Reviewed, UniProtKB/Swiss-Prot P24174 (MANC_ECOLI)

Names and origin

Protein names

Recommended name:
    Mannose-1-phosphate guanylyltransferase
    EC=2.7.7.13
Alternative name(s):
    GDP-mannose pyrophosphorylase
      Short name=GMPP
      Short name=GMP

Gene names

Name:	manC
Synonyms:	cpsB, rfbM
Ordered Locus Names:	b2049, JW2034

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the biosynthesis of the capsular polysaccharide colanic acid.
Catalytic activity	GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 2 family.

Ontologies

Keywords
Biological process	Capsule biogenesis/degradation Lipopolysaccharide biosynthesis
Ligand	GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	colanic acid biosynthetic process Inferred from mutant phenotype. Source: UniProtKB hyperosmotic response Inferred from expression pattern. Source: UniProtKB lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mannose-1-phosphate guanylyltransferase activity Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Mannose-1-phosphate guanylyltransferase		PRO_0000194253
Experimental info
	Sequence conflict	325	1	V → L Ref.1
	Sequence conflict	325	1	V → L Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P24174-1 [UniParc]. Last modified July 19, 2003. Version 3. Checksum: 675C0EEA050B4186	FASTA	478	53,016
	10 20 30 40 50 60 MAQSKLYPVV MAGGSGSRLW PLSRVLYPKQ FLCLKGDLTM LQTTICRLNG VECESPVVIC 70 80 90 100 110 120 NEQHRFIVAE QLRQLNKLTE NIILEPAGRN TAPAIALAAL AAKRHSPESD PLMLVLAADH 130 140 150 160 170 180 VIADEDAFRA AVRNAMPYAE AGKLVTFGIV PDLPETGYGY IRRGEVSAGE QDMVAFEVAQ 190 200 210 220 230 240 FVEKPNLETA QAYVASGEYY WNSGMFLFRA GRYLEELKKY RPDILDACEK AMSAVDPDLN 250 260 270 280 290 300 FIRVDEEAFL ACPEESVDYA VMERTADAVV VPMDAGWSDV GSWSSLWEIS AHTAEGNVCH 310 320 330 340 350 360 GDVINHKTEN SYVYAESGLV TTVGVKDLVV VQTKDAVLIA DRNAVQDVKK VVEQIKADGR 370 380 390 400 410 420 HEHRVHREVY RPWGKYDSID AGDRYQVKRI TVKPGEGLSV QMHHHRAEHW VVVAGTAKVT 430 440 450 460 470 IDGDIKLLGE NESIYIPLGA THCLENPGKI PLDLIEVRSG SYLEEDDVVR FADRYGRV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for effect of random genetic drift on G+C content after lateral transfer of fucose pathway genes to Escherichia coli K-12." Aoyama K., Haase A.M., Reeves P.R. Mol. Biol. Evol. 11:829-838(1994) [PubMed: 7815923] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid." Stevenson G., Andrianopoulos K., Hobbs M., Reeves P.R. J. Bacteriol. 178:4885-4893(1996) [PubMed: 8759852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed: 9097040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"The nucleotide sequence of the phosphoglucomutase gene in E. coli." Tal R., Eichinger G., Emerick A., Wong H.C. Submitted (SEP-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 327-478.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U38473 Genomic DNA. Translation: AAC77846.1. U00096 Genomic DNA. Translation: AAC75110.1. AP009048 Genomic DNA. Translation: BAA15905.1. M77127 Genomic DNA. Translation: AAA02893.1.
PIR	H64970.
RefSeq	AP_002649.1. NP_416553.1.
3D structure databases
SMR	P24174. Positions 5-357, 256-470.
ModBase	Search...
Protein-protein interaction databases
STRING	P24174.
Genome annotation databases
GeneID	946580.
GenomeReviews	Gene locus JW2034 in contig AP009048_GR. Gene locus b2049 in contig U00096_GR.
KEGG	ecj:JW2034. eco:b2049.
Organism-specific databases
EchoBASE	EB0159.
EcoGene	EG10161. manC.
CMR	Search...
Phylogenomic databases
eggNOG	COG0836.
HOGENOM	HBG422488.
OMA	PEVTDHH.
Enzyme and pathway databases
BioCyc	EcoCyc:MANNPGUANYLTRANGDP-MONOMER. ECOL168927:B2049-MONOMER. MetaCyc:MANNPGUANYLTRANGDP-MONOMER.
Gene expression databases
Genevestigator	P24174.
Family and domain databases
InterPro	IPR011051. Cupin_RmlC_type. IPR006375. Man1P_GuaTrfase/Man6P_Isoase. IPR001538. Man6P_isomerase-2_C. IPR005835. NTP_transferase. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
Pfam	PF01050. MannoseP_isomer. 1 hit. PF00483. NTP_transferase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01479. GMP_PMI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MANC_ECOLI

Accession

Primary (citable) accession number: P24174
Secondary accession number(s): P78084

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	July 19, 2003
Last modified:	February 9, 2010
This is version 89 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)