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Protein

Holo-[acyl-carrier-protein] synthase

Gene

acpS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transfers the 4'-phosphopantetheine moiety from coenzyme A to the 'Ser-36' of acyl-carrier-protein.1 Publication

Catalytic activityi

CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein].UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9MagnesiumUniRule annotation1
Metal bindingi58MagnesiumUniRule annotation1

GO - Molecular functioni

  • holo-[acyl-carrier-protein] synthase activity Source: EcoliWiki
  • magnesium ion binding Source: UniProtKB-HAMAP
  • transferase activity Source: EcoliWiki

GO - Biological processi

  • fatty acid biosynthetic process Source: EcoliWiki
  • peptidyl-serine phosphopantetheinylation Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:HOLO-ACP-SYNTH-MONOMER.
ECOL316407:JW2547-MONOMER.
MetaCyc:HOLO-ACP-SYNTH-MONOMER.
BRENDAi2.7.8.7. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Holo-[acyl-carrier-protein] synthaseUniRule annotation (EC:2.7.8.7UniRule annotation)
Short name:
Holo-ACP synthaseUniRule annotation
Alternative name(s):
4'-phosphopantetheinyl transferase AcpSUniRule annotation
Gene namesi
Name:acpSUniRule annotation
Synonyms:dpj
Ordered Locus Names:b2563, JW2547
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10247. acpS.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi5G → D: 5-fold reduction in catalytic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001756432 – 126Holo-[acyl-carrier-protein] synthaseAdd BLAST125

Proteomic databases

PaxDbiP24224.
PRIDEiP24224.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4260598. 286 interactors.
DIPiDIP-9047N.
IntActiP24224. 13 interactors.
MINTiMINT-1256571.
STRINGi511145.b2563.

Structurei

3D structure databases

ProteinModelPortaliP24224.
SMRiP24224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the P-Pant transferase superfamily. AcpS family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105KJV. Bacteria.
COG0736. LUCA.
HOGENOMiHOG000014316.
InParanoidiP24224.
KOiK00997.
OMAiAQGVFWR.
PhylomeDBiP24224.

Family and domain databases

Gene3Di3.90.470.20. 1 hit.
HAMAPiMF_00101. AcpS. 1 hit.
InterProiIPR008278. 4-PPantetheinyl_Trfase_SF.
IPR002582. ACPS.
IPR004568. Ppantetheine-prot_Trfase_dom.
[Graphical view]
PfamiPF01648. ACPS. 1 hit.
[Graphical view]
ProDomiPD004282. PPantethiene-prot_Trfase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF56214. SSF56214. 1 hit.
TIGRFAMsiTIGR00516. acpS. 1 hit.
TIGR00556. pantethn_trn. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24224-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAILGLGTDI VEIARIEAVI ARSGDRLARR VLSDNEWAIW KTHHQPVRFL
60 70 80 90 100
AKRFAVKEAA AKAFGTGIRN GLAFNQFEVF NDELGKPRLR LWGEALKLAE
110 120
KLGVANMHVT LADERHYACA TVIIES
Length:126
Mass (Da):14,052
Last modified:January 23, 2007 - v3
Checksum:iA80628315E69731E
GO

Mass spectrometryi

Molecular mass is 13950 Da from positions 2 - 126. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76470 Genomic DNA. Translation: AAA21846.1.
M74526 Genomic DNA. Translation: AAA24316.1.
D64044 Genomic DNA. No translation available.
U36841 Genomic DNA. Translation: AAA79825.1.
U00096 Genomic DNA. Translation: AAC75616.1.
AP009048 Genomic DNA. Translation: BAE76739.1.
PIRiB42294.
RefSeqiNP_417058.1. NC_000913.3.
WP_000986023.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75616; AAC75616; b2563.
BAE76739; BAE76739; BAE76739.
GeneIDi947037.
KEGGiecj:JW2547.
eco:b2563.
PATRICi32120525. VBIEscCol129921_2665.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76470 Genomic DNA. Translation: AAA21846.1.
M74526 Genomic DNA. Translation: AAA24316.1.
D64044 Genomic DNA. No translation available.
U36841 Genomic DNA. Translation: AAA79825.1.
U00096 Genomic DNA. Translation: AAC75616.1.
AP009048 Genomic DNA. Translation: BAE76739.1.
PIRiB42294.
RefSeqiNP_417058.1. NC_000913.3.
WP_000986023.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP24224.
SMRiP24224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260598. 286 interactors.
DIPiDIP-9047N.
IntActiP24224. 13 interactors.
MINTiMINT-1256571.
STRINGi511145.b2563.

Proteomic databases

PaxDbiP24224.
PRIDEiP24224.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75616; AAC75616; b2563.
BAE76739; BAE76739; BAE76739.
GeneIDi947037.
KEGGiecj:JW2547.
eco:b2563.
PATRICi32120525. VBIEscCol129921_2665.

Organism-specific databases

EchoBASEiEB0243.
EcoGeneiEG10247. acpS.

Phylogenomic databases

eggNOGiENOG4105KJV. Bacteria.
COG0736. LUCA.
HOGENOMiHOG000014316.
InParanoidiP24224.
KOiK00997.
OMAiAQGVFWR.
PhylomeDBiP24224.

Enzyme and pathway databases

BioCyciEcoCyc:HOLO-ACP-SYNTH-MONOMER.
ECOL316407:JW2547-MONOMER.
MetaCyc:HOLO-ACP-SYNTH-MONOMER.
BRENDAi2.7.8.7. 2026.

Miscellaneous databases

PROiP24224.

Family and domain databases

Gene3Di3.90.470.20. 1 hit.
HAMAPiMF_00101. AcpS. 1 hit.
InterProiIPR008278. 4-PPantetheinyl_Trfase_SF.
IPR002582. ACPS.
IPR004568. Ppantetheine-prot_Trfase_dom.
[Graphical view]
PfamiPF01648. ACPS. 1 hit.
[Graphical view]
ProDomiPD004282. PPantethiene-prot_Trfase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF56214. SSF56214. 1 hit.
TIGRFAMsiTIGR00516. acpS. 1 hit.
TIGR00556. pantethn_trn. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiACPS_ECOLI
AccessioniPrimary (citable) accession number: P24224
Secondary accession number(s): Q2MAG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.