Reviewed,
UniProtKB/Swiss-Prot P24224 (ACPS_ECOLI)
Last modified
June 16, 2009.
Version 84.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Holo-[acyl-carrier-protein] synthase Short name=Holo-ACP synthase EC=2.7.8.7 Alternative name(s): 4'-phosphopantetheinyl transferase acpS | ||||||
| Gene names |
| ||||||
| Organism | Escherichia coli (strain K12) [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 83333 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia |
Protein attributes
| Sequence length | 126 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Transfers the 4'-phosphopantetheine moiety from coenzyme A to the 'Ser-36' of acyl-carrier-protein. HAMAP MF_00101 |
| Catalytic activity | CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein]. HAMAP MF_00101 |
| Cofactor | Magnesium. HAMAP MF_00101 |
| Subunit structure | Homodimer. HAMAP MF_00101 |
| Subcellular location | Cytoplasm Probable. |
| Sequence similarities | Belongs to the P-Pant transferase superfamily. AcpS family. |
| Mass spectrometry | Molecular mass is 13950 Da from positions 2 - 126. Determined by ESI. Ref.6 |
Ontologies
| Keywords | |
|---|---|
| Biological process | Fatty acid biosynthesis Lipid synthesis |
| Cellular component | Cytoplasm |
| Ligand | Magnesium Metal-binding |
| Molecular function | Transferase |
| Technical term | Complete proteome Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP macromolecule biosynthetic processInferred from electronic annotation. Source: InterPro |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | holo-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed Ref.6 | ||||||
| Chain | 2 – 126 | 125 | Holo-[acyl-carrier-protein] synthase HAMAP MF_00101 | PRO_0000175643 | |||||
Sites | |||||||||
| Metal binding | 9 | 1 | Magnesium By similarity | ||||||
| Metal binding | 58 | 1 | Magnesium By similarity | ||||||
Experimental info | |||||||||
| Mutagenesis | 5 | 1 | G → D: 5-fold reduction in catalytic activity. Ref.7 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Locating essential Escherichia coli genes by using mini-Tn10 transposons: the pdxJ operon." Takiff H.E., Baker T., Copeland T., Chen S.-M., Court D.L. J. Bacteriol. 174:1544-1553(1992) [PubMed: 1537799] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12. |
| [2] | "Suppression of insertions in the complex pdxJ operon of Escherichia coli K-12 by lon and other mutations." Lam H.-M., Tancula E., Dempsey W.B., Winkler M.E. J. Bacteriol. 174:1554-1567(1992) [PubMed: 1537800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12. |
| [3] | Nashimoto H., Saito N. Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12. |
| [4] | "The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076. |
| [5] | "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
| [6] | "Cloning, overproduction, and characterization of the Escherichia coli holo-acyl carrier protein synthase." Lambalot R.H., Walsh C.T. J. Biol. Chem. 270:24658-24661(1995) [PubMed: 7559576] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-26, CHARACTERIZATION, MASS SPECTROMETRY. Strain: K12. |
| [7] | "Holo-(acyl carrier protein) synthase and phosphopantetheinyl transfer in Escherichia coli." Flugel R.S., Hwangbo Y., Lambalot R.H., Cronan J.E. Jr., Walsh C.T. J. Biol. Chem. 275:959-968(2000) [PubMed: 10625633] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS, MUTAGENESIS OF GLY-5. Strain: K12 / W3110 / ATCC 27325 / DSM 5911. |
Cross-references
Sequence databases | |
|---|---|
| M76470 Genomic DNA. Translation: AAA21846.1. M74526 Genomic DNA. Translation: AAA24316.1. D64044 Genomic DNA. No translation available. U36841 Genomic DNA. Translation: AAA79825.1. U00096 Genomic DNA. Translation: AAC75616.1. AP009048 Genomic DNA. Translation: BAE76739.1. | |
| PIR | B42294. |
| RefSeq | AP_003149.1. NP_417058.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1F7L based on UniProtKB P96618. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 947037. |
| GenomeReviews | Gene locus JW2547 in contig AP009048_GR. Gene locus b2563 in contig U00096_GR. |
| KEGG | ecj:JW2547. eco:b2563. |
Organism-specific databases | |
| EchoBASE | EB0243. |
| EcoGene | EG10247. acpS. |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | P24224. |
| OMA | P24224. MGVVNLP. |
Enzyme and pathway databases | |
| BioCyc | EcoCyc:HOLO-ACP-SYNTH-MON. |
Family and domain databases | |
| HAMAP | MF_00101. [Tree] |
| InterPro | IPR008278. 4-PPantetheinyl_Trfase. IPR002582. ACPS. IPR004568. PPantethiene-prot_Trfase. [Graphical view] |
| Pfam | PF01648. ACPS. 1 hit. [Graphical view] |
| ProDom | PD004282. ACPS. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| TIGRFAMs | TIGR00516. acpS. 1 hit. TIGR00556. pantethn_trn. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | ACPS_ECOLI | ||||||||
| Accession | Primary (citable) accession number: P24224 Secondary accession number(s): Q2MAG7 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Escherichia coli Escherichia coli (strain K12): entries and cross-references to EcoGene |
| SIMILARITY comments Index of protein domains and families |

Clusters with


