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P24181 (ACRF_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acriflavine resistance protein F
Alternative name(s):
Protein EnvD
Gene names
Name:acrF
Synonyms:envD
Ordered Locus Names:b3266, JW3234
OrganismEscherichia coli (strain K12)
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length1034 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in cell envelope formation. Is produced in extremely low amounts.

Subcellular location

Cell inner membrane; Multi-pass membrane protein Ref.6.

Sequence similarities

Belongs to the AcrB/AcrD/AcrF (TC 2.A.6) family. [View classification]

Ontologies

Keywords
   Biological processCell cycle
Cell division
Transport
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processcell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

cell division

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiontransporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10341034Acriflavine resistance protein F
PRO_0000161813

Regions

Topological domain1 – 99Cytoplasmic By similarity
Transmembrane10 – 2819Helical; Name=1; By similarity
Topological domain29 – 339311Periplasmic By similarity
Transmembrane340 – 35920Helical; Name=2; By similarity
Topological domain360 – 3656Cytoplasmic By similarity
Transmembrane366 – 38520Helical; Name=3; By similarity
Topological domain386 – 3916Periplasmic By similarity
Transmembrane392 – 41322Helical; Name=4; By similarity
Topological domain414 – 44128Cytoplasmic By similarity
Transmembrane442 – 46019Helical; Name=5; By similarity
Topological domain461 – 47313Periplasmic By similarity
Transmembrane474 – 49623Helical; Name=6; By similarity
Topological domain497 – 53741Cytoplasmic By similarity
Transmembrane538 – 55619Helical; Name=7; By similarity
Topological domain557 – 871315Periplasmic By similarity
Transmembrane872 – 89120Helical; Name=8; By similarity
Topological domain892 – 8976Cytoplasmic By similarity
Transmembrane898 – 91720Helical; Name=9; By similarity
Topological domain918 – 9236Periplasmic By similarity
Transmembrane924 – 94522Helical; Name=10; By similarity
Topological domain946 – 97328Cytoplasmic By similarity
Transmembrane974 – 99219Helical; Name=11; By similarity
Topological domain993 – 100513Periplasmic By similarity
Transmembrane1006 – 102823Helical; Name=12; By similarity
Topological domain1029 – 10346Cytoplasmic By similarity

Sequences

Sequence LengthMass (Da)Tools
P24181 [UniParc].

Last modified July 1, 1993. Version 2.
Checksum: B676E07AE5BD17B1

FASTA1,034111,454
        10         20         30         40         50         60 
MANFFIRRPI FAWVLAIILM MAGALAILQL PVAQYPTIAP PAVSVSANYP GADAQTVQDT 

        70         80         90        100        110        120 
VTQVIEQNMN GIDNLMYMSS TSDSAGSVTI TLTFQSGTDP DIAQVQVQNK LQLATPLLPQ 

       130        140        150        160        170        180 
EVQQQGISVE KSSSSYLMVA GFVSDNPGTT QDDISDYVAS NVKDTLSRLN GVGDVQLFGA 

       190        200        210        220        230        240 
QYAMRIWLDA DLLNKYKLTP VDVINQLKVQ NDQIAAGQLG GTPALPGQQL NASIIAQTRF 

       250        260        270        280        290        300 
KNPEEFGKVT LRVNSDGSVV RLKDVARVEL GGENYNVIAR INGKPAAGLG IKLATGANAL 

       310        320        330        340        350        360 
DTAKAIKAKL AELQPFFPQG MKVLYPYDTT PFVQLSIHEV VKTLFEAIML VFLVMYLFLQ 

       370        380        390        400        410        420 
NMRATLIPTI AVPVVLLGTF AILAAFGYSI NTLTMFGMVL AIGLLVDDAI VVVENVERVM 

       430        440        450        460        470        480 
MEDKLPPKEA TEKSMSQIQG ALVGIAMVLS AVFIPMAFFG GSTGAIYRQF SITIVSAMAL 

       490        500        510        520        530        540 
SVLVALILTP ALCATLLKPV SAEHHENKGG FFGWFNTTFD HSVNHYTNSV GKILGSTGRY 

       550        560        570        580        590        600 
LLIYALIVAG MVVLFLRLPS SFLPEEDQGV FLTMIQLPAG ATQERTQKVL DQVTDYYLKN 

