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Protein

Mannonate dehydratase

Gene

uxuA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the dehydration of D-mannonate.1 Publication

Catalytic activityi

D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O.1 Publication

Cofactori

Fe2+1 Publication, Mn2+1 PublicationNote: Mn2+ can substitute for iron, but in higher concentrations. Cannot use Fe3+, Ni2+ or Mg2+.1 Publication

Enzyme regulationi

Is inhibited by high concentrations of Fe2+ (> 2 mM), and by EDTA or other iron chelators in vitro.1 Publication

pH dependencei

Optimum pH is about 7.5. The activity at pH 6.5 and 8.6 is only about 20% of the maximal achievable activity at optimum pH.1 Publication

Pathwayi: pentose and glucuronate interconversion

This protein is involved in the pathway pentose and glucuronate interconversion, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway pentose and glucuronate interconversion and in Carbohydrate metabolism.

GO - Molecular functioni

  • ferrous iron binding Source: EcoCyc
  • manganese ion binding Source: EcoCyc
  • mannonate dehydratase activity Source: CACAO

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • D-glucuronate catabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Iron, Manganese

Enzyme and pathway databases

BioCyciEcoCyc:MANNONDEHYDRAT-MONOMER.
ECOL316407:JW4285-MONOMER.
MetaCyc:MANNONDEHYDRAT-MONOMER.
UniPathwayiUPA00246.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannonate dehydratase (EC:4.2.1.8)
Alternative name(s):
D-mannonate hydro-lyase
Gene namesi
Name:uxuA
Ordered Locus Names:b4322, JW4285
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11066. uxuA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001706701 – 394Mannonate dehydrataseAdd BLAST394

Proteomic databases

EPDiP24215.
PaxDbiP24215.
PRIDEiP24215.

Expressioni

Inductioni

By fructuronate. Its expression is subjected to catabolite repression by glucose.

Interactioni

Protein-protein interaction databases

BioGridi4261003. 10 interactors.
DIPiDIP-11108N.
IntActiP24215. 2 interactors.
STRINGi511145.b4322.

Structurei

Secondary structure

1394
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 6Combined sources4
Helixi16 – 22Combined sources7
Beta strandi26 – 29Combined sources4
Helixi42 – 54Combined sources13
Beta strandi58 – 63Combined sources6
Helixi69 – 73Combined sources5
Helixi78 – 94Combined sources17
Beta strandi99 – 102Combined sources4
Beta strandi113 – 118Combined sources6
Beta strandi124 – 128Combined sources5
Helixi130 – 139Combined sources10
Helixi146 – 149Combined sources4
Helixi152 – 163Combined sources12
Helixi167 – 178Combined sources12
Helixi190 – 198Combined sources9
Turni199 – 202Combined sources4
Helixi205 – 226Combined sources22
Beta strandi229 – 232Combined sources4
Beta strandi236 – 239Combined sources4
Beta strandi248 – 250Combined sources3
Helixi251 – 260Combined sources10
Beta strandi266 – 271Combined sources6
Helixi272 – 275Combined sources4
Helixi283 – 290Combined sources8
Helixi291 – 293Combined sources3
Beta strandi294 – 298Combined sources5
Beta strandi302 – 304Combined sources3
Beta strandi311 – 313Combined sources3
Beta strandi318 – 321Combined sources4
Helixi323 – 339Combined sources17
Helixi357 – 361Combined sources5
Helixi371 – 391Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EACX-ray2.30A/B/C/D1-394[»]
4EAYX-ray2.35A/B/C/D1-394[»]
ProteinModelPortaliP24215.
SMRiP24215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the mannonate dehydratase family.Curated

Phylogenomic databases

eggNOGiENOG4105CHZ. Bacteria.
COG1312. LUCA.
HOGENOMiHOG000195381.
InParanoidiP24215.
KOiK01686.
OMAiEGDVDMY.
PhylomeDBiP24215.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00106. UxuA. 1 hit.
InterProiIPR004628. Man_deHydtase.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PfamiPF03786. UxuA. 1 hit.
[Graphical view]
PIRSFiPIRSF016049. Man_dehyd. 1 hit.
SUPFAMiSSF51658. SSF51658. 2 hits.
TIGRFAMsiTIGR00695. uxuA. 1 hit.

Sequencei

Sequence statusi: Complete.

