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Protein

Multidrug export protein AcrE

Gene

acrE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Part of the tripartite efflux system AcrEF-TolC. Involved in the efflux of indole and organic solvents.2 Publications

GO - Molecular functioni

GO - Biological processi

  • drug transmembrane transport Source: GO_Central
  • response to antibiotic Source: GO_Central
  • response to drug Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG10266-MONOMER.
ECOL316407:JW3233-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug export protein AcrE
Alternative name(s):
Acriflavine resistance protein E
Protein EnvC
Gene namesi
Name:acrE
Synonyms:envC
Ordered Locus Names:b3265, JW3233
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10266. acrE.

Subcellular locationi

  • Cell inner membrane 1 Publication; Lipid-anchor 1 Publication

GO - Cellular componenti

  • intrinsic component of plasma membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323PROSITE-ProRule annotationAdd
BLAST
Chaini24 – 385362Multidrug export protein AcrEPRO_0000018688Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi24 – 241N-palmitoyl cysteinePROSITE-ProRule annotation
Lipidationi24 – 241S-diacylglycerol cysteinePROSITE-ProRule annotation

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP24180.
PRIDEiP24180.

Expressioni

Inductioni

Induced by LeuO, part of the acrEF operon.1 Publication

Interactioni

Subunit structurei

Part of the tripartite efflux system AcrEF-TolC, which is composed of an inner membrane transporter, AcrF, a periplasmic membrane fusion protein, AcrE, and an outer membrane component, TolC. The complex forms a large protein conduit and can translocate molecules across both the inner and outer membranes (Probable).1 Publication

Protein-protein interaction databases

BioGridi4261953. 367 interactions.
STRINGi511145.b3265.

Structurei

3D structure databases

ProteinModelPortaliP24180.
SMRiP24180. Positions 37-373.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105C1P. Bacteria.
ENOG410XNVN. LUCA.
HOGENOMiHOG000158247.
InParanoidiP24180.
KOiK18141.
OMAiQRGGDTK.
PhylomeDBiP24180.

Family and domain databases

InterProiIPR032317. HlyD_D23.
IPR006143. RND_pump_MFP.
[Graphical view]
PfamiPF00529. HlyD. 1 hit.
PF16576. HlyD_D23. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01730. RND_mfp. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24180-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKHARFFLL PSFILISAAL IAGCNDKGEE KAHVGEPQVT VHIVKTAPLE
60 70 80 90 100
VKTELPGRTN AYRIAEVRPQ VSGIVLNRNF TEGSDVQAGQ SLYQIDPATY
110 120 130 140 150
QANYDSAKGE LAKSEAAAAI AHLTVKRYVP LVGTKYISQQ EYDQAIADAR
160 170 180 190 200
QADAAVIAAK ATVESARINL AYTKVTAPIS GRIGKSTVTE GALVTNGQTT
210 220 230 240 250
ELATVQQLDP IYVDVTQSSN DFMRLKQSVE QGNLHKENAT SNVELVMENG
260 270 280 290 300
QTYPLKGTLQ FSDVTVDEST GSITLRAVFP NPQHTLLPGM FVRARIDEGV
310 320 330 340 350
QPDAILIPQQ GVSRTPRGDA TVLIVNDKSQ VEARPVVASQ AIGDKWLISE
360 370 380
GLKSGDQVIV SGLQKARPGE QVKATTDTPA DTASK
Length:385
Mass (Da):41,318
Last modified:July 1, 1993 - v2
Checksum:iEDBF82C853CF42A9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti191 – 21222GALVT…LDPIY → ALLSLMGKRLNWRLSSSSIL ST (PubMed:1720861).CuratedAdd
BLAST
Sequence conflicti313 – 3142SR → T (PubMed:1720861).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96848 Unassigned DNA. Translation: AAA02931.1.
X57948 Genomic DNA. Translation: CAA41016.1.
U18997 Genomic DNA. Translation: AAA58069.1.
U00096 Genomic DNA. Translation: AAC76297.1.
AP009048 Genomic DNA. Translation: BAE77306.1.
PIRiC65119.
RefSeqiNP_417731.1. NC_000913.3.
WP_000160334.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76297; AAC76297; b3265.
BAE77306; BAE77306; BAE77306.
GeneIDi947706.
KEGGiecj:JW3233.
eco:b3265.
PATRICi32121960. VBIEscCol129921_3364.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96848 Unassigned DNA. Translation: AAA02931.1.
X57948 Genomic DNA. Translation: CAA41016.1.
U18997 Genomic DNA. Translation: AAA58069.1.
U00096 Genomic DNA. Translation: AAC76297.1.
AP009048 Genomic DNA. Translation: BAE77306.1.
PIRiC65119.
RefSeqiNP_417731.1. NC_000913.3.
WP_000160334.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP24180.
SMRiP24180. Positions 37-373.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261953. 367 interactions.
STRINGi511145.b3265.

Proteomic databases

PaxDbiP24180.
PRIDEiP24180.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76297; AAC76297; b3265.
BAE77306; BAE77306; BAE77306.
GeneIDi947706.
KEGGiecj:JW3233.
eco:b3265.
PATRICi32121960. VBIEscCol129921_3364.

Organism-specific databases

EchoBASEiEB0262.
EcoGeneiEG10266. acrE.

Phylogenomic databases

eggNOGiENOG4105C1P. Bacteria.
ENOG410XNVN. LUCA.
HOGENOMiHOG000158247.
InParanoidiP24180.
KOiK18141.
OMAiQRGGDTK.
PhylomeDBiP24180.

Enzyme and pathway databases

BioCyciEcoCyc:EG10266-MONOMER.
ECOL316407:JW3233-MONOMER.

Miscellaneous databases

PROiP24180.

Family and domain databases

InterProiIPR032317. HlyD_D23.
IPR006143. RND_pump_MFP.
[Graphical view]
PfamiPF00529. HlyD. 1 hit.
PF16576. HlyD_D23. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01730. RND_mfp. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACRE_ECOLI
AccessioniPrimary (citable) accession number: P24180
Secondary accession number(s): Q2M8V0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 1, 1993
Last modified: September 7, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.