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Protein

Probable aminoglycoside efflux pump

Gene

acrD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Participates in the efflux of aminoglycosides. Confers resistance to a variety of these substances.1 Publication

GO - Molecular functioni

  • drug:proton antiporter activity Source: EcoCyc
  • drug transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • drug transmembrane transport Source: EcoCyc
  • response to drug Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:ACRD-MONOMER.
ECOL316407:JW2454-MONOMER.

Protein family/group databases

TCDBi2.A.6.2.7. the resistance-nodulation-cell division (rnd) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable aminoglycoside efflux pump
Alternative name(s):
Acriflavine resistance protein D
Gene namesi
Name:acrD
Synonyms:yffA
Ordered Locus Names:b2470, JW2454
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10014. acrD.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicBy similarity
Transmembranei10 – 2819Helical; Name=1By similarityAdd
BLAST
Topological domaini29 – 339311PeriplasmicBy similarityAdd
BLAST
Transmembranei340 – 35920Helical; Name=2By similarityAdd
BLAST
Topological domaini360 – 3656CytoplasmicBy similarity
Transmembranei366 – 38520Helical; Name=3By similarityAdd
BLAST
Topological domaini386 – 3916PeriplasmicBy similarity
Transmembranei392 – 41322Helical; Name=4By similarityAdd
BLAST
Topological domaini414 – 44128CytoplasmicBy similarityAdd
BLAST
Transmembranei442 – 46019Helical; Name=5By similarityAdd
BLAST
Topological domaini461 – 47313PeriplasmicBy similarityAdd
BLAST
Transmembranei474 – 49623Helical; Name=6By similarityAdd
BLAST
Topological domaini497 – 53741CytoplasmicBy similarityAdd
BLAST
Transmembranei538 – 55619Helical; Name=7By similarityAdd
BLAST
Topological domaini557 – 870314PeriplasmicBy similarityAdd
BLAST
Transmembranei871 – 89020Helical; Name=8By similarityAdd
BLAST
Topological domaini891 – 8966CytoplasmicBy similarity
Transmembranei897 – 91620Helical; Name=9By similarityAdd
BLAST
Topological domaini917 – 9226PeriplasmicBy similarity
Transmembranei923 – 94422Helical; Name=10By similarityAdd
BLAST
Topological domaini945 – 97127CytoplasmicBy similarityAdd
BLAST
Transmembranei972 – 99019Helical; Name=11By similarityAdd
BLAST
Topological domaini991 – 100313PeriplasmicBy similarityAdd
BLAST
Transmembranei1004 – 102623Helical; Name=12By similarityAdd
BLAST
Topological domaini1027 – 103711CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10371037Probable aminoglycoside efflux pumpPRO_0000161812Add
BLAST

Proteomic databases

PaxDbiP24177.
PRIDEiP24177.

Interactioni

Protein-protein interaction databases

BioGridi4260927. 486 interactions.
DIPiDIP-9050N.
IntActiP24177. 4 interactions.
MINTiMINT-1256458.
STRINGi511145.b2470.

Structurei

3D structure databases

ProteinModelPortaliP24177.
SMRiP24177. Positions 1-1016.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZS. Bacteria.
COG0841. LUCA.
HOGENOMiHOG000158129.
InParanoidiP24177.
KOiK18324.
OMAiHRSLRWM.
PhylomeDBiP24177.

Family and domain databases

Gene3Di3.30.2090.10. 2 hits.
InterProiIPR027463. AcrB_DN_DC_subdom.
IPR001036. Acrflvin-R.
IPR004764. HAE1.
[Graphical view]
PfamiPF00873. ACR_tran. 1 hit.
[Graphical view]
PRINTSiPR00702. ACRIFLAVINRP.
SUPFAMiSSF82714. SSF82714. 2 hits.
TIGRFAMsiTIGR00915. 2A0602. 1 hit.

Sequencei

Sequence statusi: Complete.