       610        620        630        640        650        660 
EKANVESVFT VNGFSFSGQA QNAGMAFVSL KPWEERNGDE NSAEAVIHRA KMELGKIRDG 

       670        680        690        700        710        720 
FVIPFNMPAI VELGTATGFD FELIDQAGLG HDALTQARNQ LLGMAAQHPA SLVSVRPNGL 

       730        740        750        760        770        780 
EDTAQFKLEV DQEKAQALGV SLSDINQTIS TALGGTYVND FIDRGRVKKL YVQADAKFRM 

       790        800        810        820        830        840 
LPEDVDKLYV RSANGEMVPF SAFTTSHWVY GSPRLERYNG LPSMEIQGEA APGTSSGDAM 

       850        860        870        880        890        900 
ALMENLASKL PAGIGYDWTG MSYQERLSGN QAPALVAISF VVVFLCLAAL YESWSIPVSV 

       910        920        930        940        950        960 
MLVVPLGIVG VLLAATLFNQ KNDVYFMVGL LTTIGLSAKN AILIVEFAKD LMEKEGKGVV 

       970        980        990       1000       1010       1020 
EATLMAVRMR LRPILMTSLA FILGVLPLAI SNGAGSGAQN AVGIGVMGGM VSATLLAIFF 

      1030 
VPVFFVVIRR CFKG

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the acrEF operon from Escherichia coli."
Xu J., Nilles M.L., Bertrand K.P.
Submitted (MAY-1993) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Molecular analysis and nucleotide sequence of the envCD operon of Escherichia coli."
Klein J.R., Henrich B., Plapp R.
Mol. Gen. Genet. 230:230-240(1991) [PubMed: 1720861] [Abstract]
Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"Molecular cloning and characterization of acrA and acrE genes of Escherichia coli."
Ma D., Cook D.N., Alberti M., Pon N.G., Nikaido H., Hearst J.E.
J. Bacteriol. 175:6299-6313(1993) [PubMed: 8407802] [Abstract]
Cited for: SEQUENCE REVISION.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract]
Cited for: SUBCELLULAR LOCATION.
Strain: K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M96848 Unassigned DNA. Translation: AAA02932.1.
X57948 Genomic DNA. Translation: CAA41017.1.
U18997 Genomic DNA. Translation: AAA58070.1.
U00096 Genomic DNA. Translation: AAC76298.1.
AP009048 Genomic DNA. Translation: BAE77307.1.
PIRD65119.
RefSeqNP_417732.1. NC_000913.2.

3D structure databases

ProteinModelPortalP24181.
SMRP24181. Positions 1-1018.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-9052N.
IntActP24181. 1 interaction.
MINTMINT-1267966.

Protein family/group databases

TCDB2.A.6.2.1. resistance-nodulation-cell division (RND) superfamily.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBESCT00000002266; EBESCP00000002266; EBESCG00000001854.
EBESCT00000015529; EBESCP00000014820; EBESCG00000014589.
GeneID947768.
GenomeReviewsGene locus JW3234 in contig AP009048_GR.
Gene locus b3266 in contig U00096_GR.
KEGGecj:JW3234.
eco:b3266.
PATRIC32121962. VBIEscCol129921_3365.

Organism-specific databases

EchoBASEEB0263.
EcoGeneEG10267. acrF.

Phylogenomic databases

eggNOGCOG0841.
GeneTreeEBGT00050000009201.
HOGENOMHBG293864.
OMAMMERLAN.
PhylomeDBP24181.
ProtClustDBCLSK2502648.

Enzyme and pathway databases

BioCycEcoCyc:ACRF-MONOMER.

Gene expression databases

GenevestigatorP24181.

Family and domain databases

InterProIPR001036. Acrflvin-R.
IPR004764. HAE1.
[Graphical view]
KOK03296.
PfamPF00873. ACR_tran. 1 hit.
[Graphical view]
PRINTSPR00702. ACRIFLAVINRP.
TIGRFAMsTIGR00915. 2A0602. 1 hit.
ProtoNetSearch...

Entry information

Entry nameACRF_ECOLI
AccessionPrimary (citable) accession number: P24181
Secondary accession number(s): Q2M8U9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 1, 1993
Last modified: January 25, 2012
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents