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Protein

Multidrug export protein AcrF

Gene

acrF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the tripartite efflux system AcrEF-TolC. Involved in the efflux of indole and organic solvents.2 Publications

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:ACRF-MONOMER.
ECOL316407:JW3234-MONOMER.

Protein family/group databases

TCDBi2.A.6.2.1. the resistance-nodulation-cell division (rnd) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug export protein AcrF
Alternative name(s):
Acriflavine resistance protein F
Protein EnvD
Gene namesi
Name:acrF
Synonyms:envD
Ordered Locus Names:b3266, JW3234
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10267. acrF.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicBy similarity
Transmembranei10 – 2819Helical; Name=1By similarityAdd
BLAST
Topological domaini29 – 339311PeriplasmicBy similarityAdd
BLAST
Transmembranei340 – 35920Helical; Name=2By similarityAdd
BLAST
Topological domaini360 – 3656CytoplasmicBy similarity
Transmembranei366 – 38520Helical; Name=3By similarityAdd
BLAST
Topological domaini386 – 3916PeriplasmicBy similarity
Transmembranei392 – 41322Helical; Name=4By similarityAdd
BLAST
Topological domaini414 – 44128CytoplasmicBy similarityAdd
BLAST
Transmembranei442 – 46019Helical; Name=5By similarityAdd
BLAST
Topological domaini461 – 47313PeriplasmicBy similarityAdd
BLAST
Transmembranei474 – 49623Helical; Name=6By similarityAdd
BLAST
Topological domaini497 – 53741CytoplasmicBy similarityAdd
BLAST
Transmembranei538 – 55619Helical; Name=7By similarityAdd
BLAST
Topological domaini557 – 871315PeriplasmicBy similarityAdd
BLAST
Transmembranei872 – 89120Helical; Name=8By similarityAdd
BLAST
Topological domaini892 – 8976CytoplasmicBy similarity
Transmembranei898 – 91720Helical; Name=9By similarityAdd
BLAST
Topological domaini918 – 9236PeriplasmicBy similarity
Transmembranei924 – 94522Helical; Name=10By similarityAdd
BLAST
Topological domaini946 – 97328CytoplasmicBy similarityAdd
BLAST
Transmembranei974 – 99219Helical; Name=11By similarityAdd
BLAST
Topological domaini993 – 100513PeriplasmicBy similarityAdd
BLAST
Transmembranei1006 – 102823Helical; Name=12By similarityAdd
BLAST
Topological domaini1029 – 10346CytoplasmicBy similarity

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10341034Multidrug export protein AcrFPRO_0000161813Add
BLAST

Proteomic databases

PaxDbiP24181.
PRIDEiP24181.

Expressioni

Inductioni

Induced by LeuO, part of the acrEF operon.1 Publication

Interactioni

Subunit structurei

Part of the tripartite efflux system AcrEF-TolC, which is composed of an inner membrane transporter, AcrF, a periplasmic membrane fusion protein, AcrE, and an outer membrane component, TolC. The complex forms a large protein conduit and can translocate molecules across both the inner and outer membranes (Probable).1 Publication

Protein-protein interaction databases

BioGridi4262457. 308 interactions.
DIPiDIP-9052N.
IntActiP24181. 1 interaction.
MINTiMINT-1267966.
STRINGi511145.b3266.

Structurei

3D structure databases

ProteinModelPortaliP24181.
SMRiP24181. Positions 1-1018.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZS. Bacteria.
COG0841. LUCA.
HOGENOMiHOG000158129.
InParanoidiP24181.
KOiK18142.
OMAiFFGWFNA.
PhylomeDBiP24181.

Family and domain databases

Gene3Di3.30.2090.10. 2 hits.
InterProiIPR027463. AcrB_DN_DC_subdom.
IPR001036. Acrflvin-R.
IPR004764. HAE1.
[Graphical view]
PfamiPF00873. ACR_tran. 1 hit.
[Graphical view]
PRINTSiPR00702. ACRIFLAVINRP.
SUPFAMiSSF82714. SSF82714. 2 hits.
TIGRFAMsiTIGR00915. 2A0602. 1 hit.

Sequencei

Sequence statusi: Complete.

