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P24240

- ASCB_ECOLI

UniProt

P24240 - ASCB_ECOLI

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Protein
6-phospho-beta-glucosidase AscB
Gene
ascB, b2716, JW2686
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Can hydrolyze salicin, cellobiose, and probably arbutin.

Catalytic activityi

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H2O = D-glucose + D-glucose 6-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei180 – 1801Proton donor Reviewed prediction
Active sitei372 – 3721Nucleophile By similarity

GO - Molecular functioni

  1. 6-phospho-beta-glucosidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BioCyciEcoCyc:EG10085-MONOMER.
ECOL316407:JW2686-MONOMER.
MetaCyc:EG10085-MONOMER.

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phospho-beta-glucosidase AscB (EC:3.2.1.86)
Gene namesi
Name:ascB
Ordered Locus Names:b2716, JW2686
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10085. ascB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4744746-phospho-beta-glucosidase AscB
PRO_0000063895Add
BLAST

Proteomic databases

PRIDEiP24240.

Expressioni

Gene expression databases

GenevestigatoriP24240.

Interactioni

Protein-protein interaction databases

IntActiP24240. 5 interactions.
STRINGi511145.b2716.

Structurei

3D structure databases

ProteinModelPortaliP24240.
SMRiP24240. Positions 1-473.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088631.
KOiK01223.
OMAiRDLFNEC.
OrthoDBiEOG6F81PM.
PhylomeDBiP24240.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24240-1 [UniParc]FASTAAdd to Basket

« Hide

MSVFPESFLW GGALAANQSE GAFREGDKGL TTVDMIPHGE HRMAVKLGLE    50
KRFQLRDDEF YPSHEATDFY HRYKEDIALM AEMGFKVFRT SIAWSRLFPQ 100
GDEITPNQQG IAFYRSVFEE CKKYGIEPLV TLCHFDVPMH LVTEYGSWRN 150
RKLVEFFSRY ARTCFEAFDG LVKYWLTFNE INIMLHSPFS GAGLVFEEGE 200
NQDQVKYQAA HHQLVASALA TKIAHEVNPQ NQVGCMLAGG NFYPYSCKPE 250
DVWAALEKDR ENLFFIDVQA RGTYPAYSAR VFREKGVTIN KAPGDDEILK 300
NTVDFVSFSY YASRCASAEM NANNSSAANV VKSLRNPYLQ VSDWGWGIDP 350
LGLRITMNMM YDRYQKPLFL VENGLGAKDE FAANGEINDD YRISYLREHI 400
RAMGEAIADG IPLMGYTTWG CIDLVSASTG EMSKRYGFVF VDRDDAGNGT 450
LTRTRKKSFW WYKKVIASNG EDLE 474
Length:474
Mass (Da):53,935
Last modified:November 1, 1997 - v2
Checksum:i02ACE6BEBF211011
GO

Sequence cautioni

The sequence AAA69226.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti405 – 4062EA → GT in AAA16430. 1 Publication
Sequence conflicti428 – 4281S → C in AAA16430. 1 Publication
Sequence conflicti455 – 4562RK → HR in AAA16430. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M73326 Unassigned DNA. Translation: AAA16430.1.
U29579 Genomic DNA. Translation: AAA69226.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75758.1.
AP009048 Genomic DNA. Translation: BAE76793.1.
PIRiH65051.
RefSeqiNP_417196.1. NC_000913.3.
YP_490925.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC75758; AAC75758; b2716.
BAE76793; BAE76793; BAE76793.
GeneIDi12930434.
947460.
KEGGiecj:Y75_p2654.
eco:b2716.
PATRICi32120832. VBIEscCol129921_2808.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M73326 Unassigned DNA. Translation: AAA16430.1 .
U29579 Genomic DNA. Translation: AAA69226.1 . Different initiation.
U00096 Genomic DNA. Translation: AAC75758.1 .
AP009048 Genomic DNA. Translation: BAE76793.1 .
PIRi H65051.
RefSeqi NP_417196.1. NC_000913.3.
YP_490925.1. NC_007779.1.

3D structure databases

ProteinModelPortali P24240.
SMRi P24240. Positions 1-473.
ModBasei Search...

Protein-protein interaction databases

IntActi P24240. 5 interactions.
STRINGi 511145.b2716.

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEi P24240.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC75758 ; AAC75758 ; b2716 .
BAE76793 ; BAE76793 ; BAE76793 .
GeneIDi 12930434.
947460.
KEGGi ecj:Y75_p2654.
eco:b2716.
PATRICi 32120832. VBIEscCol129921_2808.

Organism-specific databases

EchoBASEi EB0083.
EcoGenei EG10085. ascB.

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088631.
KOi K01223.
OMAi RDLFNEC.
OrthoDBi EOG6F81PM.
PhylomeDBi P24240.

Enzyme and pathway databases

BioCyci EcoCyc:EG10085-MONOMER.
ECOL316407:JW2686-MONOMER.
MetaCyc:EG10085-MONOMER.

Miscellaneous databases

PROi P24240.

Gene expression databases

Genevestigatori P24240.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence, function, activation, and evolution of the cryptic asc operon of Escherichia coli K12."
    Hall B.G., Xu L.
    Mol. Biol. Evol. 9:688-706(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiASCB_ECOLI
AccessioniPrimary (citable) accession number: P24240
Secondary accession number(s): P78104, Q2MAB3, Q59375
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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