Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P24215

- UXUA_ECOLI

UniProt

P24215 - UXUA_ECOLI

Protein

Mannonate dehydratase

Gene

uxuA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (01 Feb 1995)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the dehydration of D-mannonate.1 Publication

    Catalytic activityi

    D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O.1 Publication

    Cofactori

    Fe2+ ion. Mn2+ can substitute for iron, but in higher concentrations. Cannot use Fe3+, Ni2+ or Mg2+.1 Publication

    Enzyme regulationi

    Is inhibited by high concentrations of Fe2+ (> 2 mM), and by EDTA or other iron chelators in vitro.1 Publication

    pH dependencei

    Optimum pH is about 7.5. The activity at pH 6.5 and 8.6 is only about 20% of the maximal achievable activity at optimum pH.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. ferrous iron binding Source: EcoCyc
    2. manganese ion binding Source: EcoCyc
    3. mannonate dehydratase activity Source: CACAO

    GO - Biological processi

    1. cellular response to DNA damage stimulus Source: EcoliWiki
    2. D-glucuronate catabolic process Source: EcoCyc

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Iron, Manganese

    Enzyme and pathway databases

    BioCyciEcoCyc:MANNONDEHYDRAT-MONOMER.
    ECOL316407:JW4285-MONOMER.
    MetaCyc:MANNONDEHYDRAT-MONOMER.
    UniPathwayiUPA00246.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannonate dehydratase (EC:4.2.1.8)
    Alternative name(s):
    D-mannonate hydro-lyase
    Gene namesi
    Name:uxuA
    Ordered Locus Names:b4322, JW4285
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11066. uxuA.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 394394Mannonate dehydratasePRO_0000170670Add
    BLAST

    Proteomic databases

    PaxDbiP24215.
    PRIDEiP24215.

    Expressioni

    Inductioni

    By fructuronate. Its expression is subjected to catabolite repression by glucose.

    Gene expression databases

    GenevestigatoriP24215.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    nadEP188431EBI-1130634,EBI-548960

    Protein-protein interaction databases

    DIPiDIP-11108N.
    IntActiP24215. 2 interactions.
    STRINGi511145.b4322.

    Structurei

    Secondary structure

    1
    394
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 64
    Helixi16 – 227
    Beta strandi26 – 294
    Helixi42 – 5413
    Beta strandi58 – 636
    Helixi69 – 735
    Helixi78 – 9417
    Beta strandi99 – 1024
    Beta strandi113 – 1186
    Beta strandi124 – 1285
    Helixi130 – 13910
    Helixi146 – 1494
    Helixi152 – 16312
    Helixi167 – 17812
    Helixi190 – 1989
    Turni199 – 2024
    Helixi205 – 22622
    Beta strandi229 – 2324
    Beta strandi236 – 2394
    Beta strandi248 – 2503
    Helixi251 – 26010
    Beta strandi266 – 2716
    Helixi272 – 2754
    Helixi283 – 2908
    Helixi291 – 2933
    Beta strandi294 – 2985
    Beta strandi302 – 3043
    Beta strandi311 – 3133
    Beta strandi318 – 3214
    Helixi323 – 33917
    Helixi357 – 3615
    Helixi371 – 39121

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4EACX-ray2.30A/B/C/D1-394[»]
    4EAYX-ray2.35A/B/C/D1-394[»]
    ProteinModelPortaliP24215.
    SMRiP24215. Positions 1-394.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the mannonate dehydratase family.Curated

    Phylogenomic databases

    eggNOGiCOG1312.
    HOGENOMiHOG000195381.
    KOiK01686.
    OMAiIPNGQVW.
    OrthoDBiEOG6ZSP6Z.
    PhylomeDBiP24215.

