Reviewed, UniProtKB/Swiss-Prot P24200 (MCRA_ECOLI)

Names and origin

Protein names

Recommended name:
    5-methylcytosine-specific restriction enzyme A
    EC=3.1.21.-
Alternative name(s):
    EcoKMcrA

Gene names

Name:	mcrA
Synonyms:	rglA
Ordered Locus Names:	b1159, JW1145

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Predicted.

General annotation (Comments)

Function

Restriction of 5-methyl and 5-hydroxymethylcytosines at the specific DNA sequence C(me)CGG.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	5-methylcytosine-specific restriction enzyme A		PRO_0000077380
Experimental info
	Sequence conflict	214	1	A → R Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P24200-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: E5F2627DFFDEC402	FASTA	277	31,390
	10 20 30 40 50 60 MHVFDNNGIE LKAECSIGEE DGVYGLILES WGPGDRNKDY NIALDYIIER LVDSGVSQVV 70 80 90 100 110 120 VYLASSSVRK HMHSLDERKI HPGEYFTLIG NSPRDIRLKM CGYQAYFSRT GRKEIPSGNR 130 140 150 160 170 180 TKRILINVPG IYSDSFWASI IRGELSELSQ PTDDESLLNM RVSKLIKKTL SQPEGSRKPV 190 200 210 220 230 240 EVERLQKVYV RDPMVKAWIL QQSKGICENC GKNAPFYLND GNPYLEVHHV IPLSSGGADT 250 260 270 TDNCVALCPN CHRELHYSKN AKELIEMLYV NINRLQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and structural characterization of the mcrA locus of Escherichia coli." Hiom K.J., Sedgwick S.G. J. Bacteriol. 173:7368-7373(1991) [PubMed: 1938927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular cloning and sequencing of mcrA locus and identification of McrA protein in Escherichia coli." Ramalingam R., Prasad R., Shivapriya R., Dharmalingam K. J. Biosci. 17:217-232(1992) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M76667 Genomic DNA. Translation: AAA68481.1. Z19104 Genomic DNA. Translation: CAA79520.1. U00096 Genomic DNA. Translation: AAC74243.1. AP009048 Genomic DNA. Translation: BAA35995.1.
PIR	A41424.
RefSeq	AP_001784.1. NP_415677.1.
3D structure databases
SMR	P24200. Positions 196-254.
ModBase	Search...
Protein-protein interaction databases
STRING	P24200.
Protein family/group databases
REBASE	2832. EcoKMcrA.
Genome annotation databases
GeneID	945727.
GenomeReviews	Gene locus JW1145 in contig AP009048_GR. Gene locus b1159 in contig U00096_GR.
KEGG	ecj:JW1145. eco:b1159.
Organism-specific databases
EchoBASE	EB0568.
EcoGene	EG10573. mcrA.
CMR	Search...
Phylogenomic databases
eggNOG	COG1403.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10573-MONOMER. ECOL168927:B1159-MONOMER. MetaCyc:EG10573-MONOMER.
Gene expression databases
Genevestigator	P24200.
Family and domain databases
InterPro	IPR002711. HNH. IPR003615. HNH_nuc. [Graphical view]
Pfam	PF01844. HNH. 1 hit. [Graphical view]
SMART	SM00507. HNHc. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCRA_ECOLI

Accession

Primary (citable) accession number: P24200

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	November 1, 1997
Last modified:	January 19, 2010
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)