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Protein

ATP-dependent DNA helicase RecG

Gene

recG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Play a critical role in recombination and DNA repair. Helps process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with 3'- to 5'- polarity. Unwinds branched duplex DNA (Y-DNA). Has a role in constitutive stable DNA replication (cSDR) and R-loop formation. Is genetically synergistic to RadA and RuvABC (PubMed:12446634, PubMed:25484163).4 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi296 – 3038ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • DNA duplex unwinding Source: EcoCyc
  • DNA recombination Source: UniProtKB-KW
  • DNA repair Source: UniProtKB-KW
  • RNA secondary structure unwinding Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10829-MONOMER.
ECOL316407:JW3627-MONOMER.
RETL1328306-WGS:GSTH-2101-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase RecG (EC:3.6.4.12)
Gene namesi
Name:recG
Synonyms:radC
Ordered Locus Names:b3652, JW3627
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10829. recG.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • Holliday junction helicase complex Source: EcoCyc
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Very slight sensitivity to ciprofloxacin (CFX), UV and azidothymidine (AZT) in single deletion mutants, but a radA-recG deletion is extremely sensitive to CFX and AZT, less so to UV (PubMed:12446634, PubMed:25484163). The SOS response is induced in this double mutant, indicating spontaneous DNA damage. AZT sensitivity is suppressed by further recA or recF deletions, suggesting AZT-induced DNA gaps are processed into lethal intermediates in a RecA-dependent fashion mediated by RecF (PubMed:25484163).2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi411 – 693283Missing in radC102; causes mild UV and X-ray sensitivity. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 693693ATP-dependent DNA helicase RecGPRO_0000102140Add
BLAST

Proteomic databases

PaxDbiP24230.
PRIDEiP24230.

Interactioni

Protein-protein interaction databases

BioGridi4262929. 148 interactions.
DIPiDIP-10653N.
IntActiP24230. 28 interactions.
MINTiMINT-1301344.
STRINGi511145.b3652.

Structurei

3D structure databases

ProteinModelPortaliP24230.
SMRiP24230. Positions 264-645.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini283 – 448166Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini482 – 628147Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi397 – 4004DEQH box

Sequence similaritiesi

Belongs to the helicase family. RecG subfamily.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105CB5. Bacteria.
COG1200. LUCA.
HOGENOMiHOG000036617.
InParanoidiP24230.
KOiK03655.
OMAiSYYADLD.
PhylomeDBiP24230.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR004609. ATP-dep_DNA_helicase_RecG.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR033454. RecG_wedge.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF17191. RecG_wedge. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00643. recG. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24230-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGRLLDAVP LSSLTGVGAA LSNKLAKINL HTVQDLLLHL PLRYEDRTHL
60 70 80 90 100
YPIGELLPGV YATVEGEVLN CNISFGGRRM MTCQISDGSG ILTMRFFNFS
110 120 130 140 150
AAMKNSLAAG RRVLAYGEAK RGKYGAEMIH PEYRVQGDLS TPELQETLTP
160 170 180 190 200
VYPTTEGVKQ ATLRKLTDQA LDLLDTCAIE ELLPPELSQG MMTLPEALRT
210 220 230 240 250
LHRPPPTLQL SDLETGQHPA QRRLILEELL AHNLSMLALR AGAQRFHAQP
260 270 280 290 300
LSANDTLKNK LLAALPFKPT GAQARVVAEI ERDMALDVPM MRLVQGDVGS
310 320 330 340 350
GKTLVAALAA LRAIAHGKQV ALMAPTELLA EQHANNFRNW FAPLGIEVGW
360 370 380 390 400
LAGKQKGKAR LAQQEAIASG QVQMIVGTHA IFQEQVQFNG LALVIIDEQH
410 420 430 440 450
RFGVHQRLAL WEKGQQQGFH PHQLIMTATP IPRTLAMTAY ADLDTSVIDE
460 470 480 490 500
LPPGRTPVTT VAIPDTRRTD IIDRVHHACI TEGRQAYWVC TLIEESELLE
510 520 530 540 550
AQAAEATWEE LKLALPELNV GLVHGRMKPA EKQAVMASFK QGELHLLVAT
560 570 580 590 600
TVIEVGVDVP NASLMIIENP ERLGLAQLHQ LRGRVGRGAV ASHCVLLYKT
610 620 630 640 650
PLSKTAQIRL QVLRDSNDGF VIAQKDLEIR GPGELLGTRQ TGNAEFKVAD
660 670 680 690
LLRDQAMIPE VQRLARHIHE RYPQQAKALI ERWMPETERY SNA
Length:693
Mass (Da):76,430
Last modified:March 1, 1992 - v1
Checksum:i7826143A8F4292A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59550 Genomic DNA. Translation: CAA42123.1.
M64367 Genomic DNA. Translation: AAA24513.1.
L10328 Genomic DNA. Translation: AAA62005.1.
U00096 Genomic DNA. Translation: AAC76676.1.
AP009048 Genomic DNA. Translation: BAE77641.1.
PIRiJH0265.
RefSeqiNP_418109.1. NC_000913.3.
WP_000678419.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76676; AAC76676; b3652.
BAE77641; BAE77641; BAE77641.
GeneIDi948162.
KEGGiecj:JW3627.
eco:b3652.
PATRICi32122791. VBIEscCol129921_3772.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59550 Genomic DNA. Translation: CAA42123.1.
M64367 Genomic DNA. Translation: AAA24513.1.
L10328 Genomic DNA. Translation: AAA62005.1.
U00096 Genomic DNA. Translation: AAC76676.1.
AP009048 Genomic DNA. Translation: BAE77641.1.
PIRiJH0265.
RefSeqiNP_418109.1. NC_000913.3.
WP_000678419.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP24230.
SMRiP24230. Positions 264-645.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262929. 148 interactions.
DIPiDIP-10653N.
IntActiP24230. 28 interactions.
MINTiMINT-1301344.
STRINGi511145.b3652.

Proteomic databases

PaxDbiP24230.
PRIDEiP24230.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76676; AAC76676; b3652.
BAE77641; BAE77641; BAE77641.
GeneIDi948162.
KEGGiecj:JW3627.
eco:b3652.
PATRICi32122791. VBIEscCol129921_3772.

Organism-specific databases

EchoBASEiEB0822.
EcoGeneiEG10829. recG.

Phylogenomic databases

eggNOGiENOG4105CB5. Bacteria.
COG1200. LUCA.
HOGENOMiHOG000036617.
InParanoidiP24230.
KOiK03655.
OMAiSYYADLD.
PhylomeDBiP24230.

Enzyme and pathway databases

BioCyciEcoCyc:EG10829-MONOMER.
ECOL316407:JW3627-MONOMER.
RETL1328306-WGS:GSTH-2101-MONOMER.

Miscellaneous databases

PROiP24230.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR004609. ATP-dep_DNA_helicase_RecG.
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR033454. RecG_wedge.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
PF17191. RecG_wedge. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00643. recG. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRECG_ECOLI
AccessioniPrimary (citable) accession number: P24230
Secondary accession number(s): P76721, Q2M7W5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: September 7, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.