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Reviewed, UniProtKB/Swiss-Prot P24215 (UXUA_ECOLI)

Last modified June 15, 2010. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
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Names and originHide

Protein namesRecommended name:
Mannonate dehydratase
EC=4.2.1.8
Alternative name(s):
D-mannonate hydrolase
Gene names
Name:uxuA
Ordered Locus Names:b4322, JW4285
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia
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Protein attributesHide

Sequence length394 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.
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General annotation (Comments)Hide

Catalytic activity

D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O. HAMAP MF_00106

Pathway

Carbohydrate metabolism; pentose and glucuronate interconversion. HAMAP MF_00106

Induction

By fructuronate. Its expression is subjected to catabolite repression by glucose. HAMAP MF_00106

Sequence similarities

Belongs to the mannonate dehydratase family.

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OntologiesHide

Keywords
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processD-glucuronate catabolic process

Inferred from mutant phenotype. Source: UniProtKB

   Molecular functionferrous iron binding

Inferred from direct assay. Source: UniProtKB

mannonate dehydratase activity

Inferred from electronic annotation. Source: HAMAP

protein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...
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Binary interactionsHide

With

Entry

#Exp.

IntAct

Notes

nadEP188431EBI-1130634,EBI-548960
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Sequence annotation (Features)Hide

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 394394Mannonate dehydratase HAMAP MF_00106
PRO_0000170670

Natural variations

Natural variant521I → V in strain: MT78.
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SequencesHide

Sequence LengthMass (Da)Tools
P24215-1 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 0716A50BD6436679

FASTA39444,838
        10         20         30         40         50         60 
MEQTWRWYGP NDPVSLADVR QAGATGVVTA LHHIPNGEVW SVEEILKRKA IIEDAGLVWS 

        70         80         90        100        110        120 
VVESVPIHED IKTHTGNYEQ WIANYQQTLR NLAQCGIRTV CYNFMPVLDW TRTDLEYVLP 

       130        140        150        160        170        180 
DGSKALRFDQ IEFAAFEMHI LKRPGAEADY TEEEIAQAAE RFATMSDEDK ARLTRNIIAG 

       190        200        210        220        230        240 
LPGAEEGYTL DQFRKHLELY KDIDKAKLRE NFAVFLKAII PVAEEVGVRM AVHPDDPPRP 

       250        260        270        280        290        300 
ILGLPRIVST IEDMQWMVDT VNSMANGFTM CTGSYGVRAD NDLVDMIKQF GPRIYFTHLR 

       310        320        330        340        350        360 
STMREDNPKT FHEAAHLNGD VDMYEVVKAI VEEEHRRKAE GKEDLIPMRP DHGHQMLDDL 

       370        380        390 
KKKTNPGYSA IGRLKGLAEV RGVELAIQRA FFSR

« Hide

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ReferencesHide

« Hide 'large scale' references
[1]Mizobuchi K.
Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Colonization ability and pathogenic properties of a fim- mutant of an avian strain of Escherichia coli."
Marc D., Arne P., Bree A., Dho-Moulin M.
Res. Microbiol. 149:473-485(1998) [PubMed: 9766199] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-128.
Strain: O2:K1:H+ / MT78.
[6]"The regulatory region of the uxuAB operon in Escherichia coli K12."
Blanco C., Ritzenthaler P., Kolb A.
Mol. Gen. Genet. 202:112-119(1986) [PubMed: 3083215] [Abstract]
Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
Strain: K12.
[7]"The gntP gene of Escherichia coli involved in gluconate uptake."
Klemm P., Tong S., Nielsen H., Conway T.
J. Bacteriol. 178:61-67(1996) [PubMed: 8550444] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.
Strain: K12.
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Cross-referencesHide

Sequence databases

EMBL
GenBank
DDBJ		D13329 Genomic DNA. Translation: BAA02590.1.
U14003 Genomic DNA. Translation: AAA97218.1.
U00096 Genomic DNA. Translation: AAC77278.1.
AP009048 Genomic DNA. Translation: BAE78315.1.
AJ225176 Genomic DNA. Translation: CAA12424.1.
X03411 Genomic DNA. Translation: CAA27147.1. Different termination.
X91735 Genomic DNA. Translation: CAA62860.1.
PIRI57745.
S56547.
RefSeqAP_004814.1.
NP_418742.1.

3D structure databases

SMRP24215. Positions 1-389.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-11108N.
IntActP24215. 2 interactions.
STRINGP24215.

Genome annotation databases

EnsemblBacteriaEBESCT00000000542; EBESCP00000000542; EBESCG00000000456.
EBESCT00000016047; EBESCP00000015338; EBESCG00000015107.
GeneID947082.
GenomeReviewsGene locus JW4285 in contig AP009048_GR.
Gene locus b4322 in contig U00096_GR.
KEGGecj:JW4285.
eco:b4322.

Organism-specific databases

EchoBASEEB1059.
EcoGeneEG11066. uxuA.
CMRSearch...

Phylogenomic databases

eggNOGCOG1312.
HOGENOMHBG585927.
OMAVHEEIKT.
ProtClustDBPRK03906.

Enzyme and pathway databases

BioCycEcoCyc:MANNONDEHYDRAT-MONOMER.
ECOL168927:B4322-MONOMER.
MetaCyc:MANNONDEHYDRAT-MONOMER.

Gene expression databases

GenevestigatorP24215.

Family and domain databases

HAMAPMF_00106. UxuA.
[Tree]
InterProIPR004628. Man_deHydtase.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
Gene3DG3DSA:3.20.20.150. Xyl_isomerase-like_TIM-brl. 1 hit.
PfamPF03786. UxuA. 1 hit.
[Graphical view]
PIRSFPIRSF016049. Man_dehyd. 1 hit.
SUPFAMSSF51658. Xyl_isomerase-like_TIM-brl. 1 hit.
TIGRFAMsTIGR00695. uxuA. 1 hit.
ProtoNetSearch...
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Entry informationHide

Entry nameUXUA_ECOLI
AccessionPrimary (citable) accession number: P24215
Secondary accession number(s): O87739, Q2M5Z1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: February 1, 1995
Last modified: June 15, 2010
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)
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Relevant documentsHide

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents