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P24180

- ACRE_ECOLI

UniProt

P24180 - ACRE_ECOLI

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Protein

Multidrug export protein AcrE

Gene

acrE

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Part of the tripartite efflux system AcrEF-TolC. Involved in the efflux of indole and organic solvents.2 Publications

GO - Biological processi

  1. response to drug Source: EcoliWiki
  2. transmembrane transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG10266-MONOMER.
ECOL316407:JW3233-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug export protein AcrE
Alternative name(s):
Acriflavine resistance protein E
Protein EnvC
Gene namesi
Name:acrE
Synonyms:envC
Ordered Locus Names:b3265, JW3233
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10266. acrE.

Subcellular locationi

Cell inner membrane 1 Publication; Lipid-anchor 1 Publication

GO - Cellular componenti

  1. intrinsic component of plasma membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323PROSITE-ProRule annotationAdd
BLAST
Chaini24 – 385362Multidrug export protein AcrEPRO_0000018688Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi24 – 241N-palmitoyl cysteinePROSITE-ProRule annotation
Lipidationi24 – 241S-diacylglycerol cysteinePROSITE-ProRule annotation

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP24180.
PRIDEiP24180.

Expressioni

Inductioni

Induced by LeuO, part of the acrEF operon.1 Publication

Gene expression databases

GenevestigatoriP24180.

Interactioni

Subunit structurei

Part of the tripartite efflux system AcrEF-TolC, which is composed of an inner membrane transporter, AcrF, a periplasmic membrane fusion protein, AcrE, and an outer membrane component, TolC. The complex forms a large protein conduit and can translocate molecules across both the inner and outer membranes (Probable).1 Publication

Protein-protein interaction databases

STRINGi511145.b3265.

Structurei

3D structure databases

ProteinModelPortaliP24180.
SMRiP24180. Positions 37-373.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0845.
HOGENOMiHOG000158247.
InParanoidiP24180.
KOiK18141.
OMAiAVDNAFI.
OrthoDBiEOG6GJBSS.
PhylomeDBiP24180.

Family and domain databases

InterProiIPR006143. RND_pump_MFP.
[Graphical view]
PfamiPF00529. HlyD. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01730. RND_mfp. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24180-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTKHARFFLL PSFILISAAL IAGCNDKGEE KAHVGEPQVT VHIVKTAPLE
60 70 80 90 100
VKTELPGRTN AYRIAEVRPQ VSGIVLNRNF TEGSDVQAGQ SLYQIDPATY
110 120 130 140 150
QANYDSAKGE LAKSEAAAAI AHLTVKRYVP LVGTKYISQQ EYDQAIADAR
160 170 180 190 200
QADAAVIAAK ATVESARINL AYTKVTAPIS GRIGKSTVTE GALVTNGQTT
210 220 230 240 250
ELATVQQLDP IYVDVTQSSN DFMRLKQSVE QGNLHKENAT SNVELVMENG
260 270 280 290 300
QTYPLKGTLQ FSDVTVDEST GSITLRAVFP NPQHTLLPGM FVRARIDEGV
310 320 330 340 350
QPDAILIPQQ GVSRTPRGDA TVLIVNDKSQ VEARPVVASQ AIGDKWLISE
360 370 380
GLKSGDQVIV SGLQKARPGE QVKATTDTPA DTASK
Length:385
Mass (Da):41,318
Last modified:July 1, 1993 - v2
Checksum:iEDBF82C853CF42A9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti191 – 21222GALVT…LDPIY → ALLSLMGKRLNWRLSSSSIL ST(PubMed:1720861)CuratedAdd
BLAST
Sequence conflicti313 – 3142SR → T(PubMed:1720861)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96848 Unassigned DNA. Translation: AAA02931.1.
X57948 Genomic DNA. Translation: CAA41016.1.
U18997 Genomic DNA. Translation: AAA58069.1.
U00096 Genomic DNA. Translation: AAC76297.1.
AP009048 Genomic DNA. Translation: BAE77306.1.
PIRiC65119.
RefSeqiNP_417731.1. NC_000913.3.
YP_491447.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76297; AAC76297; b3265.
BAE77306; BAE77306; BAE77306.
GeneIDi12932959.
947706.
KEGGiecj:Y75_p3183.
eco:b3265.
PATRICi32121960. VBIEscCol129921_3364.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96848 Unassigned DNA. Translation: AAA02931.1 .
X57948 Genomic DNA. Translation: CAA41016.1 .
U18997 Genomic DNA. Translation: AAA58069.1 .
U00096 Genomic DNA. Translation: AAC76297.1 .
AP009048 Genomic DNA. Translation: BAE77306.1 .
PIRi C65119.
RefSeqi NP_417731.1. NC_000913.3.
YP_491447.1. NC_007779.1.

3D structure databases

ProteinModelPortali P24180.
SMRi P24180. Positions 37-373.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 511145.b3265.

Proteomic databases

PaxDbi P24180.
PRIDEi P24180.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76297 ; AAC76297 ; b3265 .
BAE77306 ; BAE77306 ; BAE77306 .
GeneIDi 12932959.
947706.
KEGGi ecj:Y75_p3183.
eco:b3265.
PATRICi 32121960. VBIEscCol129921_3364.

Organism-specific databases

EchoBASEi EB0262.
EcoGenei EG10266. acrE.

Phylogenomic databases

eggNOGi COG0845.
HOGENOMi HOG000158247.
InParanoidi P24180.
KOi K18141.
OMAi AVDNAFI.
OrthoDBi EOG6GJBSS.
PhylomeDBi P24180.

Enzyme and pathway databases

BioCyci EcoCyc:EG10266-MONOMER.
ECOL316407:JW3233-MONOMER.

Miscellaneous databases

PROi P24180.

Gene expression databases

Genevestigatori P24180.

Family and domain databases

InterProi IPR006143. RND_pump_MFP.
[Graphical view ]
Pfami PF00529. HlyD. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01730. RND_mfp. 1 hit.
PROSITEi PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the acrEF operon from Escherichia coli."
    Xu J., Bertrand K.P.
    Submitted (MAY-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Molecular analysis and nucleotide sequence of the envCD operon of Escherichia coli."
    Klein J.R., Henrich B., Plapp R.
    Mol. Gen. Genet. 230:230-240(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Molecular cloning of the envC gene of Escherichia coli."
    Klein J.R., Henrich B., Plapp R.
    Curr. Microbiol. 21:341-347(1990)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-96.
    Strain: K12.
  6. "Suppression of hypersensitivity of Escherichia coli acrB mutant to organic solvents by integrational activation of the acrEF operon with the IS1 or IS2 element."
    Kobayashi K., Tsukagoshi N., Aono R.
    J. Bacteriol. 183:2646-2653(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 291-299, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
  7. "Role of multiple efflux pumps in Escherichia coli in indole expulsion."
    Kawamura-Sato K., Shibayama K., Horii T., Iimuma Y., Arakawa Y., Ohta M.
    FEMS Microbiol. Lett. 179:345-352(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: K12.
  8. "Involvement of the leucine response transcription factor LeuO in regulation of the genes for sulfa drug efflux."
    Shimada T., Yamamoto K., Ishihama A.
    J. Bacteriol. 191:4562-4571(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: OPERON STRUCTURE, INDUCTION.
    Strain: K12 / BW25113.

Entry informationi

Entry nameiACRE_ECOLI
AccessioniPrimary (citable) accession number: P24180
Secondary accession number(s): Q2M8V0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 1, 1993
Last modified: October 29, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3