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P24205 (MSBB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase
EC=2.3.1.-
Gene names
Name:msbB
Ordered Locus Names:b1855, JW1844
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transfers myristate or laurate, activated on ACP, to the lipid IVA moiety of (KDO)2-(lauroyl)-lipid IVA. Decanoyl, palmitoyl, palmitoleoyl, and (R)-3-hydroxymyristoyl-ACP are poor acyl donors. Functions optimally after laurate incorporation by HtrB has taken place. Acylates (KDO)2-(lauroyl)-lipid IVA about 100 times faster than (KDO)2-lipid IVA. Displays a preference for myristoyl-ACP over lauroyl-ACP. Ref.6

Pathway

Glycolipid biosynthesis; KDO(2)-lipid A biosynthesis; KDO(2)-lipid A from CMP-3-deoxy-D-manno-octulosonate and lipid IV(A): step 4/4.

Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.

Subcellular location

Cell inner membrane; Single-pass membrane protein Potential.

Sequence similarities

Belongs to the HtrB/MsbB family.

Caution

Was originally (Ref.2) thought to be the membrane-bound lytic murein transglycosylase (MLT).

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 323323Lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase
PRO_0000201777

Regions

Transmembrane23 – 4321Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
P24205 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 94DAF38A757D20CD

FASTA32337,410
        10         20         30         40         50         60 
METKKNNSEY IPEFDKSFRH PRYWGAWLGV AAMAGIALTP PKFRDPILAR LGRFAGRLGK 

        70         80         90        100        110        120 
SSRRRALINL SLCFPERSEA EREAIVDEMF ATAPQAMAMM AELAIRGPEK IQPRVDWQGL 

       130        140        150        160        170        180 
EIIEEMRRNN EKVIFLVPHG WAVDIPAMLM ASQGQKMAAM FHNQGNPVFD YVWNTVRRRF 

       190        200        210        220        230        240 
GGRLHARNDG IKPFIQSVRQ GYWGYYLPDQ DHGPEHSEFV DFFATYKATL PAIGRLMKVC 

       250        260        270        280        290        300 
RARVVPLFPI YDGKTHRLTI QVRPPMDDLL EADDHTIARR MNEEVEIFVG PRPEQYTWIL 

       310        320 
KLLKTRKPGE IQPYKRKDLY PIK

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of the Escherichia coli msbB gene, a multicopy suppressor of null mutations in the high-temperature requirement gene htrB."
Karow M.L., Georgopoulos C.
J. Bacteriol. 174:702-710(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"Murein-metabolizing enzymes from Escherichia coli: existence of a second lytic transglycosylase."
Engel H., Smink A.J., van Wijngaarden L., Keck W.
J. Bacteriol. 174:6394-6403(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map."
Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. expand/collapse author list , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Function of the Escherichia coli msbB gene, a multicopy suppressor of htrB knockouts, in the acylation of lipid A. Acylation by MsbB follows laurate incorporation by HtrB."
Clementz T., Zhou Z., Raetz C.R.H.
J. Biol. Chem. 272:10353-10360(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CHARACTERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M77039 Genomic DNA. Translation: AAA24181.1.
M87660 Genomic DNA. Translation: AAA96706.1.
U00096 Genomic DNA. Translation: AAC74925.1.
AP009048 Genomic DNA. Translation: BAA15663.1.
PIRA42608.
RefSeqNP_416369.1. NC_000913.2.
YP_490117.1. NC_007779.1.

3D structure databases

ProteinModelPortalP24205.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-10262N.
IntActP24205. 21 interactions.
MINTMINT-1309336.
STRING511145.b1855.

Proteomic databases

PaxDbP24205.
PRIDEP24205.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74925; AAC74925; b1855.
BAA15663; BAA15663; BAA15663.
GeneID12931357.
945143.
KEGGecj:Y75_p1831.
eco:b1855.
PATRIC32119033. VBIEscCol129921_1934.

Organism-specific databases

EchoBASEEB0609.
EcoGeneEG10614. msbB.

Phylogenomic databases

eggNOGCOG1560.
HOGENOMHOG000265962.
KOK02560.
OMAVWILQLL.
ProtClustDBPRK08943.

Enzyme and pathway databases

BioCycEcoCyc:MYRISTOYLACYLTRAN-MONOMER.
ECOL316407:JW1844-MONOMER.
MetaCyc:MYRISTOYLACYLTRAN-MONOMER.
UniPathwayUPA00030.
UPA00360; UER00486.

Gene expression databases

GenevestigatorP24205.

Family and domain databases

InterProIPR004960. LipA_acyltrans.
IPR011921. Lipid_A_MsbB.
[Graphical view]
PfamPF03279. Lip_A_acyltrans. 1 hit.
[Graphical view]
PIRSFPIRSF026649. MsbB. 1 hit.
TIGRFAMsTIGR02208. lipid_A_msbB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMSBB_ECOLI
AccessionPrimary (citable) accession number: P24205
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: May 1, 2013
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents