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P24240

- ASCB_ECOLI

UniProt

P24240 - ASCB_ECOLI

Protein

6-phospho-beta-glucosidase AscB

Gene

ascB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Can hydrolyze salicin, cellobiose, and probably arbutin.

    Catalytic activityi

    6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei180 – 1801Proton donorSequence Analysis
    Active sitei372 – 3721NucleophilePROSITE-ProRule annotation

    GO - Molecular functioni

    1. 6-phospho-beta-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10085-MONOMER.
    ECOL316407:JW2686-MONOMER.
    MetaCyc:EG10085-MONOMER.

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    6-phospho-beta-glucosidase AscB (EC:3.2.1.86)
    Gene namesi
    Name:ascB
    Ordered Locus Names:b2716, JW2686
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10085. ascB.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4744746-phospho-beta-glucosidase AscBPRO_0000063895Add
    BLAST

    Proteomic databases

    PRIDEiP24240.

    Expressioni

    Gene expression databases

    GenevestigatoriP24240.

    Interactioni

    Protein-protein interaction databases

    IntActiP24240. 5 interactions.
    STRINGi511145.b2716.

    Structurei

    3D structure databases

    ProteinModelPortaliP24240.
    SMRiP24240. Positions 1-473.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088631.
    KOiK01223.
    OMAiRDLFNEC.
    OrthoDBiEOG6F81PM.
    PhylomeDBiP24240.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24240-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVFPESFLW GGALAANQSE GAFREGDKGL TTVDMIPHGE HRMAVKLGLE    50
    KRFQLRDDEF YPSHEATDFY HRYKEDIALM AEMGFKVFRT SIAWSRLFPQ 100
    GDEITPNQQG IAFYRSVFEE CKKYGIEPLV TLCHFDVPMH LVTEYGSWRN 150
    RKLVEFFSRY ARTCFEAFDG LVKYWLTFNE INIMLHSPFS GAGLVFEEGE 200
    NQDQVKYQAA HHQLVASALA TKIAHEVNPQ NQVGCMLAGG NFYPYSCKPE 250
    DVWAALEKDR ENLFFIDVQA RGTYPAYSAR VFREKGVTIN KAPGDDEILK 300
    NTVDFVSFSY YASRCASAEM NANNSSAANV VKSLRNPYLQ VSDWGWGIDP 350
    LGLRITMNMM YDRYQKPLFL VENGLGAKDE FAANGEINDD YRISYLREHI 400
    RAMGEAIADG IPLMGYTTWG CIDLVSASTG EMSKRYGFVF VDRDDAGNGT 450
    LTRTRKKSFW WYKKVIASNG EDLE 474
    Length:474
    Mass (Da):53,935
    Last modified:November 1, 1997 - v2
    Checksum:i02ACE6BEBF211011
    GO

    Sequence cautioni

    The sequence AAA69226.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti405 – 4062EA → GT in AAA16430. (PubMed:1630307)Curated
    Sequence conflicti428 – 4281S → C in AAA16430. (PubMed:1630307)Curated
    Sequence conflicti455 – 4562RK → HR in AAA16430. (PubMed:1630307)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73326 Unassigned DNA. Translation: AAA16430.1.
    U29579 Genomic DNA. Translation: AAA69226.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC75758.1.
    AP009048 Genomic DNA. Translation: BAE76793.1.
    PIRiH65051.
    RefSeqiNP_417196.1. NC_000913.3.
    YP_490925.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75758; AAC75758; b2716.
    BAE76793; BAE76793; BAE76793.
    GeneIDi12930434.
    947460.
    KEGGiecj:Y75_p2654.
    eco:b2716.
    PATRICi32120832. VBIEscCol129921_2808.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73326 Unassigned DNA. Translation: AAA16430.1 .
    U29579 Genomic DNA. Translation: AAA69226.1 . Different initiation.
    U00096 Genomic DNA. Translation: AAC75758.1 .
    AP009048 Genomic DNA. Translation: BAE76793.1 .
    PIRi H65051.
    RefSeqi NP_417196.1. NC_000913.3.
    YP_490925.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P24240.
    SMRi P24240. Positions 1-473.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P24240. 5 interactions.
    STRINGi 511145.b2716.

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PRIDEi P24240.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75758 ; AAC75758 ; b2716 .
    BAE76793 ; BAE76793 ; BAE76793 .
    GeneIDi 12930434.
    947460.
    KEGGi ecj:Y75_p2654.
    eco:b2716.
    PATRICi 32120832. VBIEscCol129921_2808.

    Organism-specific databases

    EchoBASEi EB0083.
    EcoGenei EG10085. ascB.

    Phylogenomic databases

    eggNOGi COG2723.
    HOGENOMi HOG000088631.
    KOi K01223.
    OMAi RDLFNEC.
    OrthoDBi EOG6F81PM.
    PhylomeDBi P24240.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10085-MONOMER.
    ECOL316407:JW2686-MONOMER.
    MetaCyc:EG10085-MONOMER.

    Miscellaneous databases

    PROi P24240.

    Gene expression databases

    Genevestigatori P24240.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR018120. Glyco_hydro_1_AS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
    PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence, function, activation, and evolution of the cryptic asc operon of Escherichia coli K12."
      Hall B.G., Xu L.
      Mol. Biol. Evol. 9:688-706(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiASCB_ECOLI
    AccessioniPrimary (citable) accession number: P24240
    Secondary accession number(s): P78104, Q2MAB3, Q59375
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3