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P24240 (ASCB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
6-phospho-beta-glucosidase AscB

EC=3.2.1.86
Gene names
Name:ascB
Ordered Locus Names:b2716, JW2686
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Can hydrolyze salicin, cellobiose, and probably arbutin.

Catalytic activity

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Sequence caution

The sequence AAA69226.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phospho-beta-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4744746-phospho-beta-glucosidase AscB
PRO_0000063895

Sites

Active site1801Proton donor Potential
Active site3721Nucleophile By similarity

Experimental info

Sequence conflict405 – 4062EA → GT in AAA16430. Ref.1
Sequence conflict4281S → C in AAA16430. Ref.1
Sequence conflict455 – 4562RK → HR in AAA16430. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P24240 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 02ACE6BEBF211011

FASTA47453,935
        10         20         30         40         50         60 
MSVFPESFLW GGALAANQSE GAFREGDKGL TTVDMIPHGE HRMAVKLGLE KRFQLRDDEF 

        70         80         90        100        110        120 
YPSHEATDFY HRYKEDIALM AEMGFKVFRT SIAWSRLFPQ GDEITPNQQG IAFYRSVFEE 

       130        140        150        160        170        180 
CKKYGIEPLV TLCHFDVPMH LVTEYGSWRN RKLVEFFSRY ARTCFEAFDG LVKYWLTFNE 

       190        200        210        220        230        240 
INIMLHSPFS GAGLVFEEGE NQDQVKYQAA HHQLVASALA TKIAHEVNPQ NQVGCMLAGG 

       250        260        270        280        290        300 
NFYPYSCKPE DVWAALEKDR ENLFFIDVQA RGTYPAYSAR VFREKGVTIN KAPGDDEILK 

       310        320        330        340        350        360 
NTVDFVSFSY YASRCASAEM NANNSSAANV VKSLRNPYLQ VSDWGWGIDP LGLRITMNMM 

       370        380        390        400        410        420 
YDRYQKPLFL VENGLGAKDE FAANGEINDD YRISYLREHI RAMGEAIADG IPLMGYTTWG 

       430        440        450        460        470 
CIDLVSASTG EMSKRYGFVF VDRDDAGNGT LTRTRKKSFW WYKKVIASNG EDLE 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence, function, activation, and evolution of the cryptic asc operon of Escherichia coli K12."
Hall B.G., Xu L.
Mol. Biol. Evol. 9:688-706(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M73326 Unassigned DNA. Translation: AAA16430.1.
U29579 Genomic DNA. Translation: AAA69226.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75758.1.
AP009048 Genomic DNA. Translation: BAE76793.1.
PIRH65051.
RefSeqNP_417196.1. NC_000913.3.
YP_490925.1. NC_007779.1.

3D structure databases

ProteinModelPortalP24240.
SMRP24240. Positions 1-473.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP24240. 5 interactions.
STRING511145.b2716.

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEP24240.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75758; AAC75758; b2716.
BAE76793; BAE76793; BAE76793.
GeneID12930434.
947460.
KEGGecj:Y75_p2654.
eco:b2716.
PATRIC32120832. VBIEscCol129921_2808.

Organism-specific databases

EchoBASEEB0083.
EcoGeneEG10085. ascB.

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088631.
KOK01223.
OMARDLFNEC.
OrthoDBEOG6F81PM.
PhylomeDBP24240.

Enzyme and pathway databases

BioCycEcoCyc:EG10085-MONOMER.
ECOL316407:JW2686-MONOMER.
MetaCyc:EG10085-MONOMER.

Gene expression databases

GenevestigatorP24240.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP24240.

Entry information

Entry nameASCB_ECOLI
AccessionPrimary (citable) accession number: P24240
Secondary accession number(s): P78104, Q2MAB3, Q59375
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene