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Protein

Hydrogenase maturation factor HypD

Gene

hypD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the maturation of [NiFe] hydrogenases. Involved in the biosynthesis of the Fe(CN)2CO cofactor (PubMed:15504408, PubMed:23022438). HypD may act as a scaffold on which the Fe(CN)2CO cofactor is formed (PubMed:23597401). In complex with HypC, accepts the cyanide ligand generated by HypF and HypE, and also coordinates the carbon monoxide ligand (PubMed:15504408, PubMed:23022438). Required for the formation of all three hydrogenase isoenzymes (Probable).Curated3 Publications

Cofactori

[4Fe-4S] cluster2 Publications

Pathwayi: [NiFe] hydrogenase maturation

This protein is involved in the pathway [NiFe] hydrogenase maturation, which is part of Protein modification.Curated
View all proteins of this organism that are known to be involved in the pathway [NiFe] hydrogenase maturation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi41Iron1 Publication1
Metal bindingi69Iron2 Publications1
Metal bindingi72Iron2 Publications1

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • carbon monoxide binding Source: EcoCyc
  • iron ion binding Source: EcoCyc

GO - Biological processi

  • cellular protein modification process Source: EcoCyc
  • protein maturation Source: EcoCyc

Keywordsi

Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10486-MONOMER
UniPathwayiUPA00335

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase maturation factor HypDCurated
Alternative name(s):
Hydrogenase isoenzymes formation protein HypDCurated
Gene namesi
Name:hypD1 Publication
Ordered Locus Names:b2729, JW2699
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10486 hypD

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi41C → A: Does not affect interaction with HypC. Retains the 4Fe-4S cluster, but has less iron than the native complex. Can no longer coordinate CN(-1) and CO. 2 Publications1
Mutagenesisi69C → A: Can no longer coordinate CN(-1) and CO. Reduces iron content. 1 Publication1
Mutagenesisi72C → A: Can no longer coordinate CN(-1) and CO. Reduces iron content. 1 Publication1
Mutagenesisi357E → A or D: Does not affect CN(-1) and CO coordination. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002014511 – 373Hydrogenase maturation factor HypDAdd BLAST373

Proteomic databases

PaxDbiP24192
PRIDEiP24192

2D gel databases

SWISS-2DPAGEiP24192

Interactioni

Subunit structurei

Monomer (PubMed:15504408). Interacts with HypC (PubMed:12441107, PubMed:15504408, PubMed:23022438). Forms a complex with HypC, or HybG, and HypE (PubMed:15504408, PubMed:16412426).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
hybGP0AAM77EBI-552711,EBI-562426

Protein-protein interaction databases

BioGridi4261425, 34 interactors
DIPiDIP-9998N
IntActiP24192, 12 interactors
STRINGi316385.ECDH10B_2897

Structurei

3D structure databases

ProteinModelPortaliP24192
SMRiP24192
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

In addition to the iron-sulfur cluster, contains two additional oxygen-labile iron ions.1 Publication

Sequence similaritiesi

Belongs to the HypD family.Curated

Phylogenomic databases

eggNOGiENOG4105CQS Bacteria
COG0409 LUCA
HOGENOMiHOG000278766
InParanoidiP24192
KOiK04654
OMAiHVCGSHE
PhylomeDBiP24192

Family and domain databases

InterProiView protein in InterPro
IPR002780 Hyd_form_HypD
PANTHERiPTHR30149 PTHR30149, 1 hit
PfamiView protein in Pfam
PF01924 HypD, 1 hit
PIRSFiPIRSF005622 Hydrgn_mat_hypD, 1 hit
TIGRFAMsiTIGR00075 hypD, 1 hit

Sequencei

Sequence statusi: Complete.

P24192-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFVDEYRAP EQVMQLIEHL RERASHLSYT AERPLRIMEV CGGHTHAIFK
60 70 80 90 100
FGLDQLLPEN VEFIHGPGCP VCVLPMGRID TCVEIASHPE VIFCTFGDAM
110 120 130 140 150
RVPGKQGSLL QAKARGADVR IVYSPMDALK LAQENPTRKV VFFGLGFETT
160 170 180 190 200
MPTTAITLQQ AKARDVQNFY FFCQHITLIP TLRSLLEQPD NGIDAFLAPG
210 220 230 240 250
HVSMVIGTDA YNFIASDFHR PLVVAGFEPL DLLQGVVMLV QQKIAAHSKV
260 270 280 290 300
ENQYRRVVPD AGNLLAQQAI ADVFCVNGDS EWRGLGVIES SGVHLTPDYQ
310 320 330 340 350
RFDAEAHFRP APQQVCDDPR ARCGEVLTGK CKPHQCPLFG NTCNPQTAFG
360 370
ALMVSSEGAC AAWYQYRQQE SEA
Length:373
Mass (Da):41,363
Last modified:March 1, 1992 - v1
Checksum:i0E4186081FAA4C54
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X54543 Genomic DNA Translation: CAA38415.1
U29579 Genomic DNA Translation: AAA69239.1
U00096 Genomic DNA Translation: AAC75771.1
AP009048 Genomic DNA Translation: BAE76806.1
PIRiS15200
RefSeqiNP_417209.1, NC_000913.3
WP_001212985.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75771; AAC75771; b2729
BAE76806; BAE76806; BAE76806
GeneIDi947189
KEGGiecj:JW2699
eco:b2729
PATRICifig|1411691.4.peg.4012

Similar proteinsi

Entry informationi

Entry nameiHYPD_ECOLI
AccessioniPrimary (citable) accession number: P24192
Secondary accession number(s): Q2MAA0, Q46885
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: March 28, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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