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Protein

Probable aminoglycoside efflux pump

Gene

acrD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Participates in the efflux of aminoglycosides. Confers resistance to a variety of these substances.1 Publication

GO - Molecular functioni

  • drug:proton antiporter activity Source: EcoCyc
  • drug transmembrane transporter activity Source: EcoCyc
  • efflux transmembrane transporter activity Source: InterPro

GO - Biological processi

  • drug transmembrane transport Source: EcoCyc
  • response to drug Source: EcoCyc

Keywordsi

Biological processTransport

Enzyme and pathway databases

BioCyciEcoCyc:ACRD-MONOMER

Protein family/group databases

TCDBi2.A.6.2.7 the resistance-nodulation-cell division (rnd) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Probable aminoglycoside efflux pump
Alternative name(s):
Acriflavine resistance protein D
Gene namesi
Name:acrD
Synonyms:yffA
Ordered Locus Names:b2470, JW2454
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10014 acrD

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 9CytoplasmicBy similarity9
Transmembranei10 – 28Helical; Name=1By similarityAdd BLAST19
Topological domaini29 – 339PeriplasmicBy similarityAdd BLAST311
Transmembranei340 – 359Helical; Name=2By similarityAdd BLAST20
Topological domaini360 – 365CytoplasmicBy similarity6
Transmembranei366 – 385Helical; Name=3By similarityAdd BLAST20
Topological domaini386 – 391PeriplasmicBy similarity6
Transmembranei392 – 413Helical; Name=4By similarityAdd BLAST22
Topological domaini414 – 441CytoplasmicBy similarityAdd BLAST28
Transmembranei442 – 460Helical; Name=5By similarityAdd BLAST19
Topological domaini461 – 473PeriplasmicBy similarityAdd BLAST13
Transmembranei474 – 496Helical; Name=6By similarityAdd BLAST23
Topological domaini497 – 537CytoplasmicBy similarityAdd BLAST41
Transmembranei538 – 556Helical; Name=7By similarityAdd BLAST19
Topological domaini557 – 870PeriplasmicBy similarityAdd BLAST314
Transmembranei871 – 890Helical; Name=8By similarityAdd BLAST20
Topological domaini891 – 896CytoplasmicBy similarity6
Transmembranei897 – 916Helical; Name=9By similarityAdd BLAST20
Topological domaini917 – 922PeriplasmicBy similarity6
Transmembranei923 – 944Helical; Name=10By similarityAdd BLAST22
Topological domaini945 – 971CytoplasmicBy similarityAdd BLAST27
Transmembranei972 – 990Helical; Name=11By similarityAdd BLAST19
Topological domaini991 – 1003PeriplasmicBy similarityAdd BLAST13
Transmembranei1004 – 1026Helical; Name=12By similarityAdd BLAST23
Topological domaini1027 – 1037CytoplasmicBy similarityAdd BLAST11

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001618121 – 1037Probable aminoglycoside efflux pumpAdd BLAST1037

Proteomic databases

PaxDbiP24177
PRIDEiP24177

Interactioni

Protein-protein interaction databases

BioGridi4260927, 488 interactors
DIPiDIP-9050N
IntActiP24177, 4 interactors
STRINGi316385.ECDH10B_2635

Structurei

3D structure databases

ProteinModelPortaliP24177
SMRiP24177
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZS Bacteria
COG0841 LUCA
HOGENOMiHOG000158129
InParanoidiP24177
KOiK18324
OMAiLAGYEIM
PhylomeDBiP24177

Family and domain databases

Gene3Di3.30.2090.10, 2 hits
InterProiView protein in InterPro
IPR027463 AcrB_DN_DC_subdom
IPR001036 Acrflvin-R
IPR004764 HAE1
PANTHERiPTHR32063 PTHR32063, 1 hit
PfamiView protein in Pfam
PF00873 ACR_tran, 1 hit
PRINTSiPR00702 ACRIFLAVINRP
SUPFAMiSSF82714 SSF82714, 2 hits
TIGRFAMsiTIGR00915 2A0602, 1 hit

Sequencei

Sequence statusi: Complete.

P24177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANFFIDRPI FAWVLAILLC LTGTLAIFSL PVEQYPDLAP PNVRVTANYP
60 70 80 90 100
GASAQTLENT VTQVIEQNMT GLDNLMYMSS QSSGTGQASV TLSFKAGTDP
110 120 130 140 150
DEAVQQVQNQ LQSAMRKLPQ AVQNQGVTVR KTGDTNILTI AFVSTDGSMD
160 170 180 190 200
KQDIADYVAS NIQDPLSRVN GVGDIDAYGS QYSMRIWLDP AKLNSFQMTA
210 220 230 240 250
KDVTDAIESQ NAQIAVGQLG GTPSVDKQAL NATINAQSLL QTPEQFRDIT
260 270 280 290 300
LRVNQDGSEV RLGDVATVEM GAEKYDYLSR FNGKPASGLG VKLASGANEM
310 320 330 340 350
ATAELVLNRL DELAQYFPHG LEYKVAYETT SFVKASIEDV VKTLLEAIAL
360 370 380 390 400
VFLVMYLFLQ NFRATLIPTI AVPVVLMGTF SVLYAFGYSV NTLTMFAMVL
410 420 430 440 450
AIGLLVDDAI VVVENVERIM SEEGLTPREA TRKSMGQIQG ALVGIAMVLS
460 470 480 490 500
AVFVPMAFFG GTTGAIYRQF SITIVAAMVL SVLVAMILTP ALCATLLKPL
510 520 530 540 550
KKGEHHGQKG FFAWFNQMFN RNAERYEKGV AKILHRSLRW IVIYVLLLGG
560 570 580 590 600
MVFLFLRLPT SFLPLEDRGM FTTSVQLPSG STQQQTLKVV EQIEKYYFTH
610 620 630 640 650
EKDNIMSVFA TVGSGPGGNG QNVARMFIRL KDWSERDSKT GTSFAIIERA
660 670 680 690 700
TKAFNQIKEA RVIASSPPAI SGLGSSAGFD MELQDHAGAG HDALMAARNQ
710 720 730 740 750
LLALAAENPE LTRVRHNGLD DSPQLQIDID QRKAQALGVA IDDINDTLQT
760 770 780 790 800
AWGSSYVNDF MDRGRVKKVY VQAAAPYRML PDDINLWYVR NKDGGMVPFS
810 820 830 840 850
AFATSRWETG SPRLERYNGY SAVEIVGEAA PGVSTGTAMD IMESLVKQLP
860 870 880 890 900
NGFGLEWTAM SYQERLSGAQ APALYAISLL VVFLCLAALY ESWSVPFSVM
910 920 930 940 950
LVVPLGVIGA LLATWMRGLE NDVYFQVGLL TVIGLSAKNA ILIVEFANEM
960 970 980 990 1000
NQKGHDLFEA TLHACRQRLR PILMTSLAFI FGVLPMATST GAGSGGQHAV
1010 1020 1030
GTGVMGGMIS ATILAIYFVP LFFVLVRRRF PLKPRPE
Length:1,037
Mass (Da):113,047
Last modified:November 1, 1997 - v3
Checksum:i961611E1D24FD4E5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti303A → G in AAA20584 (Ref. 1) Curated1
Sequence conflicti372V → E in AAA20584 (Ref. 1) Curated1
Sequence conflicti385A → D in AAA74741 (Ref. 2) Curated1
Sequence conflicti461G → P in AAA20584 (Ref. 1) Curated1
Sequence conflicti665S → PD in AAA20584 (Ref. 1) Curated1
Sequence conflicti763R → A in AAA20584 (Ref. 1) Curated1
Sequence conflicti775A → G in AAA20584 (Ref. 1) Curated1
Sequence conflicti778R → P in AAA20584 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12598 Genomic DNA Translation: AAA20584.1
U10436 Genomic DNA Translation: AAA74741.1
U00096 Genomic DNA Translation: AAC75523.1
AP009048 Genomic DNA Translation: BAA16344.1
X57403 Genomic DNA Translation: CAA40663.1
PIRiE65022
RefSeqiNP_416965.1, NC_000913.3
WP_001273151.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75523; AAC75523; b2470
BAA16344; BAA16344; BAA16344
GeneIDi945464
KEGGiecj:JW2454
eco:b2470
PATRICifig|1411691.4.peg.4270

Similar proteinsi

Entry informationi

Entry nameiACRD_ECOLI
AccessioniPrimary (citable) accession number: P24177
Secondary accession number(s): P76971, P77178, Q46715
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 140 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health