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Protein

Lipid A biosynthesis myristoyltransferase

Gene

lpxM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of myristate from myristoyl-acyl carrier protein (ACP) to Kdo(2)-(lauroyl)-lipid IV(A) to form Kdo(2)-lipid A. Can probably also catalyze the transfer of myristate to Kdo(2)-(palmitoleoyl)-lipid IV(A) to form the cold-adapted Kdo(2)-lipid A. In vitro, can acylate Kdo(2)-lipid IV(A), but acylation of (KDO)2-(lauroyl)-lipid IV(A) is about 100 times faster. In vitro, can use lauroyl-ACP but displays a slight kinetic preference for myristoyl-ACP.1 Publication1 Publication

Catalytic activityi

A tetradecanoyl-[acyl-carrier protein] + (Kdo)(2)-(dodecanoyl)-lipid IV(A) = Kdo(2)-lipid A + a holo-[acyl-carrier protein].UniRule annotation1 Publication
A tetradecanoyl-[acyl-carrier protein] + Kdo(2)-(palmitoleoyl)-lipid IVA = Kdo(2)-lipid A, cold adapted + a holo-[acyl-carrier protein].1 Publication

Pathwayi: KDO(2)-lipid A biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A).UniRule annotation1 Publication1 Publication
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 3-deoxy-D-manno-octulosonic acid transferase (waaA)
  2. 3-deoxy-D-manno-octulosonic acid transferase (waaA)
  3. Lipid A biosynthesis lauroyltransferase (lpxL)
  4. Lipid A biosynthesis myristoyltransferase (lpxM)
This subpathway is part of the pathway KDO(2)-lipid A biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A), the pathway KDO(2)-lipid A biosynthesis and in Glycolipid biosynthesis.

Pathwayi: lipopolysaccharide biosynthesis

This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.UniRule annotation1 Publication1 Publication
View all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

GO - Biological processi

  • Kdo2-lipid A biosynthetic process Source: EcoCyc
  • lipopolysaccharide biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:MYRISTOYLACYLTRAN-MONOMER.
ECOL316407:JW1844-MONOMER.
MetaCyc:MYRISTOYLACYLTRAN-MONOMER.
BRENDAi2.3.1.B31. 2026.
UniPathwayiUPA00030.
UPA00360; UER00486.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid A biosynthesis myristoyltransferaseUniRule annotationCurated (EC:2.3.1.243UniRule annotation1 Publication1 Publication)
Alternative name(s):
Kdo(2)-lauroyl-lipid IV(A) myristoyltransferaseUniRule annotation
Gene namesi
Name:lpxM1 PublicationUniRule annotation
Synonyms:msbB1 Publication
Ordered Locus Names:b1855, JW1844
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10614. lpxM.

Subcellular locationi

  • Cell inner membrane UniRule annotation1 Publication; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei23 – 4321HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Makes pentaacylated lipid A rather than the usual hexaacylated lipid A.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323Lipid A biosynthesis myristoyltransferasePRO_0000201777Add
BLAST

Proteomic databases

PaxDbiP24205.
PRIDEiP24205.

Interactioni

Protein-protein interaction databases

BioGridi4260351. 224 interactions.
DIPiDIP-10262N.
IntActiP24205. 21 interactions.
MINTiMINT-1309336.
STRINGi511145.b1855.

Structurei

3D structure databases

ProteinModelPortaliP24205.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi139 – 1446HXXXXD motifUniRule annotationCurated

Sequence similaritiesi

Belongs to the LpxL/LpxM/LpxP family. LpxM subfamily.UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105NWR. Bacteria.
COG1560. LUCA.
HOGENOMiHOG000265962.
InParanoidiP24205.
KOiK02560.
OMAiQYVWILQ.
PhylomeDBiP24205.

Family and domain databases

CDDicd07984. LPLAT_LABLAT-like. 1 hit.
HAMAPiMF_01944. Lipid_A_LpxM. 1 hit.
InterProiIPR004960. LipA_acyltrans.
IPR011921. Lipid_A_MsbB.
[Graphical view]
PfamiPF03279. Lip_A_acyltrans. 1 hit.
[Graphical view]
PIRSFiPIRSF026649. MsbB. 1 hit.
TIGRFAMsiTIGR02208. lipid_A_msbB. 1 hit.

