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Protein

Phenylalanine-specific permease

Gene

pheP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Permease that is involved in the transport across the cytoplasmic membrane of phenylalanine.

GO - Molecular functioni

GO - Biological processi

  • L-alpha-amino acid transmembrane transport Source: GOC
  • L-amino acid transport Source: GOC
  • phenylalanine transport Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:PHEP-MONOMER.
ECOL316407:JW0565-MONOMER.
MetaCyc:PHEP-MONOMER.

Protein family/group databases

TCDBi2.A.3.1.1. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Phenylalanine-specific permease
Gene namesi
Name:pheP
Ordered Locus Names:b0576, JW0565
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10708. pheP.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2727CytoplasmicCuratedAdd
BLAST
Transmembranei28 – 4821Helical; Name=ICuratedAdd
BLAST
Topological domaini49 – 502PeriplasmicCurated
Transmembranei51 – 7121Helical; Name=IICuratedAdd
BLAST
Topological domaini72 – 10534CytoplasmicCuratedAdd
BLAST
Transmembranei106 – 12621Helical; Name=IIICuratedAdd
BLAST
Topological domaini127 – 1326PeriplasmicCurated
Transmembranei133 – 15321Helical; Name=IVCuratedAdd
BLAST
Topological domaini154 – 1607CytoplasmicCurated
Transmembranei161 – 18121Helical; Name=VCuratedAdd
BLAST
Topological domaini182 – 19615PeriplasmicCuratedAdd
BLAST
Transmembranei197 – 21721Helical; Name=VICuratedAdd
BLAST
Topological domaini218 – 25033CytoplasmicCuratedAdd
BLAST
Transmembranei251 – 27121Helical; Name=VIICuratedAdd
BLAST
Topological domaini272 – 28817PeriplasmicCuratedAdd
BLAST
Transmembranei289 – 30921Helical; Name=VIIICuratedAdd
BLAST
Topological domaini310 – 34132CytoplasmicCuratedAdd
BLAST
Transmembranei342 – 36221Helical; Name=IXCuratedAdd
BLAST
Topological domaini363 – 3675PeriplasmicCurated
Transmembranei368 – 38821Helical; Name=XCuratedAdd
BLAST
Topological domaini389 – 41123CytoplasmicCuratedAdd
BLAST
Transmembranei412 – 43221Helical; Name=XICuratedAdd
BLAST
Topological domaini433 – 4342PeriplasmicCurated
Transmembranei435 – 45521Helical; Name=XIICuratedAdd
BLAST
Topological domaini456 – 4583CytoplasmicCurated

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 458458Phenylalanine-specific permeasePRO_0000054206Add
BLAST

Proteomic databases

PaxDbiP24207.
PRIDEiP24207.

Interactioni

Protein-protein interaction databases

BioGridi4259879. 2 interactions.
DIPiDIP-10479N.
IntActiP24207. 1 interaction.
STRINGi511145.b0576.

Structurei

3D structure databases

ProteinModelPortaliP24207.
SMRiP24207. Positions 28-390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C85. Bacteria.
COG1113. LUCA.
HOGENOMiHOG000261849.
InParanoidiP24207.
KOiK11732.
OMAiIGFGLWM.
OrthoDBiEOG6HQSQM.
PhylomeDBiP24207.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24207-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNASTVSED TASNQEPTLH RGLHNRHIQL IALGGAIGTG LFLGIGPAIQ
60 70 80 90 100
MAGPAVLLGY GVAGIIAFLI MRQLGEMVVE EPVSGSFAHF AYKYWGPFAG
110 120 130 140 150
FLSGWNYWVM FVLVGMAELT AAGIYMQYWF PDVPTWIWAA AFFIIINAVN
160 170 180 190 200
LVNVRLYGET EFWFALIKVL AIIGMIGFGL WLLFSGHGGE KASIDNLWRY
210 220 230 240 250
GGFFATGWNG LILSLAVIMF SFGGLELIGI TAAEARDPEK SIPKAVNQVV
260 270 280 290 300
YRILLFYIGS LVVLLALYPW VEVKSNSSPF VMIFHNLDSN VVASALNFVI
310 320 330 340 350
LVASLSVYNS GVYSNSRMLF GLSVQGNAPK FLTRVSRRGV PINSLMLSGA
360 370 380 390 400
ITSLVVLINY LLPQKAFGLL MALVVATLLL NWIMICLAHL RFRAAMRRQG
410 420 430 440 450
RETQFKALLY PFGNYLCIAF LGMILLLMCT MDDMRLSAIL LPVWIVFLFM

AFKTLRRK
Length:458
Mass (Da):50,677
Last modified:March 1, 1992 - v1
Checksum:i69D865369249A1A8
GO

Sequence cautioni

The sequence AAB40774.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58000 Genomic DNA. Translation: AAA24334.1.
U82598 Genomic DNA. Translation: AAB40774.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73677.1.
AP009048 Genomic DNA. Translation: BAA35216.1.
PIRiA39431.
RefSeqiNP_415108.1. NC_000913.3.
WP_000786319.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73677; AAC73677; b0576.
BAA35216; BAA35216; BAA35216.
GeneIDi945199.
KEGGiecj:JW0565.
eco:b0576.
PATRICi32116320. VBIEscCol129921_0600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58000 Genomic DNA. Translation: AAA24334.1.
U82598 Genomic DNA. Translation: AAB40774.1. Different initiation.
U00096 Genomic DNA. Translation: AAC73677.1.
AP009048 Genomic DNA. Translation: BAA35216.1.
PIRiA39431.
RefSeqiNP_415108.1. NC_000913.3.
WP_000786319.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP24207.
SMRiP24207. Positions 28-390.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259879. 2 interactions.
DIPiDIP-10479N.
IntActiP24207. 1 interaction.
STRINGi511145.b0576.

Protein family/group databases

TCDBi2.A.3.1.1. the amino acid-polyamine-organocation (apc) family.

Proteomic databases

PaxDbiP24207.
PRIDEiP24207.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73677; AAC73677; b0576.
BAA35216; BAA35216; BAA35216.
GeneIDi945199.
KEGGiecj:JW0565.
eco:b0576.
PATRICi32116320. VBIEscCol129921_0600.

Organism-specific databases

EchoBASEiEB0702.
EcoGeneiEG10708. pheP.

Phylogenomic databases

eggNOGiENOG4105C85. Bacteria.
COG1113. LUCA.
HOGENOMiHOG000261849.
InParanoidiP24207.
KOiK11732.
OMAiIGFGLWM.
OrthoDBiEOG6HQSQM.
PhylomeDBiP24207.

Enzyme and pathway databases

BioCyciEcoCyc:PHEP-MONOMER.
ECOL316407:JW0565-MONOMER.
MetaCyc:PHEP-MONOMER.

Miscellaneous databases

PROiP24207.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR002293. AA/rel_permease1.
IPR004840. Amoino_acid_permease_CS.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF00324. AA_permease. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
PROSITEiPS00218. AMINO_ACID_PERMEASE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of the pheP gene, which encodes the phenylalanine-specific transport system of Escherichia coli."
    Pi J., Wookey P.J., Pittard A.J.
    J. Bacteriol. 173:3622-3629(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Topology of the phenylalanine-specific permease of Escherichia coli."
    Pi J., Pittard A.J.
    J. Bacteriol. 178:2650-2655(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiPHEP_ECOLI
AccessioniPrimary (citable) accession number: P24207
Secondary accession number(s): P77090
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: February 17, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.