Phenylalanine-specific permease - Escherichia coli (strain K12)

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P24207 (PHEP_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 112. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Phenylalanine-specific permease

Gene names

Name:	pheP
Ordered Locus Names:	b0576, JW0565

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	458 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Permease that is involved in the transport across the cytoplasmic membrane of phenylalanine.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family. [View classification]
Sequence caution	The sequence AAB40774.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	amino acid transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 458

458

Phenylalanine-specific permease

PRO_0000054206

Regions

Topological domain

1 – 27

Cytoplasmic Probable

Transmembrane

28 – 48

Helical; Name=I; Probable

Topological domain

49 – 50

Periplasmic Probable

Transmembrane

51 – 71

Helical; Name=II; Probable

Topological domain

72 – 105

Cytoplasmic Probable

Transmembrane

106 – 126

Helical; Name=III; Probable

Topological domain

127 – 132

Periplasmic Probable

Transmembrane

133 – 153

Helical; Name=IV; Probable

Topological domain

154 – 160

Cytoplasmic Probable

Transmembrane

161 – 181

Helical; Name=V; Probable

Topological domain

182 – 196

Periplasmic Probable

Transmembrane

197 – 217

Helical; Name=VI; Probable

Topological domain

218 – 250

Cytoplasmic Probable

Transmembrane

251 – 271

Helical; Name=VII; Probable

Topological domain

272 – 288

Periplasmic Probable

Transmembrane

289 – 309

Helical; Name=VIII; Probable

Topological domain

310 – 341

Cytoplasmic Probable

Transmembrane

342 – 362

Helical; Name=IX; Probable

Topological domain

363 – 367

Periplasmic Probable

Transmembrane

368 – 388

Helical; Name=X; Probable

Topological domain

389 – 411

Cytoplasmic Probable

Transmembrane

412 – 432

Helical; Name=XI; Probable

Topological domain

433 – 434

Periplasmic Probable

Transmembrane

435 – 455

Helical; Name=XII; Probable

Topological domain

456 – 458

Cytoplasmic Probable

Sequences

Sequence

Length

Mass (Da)

Tools

P24207 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 69D865369249A1A8
Show »

FASTA

458

50,677

        10         20         30         40         50         60 
MKNASTVSED TASNQEPTLH RGLHNRHIQL IALGGAIGTG LFLGIGPAIQ MAGPAVLLGY 

        70         80         90        100        110        120 
GVAGIIAFLI MRQLGEMVVE EPVSGSFAHF AYKYWGPFAG FLSGWNYWVM FVLVGMAELT 

       130        140        150        160        170        180 
AAGIYMQYWF PDVPTWIWAA AFFIIINAVN LVNVRLYGET EFWFALIKVL AIIGMIGFGL 

       190        200        210        220        230        240 
WLLFSGHGGE KASIDNLWRY GGFFATGWNG LILSLAVIMF SFGGLELIGI TAAEARDPEK 

       250        260        270        280        290        300 
SIPKAVNQVV YRILLFYIGS LVVLLALYPW VEVKSNSSPF VMIFHNLDSN VVASALNFVI 

       310        320        330        340        350        360 
LVASLSVYNS GVYSNSRMLF GLSVQGNAPK FLTRVSRRGV PINSLMLSGA ITSLVVLINY 

       370        380        390        400        410        420 
LLPQKAFGLL MALVVATLLL NWIMICLAHL RFRAAMRRQG RETQFKALLY PFGNYLCIAF 

       430        440        450 
LGMILLLMCT MDDMRLSAIL LPVWIVFLFM AFKTLRRK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the pheP gene, which encodes the phenylalanine-specific transport system of Escherichia coli." Pi J., Wookey P.J., Pittard A.J. J. Bacteriol. 173:3622-3629(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Sequence of minutes 4-25 of Escherichia coli." Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Topology of the phenylalanine-specific permease of Escherichia coli." Pi J., Pittard A.J. J. Bacteriol. 178:2650-2655(1996) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY.
[7]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M58000 Genomic DNA. Translation: AAA24334.1. U82598 Genomic DNA. Translation: AAB40774.1. Different initiation. U00096 Genomic DNA. Translation: AAC73677.1. AP009048 Genomic DNA. Translation: BAA35216.1.
PIR	A39431.
RefSeq	NP_415108.1. NC_000913.2. YP_488863.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P24207.
SMR	P24207. Positions 28-390.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-10479N.
IntAct	P24207. 1 interaction.
STRING	511145.b0576.
Protein family/group databases
TCDB	2.A.3.1.1. amino acid-polyamine-organocation (APC) family.
Proteomic databases
PRIDE	P24207.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC73677; AAC73677; b0576. BAA35216; BAA35216; BAA35216.
GeneID	12930885. 945199.
KEGG	ecj:Y75_p0563. eco:b0576.
PATRIC	32116320. VBIEscCol129921_0600.
Organism-specific databases
EchoBASE	EB0702.
EcoGene	EG10708. pheP.
Phylogenomic databases
eggNOG	COG1113.
HOGENOM	HOG000261849.
KO	K11732.
OMA	CIAFLGL.
ProtClustDB	PRK10249.
Enzyme and pathway databases
BioCyc	EcoCyc:PHEP-MONOMER. ECOL316407:JW0565-MONOMER.
Gene expression databases
Genevestigator	P24207.
Family and domain databases
InterPro	IPR004841. AA-permease_dom. IPR002293. AA/rel_permease1. IPR004840. Amoino_acid_permease_CS. [Graphical view]
PANTHER	PTHR11785. PTHR11785. 1 hit.
Pfam	PF00324. AA_permease. 1 hit. [Graphical view]
PIRSF	PIRSF006060. AA_transporter. 1 hit.
PROSITE	PS00218. AMINO_ACID_PERMEASE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHEP_ECOLI

Accession

Primary (citable) accession number: P24207
Secondary accession number(s): P77090

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	May 1, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents