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Protein

P-loop guanosine triphosphatase YjiA

Gene

yjiA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds GTP and has low GTPase activity. May have a GTP-dependent regulatory function.2 Publications

Enzyme regulationi

GTPase activity is inhibited by metal binding. Activity is decreased in the presence of Co(II) or Ni(II), and is completely inhibited in the presence of Zn(II).1 Publication

Kineticsi

kcat is 0.006 sec(-1) for GTP hydrolysis in the absence of metal. kcat is 0.0005 sec(-1) for GTP hydrolysis in the presence of Co(II).1 Publication

      Sites

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Metal bindingi37Zinc 11 Publication1
      Metal bindingi42Zinc 11 Publication1
      Metal bindingi66Zinc 11 Publication1
      Metal bindingi74Zinc 2; shared with dimeric partner1 Publication1
      Metal bindingi114Zinc 2; shared with dimeric partner1 Publication1
      Binding sitei161GTPBy similarity1
      Metal bindingi167Zinc 31 Publication1
      Metal bindingi167Zinc 4; shared with dimeric partner1 Publication1
      Metal bindingi170Zinc 3; via pros nitrogen1 Publication1
      Metal bindingi170Zinc 4; via pros nitrogen; shared with dimeric partner1 Publication1
      Metal bindingi187Zinc 3; shared with dimeric partner1 Publication1
      Metal bindingi187Zinc 41 Publication1

      Regions

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Nucleotide bindingi11 – 19GTPBy similarity9

      GO - Molecular functioni

      • GTPase activity Source: EcoCyc
      • GTP binding Source: UniProtKB-KW
      • identical protein binding Source: EcoCyc
      • transition metal ion binding Source: EcoCyc

      GO - Biological processi

      • cellular response to DNA damage stimulus Source: EcoliWiki

      Keywordsi

      Molecular functionHydrolase
      LigandGTP-binding, Metal-binding, Nucleotide-binding, Zinc

      Enzyme and pathway databases

      BioCyciEcoCyc:EG10021-MONOMER
      MetaCyc:EG10021-MONOMER

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      P-loop guanosine triphosphatase YjiA1 Publication (EC:3.6.-.-2 Publications)
      Alternative name(s):
      GTP-binding protein YjiACurated
      Gene namesi
      Name:yjiA
      Ordered Locus Names:b4352, JW5790
      OrganismiEscherichia coli (strain K12)
      Taxonomic identifieri83333 [NCBI]
      Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
      Proteomesi
      • UP000000318 Componenti: Chromosome
      • UP000000625 Componenti: Chromosome

      Organism-specific databases

      EcoGeneiEG10021 yjiA

      Subcellular locationi

      GO - Cellular componenti

      • cytosol Source: EcoCyc

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      ChainiPRO_00001697851 – 318P-loop guanosine triphosphatase YjiASequence analysisAdd BLAST318

      Proteomic databases

      PaxDbiP24203
      PRIDEiP24203

      Interactioni

      Subunit structurei

      Monomer in the apo form. Metal binding induces oligomerization. Forms homodimers and higher oligomers.1 Publication

      GO - Molecular functioni

      • identical protein binding Source: EcoCyc

      Protein-protein interaction databases

      BioGridi4262771, 37 interactors
      DIPiDIP-12630N
      IntActiP24203, 2 interactors
      STRINGi316407.85677092

      Structurei

      Secondary structure

      1318
      Legend: HelixTurnBeta strandPDB Structure known for this area
      Show more details
      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Beta strandi4 – 16Combined sources13
      Helixi18 – 26Combined sources9
      Beta strandi33 – 36Combined sources4
      Beta strandi45 – 50Combined sources6
      Beta strandi56 – 60Combined sources5
      Beta strandi65 – 67Combined sources3
      Helixi73 – 85Combined sources13
      Beta strandi92 – 99Combined sources8
      Helixi104 – 113Combined sources10
      Helixi115 – 120Combined sources6
      Beta strandi121 – 131Combined sources11
      Turni132 – 134Combined sources3
      Helixi135 – 141Combined sources7
      Helixi143 – 150Combined sources8
      Beta strandi153 – 158Combined sources6
      Turni160 – 162Combined sources3
      Helixi167 – 176Combined sources10
      Beta strandi178 – 180Combined sources3
      Beta strandi182 – 184Combined sources3
      Beta strandi187 – 189Combined sources3
      Helixi192 – 195Combined sources4
      Helixi218 – 221Combined sources4
      Beta strandi223 – 233Combined sources11
      Helixi237 – 250Combined sources14
      Turni251 – 254Combined sources4
      Beta strandi255 – 262Combined sources8
      Beta strandi268 – 276Combined sources9
      Beta strandi279 – 287Combined sources9
      Beta strandi295 – 303Combined sources9
      Helixi306 – 314Combined sources9

