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Protein

ATP-dependent DNA helicase RecG

Gene

recG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Play a critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA). Has a role in constitutive stable DNA replication (cSDR) and R-loop formation. Is genetically synergistic to RadA and RuvABC (PubMed:12446634, PubMed:25484163).4 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi296 – 303ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • DNA duplex unwinding Source: EcoCyc
  • DNA recombination Source: UniProtKB-KW
  • DNA repair Source: UniProtKB-KW
  • response to radiation Source: EcoCyc
  • RNA secondary structure unwinding Source: GO_Central

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA recombination, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10829-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase RecG (EC:3.6.4.12)
Gene namesi
Name:recG
Synonyms:radC
Ordered Locus Names:b3652, JW3627
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10829. recG.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • Holliday junction helicase complex Source: EcoCyc

Pathology & Biotechi

Disruption phenotypei

Very slight sensitivity to ciprofloxacin (CFX), UV and azidothymidine (AZT) in single deletion mutants, but a radA-recG deletion is extremely sensitive to CFX and AZT, less so to UV (PubMed:12446634, PubMed:25484163). The SOS response is induced in this double mutant, indicating spontaneous DNA damage. AZT sensitivity is suppressed by further recA or recF deletions, suggesting AZT-induced DNA gaps are processed into lethal intermediates in a RecA-dependent fashion mediated by RecF (PubMed:25484163).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi411 – 693Missing in radC102; causes mild UV and X-ray sensitivity. 1 PublicationAdd BLAST283

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001021401 – 693ATP-dependent DNA helicase RecGAdd BLAST693

Proteomic databases

PaxDbiP24230.
PRIDEiP24230.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
aceFP069592EBI-556882,EBI-542707

Protein-protein interaction databases

BioGridi4262929. 148 interactors.
DIPiDIP-10653N.
IntActiP24230. 30 interactors.
MINTiMINT-1301344.
STRINGi316385.ECDH10B_3834.

Structurei

3D structure databases

ProteinModelPortaliP24230.
SMRiP24230.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini283 – 448Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST166
Domaini482 – 628Helicase C-terminalPROSITE-ProRule annotationAdd BLAST147

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi397 – 400DEQH box4

Sequence similaritiesi

Belongs to the helicase family. RecG subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105CB5. Bacteria.
COG1200. LUCA.
HOGENOMiHOG000036617.
InParanoidiP24230.
KOiK03655.
PhylomeDBiP24230.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
InterProiView protein in InterPro
IPR004609. ATP-dep_DNA_helicase_RecG.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR033454. RecG_wedge.
PfamiView protein in Pfam
PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF17191. RecG_wedge. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00643. recG. 1 hit.
PROSITEiView protein in PROSITE
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.

Sequencei

Sequence statusi: Complete.

P24230-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGRLLDAVP LSSLTGVGAA LSNKLAKINL HTVQDLLLHL PLRYEDRTHL
60 70 80 90 100
YPIGELLPGV YATVEGEVLN CNISFGGRRM MTCQISDGSG ILTMRFFNFS
110 120 130 140 150
AAMKNSLAAG RRVLAYGEAK RGKYGAEMIH PEYRVQGDLS TPELQETLTP
160 170 180 190 200
VYPTTEGVKQ ATLRKLTDQA LDLLDTCAIE ELLPPELSQG MMTLPEALRT
210 220 230 240 250
LHRPPPTLQL SDLETGQHPA QRRLILEELL AHNLSMLALR AGAQRFHAQP
260 270 280 290 300
LSANDTLKNK LLAALPFKPT GAQARVVAEI ERDMALDVPM MRLVQGDVGS
310 320 330 340 350
GKTLVAALAA LRAIAHGKQV ALMAPTELLA EQHANNFRNW FAPLGIEVGW
360 370 380 390 400
LAGKQKGKAR LAQQEAIASG QVQMIVGTHA IFQEQVQFNG LALVIIDEQH
410 420 430 440 450
RFGVHQRLAL WEKGQQQGFH PHQLIMTATP IPRTLAMTAY ADLDTSVIDE
460 470 480 490 500
LPPGRTPVTT VAIPDTRRTD IIDRVHHACI TEGRQAYWVC TLIEESELLE
510 520 530 540 550
AQAAEATWEE LKLALPELNV GLVHGRMKPA EKQAVMASFK QGELHLLVAT
560 570 580 590 600
TVIEVGVDVP NASLMIIENP ERLGLAQLHQ LRGRVGRGAV ASHCVLLYKT
610 620 630 640 650
PLSKTAQIRL QVLRDSNDGF VIAQKDLEIR GPGELLGTRQ TGNAEFKVAD
660 670 680 690
LLRDQAMIPE VQRLARHIHE RYPQQAKALI ERWMPETERY SNA
Length:693
Mass (Da):76,430
Last modified:March 1, 1992 - v1
Checksum:i7826143A8F4292A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59550 Genomic DNA. Translation: CAA42123.1.
M64367 Genomic DNA. Translation: AAA24513.1.
L10328 Genomic DNA. Translation: AAA62005.1.
U00096 Genomic DNA. Translation: AAC76676.1.
AP009048 Genomic DNA. Translation: BAE77641.1.
PIRiJH0265.
RefSeqiNP_418109.1. NC_000913.3.
WP_000678419.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76676; AAC76676; b3652.
BAE77641; BAE77641; BAE77641.
GeneIDi948162.
KEGGiecj:JW3627.
eco:b3652.
PATRICifig|511145.12.peg.3772.

Similar proteinsi

Entry informationi

Entry nameiRECG_ECOLI
AccessioniPrimary (citable) accession number: P24230
Secondary accession number(s): P76721, Q2M7W5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: October 25, 2017
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families