P24177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANFFIDRPI FAWVLAILLC LTGTLAIFSL PVEQYPDLAP PNVRVTANYP
60 70 80 90 100
GASAQTLENT VTQVIEQNMT GLDNLMYMSS QSSGTGQASV TLSFKAGTDP
110 120 130 140 150
DEAVQQVQNQ LQSAMRKLPQ AVQNQGVTVR KTGDTNILTI AFVSTDGSMD
160 170 180 190 200
KQDIADYVAS NIQDPLSRVN GVGDIDAYGS QYSMRIWLDP AKLNSFQMTA
210 220 230 240 250
KDVTDAIESQ NAQIAVGQLG GTPSVDKQAL NATINAQSLL QTPEQFRDIT
260 270 280 290 300
LRVNQDGSEV RLGDVATVEM GAEKYDYLSR FNGKPASGLG VKLASGANEM
310 320 330 340 350
ATAELVLNRL DELAQYFPHG LEYKVAYETT SFVKASIEDV VKTLLEAIAL
360 370 380 390 400
VFLVMYLFLQ NFRATLIPTI AVPVVLMGTF SVLYAFGYSV NTLTMFAMVL
410 420 430 440 450
AIGLLVDDAI VVVENVERIM SEEGLTPREA TRKSMGQIQG ALVGIAMVLS
460 470 480 490 500
AVFVPMAFFG GTTGAIYRQF SITIVAAMVL SVLVAMILTP ALCATLLKPL
510 520 530 540 550
KKGEHHGQKG FFAWFNQMFN RNAERYEKGV AKILHRSLRW IVIYVLLLGG
560 570 580 590 600
MVFLFLRLPT SFLPLEDRGM FTTSVQLPSG STQQQTLKVV EQIEKYYFTH
610 620 630 640 650
EKDNIMSVFA TVGSGPGGNG QNVARMFIRL KDWSERDSKT GTSFAIIERA
660 670 680 690 700
TKAFNQIKEA RVIASSPPAI SGLGSSAGFD MELQDHAGAG HDALMAARNQ
710 720 730 740 750
LLALAAENPE LTRVRHNGLD DSPQLQIDID QRKAQALGVA IDDINDTLQT
760 770 780 790 800
AWGSSYVNDF MDRGRVKKVY VQAAAPYRML PDDINLWYVR NKDGGMVPFS
810 820 830 840 850
AFATSRWETG SPRLERYNGY SAVEIVGEAA PGVSTGTAMD IMESLVKQLP
860 870 880 890 900
NGFGLEWTAM SYQERLSGAQ APALYAISLL VVFLCLAALY ESWSVPFSVM
910 920 930 940 950
LVVPLGVIGA LLATWMRGLE NDVYFQVGLL TVIGLSAKNA ILIVEFANEM
960 970 980 990 1000
NQKGHDLFEA TLHACRQRLR PILMTSLAFI FGVLPMATST GAGSGGQHAV
1010 1020 1030
GTGVMGGMIS ATILAIYFVP LFFVLVRRRF PLKPRPE
Length:1,037
Mass (Da):113,047
Last modified:November 1, 1997 - v3
Checksum:i961611E1D24FD4E5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti303 – 3031A → G in AAA20584 (Ref. 1) Curated
Sequence conflicti372 – 3721V → E in AAA20584 (Ref. 1) Curated
Sequence conflicti385 – 3851A → D in AAA74741 (Ref. 2) Curated
Sequence conflicti461 – 4611G → P in AAA20584 (Ref. 1) Curated
Sequence conflicti665 – 6651S → PD in AAA20584 (Ref. 1) Curated
Sequence conflicti763 – 7631R → A in AAA20584 (Ref. 1) Curated
Sequence conflicti775 – 7751A → G in AAA20584 (Ref. 1) Curated
Sequence conflicti778 – 7781R → P in AAA20584 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12598 Genomic DNA. Translation: AAA20584.1.
U10436 Genomic DNA. Translation: AAA74741.1.
U00096 Genomic DNA. Translation: AAC75523.1.
AP009048 Genomic DNA. Translation: BAA16344.1.
X57403 Genomic DNA. Translation: CAA40663.1.
PIRiE65022.
RefSeqiNP_416965.1. NC_000913.3.
WP_001273151.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75523; AAC75523; b2470.
BAA16344; BAA16344; BAA16344.
GeneIDi945464.
KEGGiecj:JW2454.
eco:b2470.
PATRICi32120323. VBIEscCol129921_2564.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12598 Genomic DNA. Translation: AAA20584.1.
U10436 Genomic DNA. Translation: AAA74741.1.
U00096 Genomic DNA. Translation: AAC75523.1.
AP009048 Genomic DNA. Translation: BAA16344.1.
X57403 Genomic DNA. Translation: CAA40663.1.
PIRiE65022.
RefSeqiNP_416965.1. NC_000913.3.
WP_001273151.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP24177.
SMRiP24177. Positions 1-1016.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260927. 486 interactions.
DIPiDIP-9050N.
IntActiP24177. 4 interactions.
MINTiMINT-1256458.
STRINGi511145.b2470.

Protein family/group databases

TCDBi2.A.6.2.7. the resistance-nodulation-cell division (rnd) superfamily.

Proteomic databases

PaxDbiP24177.
PRIDEiP24177.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75523; AAC75523; b2470.
BAA16344; BAA16344; BAA16344.
GeneIDi945464.
KEGGiecj:JW2454.
eco:b2470.
PATRICi32120323. VBIEscCol129921_2564.

Organism-specific databases

EchoBASEiEB0014.
EcoGeneiEG10014. acrD.

Phylogenomic databases

eggNOGiENOG4105BZS. Bacteria.
COG0841. LUCA.
HOGENOMiHOG000158129.
InParanoidiP24177.
KOiK18324.
OMAiHRSLRWM.
PhylomeDBiP24177.

Enzyme and pathway databases

BioCyciEcoCyc:ACRD-MONOMER.
ECOL316407:JW2454-MONOMER.

Miscellaneous databases

PROiP24177.

Family and domain databases

Gene3Di3.30.2090.10. 2 hits.
InterProiIPR027463. AcrB_DN_DC_subdom.
IPR001036. Acrflvin-R.
IPR004764. HAE1.
[Graphical view]
PfamiPF00873. ACR_tran. 1 hit.
[Graphical view]
PRINTSiPR00702. ACRIFLAVINRP.
SUPFAMiSSF82714. SSF82714. 2 hits.
TIGRFAMsiTIGR00915. 2A0602. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiACRD_ECOLI
AccessioniPrimary (citable) accession number: P24177
Secondary accession number(s): P76971, P77178, Q46715
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.