Reviewed, UniProtKB/Swiss-Prot P24224 (ACPS_ECOLI)

Names and origin

Protein names

Recommended name:
    Holo-[acyl-carrier-protein] synthase
      Short name=Holo-ACP synthase
    EC=2.7.8.7
Alternative name(s):
    4'-phosphopantetheinyl transferase acpS

Gene names

Name:	acpS
Synonyms:	dpj
Ordered Locus Names:	b2563, JW2547

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	126 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Transfers the 4'-phosphopantetheine moiety from coenzyme A to the 'Ser-36' of acyl-carrier-protein. HAMAP MF_00101
Catalytic activity	CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein]. HAMAP MF_00101
Cofactor	Magnesium. HAMAP MF_00101
Subunit structure	Homodimer. HAMAP MF_00101
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the P-Pant transferase superfamily. AcpS family.
Mass spectrometry	Molecular mass is 13950 Da from positions 2 - 126. Determined by ESI. Ref.6

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP macromolecule biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	holo-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.6
	Chain	2 – 126	125	Holo-[acyl-carrier-protein] synthase HAMAP MF_00101		PRO_0000175643
Sites
	Metal binding	9	1	Magnesium By similarity
	Metal binding	58	1	Magnesium By similarity
Experimental info
	Mutagenesis	5	1	G → D: 5-fold reduction in catalytic activity. Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P24224-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: A80628315E69731E	FASTA	126	14,052
	10 20 30 40 50 60 MAILGLGTDI VEIARIEAVI ARSGDRLARR VLSDNEWAIW KTHHQPVRFL AKRFAVKEAA 70 80 90 100 110 120 AKAFGTGIRN GLAFNQFEVF NDELGKPRLR LWGEALKLAE KLGVANMHVT LADERHYACA TVIIES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Locating essential Escherichia coli genes by using mini-Tn10 transposons: the pdxJ operon." Takiff H.E., Baker T., Copeland T., Chen S.-M., Court D.L. J. Bacteriol. 174:1544-1553(1992) [PubMed: 1537799] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Suppression of insertions in the complex pdxJ operon of Escherichia coli K-12 by lon and other mutations." Lam H.-M., Tancula E., Dempsey W.B., Winkler M.E. J. Bacteriol. 174:1554-1567(1992) [PubMed: 1537800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	Nashimoto H., Saito N. Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Cloning, overproduction, and characterization of the Escherichia coli holo-acyl carrier protein synthase." Lambalot R.H., Walsh C.T. J. Biol. Chem. 270:24658-24661(1995) [PubMed: 7559576] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-26, CHARACTERIZATION, MASS SPECTROMETRY. Strain: K12.
[7]	"Holo-(acyl carrier protein) synthase and phosphopantetheinyl transfer in Escherichia coli." Flugel R.S., Hwangbo Y., Lambalot R.H., Cronan J.E. Jr., Walsh C.T. J. Biol. Chem. 275:959-968(2000) [PubMed: 10625633] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS, MUTAGENESIS OF GLY-5. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M76470 Genomic DNA. Translation: AAA21846.1. M74526 Genomic DNA. Translation: AAA24316.1. D64044 Genomic DNA. No translation available. U36841 Genomic DNA. Translation: AAA79825.1. U00096 Genomic DNA. Translation: AAC75616.1. AP009048 Genomic DNA. Translation: BAE76739.1.
PIR	B42294.
RefSeq	AP_003149.1. NP_417058.1.
3D structure databases
HSSP	HSSP built from PDB template 1F7L based on UniProtKB P96618.
ModBase	Search...
Genome annotation databases
GeneID	947037.
GenomeReviews	Gene locus JW2547 in contig AP009048_GR. Gene locus b2563 in contig U00096_GR.
KEGG	ecj:JW2547. eco:b2563.
Organism-specific databases
EchoBASE	EB0243.
EcoGene	EG10247. acpS.
CMR	Search...
Phylogenomic databases
HOGENOM	P24224.
OMA	P24224. MGVVNLP.
Enzyme and pathway databases
BioCyc	EcoCyc:HOLO-ACP-SYNTH-MON.
Family and domain databases
HAMAP	MF_00101. [Tree]
InterPro	IPR008278. 4-PPantetheinyl_Trfase. IPR002582. ACPS. IPR004568. PPantethiene-prot_Trfase. [Graphical view]
Pfam	PF01648. ACPS. 1 hit. [Graphical view]
ProDom	PD004282. ACPS. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00516. acpS. 1 hit. TIGR00556. pantethn_trn. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACPS_ECOLI

Accession

Primary (citable) accession number: P24224
Secondary accession number(s): Q2MAG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)