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Reviewed, UniProtKB/Swiss-Prot P24173 (RFAC_ECOLI)

Last modified June 16, 2009. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Lipopolysaccharide heptosyltransferase 1
    EC=2.-.-.-
Gene names
Name: rfaC
Synonyms: rfa-2, waaC, yibC
Ordered Locus Names: b3621, JW3596
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Heptose transfer to the lipopolysaccharide core. It transfers the innnermost heptose to [4'-P](3-deoxy-D-manno-octulosonic acid)2-IVA.

Pathway

Bacterial outer membrane biogenesis; LPS core biosynthesis.

Sequence similarities

Belongs to the glycosyltransferase 9 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 319319Lipopolysaccharide heptosyltransferase 1
PRO_0000207260

Sequences

Sequence LengthMass (Da)Tools
P24173-1 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 31463CB533F33D33

FASTA31935,544
        10         20         30         40         50         60 
MRVLIVKTSS MGDVLHTLPA LTDAQQAIPG IKFDWVVEEG FAQIPSWHAA VERVIPVAIR 

        70         80         90        100        110        120 
RWRKAWFSAP IKAERKAFRE ALQAENYDAV IDAQGLVKSA ALVTRLAHGV KHGMDWQTAR 

       130        140        150        160        170        180 
EPLASLFYNR KHHIAKQQHA VERTRELFAK SLGYSKPQTQ GDYAIAQHFL TNLPTDAGEY 

       190        200        210        220        230        240 
AVFLHATTRD DKHWPEEHWR ELIGLLADSG IRIKLPWGAP HEEERAKRLA EGFAYVEVLP 

       250        260        270        280        290        300 
KMSLEGVARV LAGAKFVVSV DTGLSHLTAA LDRPNITVYG PTDPGLIGGY GKNQMVCRAP 

       310 
RENLINLNSQ AVLEKLSSL 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of the Escherichia coli K-12 rfa-2 (rfaC) gene, a gene required for lipopolysaccharide inner core synthesis."
Chen L., Coleman W.G. Jr.
J. Bacteriol. 175:2534-2540(1993) [PubMed: 8478319] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-24.
Strain: K12.
[2]"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes."
Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.
Nucleic Acids Res. 22:2576-2586(1994) [PubMed: 8041620] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium."
Klena J.D., Pradel E., Schnaitman C.A.
J. Bacteriol. 174:4746-4752(1992) [PubMed: 1624462] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 264-319.
Strain: K12.
+Additional computationally mapped references.

Cross-references

Sequence databases

M95398 Genomic DNA. No translation available.
X62530 Genomic DNA. Translation: CAA44391.1.
U00039 Genomic DNA. Translation: AAB18598.1.
U00096 Genomic DNA. Translation: AAC76645.1.
AP009048 Genomic DNA. Translation: BAE77671.1.
PIRA40619.
RefSeqAP_004170.1.
NP_418078.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2H1HX-ray2.40A/B1-319[»]
SMRP24173. Positions 1-312.
ModBaseSearch...

Protein family/group databases

CAZyGT9. Glycosyltransferase Family 9.

Genome annotation databases

GeneID948136.
GenomeReviewsGene locus JW3596 in contig AP009048_GR.
Gene locus b3621 in contig U00096_GR.
KEGGecj:JW3596.
eco:b3621.

Organism-specific databases

EchoBASEEB1175.
EcoGeneEG11189. rfaC.
CMRSearch...

Phylogenomic databases

HOGENOMP24173.
OMAP24173. VATRRWR.

Enzyme and pathway databases

BioCycEcoCyc:EG11189-MON.
MetaCyc:EG11189-MON.

Family and domain databases

InterProIPR002201. Glyco_trans_9.
IPR011908. Heptsyl_trn_I.
[Graphical view]
PfamPF01075. Glyco_transf_9. 1 hit.
[Graphical view]
TIGRFAMsTIGR02193. heptsyl_trn_I. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRFAC_ECOLI
AccessionPrimary (citable) accession number: P24173
Secondary accession number(s): P27376, Q2M7T5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents