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Protein

6-phospho-beta-glucosidase AscB

Gene

ascB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Can hydrolyze salicin, cellobiose, and probably arbutin.

Catalytic activityi

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei180Proton donorSequence analysis1
Active sitei372NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • cellobiose catabolic process Source: EcoCyc
  • glycosyl compound metabolic process Source: GO_Central

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:EG10085-MONOMER.
MetaCyc:EG10085-MONOMER.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-beta-glucosidase AscB (EC:3.2.1.86)
Gene namesi
Name:ascB
Ordered Locus Names:b2716, JW2686
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10085. ascB.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000638951 – 4746-phospho-beta-glucosidase AscBAdd BLAST474

Proteomic databases

PaxDbiP24240.
PRIDEiP24240.

Interactioni

Protein-protein interaction databases

BioGridi4259428. 18 interactors.
IntActiP24240. 5 interactors.
STRINGi316385.ECDH10B_2884.

Structurei

3D structure databases

ProteinModelPortaliP24240.
SMRiP24240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 1 family.Curated

Phylogenomic databases

eggNOGiENOG4105CS2. Bacteria.
COG2723. LUCA.
HOGENOMiHOG000088631.
InParanoidiP24240.
KOiK01223.
PhylomeDBiP24240.

Family and domain databases

InterProiView protein in InterPro
IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR033132. Glyco_hydro_1_N_CS.
IPR017853. Glycoside_hydrolase_SF.
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiView protein in Pfam
PF00232. Glyco_hydro_1. 1 hit.
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiView protein in PROSITE
PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P24240-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVFPESFLW GGALAANQSE GAFREGDKGL TTVDMIPHGE HRMAVKLGLE
60 70 80 90 100
KRFQLRDDEF YPSHEATDFY HRYKEDIALM AEMGFKVFRT SIAWSRLFPQ
110 120 130 140 150
GDEITPNQQG IAFYRSVFEE CKKYGIEPLV TLCHFDVPMH LVTEYGSWRN
160 170 180 190 200
RKLVEFFSRY ARTCFEAFDG LVKYWLTFNE INIMLHSPFS GAGLVFEEGE
210 220 230 240 250
NQDQVKYQAA HHQLVASALA TKIAHEVNPQ NQVGCMLAGG NFYPYSCKPE
260 270 280 290 300
DVWAALEKDR ENLFFIDVQA RGTYPAYSAR VFREKGVTIN KAPGDDEILK
310 320 330 340 350
NTVDFVSFSY YASRCASAEM NANNSSAANV VKSLRNPYLQ VSDWGWGIDP
360 370 380 390 400
LGLRITMNMM YDRYQKPLFL VENGLGAKDE FAANGEINDD YRISYLREHI
410 420 430 440 450
RAMGEAIADG IPLMGYTTWG CIDLVSASTG EMSKRYGFVF VDRDDAGNGT
460 470
LTRTRKKSFW WYKKVIASNG EDLE
Length:474
Mass (Da):53,935
Last modified:November 1, 1997 - v2
Checksum:i02ACE6BEBF211011
GO

Sequence cautioni

P24240: The sequence AAA69226 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti405 – 406EA → GT in AAA16430 (PubMed:1630307).Curated2
Sequence conflicti428S → C in AAA16430 (PubMed:1630307).Curated1
Sequence conflicti455 – 456RK → HR in AAA16430 (PubMed:1630307).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73326 Unassigned DNA. Translation: AAA16430.1.
U29579 Genomic DNA. Translation: AAA69226.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75758.1.
AP009048 Genomic DNA. Translation: BAE76793.1.
PIRiH65051.
RefSeqiNP_417196.1. NC_000913.3.
WP_000110363.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75758; AAC75758; b2716.
BAE76793; BAE76793; BAE76793.
GeneIDi947460.
KEGGiecj:JW2686.
eco:b2716.
PATRICifig|1411691.4.peg.4025.

Similar proteinsi

Entry informationi

Entry nameiASCB_ECOLI
AccessioniPrimary (citable) accession number: P24240
Secondary accession number(s): P78104, Q2MAB3, Q59375
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 1, 1997
Last modified: October 25, 2017
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families