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Protein

Bifunctional protein FolD

Gene

folD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate. This enzyme is specific for NADP.UniRule annotation1 Publication

Catalytic activityi

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH.UniRule annotation
5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate.UniRule annotation

Pathwayi: tetrahydrofolate interconversion

This protein is involved in the pathway tetrahydrofolate interconversion, which is part of One-carbon metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tetrahydrofolate interconversion and in One-carbon metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei232NADP; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi166 – 168NADPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis, Methionine biosynthesis, One-carbon metabolism, Purine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciEcoCyc:FOLD-MONOMER.
ECOL316407:JW0518-MONOMER.
MetaCyc:FOLD-MONOMER.
SABIO-RKP24186.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein FolDUniRule annotation
Including the following 2 domains:
Methylenetetrahydrofolate dehydrogenaseUniRule annotation (EC:1.5.1.5UniRule annotation)
Methenyltetrahydrofolate cyclohydrolaseUniRule annotation (EC:3.5.4.9UniRule annotation)
Gene namesi
Name:folDUniRule annotation
Synonyms:ads
Ordered Locus Names:b0529, JW0518
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10328. folD.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001993062 – 288Bifunctional protein FolDAdd BLAST287

Proteomic databases

PaxDbiP24186.
PRIDEiP24186.

Interactioni

Subunit structurei

Homodimer.UniRule annotation2 Publications

Protein-protein interaction databases

BioGridi4262819. 252 interactors.
DIPiDIP-9675N.
IntActiP24186. 4 interactors.
STRINGi511145.b0529.

Structurei

Secondary structure

1288
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 28Combined sources21
Beta strandi35 – 42Combined sources8
Helixi45 – 61Combined sources17
Beta strandi68 – 70Combined sources3
Helixi76 – 87Combined sources12
Beta strandi94 – 97Combined sources4
Helixi107 – 111Combined sources5
Turni116 – 118Combined sources3
Helixi125 – 132Combined sources8
Helixi141 – 152Combined sources12
Beta strandi161 – 165Combined sources5
Turni169 – 171Combined sources3
Helixi172 – 180Combined sources9
Turni181 – 183Combined sources3
Beta strandi185 – 189Combined sources5
Helixi196 – 202Combined sources7
Beta strandi204 – 208Combined sources5
Turni218 – 220Combined sources3
Beta strandi226 – 229Combined sources4
Helixi246 – 252Combined sources7
Beta strandi254 – 256Combined sources3
Beta strandi259 – 263Combined sources5
Helixi264 – 281Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B0AX-ray2.56A1-288[»]
ProteinModelPortaliP24186.
SMRiP24186.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24186.

Family & Domainsi

Sequence similaritiesi

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CN0. Bacteria.
COG0190. LUCA.
HOGENOMiHOG000218242.
InParanoidiP24186.
KOiK01491.
OMAiGQPMALM.
PhylomeDBiP24186.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24186-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAKIIDGKT IAQQVRSEVA QKVQARIAAG LRAPGLAVVL VGSNPASQIY
60 70 80 90 100
VASKRKACEE VGFVSRSYDL PETTSEAELL ELIDTLNADN TIDGILVQLP
110 120 130 140 150
LPAGIDNVKV LERIHPDKDV DGFHPYNVGR LCQRAPRLRP CTPRGIVTLL
160 170 180 190 200
ERYNIDTFGL NAVVIGASNI VGRPMSMELL LAGCTTTVTH RFTKNLRHHV
210 220 230 240 250
ENADLLIVAV GKPGFIPGDW IKEGAIVIDV GINRLENGKV VGDVVFEDAA
260 270 280
KRASYITPVP GGVGPMTVAT LIENTLQACV EYHDPQDE
Length:288
Mass (Da):31,044
Last modified:January 23, 2007 - v4
Checksum:i028039D7C611085D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47S → L in AAB40282 (Ref. 3) Curated1
Sequence conflicti200V → L in AAA23803 (PubMed:1748668).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74789 Genomic DNA. Translation: AAA23803.1.
D10588 Genomic DNA. Translation: BAA01445.1.
U82664 Genomic DNA. Translation: AAB40282.1.
U00096 Genomic DNA. Translation: AAC73631.1.
AP009048 Genomic DNA. Translation: BAE76306.1.
PIRiH64784. JS0662.
RefSeqiNP_415062.1. NC_000913.3.
WP_000729160.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73631; AAC73631; b0529.
BAE76306; BAE76306; BAE76306.
GeneIDi945221.
KEGGiecj:JW0518.
eco:b0529.
PATRICi32116218. VBIEscCol129921_0550.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74789 Genomic DNA. Translation: AAA23803.1.
D10588 Genomic DNA. Translation: BAA01445.1.
U82664 Genomic DNA. Translation: AAB40282.1.
U00096 Genomic DNA. Translation: AAC73631.1.
AP009048 Genomic DNA. Translation: BAE76306.1.
PIRiH64784. JS0662.
RefSeqiNP_415062.1. NC_000913.3.
WP_000729160.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B0AX-ray2.56A1-288[»]
ProteinModelPortaliP24186.
SMRiP24186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262819. 252 interactors.
DIPiDIP-9675N.
IntActiP24186. 4 interactors.
STRINGi511145.b0529.

Proteomic databases

PaxDbiP24186.
PRIDEiP24186.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73631; AAC73631; b0529.
BAE76306; BAE76306; BAE76306.
GeneIDi945221.
KEGGiecj:JW0518.
eco:b0529.
PATRICi32116218. VBIEscCol129921_0550.

Organism-specific databases

EchoBASEiEB0324.
EcoGeneiEG10328. folD.

Phylogenomic databases

eggNOGiENOG4105CN0. Bacteria.
COG0190. LUCA.
HOGENOMiHOG000218242.
InParanoidiP24186.
KOiK01491.
OMAiGQPMALM.
PhylomeDBiP24186.

Enzyme and pathway databases

UniPathwayiUPA00193.
BioCyciEcoCyc:FOLD-MONOMER.
ECOL316407:JW0518-MONOMER.
MetaCyc:FOLD-MONOMER.
SABIO-RKP24186.

Miscellaneous databases

EvolutionaryTraceiP24186.
PROiP24186.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_01576. THF_DHG_CYH. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamiPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSiPR00085. THFDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFOLD_ECOLI
AccessioniPrimary (citable) accession number: P24186
Secondary accession number(s): P77132, Q2MBQ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 136 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.