Reviewed, UniProtKB/Swiss-Prot P24240 (ASCB_ECOLI)

Names and origin

Protein names

Recommended name:
    6-phospho-beta-glucosidase ascB
    EC=3.2.1.86

Gene names

Name:	ascB
Ordered Locus Names:	b2716, JW2686

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	474 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can hydrolyze salicin, cellobiose, and probably arbutin.
Catalytic activity	6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	6-phospho-beta-glucosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 474	474	6-phospho-beta-glucosidase ascB		PRO_0000063895
Sites
	Active site	180	1	Proton donor Potential
	Active site	372	1	Nucleophile By similarity
Experimental info
	Sequence conflict	405 – 406	2	EA → GT in AAA16430. Ref.1
	Sequence conflict	428	1	S → C in AAA16430. Ref.1
	Sequence conflict	455 – 456	2	RK → HR in AAA16430. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P24240-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 02ACE6BEBF211011	FASTA	474	53,935
	10 20 30 40 50 60 MSVFPESFLW GGALAANQSE GAFREGDKGL TTVDMIPHGE HRMAVKLGLE KRFQLRDDEF 70 80 90 100 110 120 YPSHEATDFY HRYKEDIALM AEMGFKVFRT SIAWSRLFPQ GDEITPNQQG IAFYRSVFEE 130 140 150 160 170 180 CKKYGIEPLV TLCHFDVPMH LVTEYGSWRN RKLVEFFSRY ARTCFEAFDG LVKYWLTFNE 190 200 210 220 230 240 INIMLHSPFS GAGLVFEEGE NQDQVKYQAA HHQLVASALA TKIAHEVNPQ NQVGCMLAGG 250 260 270 280 290 300 NFYPYSCKPE DVWAALEKDR ENLFFIDVQA RGTYPAYSAR VFREKGVTIN KAPGDDEILK 310 320 330 340 350 360 NTVDFVSFSY YASRCASAEM NANNSSAANV VKSLRNPYLQ VSDWGWGIDP LGLRITMNMM 370 380 390 400 410 420 YDRYQKPLFL VENGLGAKDE FAANGEINDD YRISYLREHI RAMGEAIADG IPLMGYTTWG 430 440 450 460 470 CIDLVSASTG EMSKRYGFVF VDRDDAGNGT LTRTRKKSFW WYKKVIASNG EDLE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence, function, activation, and evolution of the cryptic asc operon of Escherichia coli K12." Hall B.G., Xu L. Mol. Biol. Evol. 9:688-706(1992) [PubMed: 1630307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M73326 Unassigned DNA. Translation: AAA16430.1. U29579 Genomic DNA. Translation: AAA69226.1. Different initiation. U00096 Genomic DNA. Translation: AAC75758.1. AP009048 Genomic DNA. Translation: BAE76793.1.
PIR	H65051.
RefSeq	AP_003283.1. NP_417196.1.
3D structure databases
HSSP	HSSP built from PDB template 1PBG based on UniProtKB P11546.
ModBase	Search...
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Genome annotation databases
GeneID	947460.
GenomeReviews	Gene locus JW2686 in contig AP009048_GR. Gene locus b2716 in contig U00096_GR.
KEGG	ecj:JW2686. eco:b2716.
Organism-specific databases
EchoBASE	EB0083.
EcoGene	EG10085. ascB.
CMR	Search...
Phylogenomic databases
HOGENOM	P24240.
OMA	P24240. NQSEGAY.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10085-MON. MetaCyc:EG10085-MON.
Family and domain databases
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 2 hits. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASCB_ECOLI

Accession

Primary (citable) accession number: P24240
Secondary accession number(s): P78104, Q2MAB3, Q59375

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)