Reviewed, UniProtKB/Swiss-Prot P24173 (RFAC_ECOLI)

Names and origin

Protein names

Recommended name:
    Lipopolysaccharide heptosyltransferase 1
    EC=2.-.-.-

Gene names

Name:	rfaC
Synonyms:	rfa-2, waaC, yibC
Ordered Locus Names:	b3621, JW3596

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Heptose transfer to the lipopolysaccharide core. It transfers the innnermost heptose to [4'-P](3-deoxy-D-manno-octulosonic acid)2-IVA.
Pathway	Bacterial outer membrane biogenesis; LPS core biosynthesis.
Sequence similarities	Belongs to the glycosyltransferase 9 family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring glycosyl groups Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Lipopolysaccharide heptosyltransferase 1		PRO_0000207260

Sequences

	Sequence		Length	Mass (Da)
	P24173-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 31463CB533F33D33	FASTA	319	35,544
	10 20 30 40 50 60 MRVLIVKTSS MGDVLHTLPA LTDAQQAIPG IKFDWVVEEG FAQIPSWHAA VERVIPVAIR 70 80 90 100 110 120 RWRKAWFSAP IKAERKAFRE ALQAENYDAV IDAQGLVKSA ALVTRLAHGV KHGMDWQTAR 130 140 150 160 170 180 EPLASLFYNR KHHIAKQQHA VERTRELFAK SLGYSKPQTQ GDYAIAQHFL TNLPTDAGEY 190 200 210 220 230 240 AVFLHATTRD DKHWPEEHWR ELIGLLADSG IRIKLPWGAP HEEERAKRLA EGFAYVEVLP 250 260 270 280 290 300 KMSLEGVARV LAGAKFVVSV DTGLSHLTAA LDRPNITVYG PTDPGLIGGY GKNQMVCRAP 310 RENLINLNSQ AVLEKLSSL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of the Escherichia coli K-12 rfa-2 (rfaC) gene, a gene required for lipopolysaccharide inner core synthesis." Chen L., Coleman W.G. Jr. J. Bacteriol. 175:2534-2540(1993) [PubMed: 8478319] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-24. Strain: K12.
[2]	"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R. Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium." Klena J.D., Pradel E., Schnaitman C.A. J. Bacteriol. 174:4746-4752(1992) [PubMed: 1624462] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 264-319. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M95398 Genomic DNA. No translation available.
X62530 Genomic DNA. Translation: CAA44391.1.
U00039 Genomic DNA. Translation: AAB18598.1.
U00096 Genomic DNA. Translation: AAC76645.1.
AP009048 Genomic DNA. Translation: BAE77671.1.

PIR

A40619.

RefSeq

AP_004170.1.
NP_418078.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2H1H	X-ray	2.40	A/B	1-319	[»]

SMR

P24173. Positions 1-312.

ModBase

Search...

Protein family/group databases

CAZy

GT9. Glycosyltransferase Family 9.

Genome annotation databases

GeneID

948136.

GenomeReviews

Gene locus JW3596 in contig AP009048_GR.
Gene locus b3621 in contig U00096_GR.

KEGG

ecj:JW3596.
eco:b3621.

Organism-specific databases

EchoBASE

EB1175.

EcoGene

EG11189. rfaC.

CMR

Search...

Phylogenomic databases

HOGENOM

P24173.

OMA

P24173. VATRRWR.

Enzyme and pathway databases

BioCyc

EcoCyc:EG11189-MON.
MetaCyc:EG11189-MON.

Family and domain databases

InterPro

IPR002201. Glyco_trans_9.
IPR011908. Heptsyl_trn_I.
[Graphical view]

Pfam

PF01075. Glyco_transf_9. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02193. heptsyl_trn_I. 1 hit.

ProtoNet

Search...

Entry information

Entry name

RFAC_ECOLI

Accession

Primary (citable) accession number: P24173
Secondary accession number(s): P27376, Q2M7T5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)