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P24180

- ACRE_ECOLI

UniProt

P24180 - ACRE_ECOLI

Protein

Multidrug export protein AcrE

Gene

acrE

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Part of the tripartite efflux system AcrEF-TolC. Involved in the efflux of indole and organic solvents.2 Publications

    GO - Biological processi

    1. response to drug Source: EcoliWiki
    2. transmembrane transport Source: InterPro

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10266-MONOMER.
    ECOL316407:JW3233-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Multidrug export protein AcrE
    Alternative name(s):
    Acriflavine resistance protein E
    Protein EnvC
    Gene namesi
    Name:acrE
    Synonyms:envC
    Ordered Locus Names:b3265, JW3233
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10266. acrE.

    Subcellular locationi

    Cell inner membrane 1 Publication; Lipid-anchor 1 Publication

    GO - Cellular componenti

    1. intrinsic component of plasma membrane Source: EcoliWiki

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323PROSITE-ProRule annotationAdd
    BLAST
    Chaini24 – 385362Multidrug export protein AcrEPRO_0000018688Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi24 – 241N-palmitoyl cysteinePROSITE-ProRule annotation
    Lipidationi24 – 241S-diacylglycerol cysteinePROSITE-ProRule annotation

    Keywords - PTMi

    Lipoprotein, Palmitate

    Proteomic databases

    PaxDbiP24180.
    PRIDEiP24180.

    Expressioni

    Inductioni

    Induced by LeuO, part of the acrEF operon.1 Publication

    Gene expression databases

    GenevestigatoriP24180.

    Interactioni

    Subunit structurei

    Part of the tripartite efflux system AcrEF-TolC, which is composed of an inner membrane transporter, AcrF, a periplasmic membrane fusion protein, AcrE, and an outer membrane component, TolC. The complex forms a large protein conduit and can translocate molecules across both the inner and outer membranes Probable.1 Publication

    Protein-protein interaction databases

    STRINGi511145.b3265.

    Structurei

    3D structure databases

    ProteinModelPortaliP24180.
    SMRiP24180. Positions 37-373.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0845.
    HOGENOMiHOG000158247.
    KOiK18141.
    OMAiAVDNAFI.
    OrthoDBiEOG6GJBSS.
    PhylomeDBiP24180.

    Family and domain databases

    InterProiIPR006143. RND_pump_MFP.
    [Graphical view]
    PfamiPF00529. HlyD. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01730. RND_mfp. 1 hit.
    PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P24180-1 [UniParc]FASTAAdd to Basket

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    MTKHARFFLL PSFILISAAL IAGCNDKGEE KAHVGEPQVT VHIVKTAPLE    50
    VKTELPGRTN AYRIAEVRPQ VSGIVLNRNF TEGSDVQAGQ SLYQIDPATY 100
    QANYDSAKGE LAKSEAAAAI AHLTVKRYVP LVGTKYISQQ EYDQAIADAR 150
    QADAAVIAAK ATVESARINL AYTKVTAPIS GRIGKSTVTE GALVTNGQTT 200
    ELATVQQLDP IYVDVTQSSN DFMRLKQSVE QGNLHKENAT SNVELVMENG 250
    QTYPLKGTLQ FSDVTVDEST GSITLRAVFP NPQHTLLPGM FVRARIDEGV 300
    QPDAILIPQQ GVSRTPRGDA TVLIVNDKSQ VEARPVVASQ AIGDKWLISE 350
    GLKSGDQVIV SGLQKARPGE QVKATTDTPA DTASK 385
    Length:385
    Mass (Da):41,318
    Last modified:July 1, 1993 - v2
    Checksum:iEDBF82C853CF42A9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti191 – 21222GALVT…LDPIY → ALLSLMGKRLNWRLSSSSIL ST(PubMed:1720861)CuratedAdd
    BLAST
    Sequence conflicti313 – 3142SR → T(PubMed:1720861)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96848 Unassigned DNA. Translation: AAA02931.1.
    X57948 Genomic DNA. Translation: CAA41016.1.
    U18997 Genomic DNA. Translation: AAA58069.1.
    U00096 Genomic DNA. Translation: AAC76297.1.
    AP009048 Genomic DNA. Translation: BAE77306.1.
    PIRiC65119.
    RefSeqiNP_417731.1. NC_000913.3.
    YP_491447.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76297; AAC76297; b3265.
    BAE77306; BAE77306; BAE77306.
    GeneIDi12932959.
    947706.
    KEGGiecj:Y75_p3183.
    eco:b3265.
    PATRICi32121960. VBIEscCol129921_3364.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96848 Unassigned DNA. Translation: AAA02931.1 .
    X57948 Genomic DNA. Translation: CAA41016.1 .
    U18997 Genomic DNA. Translation: AAA58069.1 .
    U00096 Genomic DNA. Translation: AAC76297.1 .
    AP009048 Genomic DNA. Translation: BAE77306.1 .
    PIRi C65119.
    RefSeqi NP_417731.1. NC_000913.3.
    YP_491447.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P24180.
    SMRi P24180. Positions 37-373.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 511145.b3265.

    Proteomic databases

    PaxDbi P24180.
    PRIDEi P24180.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76297 ; AAC76297 ; b3265 .
    BAE77306 ; BAE77306 ; BAE77306 .
    GeneIDi 12932959.
    947706.
    KEGGi ecj:Y75_p3183.
    eco:b3265.
    PATRICi 32121960. VBIEscCol129921_3364.

    Organism-specific databases

    EchoBASEi EB0262.
    EcoGenei EG10266. acrE.

    Phylogenomic databases

    eggNOGi COG0845.
    HOGENOMi HOG000158247.
    KOi K18141.
    OMAi AVDNAFI.
    OrthoDBi EOG6GJBSS.
    PhylomeDBi P24180.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10266-MONOMER.
    ECOL316407:JW3233-MONOMER.

    Miscellaneous databases

    PROi P24180.

    Gene expression databases

    Genevestigatori P24180.

    Family and domain databases

    InterProi IPR006143. RND_pump_MFP.
    [Graphical view ]
    Pfami PF00529. HlyD. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01730. RND_mfp. 1 hit.
    PROSITEi PS51257. PROKAR_LIPOPROTEIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the acrEF operon from Escherichia coli."
      Xu J., Bertrand K.P.
      Submitted (MAY-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Molecular analysis and nucleotide sequence of the envCD operon of Escherichia coli."
      Klein J.R., Henrich B., Plapp R.
      Mol. Gen. Genet. 230:230-240(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Molecular cloning of the envC gene of Escherichia coli."
      Klein J.R., Henrich B., Plapp R.
      Curr. Microbiol. 21:341-347(1990)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-96.
      Strain: K12.
    6. "Suppression of hypersensitivity of Escherichia coli acrB mutant to organic solvents by integrational activation of the acrEF operon with the IS1 or IS2 element."
      Kobayashi K., Tsukagoshi N., Aono R.
      J. Bacteriol. 183:2646-2653(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 291-299, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
    7. "Role of multiple efflux pumps in Escherichia coli in indole expulsion."
      Kawamura-Sato K., Shibayama K., Horii T., Iimuma Y., Arakawa Y., Ohta M.
      FEMS Microbiol. Lett. 179:345-352(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: K12.
    8. "Involvement of the leucine response transcription factor LeuO in regulation of the genes for sulfa drug efflux."
      Shimada T., Yamamoto K., Ishihama A.
      J. Bacteriol. 191:4562-4571(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: OPERON STRUCTURE, INDUCTION.
      Strain: K12 / BW25113.

    Entry informationi

    Entry nameiACRE_ECOLI
    AccessioniPrimary (citable) accession number: P24180
    Secondary accession number(s): Q2M8V0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3