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P24173 (RFAC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipopolysaccharide heptosyltransferase 1
EC=2.-.-.-
Gene names
Name:rfaC
Synonyms:rfa-2, waaC, yibC
Ordered Locus Names:b3621, JW3596
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Heptose transfer to the lipopolysaccharide core. It transfers the innermost heptose to [4'-P](3-deoxy-D-manno-octulosonic acid)2-IVA.

Pathway

Bacterial outer membrane biogenesis; LPS core biosynthesis.

Sequence similarities

Belongs to the glycosyltransferase 9 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 319319Lipopolysaccharide heptosyltransferase 1
PRO_0000207260

Secondary structure

1 ........................................................ 319
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P24173 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 31463CB533F33D33

FASTA31935,544
        10         20         30         40         50         60 
MRVLIVKTSS MGDVLHTLPA LTDAQQAIPG IKFDWVVEEG FAQIPSWHAA VERVIPVAIR 

        70         80         90        100        110        120 
RWRKAWFSAP IKAERKAFRE ALQAENYDAV IDAQGLVKSA ALVTRLAHGV KHGMDWQTAR 

       130        140        150        160        170        180 
EPLASLFYNR KHHIAKQQHA VERTRELFAK SLGYSKPQTQ GDYAIAQHFL TNLPTDAGEY 

       190        200        210        220        230        240 
AVFLHATTRD DKHWPEEHWR ELIGLLADSG IRIKLPWGAP HEEERAKRLA EGFAYVEVLP 

       250        260        270        280        290        300 
KMSLEGVARV LAGAKFVVSV DTGLSHLTAA LDRPNITVYG PTDPGLIGGY GKNQMVCRAP 

       310 
RENLINLNSQ AVLEKLSSL

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of the Escherichia coli K-12 rfa-2 (rfaC) gene, a gene required for lipopolysaccharide inner core synthesis."
Chen L., Coleman W.G. Jr.
J. Bacteriol. 175:2534-2540(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-24.
Strain: K12.
[2]"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
Nucleic Acids Res. 22:2576-2586(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium."
Klena J.D., Pradel E., Schnaitman C.A.
J. Bacteriol. 174:4746-4752(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 264-319.
Strain: K12.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M95398 Genomic DNA. No translation available.
X62530 Genomic DNA. Translation: CAA44391.1.
U00039 Genomic DNA. Translation: AAB18598.1.
U00096 Genomic DNA. Translation: AAC76645.1.
AP009048 Genomic DNA. Translation: BAE77671.1.
PIRA40619.
RefSeqNP_418078.1. NC_000913.2.
YP_491812.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2H1HX-ray2.40A/B1-319[»]
ProteinModelPortalP24173.
SMRP24173. Positions 1-312.
ModBaseSearch...

Protein-protein interaction databases

IntActP24173. 17 interactions.
STRING511145.b3621.

Protein family/group databases

CAZyGT9. Glycosyltransferase Family 9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76645; AAC76645; b3621.
BAE77671; BAE77671; BAE77671.
GeneID12934310.
948136.
KEGGecj:Y75_p3553.
eco:b3621.
PATRIC32122729. VBIEscCol129921_3741.

Organism-specific databases

EchoBASEEB1175.
EcoGeneEG11189. rfaC.

Phylogenomic databases

eggNOGCOG0859.
HOGENOMHOG000257134.
KOK02841.
OMAWHPAVDR.
ProtClustDBPRK10964.

Enzyme and pathway databases

BioCycEcoCyc:EG11189-MONOMER.
ECOL316407:JW3596-MONOMER.
MetaCyc:EG11189-MONOMER.
UniPathwayUPA00958.

Gene expression databases

GenevestigatorP24173.

Family and domain databases

InterProIPR002201. Glyco_trans_9.
IPR011908. LipoPS_heptosylTferase-I.
[Graphical view]
PfamPF01075. Glyco_transf_9. 1 hit.
[Graphical view]
TIGRFAMsTIGR02193. heptsyl_trn_I. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP24173.

Entry information

Entry nameRFAC_ECOLI
AccessionPrimary (citable) accession number: P24173
Secondary accession number(s): P27376, Q2M7T5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: May 1, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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