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P24215 (UXUA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mannonate dehydratase

EC=4.2.1.8
Alternative name(s):
D-mannonate hydro-lyase
Gene names
Name:uxuA
Ordered Locus Names:b4322, JW4285
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length394 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the dehydration of D-mannonate. Ref.8

Catalytic activity

D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H2O. Ref.8

Cofactor

Fe2+ ion. Mn2+ can substitute for iron, but in higher concentrations. Cannot use Fe3+, Ni2+ or Mg2+. Ref.8

Enzyme regulation

Is inhibited by high concentrations of Fe2+ (> 2 mM), and by EDTA or other iron chelators in vitro. Ref.8

Pathway

Carbohydrate metabolism; pentose and glucuronate interconversion. HAMAP-Rule MF_00106

Induction

By fructuronate. Its expression is subjected to catabolite repression by glucose. Ref.8

Sequence similarities

Belongs to the mannonate dehydratase family.

Biophysicochemical properties

pH dependence:

Optimum pH is about 7.5. The activity at pH 6.5 and 8.6 is only about 20% of the maximal achievable activity at optimum pH. HAMAP-Rule MF_00106

Binary interactions

With

Entry

#Exp.

IntAct

Notes

nadEP188431EBI-1130634,EBI-548960

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 394394Mannonate dehydratase HAMAP-Rule MF_00106
PRO_0000170670

Natural variations

Natural variant521I → V in strain: MT78.

Secondary structure

............................................................ 394
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P24215 [UniParc].

Last modified February 1, 1995. Version 2.
Checksum: 0716A50BD6436679

FASTA39444,838
        10         20         30         40         50         60 
MEQTWRWYGP NDPVSLADVR QAGATGVVTA LHHIPNGEVW SVEEILKRKA IIEDAGLVWS 

        70         80         90        100        110        120 
VVESVPIHED IKTHTGNYEQ WIANYQQTLR NLAQCGIRTV CYNFMPVLDW TRTDLEYVLP 

       130        140        150        160        170        180 
DGSKALRFDQ IEFAAFEMHI LKRPGAEADY TEEEIAQAAE RFATMSDEDK ARLTRNIIAG 

       190        200        210        220        230        240 
LPGAEEGYTL DQFRKHLELY KDIDKAKLRE NFAVFLKAII PVAEEVGVRM AVHPDDPPRP 

       250        260        270        280        290        300 
ILGLPRIVST IEDMQWMVDT VNSMANGFTM CTGSYGVRAD NDLVDMIKQF GPRIYFTHLR 

       310        320        330        340        350        360 
STMREDNPKT FHEAAHLNGD VDMYEVVKAI VEEEHRRKAE GKEDLIPMRP DHGHQMLDDL 

       370        380        390 
KKKTNPGYSA IGRLKGLAEV RGVELAIQRA FFSR 

« Hide

References

« Hide 'large scale' references
[1]Mizobuchi K.
Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Colonization ability and pathogenic properties of a fim- mutant of an avian strain of Escherichia coli."
Marc D., Arne P., Bree A., Dho-Moulin M.
Res. Microbiol. 149:473-485(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-128.
Strain: O2:K1:H+ / MT78.
[6]"The regulatory region of the uxuAB operon in Escherichia coli K12."
Blanco C., Ritzenthaler P., Kolb A.
Mol. Gen. Genet. 202:112-119(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
Strain: K12.
[7]"The gntP gene of Escherichia coli involved in gluconate uptake."
Klemm P., Tong S., Nielsen H., Conway T.
J. Bacteriol. 178:61-67(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.
Strain: K12.
[8]"The role of iron in the activation of mannonic and altronic acid hydratases, two Fe-requiring hydro-lyases."
Dreyer J.L.
Eur. J. Biochem. 166:623-630(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, PH DEPENDENCE, ENZYME REGULATION.
Strain: K12.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13329 Genomic DNA. Translation: BAA02590.1.
U14003 Genomic DNA. Translation: AAA97218.1.
U00096 Genomic DNA. Translation: AAC77278.1.
AP009048 Genomic DNA. Translation: BAE78315.1.
AJ225176 Genomic DNA. Translation: CAA12424.1.
X03411 Genomic DNA. Translation: CAA27147.1. Different termination.
X91735 Genomic DNA. Translation: CAA62860.1.
PIRI57745.
S56547.
RefSeqNP_418742.1. NC_000913.3.
YP_492456.1. NC_007779.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4EACX-ray2.30A/B/C/D1-394[»]
4EAYX-ray2.35A/B/C/D1-394[»]
ProteinModelPortalP24215.
SMRP24215. Positions 1-394.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-11108N.
IntActP24215. 2 interactions.
STRING511145.b4322.

Proteomic databases

PaxDbP24215.
PRIDEP24215.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC77278; AAC77278; b4322.
BAE78315; BAE78315; BAE78315.
GeneID12932009.
947082.
KEGGecj:Y75_p4208.
eco:b4322.
PATRIC32124240. VBIEscCol129921_4463.

Organism-specific databases

EchoBASEEB1059.
EcoGeneEG11066. uxuA.

Phylogenomic databases

eggNOGCOG1312.
HOGENOMHOG000195381.
KOK01686.
OMAIPNGQVW.
OrthoDBEOG6ZSP6Z.
PhylomeDBP24215.

Enzyme and pathway databases

BioCycEcoCyc:MANNONDEHYDRAT-MONOMER.
ECOL316407:JW4285-MONOMER.
MetaCyc:MANNONDEHYDRAT-MONOMER.
UniPathwayUPA00246.

Gene expression databases

GenevestigatorP24215.

Family and domain databases

Gene3D3.20.20.150. 1 hit.
HAMAPMF_00106. UxuA.
InterProIPR004628. Man_deHydtase.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PfamPF03786. UxuA. 1 hit.
[Graphical view]
PIRSFPIRSF016049. Man_dehyd. 1 hit.
SUPFAMSSF51658. SSF51658. 2 hits.
TIGRFAMsTIGR00695. uxuA. 1 hit.
ProtoNetSearch...

Other

PROP24215.

Entry information

Entry nameUXUA_ECOLI
AccessionPrimary (citable) accession number: P24215
Secondary accession number(s): O87739, Q2M5Z1
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: February 1, 1995
Last modified: May 14, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene