Acriflavine resistance protein F - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P24181 (ACRF_ECOLI)

Last modified June 16, 2009. Version 79. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Acriflavine resistance protein F
Alternative name(s):
Protein envD

Gene names

Name:	acrF
Synonyms:	envD
Ordered Locus Names:	b3266, JW3234

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	1034 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	Involved in cell envelope formation. Is produced in extremely low amounts.
Subcellular location	Cell inner membrane; Multi-pass membrane protein. Ref.6
Sequence similarities	Belongs to the acrB/acrD/acrF (TC 2.A.6) family. [View classification]

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Ontologies

Keywords
Biological process	Cell cycle Cell division Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1034

1034

Acriflavine resistance protein F

PRO_0000161813

Regions

Topological domain

1 – 9

Cytoplasmic By similarity

Transmembrane

10 – 28

1 By similarity

Topological domain

29 – 339

311

Periplasmic By similarity

Transmembrane

340 – 359

2 By similarity

Topological domain

360 – 365

Cytoplasmic By similarity

Transmembrane

366 – 385

3 By similarity

Topological domain

386 – 391

Periplasmic By similarity

Transmembrane

392 – 413

4 By similarity

Topological domain

414 – 441

Cytoplasmic By similarity

Transmembrane

442 – 460

5 By similarity

Topological domain

461 – 473

Periplasmic By similarity

Transmembrane

474 – 496

6 By similarity

Topological domain

497 – 537

Cytoplasmic By similarity

Transmembrane

538 – 556

7 By similarity

Topological domain

557 – 871

315

Periplasmic By similarity

Transmembrane

872 – 891

8 By similarity

Topological domain

892 – 897

Cytoplasmic By similarity

Transmembrane

898 – 917

9 By similarity

Topological domain

918 – 923

Periplasmic By similarity

Transmembrane

924 – 945

10 By similarity

Topological domain

946 – 973

Cytoplasmic By similarity

Transmembrane

974 – 992

11 By similarity

Topological domain

993 – 1005

Periplasmic By similarity

Transmembrane

1006 – 1028

12 By similarity

Topological domain

1029 – 1034

Cytoplasmic By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

P24181-1 [UniParc].

Last modified July 1, 1993. Version 2.
Checksum: B676E07AE5BD17B1
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FASTA

1,034

111,454

        10         20         30         40         50         60 
MANFFIRRPI FAWVLAIILM MAGALAILQL PVAQYPTIAP PAVSVSANYP GADAQTVQDT 

        70         80         90        100        110        120 
VTQVIEQNMN GIDNLMYMSS TSDSAGSVTI TLTFQSGTDP DIAQVQVQNK LQLATPLLPQ 

       130        140        150        160        170        180 
EVQQQGISVE KSSSSYLMVA GFVSDNPGTT QDDISDYVAS NVKDTLSRLN GVGDVQLFGA 

       190        200        210        220        230        240 
QYAMRIWLDA DLLNKYKLTP VDVINQLKVQ NDQIAAGQLG GTPALPGQQL NASIIAQTRF 

       250        260        270        280        290        300 
KNPEEFGKVT LRVNSDGSVV RLKDVARVEL GGENYNVIAR INGKPAAGLG IKLATGANAL 

       310        320        330        340        350        360 
DTAKAIKAKL AELQPFFPQG MKVLYPYDTT PFVQLSIHEV VKTLFEAIML VFLVMYLFLQ 

       370        380        390        400        410        420 
NMRATLIPTI AVPVVLLGTF AILAAFGYSI NTLTMFGMVL AIGLLVDDAI VVVENVERVM 

       430        440        450        460        470        480 
MEDKLPPKEA TEKSMSQIQG ALVGIAMVLS AVFIPMAFFG GSTGAIYRQF SITIVSAMAL 

       490        500        510        520        530        540 
SVLVALILTP ALCATLLKPV SAEHHENKGG FFGWFNTTFD HSVNHYTNSV GKILGSTGRY 

       550        560        570        580        590        600 
LLIYALIVAG MVVLFLRLPS SFLPEEDQGV FLTMIQLPAG ATQERTQKVL DQVTDYYLKN 

       610        620        630        640        650        660 
EKANVESVFT VNGFSFSGQA QNAGMAFVSL KPWEERNGDE NSAEAVIHRA KMELGKIRDG 

       670        680        690        700        710        720 
FVIPFNMPAI VELGTATGFD FELIDQAGLG HDALTQARNQ LLGMAAQHPA SLVSVRPNGL 

       730        740        750        760        770        780 
EDTAQFKLEV DQEKAQALGV SLSDINQTIS TALGGTYVND FIDRGRVKKL YVQADAKFRM 

       790        800        810        820        830        840 
LPEDVDKLYV RSANGEMVPF SAFTTSHWVY GSPRLERYNG LPSMEIQGEA APGTSSGDAM 

       850        860        870        880        890        900 
ALMENLASKL PAGIGYDWTG MSYQERLSGN QAPALVAISF VVVFLCLAAL YESWSIPVSV 

       910        920        930        940        950        960 
MLVVPLGIVG VLLAATLFNQ KNDVYFMVGL LTTIGLSAKN AILIVEFAKD LMEKEGKGVV 

       970        980        990       1000       1010       1020 
EATLMAVRMR LRPILMTSLA FILGVLPLAI SNGAGSGAQN AVGIGVMGGM VSATLLAIFF 

      1030 
VPVFFVVIRR CFKG

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the acrEF operon from Escherichia coli." Xu J., Nilles M.L., Bertrand K.P. Submitted (MAY-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Molecular analysis and nucleotide sequence of the envCD operon of Escherichia coli." Klein J.R., Henrich B., Plapp R. Mol. Gen. Genet. 230:230-240(1991) [PubMed: 1720861] [Abstract] Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"Molecular cloning and characterization of acrA and acrE genes of Escherichia coli." Ma D., Cook D.N., Alberti M., Pon N.G., Nikaido H., Hearst J.E. J. Bacteriol. 175:6299-6313(1993) [PubMed: 8407802] [Abstract] Cited for: SEQUENCE REVISION.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.

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Cross-references

Sequence databases
	M96848 Unassigned DNA. Translation: AAA02932.1. X57948 Genomic DNA. Translation: CAA41017.1. U18997 Genomic DNA. Translation: AAA58070.1. U00096 Genomic DNA. Translation: AAC76298.1. AP009048 Genomic DNA. Translation: BAE77307.1.
PIR	D65119.
RefSeq	AP_003806.1. NP_417732.1.
3D structure databases
HSSP	HSSP built from PDB template 1IWG based on UniProtKB P31224.
SMR	P24181. Positions 1-1018.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:9052N.
Protein family/group databases
TCDB	2.A.6.2.1. resistance-nodulation-cell division (RND) superfamily.
Genome annotation databases
GeneID	947768.
GenomeReviews	Gene locus JW3234 in contig AP009048_GR. Gene locus b3266 in contig U00096_GR.
KEGG	ecj:JW3234. eco:b3266.
Organism-specific databases
EchoBASE	EB0263.
EcoGene	EG10267. acrF.
CMR	Search...
Phylogenomic databases
HOGENOM	P24181.
OMA	P24181. ELIDQAN.
Enzyme and pathway databases
BioCyc	EcoCyc:ACRF-MON.
Family and domain databases
InterPro	IPR001036. Acrflvin-R. IPR004764. HAE1. [Graphical view]
Pfam	PF00873. ACR_tran. 1 hit. [Graphical view]
PRINTS	PR00702. ACRIFLAVINRP.
TIGRFAMs	TIGR00915. 2A0602. 1 hit.
ProtoNet	Search...

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Entry information

Entry name

ACRF_ECOLI

Accession

Primary (citable) accession number: P24181
Secondary accession number(s): Q2M8U9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents