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Protein

5-methylcytosine-specific restriction enzyme A

Gene

mcrA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Restriction of 5-methyl and 5-hydroxymethylcytosines at the specific DNA sequence C(me)CGG.

GO - Molecular functioni

  • endonuclease activity Source: UniProtKB-KW
  • methyl-CpG binding Source: EcoliWiki

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Enzyme and pathway databases

BioCyciEcoCyc:EG10573-MONOMER.
ECOL316407:JW1145-MONOMER.
MetaCyc:EG10573-MONOMER.

Protein family/group databases

REBASEi2832. EcoKMcrA.

Names & Taxonomyi

Protein namesi
Recommended name:
5-methylcytosine-specific restriction enzyme A (EC:3.1.21.-)
Alternative name(s):
EcoKMcrA
Gene namesi
Name:mcrA
Synonyms:rglA
Ordered Locus Names:b1159, JW1145
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10573. mcrA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2772775-methylcytosine-specific restriction enzyme APRO_0000077380Add
BLAST

Proteomic databases

PaxDbiP24200.
PRIDEiP24200.

Interactioni

Protein-protein interaction databases

BioGridi4262863. 146 interactions.
IntActiP24200. 3 interactions.
STRINGi511145.b1159.

Structurei

3D structure databases

ProteinModelPortaliP24200.
SMRiP24200. Positions 224-259.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini207 – 25751HNHAdd
BLAST

Sequence similaritiesi

Contains 1 HNH domain.Curated

Phylogenomic databases

eggNOGiENOG4108R4U. Bacteria.
COG1403. LUCA.
KOiK07451.

Family and domain databases

InterProiIPR002711. HNH.
IPR003615. HNH_nuc.
[Graphical view]
PfamiPF01844. HNH. 1 hit.
[Graphical view]
SMARTiSM00507. HNHc. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24200-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHVFDNNGIE LKAECSIGEE DGVYGLILES WGPGDRNKDY NIALDYIIER
60 70 80 90 100
LVDSGVSQVV VYLASSSVRK HMHSLDERKI HPGEYFTLIG NSPRDIRLKM
110 120 130 140 150
CGYQAYFSRT GRKEIPSGNR TKRILINVPG IYSDSFWASI IRGELSELSQ
160 170 180 190 200
PTDDESLLNM RVSKLIKKTL SQPEGSRKPV EVERLQKVYV RDPMVKAWIL
210 220 230 240 250
QQSKGICENC GKNAPFYLND GNPYLEVHHV IPLSSGGADT TDNCVALCPN
260 270
CHRELHYSKN AKELIEMLYV NINRLQK
Length:277
Mass (Da):31,390
Last modified:November 1, 1997 - v2
Checksum:iE5F2627DFFDEC402
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti214 – 2141A → R (PubMed:1938927).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76667 Genomic DNA. Translation: AAA68481.1.
Z19104 Genomic DNA. Translation: CAA79520.1.
U00096 Genomic DNA. Translation: AAC74243.1.
AP009048 Genomic DNA. Translation: BAA35995.1.
PIRiA41424.
RefSeqiNP_415677.1. NC_000913.3.
WP_000557907.1. NZ_CP010440.1.

Genome annotation databases

EnsemblBacteriaiAAC74243; AAC74243; b1159.
BAA35995; BAA35995; BAA35995.
GeneIDi945727.
KEGGiecj:JW1145.
eco:b1159.
PATRICi32117555. VBIEscCol129921_1200.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76667 Genomic DNA. Translation: AAA68481.1.
Z19104 Genomic DNA. Translation: CAA79520.1.
U00096 Genomic DNA. Translation: AAC74243.1.
AP009048 Genomic DNA. Translation: BAA35995.1.
PIRiA41424.
RefSeqiNP_415677.1. NC_000913.3.
WP_000557907.1. NZ_CP010440.1.

3D structure databases

ProteinModelPortaliP24200.
SMRiP24200. Positions 224-259.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262863. 146 interactions.
IntActiP24200. 3 interactions.
STRINGi511145.b1159.

Protein family/group databases

REBASEi2832. EcoKMcrA.

Proteomic databases

PaxDbiP24200.
PRIDEiP24200.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74243; AAC74243; b1159.
BAA35995; BAA35995; BAA35995.
GeneIDi945727.
KEGGiecj:JW1145.
eco:b1159.
PATRICi32117555. VBIEscCol129921_1200.

Organism-specific databases

EchoBASEiEB0568.
EcoGeneiEG10573. mcrA.

Phylogenomic databases

eggNOGiENOG4108R4U. Bacteria.
COG1403. LUCA.
KOiK07451.

Enzyme and pathway databases

BioCyciEcoCyc:EG10573-MONOMER.
ECOL316407:JW1145-MONOMER.
MetaCyc:EG10573-MONOMER.

Miscellaneous databases

PROiP24200.

Family and domain databases

InterProiIPR002711. HNH.
IPR003615. HNH_nuc.
[Graphical view]
PfamiPF01844. HNH. 1 hit.
[Graphical view]
SMARTiSM00507. HNHc. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCRA_ECOLI
AccessioniPrimary (citable) accession number: P24200
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.