    Family and domain databases

    Gene3Di3.20.20.150. 1 hit.
    HAMAPiMF_00106. UxuA.
    InterProiIPR004628. Man_deHydtase.
    IPR013022. Xyl_isomerase-like_TIM-brl.
    [Graphical view]
    PfamiPF03786. UxuA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016049. Man_dehyd. 1 hit.
    SUPFAMiSSF51658. SSF51658. 2 hits.
    TIGRFAMsiTIGR00695. uxuA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P24215-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEQTWRWYGP NDPVSLADVR QAGATGVVTA LHHIPNGEVW SVEEILKRKA    50
    IIEDAGLVWS VVESVPIHED IKTHTGNYEQ WIANYQQTLR NLAQCGIRTV 100
    CYNFMPVLDW TRTDLEYVLP DGSKALRFDQ IEFAAFEMHI LKRPGAEADY 150
    TEEEIAQAAE RFATMSDEDK ARLTRNIIAG LPGAEEGYTL DQFRKHLELY 200
    KDIDKAKLRE NFAVFLKAII PVAEEVGVRM AVHPDDPPRP ILGLPRIVST 250
    IEDMQWMVDT VNSMANGFTM CTGSYGVRAD NDLVDMIKQF GPRIYFTHLR 300
    STMREDNPKT FHEAAHLNGD VDMYEVVKAI VEEEHRRKAE GKEDLIPMRP 350
    DHGHQMLDDL KKKTNPGYSA IGRLKGLAEV RGVELAIQRA FFSR 394
    Length:394
    Mass (Da):44,838
    Last modified:February 1, 1995 - v2
    Checksum:i0716A50BD6436679
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti52 – 521I → V in strain: MT78.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13329 Genomic DNA. Translation: BAA02590.1.
    U14003 Genomic DNA. Translation: AAA97218.1.
    U00096 Genomic DNA. Translation: AAC77278.1.
    AP009048 Genomic DNA. Translation: BAE78315.1.
    AJ225176 Genomic DNA. Translation: CAA12424.1.
    X03411 Genomic DNA. Translation: CAA27147.1. Different termination.
    X91735 Genomic DNA. Translation: CAA62860.1.
    PIRiI57745.
    S56547.
    RefSeqiNP_418742.1. NC_000913.3.
    YP_492456.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77278; AAC77278; b4322.
    BAE78315; BAE78315; BAE78315.
    GeneIDi12932009.
    947082.
    KEGGiecj:Y75_p4208.
    eco:b4322.
    PATRICi32124240. VBIEscCol129921_4463.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D13329 Genomic DNA. Translation: BAA02590.1 .
    U14003 Genomic DNA. Translation: AAA97218.1 .
    U00096 Genomic DNA. Translation: AAC77278.1 .
    AP009048 Genomic DNA. Translation: BAE78315.1 .
    AJ225176 Genomic DNA. Translation: CAA12424.1 .
    X03411 Genomic DNA. Translation: CAA27147.1 . Different termination.
    X91735 Genomic DNA. Translation: CAA62860.1 .
    PIRi I57745.
    S56547.
    RefSeqi NP_418742.1. NC_000913.3.
    YP_492456.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4EAC X-ray 2.30 A/B/C/D 1-394 [» ]
    4EAY X-ray 2.35 A/B/C/D 1-394 [» ]
    ProteinModelPortali P24215.
    SMRi P24215. Positions 1-394.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-11108N.
    IntActi P24215. 2 interactions.
    STRINGi 511145.b4322.

    Proteomic databases

    PaxDbi P24215.
    PRIDEi P24215.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC77278 ; AAC77278 ; b4322 .
    BAE78315 ; BAE78315 ; BAE78315 .
    GeneIDi 12932009.
    947082.
    KEGGi ecj:Y75_p4208.
    eco:b4322.
    PATRICi 32124240. VBIEscCol129921_4463.

    Organism-specific databases

    EchoBASEi EB1059.
    EcoGenei EG11066. uxuA.

    Phylogenomic databases

    eggNOGi COG1312.
    HOGENOMi HOG000195381.
    KOi K01686.
    OMAi IPNGQVW.
    OrthoDBi EOG6ZSP6Z.
    PhylomeDBi P24215.

    Enzyme and pathway databases

    UniPathwayi UPA00246 .
    BioCyci EcoCyc:MANNONDEHYDRAT-MONOMER.
    ECOL316407:JW4285-MONOMER.
    MetaCyc:MANNONDEHYDRAT-MONOMER.

    Miscellaneous databases

    PROi P24215.

    Gene expression databases

    Genevestigatori P24215.

    Family and domain databases

    Gene3Di 3.20.20.150. 1 hit.
    HAMAPi MF_00106. UxuA.
    InterProi IPR004628. Man_deHydtase.
    IPR013022. Xyl_isomerase-like_TIM-brl.
    [Graphical view ]
    Pfami PF03786. UxuA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016049. Man_dehyd. 1 hit.
    SUPFAMi SSF51658. SSF51658. 2 hits.
    TIGRFAMsi TIGR00695. uxuA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Mizobuchi K.
      Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
      Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
      Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Colonization ability and pathogenic properties of a fim- mutant of an avian strain of Escherichia coli."
      Marc D., Arne P., Bree A., Dho-Moulin M.
      Res. Microbiol. 149:473-485(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-128.
      Strain: O2:K1:H+ / MT78.
    6. "The regulatory region of the uxuAB operon in Escherichia coli K12."
      Blanco C., Ritzenthaler P., Kolb A.
      Mol. Gen. Genet. 202:112-119(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
      Strain: K12.
    7. "The gntP gene of Escherichia coli involved in gluconate uptake."
      Klemm P., Tong S., Nielsen H., Conway T.
      J. Bacteriol. 178:61-67(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.
      Strain: K12.
    8. "The role of iron in the activation of mannonic and altronic acid hydratases, two Fe-requiring hydro-lyases."
      Dreyer J.L.
      Eur. J. Biochem. 166:623-630(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, PH DEPENDENCE, ENZYME REGULATION.
      Strain: K12.

    Entry informationi

    Entry nameiUXUA_ECOLI
    AccessioniPrimary (citable) accession number: P24215
    Secondary accession number(s): O87739, Q2M5Z1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3