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Reviewed, UniProtKB/Swiss-Prot P24240 (ASCB_ECOLI)

Last modified June 16, 2009. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    6-phospho-beta-glucosidase ascB
    EC=3.2.1.86
Gene names
Name: ascB
Ordered Locus Names: b2716, JW2686
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Can hydrolyze salicin, cellobiose, and probably arbutin.

Catalytic activity

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular function6-phospho-beta-glucosidase activity

Inferred from electronic annotation. Source: EC

cation binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4744746-phospho-beta-glucosidase ascB
PRO_0000063895

Sites

Active site1801Proton donor Potential
Active site3721Nucleophile By similarity

Experimental info

Sequence conflict405 – 4062EA → GT in AAA16430. Ref.1
Sequence conflict4281S → C in AAA16430. Ref.1
Sequence conflict455 – 4562RK → HR in AAA16430. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P24240-1 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 02ACE6BEBF211011

FASTA47453,935
        10         20         30         40         50         60 
MSVFPESFLW GGALAANQSE GAFREGDKGL TTVDMIPHGE HRMAVKLGLE KRFQLRDDEF 

        70         80         90        100        110        120 
YPSHEATDFY HRYKEDIALM AEMGFKVFRT SIAWSRLFPQ GDEITPNQQG IAFYRSVFEE 

       130        140        150        160        170        180 
CKKYGIEPLV TLCHFDVPMH LVTEYGSWRN RKLVEFFSRY ARTCFEAFDG LVKYWLTFNE 

       190        200        210        220        230        240 
INIMLHSPFS GAGLVFEEGE NQDQVKYQAA HHQLVASALA TKIAHEVNPQ NQVGCMLAGG 

       250        260        270        280        290        300 
NFYPYSCKPE DVWAALEKDR ENLFFIDVQA RGTYPAYSAR VFREKGVTIN KAPGDDEILK 

       310        320        330        340        350        360 
NTVDFVSFSY YASRCASAEM NANNSSAANV VKSLRNPYLQ VSDWGWGIDP LGLRITMNMM 

       370        380        390        400        410        420 
YDRYQKPLFL VENGLGAKDE FAANGEINDD YRISYLREHI RAMGEAIADG IPLMGYTTWG 

       430        440        450        460        470 
CIDLVSASTG EMSKRYGFVF VDRDDAGNGT LTRTRKKSFW WYKKVIASNG EDLE 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence, function, activation, and evolution of the cryptic asc operon of Escherichia coli K12."
Hall B.G., Xu L.
Mol. Biol. Evol. 9:688-706(1992) [PubMed: 1630307] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

M73326 Unassigned DNA. Translation: AAA16430.1.
U29579 Genomic DNA. Translation: AAA69226.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75758.1.
AP009048 Genomic DNA. Translation: BAE76793.1.
PIRH65051.
RefSeqAP_003283.1.
NP_417196.1.

3D structure databases

HSSPHSSP built from PDB template 1PBG based on UniProtKB P11546.
ModBaseSearch...

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Genome annotation databases

GeneID947460.
GenomeReviewsGene locus JW2686 in contig AP009048_GR.
Gene locus b2716 in contig U00096_GR.
KEGGecj:JW2686.
eco:b2716.

Organism-specific databases

EchoBASEEB0083.
EcoGeneEG10085. ascB.
CMRSearch...

Phylogenomic databases

HOGENOMP24240.
OMAP24240. NQSEGAY.

Enzyme and pathway databases

BioCycEcoCyc:EG10085-MON.
MetaCyc:EG10085-MON.

Family and domain databases

InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_sg_catalytic.
[Graphical view]
Gene3DG3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHERPTHR10353. Glyco_hydro_1. 1 hit.
PfamPF00232. Glyco_hydro_1. 2 hits.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASCB_ECOLI
AccessionPrimary (citable) accession number: P24240
Secondary accession number(s): P78104, Q2MAB3, Q59375
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 1, 1997
Last modified: June 16, 2009
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents