Probable aminoglycoside efflux pump - Escherichia coli (strain K12)

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P24177 (ACRD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 98. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Probable aminoglycoside efflux pump

Alternative name(s):
Acriflavine resistance protein D

Gene names

Name:	acrD
Synonyms:	yffA
Ordered Locus Names:	b2470, JW2454

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	1037 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Participates in the efflux of aminoglycosides. Confers resistance to a variety of these substances. Ref.7
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.8.
Sequence similarities	Belongs to the AcrB/AcrD/AcrF (TC 2.A.6) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	drug:hydrogen antiporter activity Inferred from direct assay. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1037

1037

Probable aminoglycoside efflux pump

PRO_0000161812

Regions

Topological domain

1 – 9

Cytoplasmic By similarity

Transmembrane

10 – 28

Helical; Name=1; By similarity

Topological domain

29 – 339

311

Periplasmic By similarity

Transmembrane

340 – 359

Helical; Name=2; By similarity

Topological domain

360 – 365

Cytoplasmic By similarity

Transmembrane

366 – 385

Helical; Name=3; By similarity

Topological domain

386 – 391

Periplasmic By similarity

Transmembrane

392 – 413

Helical; Name=4; By similarity

Topological domain

414 – 441

Cytoplasmic By similarity

Transmembrane

442 – 460

Helical; Name=5; By similarity

Topological domain

461 – 473

Periplasmic By similarity

Transmembrane

474 – 496

Helical; Name=6; By similarity

Topological domain

497 – 537

Cytoplasmic By similarity

Transmembrane

538 – 556

Helical; Name=7; By similarity

Topological domain

557 – 870

314

Periplasmic By similarity

Transmembrane

871 – 890

Helical; Name=8; By similarity

Topological domain

891 – 896

Cytoplasmic By similarity

Transmembrane

897 – 916

Helical; Name=9; By similarity

Topological domain

917 – 922

Periplasmic By similarity

Transmembrane

923 – 944

Helical; Name=10; By similarity

Topological domain

945 – 971

Cytoplasmic By similarity

Transmembrane

972 – 990

Helical; Name=11; By similarity

Topological domain

991 – 1003

Periplasmic By similarity

Transmembrane

1004 – 1026

Helical; Name=12; By similarity

Topological domain

1027 – 1037

Cytoplasmic By similarity

Experimental info

Sequence conflict

303

A → G in AAA20584. Ref.1

Sequence conflict

372

V → E in AAA20584. Ref.1

Sequence conflict

385

A → D in AAA74741. Ref.2

Sequence conflict

461

G → P in AAA20584. Ref.1

Sequence conflict

665

S → PD in AAA20584. Ref.1

Sequence conflict

763

R → A in AAA20584. Ref.1

Sequence conflict

775

A → G in AAA20584. Ref.1

Sequence conflict

778

R → P in AAA20584. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P24177 [UniParc].

Last modified November 1, 1997. Version 3.
Checksum: 961611E1D24FD4E5
Show »

FASTA

1,037

113,047

        10         20         30         40         50         60 
MANFFIDRPI FAWVLAILLC LTGTLAIFSL PVEQYPDLAP PNVRVTANYP GASAQTLENT 

        70         80         90        100        110        120 
VTQVIEQNMT GLDNLMYMSS QSSGTGQASV TLSFKAGTDP DEAVQQVQNQ LQSAMRKLPQ 

       130        140        150        160        170        180 
AVQNQGVTVR KTGDTNILTI AFVSTDGSMD KQDIADYVAS NIQDPLSRVN GVGDIDAYGS 

       190        200        210        220        230        240 
QYSMRIWLDP AKLNSFQMTA KDVTDAIESQ NAQIAVGQLG GTPSVDKQAL NATINAQSLL 

       250        260        270        280        290        300 
QTPEQFRDIT LRVNQDGSEV RLGDVATVEM GAEKYDYLSR FNGKPASGLG VKLASGANEM 

       310        320        330        340        350        360 
ATAELVLNRL DELAQYFPHG LEYKVAYETT SFVKASIEDV VKTLLEAIAL VFLVMYLFLQ 

       370        380        390        400        410        420 
NFRATLIPTI AVPVVLMGTF SVLYAFGYSV NTLTMFAMVL AIGLLVDDAI VVVENVERIM 

       430        440        450        460        470        480 
SEEGLTPREA TRKSMGQIQG ALVGIAMVLS AVFVPMAFFG GTTGAIYRQF SITIVAAMVL 

       490        500        510        520        530        540 
SVLVAMILTP ALCATLLKPL KKGEHHGQKG FFAWFNQMFN RNAERYEKGV AKILHRSLRW 

       550        560        570        580        590        600 
IVIYVLLLGG MVFLFLRLPT SFLPLEDRGM FTTSVQLPSG STQQQTLKVV EQIEKYYFTH 

       610        620        630        640        650        660 
EKDNIMSVFA TVGSGPGGNG QNVARMFIRL KDWSERDSKT GTSFAIIERA TKAFNQIKEA 

       670        680        690        700        710        720 
RVIASSPPAI SGLGSSAGFD MELQDHAGAG HDALMAARNQ LLALAAENPE LTRVRHNGLD 

       730        740        750        760        770        780 
DSPQLQIDID QRKAQALGVA IDDINDTLQT AWGSSYVNDF MDRGRVKKVY VQAAAPYRML 

       790        800        810        820        830        840 
PDDINLWYVR NKDGGMVPFS AFATSRWETG SPRLERYNGY SAVEIVGEAA PGVSTGTAMD 

       850        860        870        880        890        900 
IMESLVKQLP NGFGLEWTAM SYQERLSGAQ APALYAISLL VVFLCLAALY ESWSVPFSVM 

       910        920        930        940        950        960 
LVVPLGVIGA LLATWMRGLE NDVYFQVGLL TVIGLSAKNA ILIVEFANEM NQKGHDLFEA 

       970        980        990       1000       1010       1020 
TLHACRQRLR PILMTSLAFI FGVLPMATST GAGSGGQHAV GTGVMGGMIS ATILAIYFVP 

      1030 
LFFVLVRRRF PLKPRPE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Nilles M.L., Bertrand K.P. Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	Ma D., Cook D.N., Alberti M., Nikaido H., Hearst J.E. Submitted (AUG-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"Cloning, characterization, and expression of the dapE gene of Escherichia coli." Bouvier J., Richaud C., Higgins W., Bogler O., Stragier S. J. Bacteriol. 174:5265-5271(1992) [PubMed: 1644752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 998-1037. Strain: K12.
[7]	"AcrD of Escherichia coli is an aminoglycoside efflux pump." Rosenberg E.Y., Ma D., Nikaido H. J. Bacteriol. 182:1754-1756(2000) [PubMed: 10692383] [Abstract] Cited for: FUNCTION.
[8]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed: 15919996] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U12598 Genomic DNA. Translation: AAA20584.1. U10436 Genomic DNA. Translation: AAA74741.1. U00096 Genomic DNA. Translation: AAC75523.1. AP009048 Genomic DNA. Translation: BAA16344.1. X57403 Genomic DNA. Translation: CAA40663.1.
PIR	E65022.
RefSeq	NP_416965.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P24177.
SMR	P24177. Positions 1-1016.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-9050N.
IntAct	P24177. 4 interactions.
MINT	MINT-1256458.
Protein family/group databases
TCDB	2.A.6.2.7. resistance-nodulation-cell division (RND) superfamily.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000374; EBESCP00000000374; EBESCG00000000307. EBESCT00000016741; EBESCP00000016032; EBESCG00000015800.
GeneID	945464.
GenomeReviews	Gene locus JW2454 in contig AP009048_GR. Gene locus b2470 in contig U00096_GR.
KEGG	ecj:JW2454. eco:b2470.
PATRIC	32120323. VBIEscCol129921_2564.
Organism-specific databases
EchoBASE	EB0014.
EcoGene	EG10014. acrD.
Phylogenomic databases
eggNOG	COG0841.
GeneTree	EBGT00050000009201.
HOGENOM	HBG293864.
OMA	RRFPLKE.
PhylomeDB	P24177.
ProtClustDB	PRK10555.
Enzyme and pathway databases
BioCyc	EcoCyc:ACRD-MONOMER.
Gene expression databases
Genevestigator	P24177.
Family and domain databases
InterPro	IPR001036. Acrflvin-R. IPR004764. HAE1. [Graphical view]
KO	K03296.
Pfam	PF00873. ACR_tran. 1 hit. [Graphical view]
PRINTS	PR00702. ACRIFLAVINRP.
TIGRFAMs	TIGR00915. 2A0602. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACRD_ECOLI

Accession

Primary (citable) accession number: P24177
Secondary accession number(s): P76971, P77178, Q46715

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 98 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents