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Protein

Mannose-1-phosphate guanylyltransferase

Gene

manC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the capsular polysaccharide colanic acid.

Catalytic activityi

GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.

Pathwayi: GDP-alpha-D-mannose biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route).
Proteins known to be involved in this subpathway in this organism are:
  1. Mannose-1-phosphate guanylyltransferase (manC)
This subpathway is part of the pathway GDP-alpha-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route), the pathway GDP-alpha-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

GO - Molecular functioni

  • GTP binding Source: UniProtKB-KW
  • mannose-1-phosphate guanylyltransferase activity Source: EcoCyc

GO - Biological processi

  • colanic acid biosynthetic process Source: EcoCyc
  • GDP-mannose biosynthetic process Source: UniProtKB-UniPathway
  • hyperosmotic response Source: EcoCyc
  • lipopolysaccharide biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Capsule biogenesis/degradation, Lipopolysaccharide biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:MANNPGUANYLTRANGDP-MONOMER.
ECOL316407:JW2034-MONOMER.
MetaCyc:MANNPGUANYLTRANGDP-MONOMER.
UniPathwayiUPA00126; UER00930.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannose-1-phosphate guanylyltransferase (EC:2.7.7.13)
Alternative name(s):
GDP-mannose pyrophosphorylase
Short name:
GMP
Short name:
GMPP
Gene namesi
Name:manC
Synonyms:cpsB, rfbM
Ordered Locus Names:b2049, JW2034
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10161. manC.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 478478Mannose-1-phosphate guanylyltransferasePRO_0000194253Add
BLAST

Proteomic databases

PaxDbiP24174.
PRIDEiP24174.

Interactioni

Protein-protein interaction databases

BioGridi4263312. 176 interactions.
IntActiP24174. 5 interactions.
STRINGi511145.b2049.

Structurei

3D structure databases

ProteinModelPortaliP24174.
SMRiP24174. Positions 9-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CJT. Bacteria.
COG0662. LUCA.
COG0836. LUCA.
HOGENOMiHOG000153549.
InParanoidiP24174.
KOiK00971.
OMAiETQIGEY.
PhylomeDBiP24174.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
3.90.550.10. 1 hit.
InterProiIPR006375. Man1P_GuaTrfase/Man6P_Isoase.
IPR001538. Man6P_isomerase-2_C.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF01050. MannoseP_isomer. 1 hit.
PF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01479. GMP_PMI. 1 hit.

Sequencei

Sequence statusi: Complete.

P24174-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQSKLYPVV MAGGSGSRLW PLSRVLYPKQ FLCLKGDLTM LQTTICRLNG
60 70 80 90 100
VECESPVVIC NEQHRFIVAE QLRQLNKLTE NIILEPAGRN TAPAIALAAL
110 120 130 140 150
AAKRHSPESD PLMLVLAADH VIADEDAFRA AVRNAMPYAE AGKLVTFGIV
160 170 180 190 200
PDLPETGYGY IRRGEVSAGE QDMVAFEVAQ FVEKPNLETA QAYVASGEYY
210 220 230 240 250
WNSGMFLFRA GRYLEELKKY RPDILDACEK AMSAVDPDLN FIRVDEEAFL
260 270 280 290 300
ACPEESVDYA VMERTADAVV VPMDAGWSDV GSWSSLWEIS AHTAEGNVCH
310 320 330 340 350
GDVINHKTEN SYVYAESGLV TTVGVKDLVV VQTKDAVLIA DRNAVQDVKK
360 370 380 390 400
VVEQIKADGR HEHRVHREVY RPWGKYDSID AGDRYQVKRI TVKPGEGLSV
410 420 430 440 450
QMHHHRAEHW VVVAGTAKVT IDGDIKLLGE NESIYIPLGA THCLENPGKI
460 470
PLDLIEVRSG SYLEEDDVVR FADRYGRV
Length:478
Mass (Da):53,016
Last modified:July 19, 2003 - v3
Checksum:i675C0EEA050B4186
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti325 – 3251V → L (PubMed:7815923).Curated
Sequence conflicti325 – 3251V → L (PubMed:8759852).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38473 Genomic DNA. Translation: AAC77846.1.
U00096 Genomic DNA. Translation: AAC75110.1.
AP009048 Genomic DNA. Translation: BAA15905.1.
M77127 Genomic DNA. Translation: AAA02893.1.
PIRiH64970.
RefSeqiNP_416553.1. NC_000913.3.
WP_000079274.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75110; AAC75110; b2049.
BAA15905; BAA15905; BAA15905.
GeneIDi946580.
KEGGiecj:JW2034.
eco:b2049.
PATRICi32119431. VBIEscCol129921_2126.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38473 Genomic DNA. Translation: AAC77846.1.
U00096 Genomic DNA. Translation: AAC75110.1.
AP009048 Genomic DNA. Translation: BAA15905.1.
M77127 Genomic DNA. Translation: AAA02893.1.
PIRiH64970.
RefSeqiNP_416553.1. NC_000913.3.
WP_000079274.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP24174.
SMRiP24174. Positions 9-355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263312. 176 interactions.
IntActiP24174. 5 interactions.
STRINGi511145.b2049.

Proteomic databases

PaxDbiP24174.
PRIDEiP24174.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75110; AAC75110; b2049.
BAA15905; BAA15905; BAA15905.
GeneIDi946580.
KEGGiecj:JW2034.
eco:b2049.
PATRICi32119431. VBIEscCol129921_2126.

Organism-specific databases

EchoBASEiEB0159.
EcoGeneiEG10161. manC.

Phylogenomic databases

eggNOGiENOG4105CJT. Bacteria.
COG0662. LUCA.
COG0836. LUCA.
HOGENOMiHOG000153549.
InParanoidiP24174.
KOiK00971.
OMAiETQIGEY.
PhylomeDBiP24174.

Enzyme and pathway databases

UniPathwayiUPA00126; UER00930.
BioCyciEcoCyc:MANNPGUANYLTRANGDP-MONOMER.
ECOL316407:JW2034-MONOMER.
MetaCyc:MANNPGUANYLTRANGDP-MONOMER.

Miscellaneous databases

PROiP24174.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
3.90.550.10. 1 hit.
InterProiIPR006375. Man1P_GuaTrfase/Man6P_Isoase.
IPR001538. Man6P_isomerase-2_C.
IPR005835. NTP_transferase_dom.
IPR029044. Nucleotide-diphossugar_trans.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF01050. MannoseP_isomer. 1 hit.
PF00483. NTP_transferase. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
SSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR01479. GMP_PMI. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMANC_ECOLI
AccessioniPrimary (citable) accession number: P24174
Secondary accession number(s): P78084
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: July 19, 2003
Last modified: September 7, 2016
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.