P24181-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANFFIRRPI FAWVLAIILM MAGALAILQL PVAQYPTIAP PAVSVSANYP
60 70 80 90 100
GADAQTVQDT VTQVIEQNMN GIDNLMYMSS TSDSAGSVTI TLTFQSGTDP
110 120 130 140 150
DIAQVQVQNK LQLATPLLPQ EVQQQGISVE KSSSSYLMVA GFVSDNPGTT
160 170 180 190 200
QDDISDYVAS NVKDTLSRLN GVGDVQLFGA QYAMRIWLDA DLLNKYKLTP
210 220 230 240 250
VDVINQLKVQ NDQIAAGQLG GTPALPGQQL NASIIAQTRF KNPEEFGKVT
260 270 280 290 300
LRVNSDGSVV RLKDVARVEL GGENYNVIAR INGKPAAGLG IKLATGANAL
310 320 330 340 350
DTAKAIKAKL AELQPFFPQG MKVLYPYDTT PFVQLSIHEV VKTLFEAIML
360 370 380 390 400
VFLVMYLFLQ NMRATLIPTI AVPVVLLGTF AILAAFGYSI NTLTMFGMVL
410 420 430 440 450
AIGLLVDDAI VVVENVERVM MEDKLPPKEA TEKSMSQIQG ALVGIAMVLS
460 470 480 490 500
AVFIPMAFFG GSTGAIYRQF SITIVSAMAL SVLVALILTP ALCATLLKPV
510 520 530 540 550
SAEHHENKGG FFGWFNTTFD HSVNHYTNSV GKILGSTGRY LLIYALIVAG
560 570 580 590 600
MVVLFLRLPS SFLPEEDQGV FLTMIQLPAG ATQERTQKVL DQVTDYYLKN
610 620 630 640 650
EKANVESVFT VNGFSFSGQA QNAGMAFVSL KPWEERNGDE NSAEAVIHRA
660 670 680 690 700
KMELGKIRDG FVIPFNMPAI VELGTATGFD FELIDQAGLG HDALTQARNQ
710 720 730 740 750
LLGMAAQHPA SLVSVRPNGL EDTAQFKLEV DQEKAQALGV SLSDINQTIS
760 770 780 790 800
TALGGTYVND FIDRGRVKKL YVQADAKFRM LPEDVDKLYV RSANGEMVPF
810 820 830 840 850
SAFTTSHWVY GSPRLERYNG LPSMEIQGEA APGTSSGDAM ALMENLASKL
860 870 880 890 900
PAGIGYDWTG MSYQERLSGN QAPALVAISF VVVFLCLAAL YESWSIPVSV
910 920 930 940 950
MLVVPLGIVG VLLAATLFNQ KNDVYFMVGL LTTIGLSAKN AILIVEFAKD
960 970 980 990 1000
LMEKEGKGVV EATLMAVRMR LRPILMTSLA FILGVLPLAI SNGAGSGAQN
1010 1020 1030
AVGIGVMGGM VSATLLAIFF VPVFFVVIRR CFKG
Length:1,034
Mass (Da):111,454
Last modified:July 1, 1993 - v2
Checksum:iB676E07AE5BD17B1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96848 Unassigned DNA. Translation: AAA02932.1.
X57948 Genomic DNA. Translation: CAA41017.1.
U18997 Genomic DNA. Translation: AAA58070.1.
U00096 Genomic DNA. Translation: AAC76298.1.
AP009048 Genomic DNA. Translation: BAE77307.1.
PIRiD65119.
RefSeqiNP_417732.1. NC_000913.3.
WP_001273238.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76298; AAC76298; b3266.
BAE77307; BAE77307; BAE77307.
GeneIDi947768.
KEGGiecj:JW3234.
eco:b3266.
PATRICi32121962. VBIEscCol129921_3365.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96848 Unassigned DNA. Translation: AAA02932.1.
X57948 Genomic DNA. Translation: CAA41017.1.
U18997 Genomic DNA. Translation: AAA58070.1.
U00096 Genomic DNA. Translation: AAC76298.1.
AP009048 Genomic DNA. Translation: BAE77307.1.
PIRiD65119.
RefSeqiNP_417732.1. NC_000913.3.
WP_001273238.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP24181.
SMRiP24181. Positions 1-1018.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262457. 308 interactions.
DIPiDIP-9052N.
IntActiP24181. 1 interaction.
MINTiMINT-1267966.
STRINGi511145.b3266.

Protein family/group databases

TCDBi2.A.6.2.1. the resistance-nodulation-cell division (rnd) superfamily.

Proteomic databases

PaxDbiP24181.
PRIDEiP24181.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76298; AAC76298; b3266.
BAE77307; BAE77307; BAE77307.
GeneIDi947768.
KEGGiecj:JW3234.
eco:b3266.
PATRICi32121962. VBIEscCol129921_3365.

Organism-specific databases

EchoBASEiEB0263.
EcoGeneiEG10267. acrF.

Phylogenomic databases

eggNOGiENOG4105BZS. Bacteria.
COG0841. LUCA.
HOGENOMiHOG000158129.
InParanoidiP24181.
KOiK18142.
OMAiFFGWFNA.
PhylomeDBiP24181.

Enzyme and pathway databases

BioCyciEcoCyc:ACRF-MONOMER.
ECOL316407:JW3234-MONOMER.

Miscellaneous databases

PROiP24181.

Family and domain databases

Gene3Di3.30.2090.10. 2 hits.
InterProiIPR027463. AcrB_DN_DC_subdom.
IPR001036. Acrflvin-R.
IPR004764. HAE1.
[Graphical view]
PfamiPF00873. ACR_tran. 1 hit.
[Graphical view]
PRINTSiPR00702. ACRIFLAVINRP.
SUPFAMiSSF82714. SSF82714. 2 hits.
TIGRFAMsiTIGR00915. 2A0602. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiACRF_ECOLI
AccessioniPrimary (citable) accession number: P24181
Secondary accession number(s): Q2M8U9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 1, 1993
Last modified: September 7, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.