P24215-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQTWRWYGP NDPVSLADVR QAGATGVVTA LHHIPNGEVW SVEEILKRKA
60 70 80 90 100
IIEDAGLVWS VVESVPIHED IKTHTGNYEQ WIANYQQTLR NLAQCGIRTV
110 120 130 140 150
CYNFMPVLDW TRTDLEYVLP DGSKALRFDQ IEFAAFEMHI LKRPGAEADY
160 170 180 190 200
TEEEIAQAAE RFATMSDEDK ARLTRNIIAG LPGAEEGYTL DQFRKHLELY
210 220 230 240 250
KDIDKAKLRE NFAVFLKAII PVAEEVGVRM AVHPDDPPRP ILGLPRIVST
260 270 280 290 300
IEDMQWMVDT VNSMANGFTM CTGSYGVRAD NDLVDMIKQF GPRIYFTHLR
310 320 330 340 350
STMREDNPKT FHEAAHLNGD VDMYEVVKAI VEEEHRRKAE GKEDLIPMRP
360 370 380 390
DHGHQMLDDL KKKTNPGYSA IGRLKGLAEV RGVELAIQRA FFSR
Length:394
Mass (Da):44,838
Last modified:February 1, 1995 - v2
Checksum:i0716A50BD6436679
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti52I → V in strain: MT78. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13329 Genomic DNA. Translation: BAA02590.1.
U14003 Genomic DNA. Translation: AAA97218.1.
U00096 Genomic DNA. Translation: AAC77278.1.
AP009048 Genomic DNA. Translation: BAE78315.1.
AJ225176 Genomic DNA. Translation: CAA12424.1.
X03411 Genomic DNA. Translation: CAA27147.1. Different termination.
X91735 Genomic DNA. Translation: CAA62860.1.
PIRiI57745.
S56547.
RefSeqiNP_418742.1. NC_000913.3.
WP_000438562.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77278; AAC77278; b4322.
BAE78315; BAE78315; BAE78315.
GeneIDi947082.
KEGGiecj:JW4285.
eco:b4322.
PATRICi32124240. VBIEscCol129921_4463.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13329 Genomic DNA. Translation: BAA02590.1.
U14003 Genomic DNA. Translation: AAA97218.1.
U00096 Genomic DNA. Translation: AAC77278.1.
AP009048 Genomic DNA. Translation: BAE78315.1.
AJ225176 Genomic DNA. Translation: CAA12424.1.
X03411 Genomic DNA. Translation: CAA27147.1. Different termination.
X91735 Genomic DNA. Translation: CAA62860.1.
PIRiI57745.
S56547.
RefSeqiNP_418742.1. NC_000913.3.
WP_000438562.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EACX-ray2.30A/B/C/D1-394[»]
4EAYX-ray2.35A/B/C/D1-394[»]
ProteinModelPortaliP24215.
SMRiP24215.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261003. 10 interactors.
DIPiDIP-11108N.
IntActiP24215. 2 interactors.
STRINGi511145.b4322.

Proteomic databases

EPDiP24215.
PaxDbiP24215.
PRIDEiP24215.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77278; AAC77278; b4322.
BAE78315; BAE78315; BAE78315.
GeneIDi947082.
KEGGiecj:JW4285.
eco:b4322.
PATRICi32124240. VBIEscCol129921_4463.

Organism-specific databases

EchoBASEiEB1059.
EcoGeneiEG11066. uxuA.

Phylogenomic databases

eggNOGiENOG4105CHZ. Bacteria.
COG1312. LUCA.
HOGENOMiHOG000195381.
InParanoidiP24215.
KOiK01686.
OMAiEGDVDMY.
PhylomeDBiP24215.

Enzyme and pathway databases

UniPathwayiUPA00246.
BioCyciEcoCyc:MANNONDEHYDRAT-MONOMER.
ECOL316407:JW4285-MONOMER.
MetaCyc:MANNONDEHYDRAT-MONOMER.

Miscellaneous databases

PROiP24215.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00106. UxuA. 1 hit.
InterProiIPR004628. Man_deHydtase.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PfamiPF03786. UxuA. 1 hit.
[Graphical view]
PIRSFiPIRSF016049. Man_dehyd. 1 hit.
SUPFAMiSSF51658. SSF51658. 2 hits.
TIGRFAMsiTIGR00695. uxuA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUXUA_ECOLI
AccessioniPrimary (citable) accession number: P24215
Secondary accession number(s): O87739, Q2M5Z1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.