P24215 (UXUA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannonate dehydratase
EC=4.2.1.8

Alternative name(s):
D-mannonate hydrolase

Gene names

Name:	uxuA
Ordered Locus Names:	b4322, JW4285

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O. HAMAP MF_00106
Pathway	Carbohydrate metabolism; pentose and glucuronate interconversion. HAMAP MF_00106
Induction	By fructuronate. Its expression is subjected to catabolite repression by glucose. HAMAP MF_00106
Sequence similarities	Belongs to the mannonate dehydratase family.

Ontologies

Keywords
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	D-glucuronate catabolic process Inferred from mutant phenotype. Source: EcoCyc
Molecular function	ferrous iron binding Inferred from direct assay. Source: EcoCyc mannonate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
nadE	P18843	1	EBI-1130634,EBI-548960

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Mannonate dehydratase HAMAP MF_00106		PRO_0000170670
Natural variations
	Natural variant	52	1	I → V in strain: MT78.

Sequences

	Sequence		Length	Mass (Da)
	P24215 [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 0716A50BD6436679	FASTA	394	44,838
	10 20 30 40 50 60 MEQTWRWYGP NDPVSLADVR QAGATGVVTA LHHIPNGEVW SVEEILKRKA IIEDAGLVWS 70 80 90 100 110 120 VVESVPIHED IKTHTGNYEQ WIANYQQTLR NLAQCGIRTV CYNFMPVLDW TRTDLEYVLP 130 140 150 160 170 180 DGSKALRFDQ IEFAAFEMHI LKRPGAEADY TEEEIAQAAE RFATMSDEDK ARLTRNIIAG 190 200 210 220 230 240 LPGAEEGYTL DQFRKHLELY KDIDKAKLRE NFAVFLKAII PVAEEVGVRM AVHPDDPPRP 250 260 270 280 290 300 ILGLPRIVST IEDMQWMVDT VNSMANGFTM CTGSYGVRAD NDLVDMIKQF GPRIYFTHLR 310 320 330 340 350 360 STMREDNPKT FHEAAHLNGD VDMYEVVKAI VEEEHRRKAE GKEDLIPMRP DHGHQMLDDL 370 380 390 KKKTNPGYSA IGRLKGLAEV RGVELAIQRA FFSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Mizobuchi K. Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Colonization ability and pathogenic properties of a fim- mutant of an avian strain of Escherichia coli." Marc D., Arne P., Bree A., Dho-Moulin M. Res. Microbiol. 149:473-485(1998) [PubMed: 9766199] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-128. Strain: O2:K1:H+ / MT78.
[6]	"The regulatory region of the uxuAB operon in Escherichia coli K12." Blanco C., Ritzenthaler P., Kolb A. Mol. Gen. Genet. 202:112-119(1986) [PubMed: 3083215] [Abstract] Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11. Strain: K12.
[7]	"The gntP gene of Escherichia coli involved in gluconate uptake." Klemm P., Tong S., Nielsen H., Conway T. J. Bacteriol. 178:61-67(1996) [PubMed: 8550444] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10. Strain: K12.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13329 Genomic DNA. Translation: BAA02590.1. U14003 Genomic DNA. Translation: AAA97218.1. U00096 Genomic DNA. Translation: AAC77278.1. AP009048 Genomic DNA. Translation: BAE78315.1. AJ225176 Genomic DNA. Translation: CAA12424.1. X03411 Genomic DNA. Translation: CAA27147.1. Different termination. X91735 Genomic DNA. Translation: CAA62860.1.
PIR	I57745. S56547.
RefSeq	NP_418742.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P24215.
SMR	P24215. Positions 1-389.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-11108N.
IntAct	P24215. 2 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000542; EBESCP00000000542; EBESCG00000000456. EBESCT00000016047; EBESCP00000015338; EBESCG00000015107.
GeneID	947082.
GenomeReviews	Gene locus JW4285 in contig AP009048_GR. Gene locus b4322 in contig U00096_GR.
KEGG	ecj:JW4285. eco:b4322.
PATRIC	32124240. VBIEscCol129921_4463.
Organism-specific databases
EchoBASE	EB1059.
EcoGene	EG11066. uxuA.
Phylogenomic databases
eggNOG	COG1312.
GeneTree	EBGT00050000011670.
HOGENOM	HBG585927.
OMA	VHEEIKT.
PhylomeDB	P24215.
ProtClustDB	PRK03906.
Enzyme and pathway databases
BioCyc	EcoCyc:MANNONDEHYDRAT-MONOMER. MetaCyc:MANNONDEHYDRAT-MONOMER.
Gene expression databases
Genevestigator	P24215.
Family and domain databases
HAMAP	MF_00106. UxuA. [Tree]
InterPro	IPR004628. Man_deHydtase. IPR013022. Xyl_isomerase-like_TIM-brl. [Graphical view]
Gene3D	G3DSA:3.20.20.150. Xyl_isomerase-like_TIM-brl. 1 hit.
KO	K01686.
Pfam	PF03786. UxuA. 1 hit. [Graphical view]
PIRSF	PIRSF016049. Man_dehyd. 1 hit.
SUPFAM	SSF51658. Xyl_isomerase-like_TIM-brl. 1 hit.
TIGRFAMs	TIGR00695. UxuA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UXUA_ECOLI

Accession

Primary (citable) accession number: P24215
Secondary accession number(s): O87739, Q2M5Z1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	February 1, 1995
Last modified:	January 25, 2012
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program