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Protein

Lipopolysaccharide heptosyltransferase 1

Gene

rfaC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Heptose transfer to the lipopolysaccharide core. It transfers the innermost heptose to [4'-P](3-deoxy-D-manno-octulosonic acid)2-IVA.

Pathwayi: LPS core biosynthesis

This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.
View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.

GO - Molecular functioni

GO - Biological processi

  • lipopolysaccharide core region biosynthetic process Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG11189-MONOMER.
ECOL316407:JW3596-MONOMER.
MetaCyc:EG11189-MONOMER.
BRENDAi2.4.99.B6. 2026.
UniPathwayiUPA00958.

Protein family/group databases

CAZyiGT9. Glycosyltransferase Family 9.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipopolysaccharide heptosyltransferase 1 (EC:2.-.-.-)
Gene namesi
Name:rfaC
Synonyms:rfa-2, waaC, yibC
Ordered Locus Names:b3621, JW3596
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11189. rfaC.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002072601 – 319Lipopolysaccharide heptosyltransferase 1Add BLAST319

Proteomic databases

PaxDbiP24173.
PRIDEiP24173.

Interactioni

Protein-protein interaction databases

BioGridi4263304. 489 interactors.
IntActiP24173. 17 interactors.
STRINGi511145.b3621.

Structurei

Secondary structure

1319
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Helixi11 – 15Combined sources5
Helixi18 – 27Combined sources10
Beta strandi32 – 38Combined sources7
Helixi39 – 41Combined sources3
Helixi43 – 46Combined sources4
Beta strandi51 – 57Combined sources7
Helixi59 – 63Combined sources5
Turni64 – 67Combined sources4
Helixi69 – 83Combined sources15
Beta strandi84 – 86Combined sources3
Beta strandi88 – 92Combined sources5
Helixi97 – 102Combined sources6
Helixi104 – 106Combined sources3
Beta strandi107 – 109Combined sources3
Beta strandi111 – 114Combined sources4
Turni116 – 118Combined sources3
Beta strandi119 – 121Combined sources3
Helixi122 – 127Combined sources6
Beta strandi129 – 132Combined sources4
Beta strandi136 – 138Combined sources3
Helixi140 – 152Combined sources13
Helixi167 – 170Combined sources4
Turni175 – 178Combined sources4
Beta strandi179 – 184Combined sources6
Helixi190 – 192Combined sources3
Helixi196 – 206Combined sources11
Helixi207 – 209Combined sources3
Beta strandi212 – 215Combined sources4
Helixi220 – 230Combined sources11
Beta strandi236 – 238Combined sources3
Helixi244 – 252Combined sources9
Beta strandi257 – 262Combined sources6
Helixi263 – 270Combined sources8
Beta strandi275 – 282Combined sources8
Helixi284 – 287Combined sources4
Beta strandi294 – 298Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H1HX-ray2.40A/B1-300[»]
ProteinModelPortaliP24173.
SMRiP24173.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24173.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 9 family.Curated

Phylogenomic databases

eggNOGiENOG4105D16. Bacteria.
COG0859. LUCA.
HOGENOMiHOG000257134.
InParanoidiP24173.
KOiK02841.
OMAiEIPAWHP.
PhylomeDBiP24173.

Family and domain databases

InterProiIPR002201. Glyco_trans_9.
IPR011908. LipoPS_heptosylTferase-I.
[Graphical view]
PfamiPF01075. Glyco_transf_9. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02193. heptsyl_trn_I. 1 hit.

Sequencei

Sequence statusi: Complete.

P24173-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLIVKTSS MGDVLHTLPA LTDAQQAIPG IKFDWVVEEG FAQIPSWHAA
60 70 80 90 100
VERVIPVAIR RWRKAWFSAP IKAERKAFRE ALQAENYDAV IDAQGLVKSA
110 120 130 140 150
ALVTRLAHGV KHGMDWQTAR EPLASLFYNR KHHIAKQQHA VERTRELFAK
160 170 180 190 200
SLGYSKPQTQ GDYAIAQHFL TNLPTDAGEY AVFLHATTRD DKHWPEEHWR
210 220 230 240 250
ELIGLLADSG IRIKLPWGAP HEEERAKRLA EGFAYVEVLP KMSLEGVARV
260 270 280 290 300
LAGAKFVVSV DTGLSHLTAA LDRPNITVYG PTDPGLIGGY GKNQMVCRAP
310
RENLINLNSQ AVLEKLSSL
Length:319
Mass (Da):35,544
Last modified:March 1, 1992 - v1
Checksum:i31463CB533F33D33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95398 Genomic DNA. No translation available.
X62530 Genomic DNA. Translation: CAA44391.1.
U00039 Genomic DNA. Translation: AAB18598.1.
U00096 Genomic DNA. Translation: AAC76645.1.
AP009048 Genomic DNA. Translation: BAE77671.1.
PIRiA40619.
RefSeqiNP_418078.1. NC_000913.3.
WP_001264584.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76645; AAC76645; b3621.
BAE77671; BAE77671; BAE77671.
GeneIDi948136.
KEGGiecj:JW3596.
eco:b3621.
PATRICi32122729. VBIEscCol129921_3741.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95398 Genomic DNA. No translation available.
X62530 Genomic DNA. Translation: CAA44391.1.
U00039 Genomic DNA. Translation: AAB18598.1.
U00096 Genomic DNA. Translation: AAC76645.1.
AP009048 Genomic DNA. Translation: BAE77671.1.
PIRiA40619.
RefSeqiNP_418078.1. NC_000913.3.
WP_001264584.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2H1HX-ray2.40A/B1-300[»]
ProteinModelPortaliP24173.
SMRiP24173.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263304. 489 interactors.
IntActiP24173. 17 interactors.
STRINGi511145.b3621.

Protein family/group databases

CAZyiGT9. Glycosyltransferase Family 9.

Proteomic databases

PaxDbiP24173.
PRIDEiP24173.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76645; AAC76645; b3621.
BAE77671; BAE77671; BAE77671.
GeneIDi948136.
KEGGiecj:JW3596.
eco:b3621.
PATRICi32122729. VBIEscCol129921_3741.

Organism-specific databases

EchoBASEiEB1175.
EcoGeneiEG11189. rfaC.

Phylogenomic databases

eggNOGiENOG4105D16. Bacteria.
COG0859. LUCA.
HOGENOMiHOG000257134.
InParanoidiP24173.
KOiK02841.
OMAiEIPAWHP.
PhylomeDBiP24173.

Enzyme and pathway databases

UniPathwayiUPA00958.
BioCyciEcoCyc:EG11189-MONOMER.
ECOL316407:JW3596-MONOMER.
MetaCyc:EG11189-MONOMER.
BRENDAi2.4.99.B6. 2026.

Miscellaneous databases

EvolutionaryTraceiP24173.
PROiP24173.

Family and domain databases

InterProiIPR002201. Glyco_trans_9.
IPR011908. LipoPS_heptosylTferase-I.
[Graphical view]
PfamiPF01075. Glyco_transf_9. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02193. heptsyl_trn_I. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRFAC_ECOLI
AccessioniPrimary (citable) accession number: P24173
Secondary accession number(s): P27376, Q2M7T5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.