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Protein

Flagellar hook-associated protein 2

Gene

fliD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the morphogenesis and for the elongation of the flagellar filament by facilitating polymerization of the flagellin monomers at the tip of growing filament. Forms a capping structure, which prevents flagellin subunits (transported through the central channel of the flagellum) from leaking out without polymerization at the distal end.

GO - Biological processi

  • bacterial-type flagellum-dependent cell motility Source: EcoCyc
  • cell adhesion Source: InterPro

Enzyme and pathway databases

BioCyciEcoCyc:EG10841-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flagellar hook-associated protein 2
Short name:
HAP2
Alternative name(s):
Filament cap protein
Flagellar cap protein
Gene namesi
Name:fliD
Synonyms:flaV, flbC
Ordered Locus Names:b1924, JW1909
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10841. fliD.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Bacterial flagellum, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001770172 – 468Flagellar hook-associated protein 2Add BLAST467

Proteomic databases

PaxDbiP24216.
PRIDEiP24216.

2D gel databases

SWISS-2DPAGEiP24216.

Interactioni

Subunit structurei

Homopentamer.Curated

Binary interactionsi

WithEntry#Exp.IntActNotes
fliTP0ABY24EBI-1126352,EBI-1114780

Protein-protein interaction databases

BioGridi4260370. 8 interactors.
IntActiP24216. 8 interactors.
STRINGi316385.ECDH10B_2065.

Structurei

Secondary structure

1468
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi45 – 64Combined sources20
Helixi67 – 71Combined sources5
Beta strandi73 – 78Combined sources6
Beta strandi82 – 86Combined sources5
Beta strandi93 – 101Combined sources9
Beta strandi107 – 112Combined sources6
Beta strandi117 – 119Combined sources3
Beta strandi126 – 133Combined sources8
Beta strandi139 – 143Combined sources5
Helixi145 – 147Combined sources3
Helixi150 – 160Combined sources11
Beta strandi163 – 172Combined sources10
Beta strandi175 – 184Combined sources10
Helixi187 – 189Combined sources3
Beta strandi191 – 197Combined sources7
Helixi199 – 205Combined sources7
Beta strandi211 – 213Combined sources3
Beta strandi215 – 220Combined sources6
Beta strandi225 – 229Combined sources5
Beta strandi232 – 237Combined sources6
Beta strandi239 – 245Combined sources7
Beta strandi248 – 252Combined sources5
Beta strandi261 – 266Combined sources6
Helixi269 – 293Combined sources25
Helixi316 – 330Combined sources15
Beta strandi335 – 337Combined sources3
Helixi341 – 344Combined sources4
Beta strandi346 – 348Combined sources3
Turni350 – 352Combined sources3
Beta strandi355 – 357Combined sources3
Helixi359 – 377Combined sources19
Turni378 – 380Combined sources3
Beta strandi381 – 383Combined sources3
Helixi386 – 398Combined sources13
Helixi403 – 410Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5H5VX-ray3.00A/B/C/D/E/F43-416[»]
5H5WX-ray2.15A/B71-267[»]
ProteinModelPortaliP24216.
SMRiP24216.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili411 – 439Sequence analysisAdd BLAST29

Sequence similaritiesi

Belongs to the FliD family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4105DTS. Bacteria.
COG1345. LUCA.
HOGENOMiHOG000254632.
InParanoidiP24216.
KOiK02407.
PhylomeDBiP24216.

Family and domain databases

InterProiView protein in InterPro
IPR010809. FliD_C.
IPR003481. FliD_N.
PfamiView protein in Pfam
PF07195. FliD_C. 1 hit.
PF02465. FliD_N. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24216-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASISSLGVG SGLDLSSILD SLTAAQKATL TPISNQQSSF TAKLSAYGTL
60 70 80 90 100
KSALTTFQTA NTALSKADLF SATSTTSSTT AFSATTAGNA IAGKYTISVT
110 120 130 140 150
HLAQAQTLTT RTTRDDTKTA IATSDSKLTI QQGGDKDPIT IDISAANSSL
160 170 180 190 200
SGIRDAINNA KAGVSASIIN VGNGEYRLSV TSNDTGLDNA MTLSVSGDDA
210 220 230 240 250
LQSFMGYDAS ASSNGMEVSV AAQNAQLTVN NVAIENSSNT ISDALENITL
260 270 280 290 300
NLNDVTTGNQ TLTITQDTSK AQTAIKDWVN AYNSLIDTFS SLTKYTAVDA
310 320 330 340 350
GADSQSSSNG ALLGDSTLRT IQTQLKSMLS NTVSSSSYKT LAQIGITTDP
360 370 380 390 400
SDGKLELDAD KLTAALKKDA SGVGALIVGD GKKTGITTTI GSNLTSWLST
410 420 430 440 450
TGIIKAATDG VSKTLNKLTK DYNAASDRID AQVARYKEQF TQLDVLMTSL
460
NSTSSYLTQQ FENNSNSK
Length:468
Mass (Da):48,456
Last modified:January 23, 2007 - v5
Checksum:i5C16E5B8DFBB8661
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti114R → T in AAA23790 (PubMed:1527488).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85240 Genomic DNA. Translation: AAA23790.1.
U00096 Genomic DNA. Translation: AAC74991.1.
AP009048 Genomic DNA. Translation: BAA15752.1.
X17440 Genomic DNA. Translation: CAA35487.1.
J01607 Genomic DNA. Translation: AAA92490.1.
PIRiA64956.
RefSeqiNP_416434.1. NC_000913.3.
WP_000146784.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74991; AAC74991; b1924.
BAA15752; BAA15752; BAA15752.
GeneIDi946428.
KEGGiecj:JW1909.
eco:b1924.
PATRICifig|1411691.4.peg.325.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiFLID_ECOLI
AccessioniPrimary (citable) accession number: P24216
Secondary accession number(s): P94745
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: July 5, 2017
This is version 132 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families