P24180 (ACRE_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acriflavine resistance protein E

Alternative name(s):
Protein EnvC

Gene names

Name:	acrE
Synonyms:	envC
Ordered Locus Names:	b3265, JW3233

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	385 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May affect specific membrane functions, such as septum formation during cell division, and cell membrane permeability.
Subcellular location	Cell inner membrane; Lipid-anchor Potential.
Induction	Induced by LeuO, part of the acrEF operon. Ref.6
Sequence similarities	Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family. [View classification]

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Signal
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW response to drug Inferred from sequence or structural similarity PubMed 8407802. Source: EcoliWiki transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular_component	intrinsic to plasma membrane Inferred from mutant phenotype PubMed 8407802. Source: EcoliWiki
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 385	362	Acriflavine resistance protein E		PRO_0000018688
Amino acid modifications
	Lipidation	24	1	N-palmitoyl cysteine Potential
	Lipidation	24	1	S-diacylglycerol cysteine Potential
Experimental info
	Sequence conflict	191 – 212	22	GALVT…LDPIY → ALLSLMGKRLNWRLSSSSIL ST Ref.2
	Sequence conflict	313 – 314	2	SR → T Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P24180 [UniParc]. Last modified July 1, 1993. Version 2. Checksum: EDBF82C853CF42A9	FASTA	385	41,318
	10 20 30 40 50 60 MTKHARFFLL PSFILISAAL IAGCNDKGEE KAHVGEPQVT VHIVKTAPLE VKTELPGRTN 70 80 90 100 110 120 AYRIAEVRPQ VSGIVLNRNF TEGSDVQAGQ SLYQIDPATY QANYDSAKGE LAKSEAAAAI 130 140 150 160 170 180 AHLTVKRYVP LVGTKYISQQ EYDQAIADAR QADAAVIAAK ATVESARINL AYTKVTAPIS 190 200 210 220 230 240 GRIGKSTVTE GALVTNGQTT ELATVQQLDP IYVDVTQSSN DFMRLKQSVE QGNLHKENAT 250 260 270 280 290 300 SNVELVMENG QTYPLKGTLQ FSDVTVDEST GSITLRAVFP NPQHTLLPGM FVRARIDEGV 310 320 330 340 350 360 QPDAILIPQQ GVSRTPRGDA TVLIVNDKSQ VEARPVVASQ AIGDKWLISE GLKSGDQVIV 370 380 SGLQKARPGE QVKATTDTPA DTASK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the acrEF operon from Escherichia coli." Xu J., Bertrand K.P. Submitted (MAY-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Molecular analysis and nucleotide sequence of the envCD operon of Escherichia coli." Klein J.R., Henrich B., Plapp R. Mol. Gen. Genet. 230:230-240(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Molecular cloning of the envC gene of Escherichia coli." Klein J.R., Henrich B., Plapp R. Curr. Microbiol. 21:341-347(1990) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-96. Strain: K12.
[6]	"Involvement of the leucine response transcription factor LeuO in regulation of the genes for sulfa drug efflux." Shimada T., Yamamoto K., Ishihama A. J. Bacteriol. 191:4562-4571(2009) [PubMed] [Europe PMC] [Abstract] Cited for: OPERON STRUCTURE, INDUCTION. Strain: K12 / BW25113.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M96848 Unassigned DNA. Translation: AAA02931.1. X57948 Genomic DNA. Translation: CAA41016.1. U18997 Genomic DNA. Translation: AAA58069.1. U00096 Genomic DNA. Translation: AAC76297.1. AP009048 Genomic DNA. Translation: BAE77306.1.
PIR	C65119.
RefSeq	NP_417731.1. NC_000913.2. YP_491447.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P24180.
SMR	P24180. Positions 37-373.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b3265.
Proteomic databases
PaxDb	P24180.
PRIDE	P24180.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76297; AAC76297; b3265. BAE77306; BAE77306; BAE77306.
GeneID	12932959. 947706.
KEGG	ecj:Y75_p3183. eco:b3265.
PATRIC	32121960. VBIEscCol129921_3364.
Organism-specific databases
EchoBASE	EB0262.
EcoGene	EG10266. acrE.
Phylogenomic databases
eggNOG	COG0845.
HOGENOM	HOG000158247.
KO	K03585.
OMA	GDTQAHP.
ProtClustDB	CLSK880627.
Enzyme and pathway databases
BioCyc	EcoCyc:EG10266-MONOMER. ECOL316407:JW3233-MONOMER.
Gene expression databases
Genevestigator	P24180.
Family and domain databases
InterPro	IPR006143. RND_pump_MFP. [Graphical view]
Pfam	PF00529. HlyD. 1 hit. [Graphical view]
TIGRFAMs	TIGR01730. RND_mfp. 1 hit.
PROSITE	PS51257. PROKAR_LIPOPROTEIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACRE_ECOLI

Accession

Primary (citable) accession number: P24180
Secondary accession number(s): Q2M8V0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	July 1, 1993
Last modified:	May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program