Sequencei

Sequence statusi: Complete.

P24205-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
METKKNNSEY IPEFDKSFRH PRYWGAWLGV AAMAGIALTP PKFRDPILAR
60 70 80 90 100
LGRFAGRLGK SSRRRALINL SLCFPERSEA EREAIVDEMF ATAPQAMAMM
110 120 130 140 150
AELAIRGPEK IQPRVDWQGL EIIEEMRRNN EKVIFLVPHG WAVDIPAMLM
160 170 180 190 200
ASQGQKMAAM FHNQGNPVFD YVWNTVRRRF GGRLHARNDG IKPFIQSVRQ
210 220 230 240 250
GYWGYYLPDQ DHGPEHSEFV DFFATYKATL PAIGRLMKVC RARVVPLFPI
260 270 280 290 300
YDGKTHRLTI QVRPPMDDLL EADDHTIARR MNEEVEIFVG PRPEQYTWIL
310 320
KLLKTRKPGE IQPYKRKDLY PIK
Length:323
Mass (Da):37,410
Last modified:March 1, 1992 - v1
Checksum:i94DAF38A757D20CD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77039 Genomic DNA. Translation: AAA24181.1.
M87660 Genomic DNA. Translation: AAA96706.1.
U00096 Genomic DNA. Translation: AAC74925.1.
AP009048 Genomic DNA. Translation: BAA15663.1.
PIRiA42608.
RefSeqiNP_416369.1. NC_000913.3.
WP_000448381.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74925; AAC74925; b1855.
BAA15663; BAA15663; BAA15663.
GeneIDi945143.
KEGGiecj:JW1844.
eco:b1855.
PATRICi32119033. VBIEscCol129921_1934.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77039 Genomic DNA. Translation: AAA24181.1.
M87660 Genomic DNA. Translation: AAA96706.1.
U00096 Genomic DNA. Translation: AAC74925.1.
AP009048 Genomic DNA. Translation: BAA15663.1.
PIRiA42608.
RefSeqiNP_416369.1. NC_000913.3.
WP_000448381.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP24205.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260351. 224 interactions.
DIPiDIP-10262N.
IntActiP24205. 21 interactions.
MINTiMINT-1309336.
STRINGi511145.b1855.

Proteomic databases

PaxDbiP24205.
PRIDEiP24205.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74925; AAC74925; b1855.
BAA15663; BAA15663; BAA15663.
GeneIDi945143.
KEGGiecj:JW1844.
eco:b1855.
PATRICi32119033. VBIEscCol129921_1934.

Organism-specific databases

EchoBASEiEB0609.
EcoGeneiEG10614. lpxM.

Phylogenomic databases

eggNOGiENOG4105NWR. Bacteria.
COG1560. LUCA.
HOGENOMiHOG000265962.
InParanoidiP24205.
KOiK02560.
OMAiQYVWILQ.
PhylomeDBiP24205.

Enzyme and pathway databases

UniPathwayiUPA00030.
UPA00360; UER00486.
BioCyciEcoCyc:MYRISTOYLACYLTRAN-MONOMER.
ECOL316407:JW1844-MONOMER.
MetaCyc:MYRISTOYLACYLTRAN-MONOMER.
BRENDAi2.3.1.B31. 2026.

Miscellaneous databases

PROiP24205.

Family and domain databases

CDDicd07984. LPLAT_LABLAT-like. 1 hit.
HAMAPiMF_01944. Lipid_A_LpxM. 1 hit.
InterProiIPR004960. LipA_acyltrans.
IPR011921. Lipid_A_MsbB.
[Graphical view]
PfamiPF03279. Lip_A_acyltrans. 1 hit.
[Graphical view]
PIRSFiPIRSF026649. MsbB. 1 hit.
TIGRFAMsiTIGR02208. lipid_A_msbB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLPXM_ECOLI
AccessioniPrimary (citable) accession number: P24205
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: September 7, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be the membrane-bound lytic murein transglycosylase (MLT).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.