      3D structure databases

      Select the link destinations:
      PDBei
      RCSB PDBi
      PDBji
      Links Updated
      PDB entryMethodResolution (Å)ChainPositionsPDBsum
      1NIJX-ray2.00A1-318[»]
      4IXMX-ray2.57A/B1-318[»]
      4IXNX-ray2.05A/B1-318[»]
      ProteinModelPortaliP24203
      SMRiP24203
      ModBaseiSearch...
      MobiDBiSearch...

      Miscellaneous databases

      EvolutionaryTraceiP24203

      Family & Domainsi

      Domains and Repeats

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Domaini224 – 315CobW C-terminalSequence analysisAdd BLAST92

      Sequence similaritiesi

      Phylogenomic databases

      eggNOGiENOG4105CKI Bacteria
      COG0523 LUCA
      HOGENOMiHOG000182211
      InParanoidiP24203
      OMAiTFFMDDE
      PhylomeDBiP24203

      Family and domain databases

      Gene3Di3.30.1220.10, 1 hit
      InterProiView protein in InterPro
      IPR011629 Cbl_biosynth_CobW-like_C
      IPR036627 CobW-likC_sf
      IPR003495 CobW/HypB/UreG_dom
      IPR027417 P-loop_NTPase
      PfamiView protein in Pfam
      PF02492 cobW, 1 hit
      PF07683 CobW_C, 1 hit
      SMARTiView protein in SMART
      SM00833 CobW_C, 1 hit
      SUPFAMiSSF52540 SSF52540, 1 hit

      Sequencei

      Sequence statusi: Complete.

      P24203-1 [UniParc]FASTAAdd to basket

      « Hide

              10         20         30         40         50
      MNPIAVTLLT GFLGAGKTTL LRHILNEQHG YKIAVIENEF GEVSVDDQLI
      60 70 80 90 100
      GDRATQIKTL TNGCICCSRS NELEDALLDL LDNLDKGNIQ FDRLVIECTG
      110 120 130 140 150
      MADPGPIIQT FFSHEVLCQR YLLDGVIALV DAVHADEQMN QFTIAQSQVG
      160 170 180 190 200
      YADRILLTKT DVAGEAEKLH ERLARINARA PVYTVTHGDI DLGLLFNTNG
      210 220 230 240 250
      FMLEENVVST KPRFHFIADK QNDISSIVVE LDYPVDISEV SRVMENLLLE
      260 270 280 290 300
      SADKLLRYKG MLWIDGEPNR LLFQGVQRLY SADWDRPWGD EKPHSTMVFI
      310
      GIQLPEEEIR AAFAGLRK
      Length:318
      Mass (Da):35,660
      Last modified:April 27, 2001 - v3
      Checksum:i6D68463182031C41
      GO

      Sequence cautioni

      The sequence AAA97249 differs from that shown. Reason: Frameshift at position 22.Curated

      Sequence databases

      Select the link destinations:
      EMBLi
      GenBanki
      DDBJi
      Links Updated
      U14003 Genomic DNA Translation: AAA97249.1 Frameshift.
      U00096 Genomic DNA Translation: AAC77308.2
      AP009048 Genomic DNA Translation: BAE78342.1
      X54198 Genomic DNA Translation: CAA38118.1
      PIRiS56578
      RefSeqiNP_418772.4, NC_000913.3
      WP_001312515.1, NZ_LN832404.1

      Genome annotation databases

      EnsemblBacteriaiAAC77308; AAC77308; b4352
      BAE78342; BAE78342; BAE78342
      GeneIDi948882
      KEGGiecj:JW5790
      eco:b4352
      PATRICifig|1411691.4.peg.2334

      Similar proteinsi

      Entry informationi

      Entry nameiYJIA_ECOLI
      AccessioniPrimary (citable) accession number: P24203
      Secondary accession number(s): Q2M5W4
      Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
      Last sequence update: April 27, 2001
      Last modified: April 25, 2018
      This is version 134 of the entry and version 3 of the sequence. See complete history.
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programProkaryotic Protein Annotation Program

      Miscellaneousi

      Keywords - Technical termi

      3D-structure, Complete proteome, Reference proteome
      UniProt is an ELIXIR core data resource
      Main funding by: National Institutes of Health