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Protein

Flagellar hook-associated protein 2

Gene

fliD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the morphogenesis and for the elongation of the flagellar filament by facilitating polymerization of the flagellin monomers at the tip of growing filament. Forms a capping structure, which prevents flagellin subunits (transported through the central channel of the flagellum) from leaking out without polymerization at the distal end.

GO - Biological processi

  • bacterial-type flagellum-dependent cell motility Source: EcoCyc
  • cell adhesion Source: InterPro
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:EG10841-MONOMER.
ECOL316407:JW1909-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Flagellar hook-associated protein 2
Short name:
HAP2
Alternative name(s):
Filament cap protein
Flagellar cap protein
Gene namesi
Name:fliD
Synonyms:flaV, flbC
Ordered Locus Names:b1924, JW1909
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10841. fliD.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Bacterial flagellum, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 468467Flagellar hook-associated protein 2PRO_0000177017Add
BLAST

Proteomic databases

PaxDbiP24216.
PRIDEiP24216.

2D gel databases

SWISS-2DPAGEP24216.

Interactioni

Subunit structurei

Homopentamer.Curated

Binary interactionsi

WithEntry#Exp.IntActNotes
fliTP0ABY24EBI-1126352,EBI-1114780

Protein-protein interaction databases

BioGridi4260370. 8 interactions.
IntActiP24216. 8 interactions.
STRINGi511145.b1924.

Structurei

3D structure databases

ProteinModelPortaliP24216.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili411 – 43929Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the FliD family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG4105DTS. Bacteria.
COG1345. LUCA.
HOGENOMiHOG000254632.
InParanoidiP24216.
KOiK02407.
OMAiQEAYTEV.
OrthoDBiEOG6GN6X6.
PhylomeDBiP24216.

Family and domain databases

InterProiIPR010809. FliD_C.
IPR003481. FliD_N.
[Graphical view]
PfamiPF07195. FliD_C. 1 hit.
PF02465. FliD_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24216-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASISSLGVG SGLDLSSILD SLTAAQKATL TPISNQQSSF TAKLSAYGTL
60 70 80 90 100
KSALTTFQTA NTALSKADLF SATSTTSSTT AFSATTAGNA IAGKYTISVT
110 120 130 140 150
HLAQAQTLTT RTTRDDTKTA IATSDSKLTI QQGGDKDPIT IDISAANSSL
160 170 180 190 200
SGIRDAINNA KAGVSASIIN VGNGEYRLSV TSNDTGLDNA MTLSVSGDDA
210 220 230 240 250
LQSFMGYDAS ASSNGMEVSV AAQNAQLTVN NVAIENSSNT ISDALENITL
260 270 280 290 300
NLNDVTTGNQ TLTITQDTSK AQTAIKDWVN AYNSLIDTFS SLTKYTAVDA
310 320 330 340 350
GADSQSSSNG ALLGDSTLRT IQTQLKSMLS NTVSSSSYKT LAQIGITTDP
360 370 380 390 400
SDGKLELDAD KLTAALKKDA SGVGALIVGD GKKTGITTTI GSNLTSWLST
410 420 430 440 450
TGIIKAATDG VSKTLNKLTK DYNAASDRID AQVARYKEQF TQLDVLMTSL
460
NSTSSYLTQQ FENNSNSK
Length:468
Mass (Da):48,456
Last modified:January 23, 2007 - v5
Checksum:i5C16E5B8DFBB8661
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1141R → T in AAA23790 (PubMed:1527488).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85240 Genomic DNA. Translation: AAA23790.1.
U00096 Genomic DNA. Translation: AAC74991.1.
AP009048 Genomic DNA. Translation: BAA15752.1.
X17440 Genomic DNA. Translation: CAA35487.1.
J01607 Genomic DNA. Translation: AAA92490.1.
PIRiA64956.
RefSeqiNP_416434.1. NC_000913.3.
WP_000146784.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74991; AAC74991; b1924.
BAA15752; BAA15752; BAA15752.
GeneIDi946428.
KEGGiecj:JW1909.
eco:b1924.
PATRICi32119183. VBIEscCol129921_2006.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M85240 Genomic DNA. Translation: AAA23790.1.
U00096 Genomic DNA. Translation: AAC74991.1.
AP009048 Genomic DNA. Translation: BAA15752.1.
X17440 Genomic DNA. Translation: CAA35487.1.
J01607 Genomic DNA. Translation: AAA92490.1.
PIRiA64956.
RefSeqiNP_416434.1. NC_000913.3.
WP_000146784.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP24216.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260370. 8 interactions.
IntActiP24216. 8 interactions.
STRINGi511145.b1924.

2D gel databases

SWISS-2DPAGEP24216.

Proteomic databases

PaxDbiP24216.
PRIDEiP24216.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74991; AAC74991; b1924.
BAA15752; BAA15752; BAA15752.
GeneIDi946428.
KEGGiecj:JW1909.
eco:b1924.
PATRICi32119183. VBIEscCol129921_2006.

Organism-specific databases

EchoBASEiEB0834.
EcoGeneiEG10841. fliD.

Phylogenomic databases

eggNOGiENOG4105DTS. Bacteria.
COG1345. LUCA.
HOGENOMiHOG000254632.
InParanoidiP24216.
KOiK02407.
OMAiQEAYTEV.
OrthoDBiEOG6GN6X6.
PhylomeDBiP24216.

Enzyme and pathway databases

BioCyciEcoCyc:EG10841-MONOMER.
ECOL316407:JW1909-MONOMER.

Miscellaneous databases

PROiP24216.

Family and domain databases

InterProiIPR010809. FliD_C.
IPR003481. FliD_N.
[Graphical view]
PfamiPF07195. FliD_C. 1 hit.
PF02465. FliD_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Subdivision of flagellar region III of the Escherichia coli and Salmonella typhimurium chromosomes and identification of two additional flagellar genes."
    Kawagishi I., Mueller V., Williams A.W., Irikura V.M., Macnab R.M.
    J. Gen. Microbiol. 138:1051-1065(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: JA11.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Isolation and characterization of Escherichia coli hag operator mutants whose hag48 expression has become repressible by a Salmonella H1 repressor."
    Hanafusa T., Sakai A., Tominaga A., Enomoto M.
    Mol. Gen. Genet. 216:44-50(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-153.
    Strain: K12.
  6. "DNA sequence adjacent to flagellar genes and evolution of flagellar-phase variation."
    Szekely E., Simon M.
    J. Bacteriol. 155:74-81(1983) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.

Entry informationi

Entry nameiFLID_ECOLI
AccessioniPrimary (citable) accession number: P24216
Secondary accession number(s): P94745
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: January 20, 2016
This is version 125 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.