graphical view]
PfamiPF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMiSSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00852. pts-Glc. 1 hit.
PROSITEiPS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24241-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKNYAALAR SVIAALGGVD NISAVTHCMT RLRFVIKDDA LIDSPTLKTI
60 70 80 90 100
PGVLGVVRSD NQCQVIIGNT VSQAFQEVVS LLPGDMQPAQ PVGKPKLTLR
110 120 130 140 150
RIGAGILDAL IGTMSPLIPA IIGGSMVKLL AMILEMSGVL TKGSPTLTIL
160 170 180 190 200
NVIGDGAFFF LPLMVAASAA IKFKTNMSLA IAIAGVLVHP SFIELMAKAA
210 220 230 240 250
QGEHVEFALI PVTAVKYTYT VIPALVMTWC LSYIERWVDS ITPAVTKNFL
260 270 280 290 300
KPMLIVLIAA PLAILLIGPI GIWIGSAISA LVYTIHGYLG WLSVAIMGAL
310 320 330 340 350
WPLLVMTGMH RVFTPTIIQT IAETGKEGMV MPSEIGANLS LGGSSLAVAW
360 370 380 390 400
KTKNPELRQT ALAAAASAIM AGISEPALYG VAIRLKRPLI ASLISGFICG
410 420 430 440 450
AVAGMAGLAS HSMAAPGLFT SVQFFDPANP MSIVWVFAVM ALAVVLSFIL
460 470 480
TLLLGFEDIP VEEAAAQARK YQSVQPTVAK EVSLN
Length:485
Mass (Da):51,026
Last modified:October 11, 2004 - v3
Checksum:iE53627EB31B9523C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti167 – 1704ASAA → HLPR in AAA69225 (PubMed:9278503).Curated
Sequence conflicti167 – 1671A → Q in AAA16429 (PubMed:1630307).Curated
Sequence conflicti311 – 3111R → H in AAA16429 (PubMed:1630307).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73326 Unassigned DNA. Translation: AAA16429.1.
U29579 Genomic DNA. Translation: AAA69225.1.
U00096 Genomic DNA. Translation: AAT48150.1.
AP009048 Genomic DNA. Translation: BAE76792.1.
PIRiG65051.
RefSeqiWP_001107828.1. NZ_LN832404.1.
YP_026182.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAT48150; AAT48150; b2715.
BAE76792; BAE76792; BAE76792.
GeneIDi947154.
KEGGiecj:JW5435.
eco:b2715.
PATRICi32120830. VBIEscCol129921_2807.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73326 Unassigned DNA. Translation: AAA16429.1.
U29579 Genomic DNA. Translation: AAA69225.1.
U00096 Genomic DNA. Translation: AAT48150.1.
AP009048 Genomic DNA. Translation: BAE76792.1.
PIRiG65051.
RefSeqiWP_001107828.1. NZ_LN832404.1.
YP_026182.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP24241.
SMRiP24241. Positions 2-78.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261308. 9 interactions.
STRINGi511145.b2715.

Protein family/group databases

TCDBi4.A.1.2.3. the pts glucose-glucoside (glc) family.

Proteomic databases

PaxDbiP24241.
PRIDEiP24241.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT48150; AAT48150; b2715.
BAE76792; BAE76792; BAE76792.
GeneIDi947154.
KEGGiecj:JW5435.
eco:b2715.
PATRICi32120830. VBIEscCol129921_2807.

Organism-specific databases

EchoBASEiEB0084.
EcoGeneiEG10086. ascF.

Phylogenomic databases

eggNOGiENOG4105C5Y. Bacteria.
COG1263. LUCA.
COG1264. LUCA.
HOGENOMiHOG000102023.
KOiK02752.
K02753.
OMAiILTVIGD.
PhylomeDBiP24241.

Enzyme and pathway databases

BioCyciEcoCyc:ASCF-MONOMER.
ECOL316407:JW5435-MONOMER.

Miscellaneous databases

PROiP24241.

Family and domain databases

CDDicd00212. PTS_IIB_glc. 1 hit.
Gene3Di3.30.1360.60. 1 hit.
InterProiIPR018113. PTrfase_EIIB_Cys.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
IPR004719. PTS_maltose/Glc_sub_IIC.
[Graphical view]
PfamiPF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMiSSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00852. pts-Glc. 1 hit.
PROSITEiPS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTIBC_ECOLI
AccessioniPrimary (citable) accession number: P24241
Secondary accession number(s): Q2MAB4, Q46880, Q6BF64
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: October 11, 2004
Last modified: September 7, 2016
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.