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Protein

Hydrogenase isoenzymes formation protein HypE

Gene

hypE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

GO - Biological processi

  • peptidyl-S-carbamoyl-L-cysteine dehydration Source: EcoCyc
  • protein maturation Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:EG10487-MONOMER.
ECOL316407:JW2700-MONOMER.
MetaCyc:EG10487-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase isoenzymes formation protein HypE
Gene namesi
Name:hypE
Ordered Locus Names:b2730, JW2700
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10487. hypE.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 336336Hydrogenase isoenzymes formation protein HypEPRO_0000201459Add
BLAST

Proteomic databases

PaxDbiP24193.

Interactioni

Protein-protein interaction databases

BioGridi4261424. 5 interactions.
DIPiDIP-9999N.
IntActiP24193. 10 interactions.
MINTiMINT-1239514.
STRINGi511145.b2730.

Structurei

Secondary structure

1
336
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi15 – 2713Combined sources
Turni30 – 323Combined sources
Beta strandi37 – 426Combined sources
Helixi43 – 497Combined sources
Beta strandi51 – 6010Combined sources
Beta strandi63 – 675Combined sources
Helixi72 – 8514Combined sources
Turni86 – 883Combined sources
Beta strandi93 – 1019Combined sources
Helixi106 – 12217Combined sources
Beta strandi126 – 13510Combined sources
Beta strandi139 – 15315Combined sources
Helixi161 – 1633Combined sources
Beta strandi169 – 1746Combined sources
Helixi178 – 18811Combined sources
Helixi204 – 2074Combined sources
Turni208 – 2125Combined sources
Beta strandi216 – 2216Combined sources
Helixi226 – 23712Combined sources
Beta strandi240 – 2456Combined sources
Turni246 – 2483Combined sources
Helixi253 – 26210Combined sources
Turni266 – 2683Combined sources
Beta strandi275 – 2795Combined sources
Helixi281 – 2833Combined sources
Helixi284 – 2929Combined sources
Helixi295 – 2973Combined sources
Beta strandi301 – 3099Combined sources
Beta strandi311 – 3155Combined sources
Helixi317 – 3193Combined sources
Beta strandi321 – 3233Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I6RX-ray2.51A/B/C/D15-336[»]
ProteinModelPortaliP24193.
SMRiP24193. Positions 14-336.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24193.

Family & Domainsi

Sequence similaritiesi

Belongs to the HypE family.Curated

Phylogenomic databases

eggNOGiENOG4105C45. Bacteria.
COG0309. LUCA.
HOGENOMiHOG000278777.
InParanoidiP24193.
KOiK04655.
OrthoDBiEOG6BW4W5.
PhylomeDBiP24193.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR011854. HypE.
IPR016188. PurM-like_N.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005644. Hdrgns_mtr_HypE. 1 hit.
SUPFAMiSSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR02124. hypE. 1 hit.

Sequencei

Sequence statusi: Complete.

P24193-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNIQLAHGS GGQAMQQLIN SLFMEAFANP WLAEQEDQAR LDLAQLVAEG
60 70 80 90 100
DRLAFSTDSY VIDPLFFPGG NIGKLAICGT ANDVAVSGAI PRYLSCGFIL
110 120 130 140 150
EEGLPMETLK AVVTSMAETA RAAGIAIVTG DTKVVQRGAV DKLFINTAGM
160 170 180 190 200
GAIPANIHWG AQTLTAGDVL LVSGTLGDHG ATILNLREQL GLDGELVSDC
210 220 230 240 250
AVLTPLIQTL RDIPGVKALR DATRGGVNAV VHEFAAACGC GIELSEAALP
260 270 280 290 300
VKPAVRGVCE LLGLDALNFA NEGKLVIAVE RNAAEQVLAA LHSHPLGKDA
310 320 330
ALIGEVVERK GVRLAGLYGV KRTLDLPHAE PLPRIC
Length:336
Mass (Da):35,092
Last modified:May 5, 2009 - v2
Checksum:i0811EE415F11AF6C
GO

Sequence cautioni

The sequence AAA69240.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAE76807.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA38416.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54543 Genomic DNA. Translation: CAA38416.1. Different initiation.
U29579 Genomic DNA. Translation: AAA69240.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75772.2.
AP009048 Genomic DNA. Translation: BAE76807.1. Different initiation.
PIRiS15201.
RefSeqiNP_417210.2. NC_000913.3.
WP_001059922.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75772; AAC75772; b2730.
BAE76807; BAE76807; BAE76807.
GeneIDi947182.
KEGGiecj:JW2700.
eco:b2730.
PATRICi32120860. VBIEscCol129921_2822.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54543 Genomic DNA. Translation: CAA38416.1. Different initiation.
U29579 Genomic DNA. Translation: AAA69240.1. Different initiation.
U00096 Genomic DNA. Translation: AAC75772.2.
AP009048 Genomic DNA. Translation: BAE76807.1. Different initiation.
PIRiS15201.
RefSeqiNP_417210.2. NC_000913.3.
WP_001059922.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2I6RX-ray2.51A/B/C/D15-336[»]
ProteinModelPortaliP24193.
SMRiP24193. Positions 14-336.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261424. 5 interactions.
DIPiDIP-9999N.
IntActiP24193. 10 interactions.
MINTiMINT-1239514.
STRINGi511145.b2730.

Proteomic databases

PaxDbiP24193.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75772; AAC75772; b2730.
BAE76807; BAE76807; BAE76807.
GeneIDi947182.
KEGGiecj:JW2700.
eco:b2730.
PATRICi32120860. VBIEscCol129921_2822.

Organism-specific databases

EchoBASEiEB0482.
EcoGeneiEG10487. hypE.

Phylogenomic databases

eggNOGiENOG4105C45. Bacteria.
COG0309. LUCA.
HOGENOMiHOG000278777.
InParanoidiP24193.
KOiK04655.
OrthoDBiEOG6BW4W5.
PhylomeDBiP24193.

Enzyme and pathway databases

BioCyciEcoCyc:EG10487-MONOMER.
ECOL316407:JW2700-MONOMER.
MetaCyc:EG10487-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP24193.
PROiP24193.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.90.650.10. 1 hit.
InterProiIPR010918. AIR_synth_C_dom.
IPR011854. HypE.
IPR016188. PurM-like_N.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005644. Hdrgns_mtr_HypE. 1 hit.
SUPFAMiSSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR02124. hypE. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of an operon (hyp) necessary for the activity of the three hydrogenase isoenzymes in Escherichia coli."
    Lutz S., Jacobi A., Schlensog V., Boehm R., Sawers G., Boeck A.
    Mol. Microbiol. 5:123-135(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.

Entry informationi

Entry nameiHYPE_ECOLI
AccessioniPrimary (citable) accession number: P24193
Secondary accession number(s): Q2MA99, Q46886
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: May 5, 2009
Last modified: January 20, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.