P24161 (CD3Z_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
T-cell surface glycoprotein CD3 zeta chain

Alternative name(s):
T-cell receptor T3 zeta chain
CD_antigen=CD247

Gene names

Name:	Cd247
Synonyms:	Cd3z, Tcrz

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	164 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
Subunit structure	Interacts with DOCK2 and TRAT1. Interacts with SHB. Interacts with ZAP70. Interacts (tyrosine phosphorylated) with SHC1 (via SH2 domain). Interacts with PTPRC By similarity. The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3-zeta forms either homodimers or heterodimers with CD3-eta. Interacts with SLA and SLA2.
Subcellular location	Membrane; Single-pass type I membrane protein.
Domain	The ITAM domains mediate interaction with SHB By similarity.
Post-translational modification	Phosphorylated on Tyr residues after T-cell receptor triggering.
Sequence similarities	Belongs to the CD3Z/FCER1G family. Contains 3 ITAM domains.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	T cell receptor signaling pathway Inferred from direct assay. Source: MGI
Cellular component	alpha-beta T cell receptor complex Inferred from direct assay. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: MGI transmembrane signaling receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform CD-3-zeta (identifier: P24161-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform CD-3-eta (identifier: P29020-1)

The sequence of this isoform can be found in the external entry P29020.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21
	Chain	22 – 164	143	T-cell surface glycoprotein CD3 zeta chain		PRO_0000016494
Regions
	Topological domain	22 – 30	9	Extracellular Potential
	Transmembrane	31 – 51	21	Helical; Potential
	Topological domain	52 – 164	113	Cytoplasmic Potential
	Domain	61 – 89	29	ITAM 1
	Domain	100 – 128	29	ITAM 2
	Domain	131 – 159	29	ITAM 3
Amino acid modifications
	Modified residue	22	1	Blocked amino end (Gln)
	Modified residue	58	1	Phosphoserine By similarity
	Modified residue	72	1	Phosphotyrosine By similarity
	Modified residue	153	1	Phosphotyrosine By similarity
	Disulfide bond	32		Interchain Potential
Experimental info
	Sequence conflict	153	1	Y → C in BAB30997. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform CD-3-zeta [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 1B8022035A312831	FASTA	164	18,637
	10 20 30 40 50 60 MKWKVSVLAC ILHVRFPGAE AQSFGLLDPK LCYLLDGILF IYGVIITALY LRAKFSRSAE 70 80 90 100 110 120 TAANLQDPNQ LYNELNLGRR EEYDVLEKKR ARDPEMGGKQ QRRRNPQEGV YNALQKDKMA 130 140 150 160 EAYSEIGTKG ERRRGKGHDG LYQGLSTATK DTYDALHMQT LAPR
	Isoform CD-3-eta [UniParc]. See P29020.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the zeta chain of the T cell antigen receptor." Weissman A.M., Baniyash M., Hou D., Samelson L.E., Burgess W.H., Klausner R.D. Science 239:1018-1021(1988) [PubMed: 3278377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]	"The isolation and characterization of the murine T cell antigen receptor zeta chain gene." Baniyash M., Hsu V.W., Seldin M.F., Klausner R.D. J. Biol. Chem. 264:13252-13257(1989) [PubMed: 2787796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Thymus.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Hematopoietic.
[5]	"Molecular cloning of the CD3 eta subunit identifies a CD3 zeta-related product in thymus-derived cells." Jin Y.J., Clayton L.K., Howard F.D., Koyasu S., Sieh M., Steinbrich R., Tarr G.E., Reinherz E.L. Proc. Natl. Acad. Sci. U.S.A. 87:3319-3323(1990) [PubMed: 2139725] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, BLOCKAGE OF N-TERMINUS.
[6]	"CD3 zeta and eta chains are produced by alternative splicing from a common gene." Ohno H., Saito T. Int. Immunol. 2:1117-1119(1990) [PubMed: 2150596] [Abstract] Cited for: ALTERNATIVE SPLICING.
[7]	Erratum Ohno H., Saito T. Int. Immunol. 4:1339-1339(1992)
[8]	"Src-like adaptor protein (SLAP) is a negative regulator of T cell receptor signaling." Sosinowski T., Pandey A., Dixit V.M., Weiss A. J. Exp. Med. 191:463-474(2000) [PubMed: 10662792] [Abstract] Cited for: INTERACTION WITH SLA.
[9]	"A novel Src homology 2 domain-containing molecule, Src-like adapter protein-2 (SLAP-2), which negatively regulates T cell receptor signaling." Pandey A., Ibarrola N., Kratchmarova I., Fernandez M.M., Constantinescu S.N., Ohara O., Sawasdikosol S., Lodish H.F., Mann M. J. Biol. Chem. 277:19131-19138(2002) [PubMed: 11891219] [Abstract] Cited for: INTERACTION WITH SLA2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M19729 Genomic DNA. Translation: AAA40171.1. J04967 mRNA. Translation: AAA50301.1. AK017904 mRNA. Translation: BAB30997.1. BC052824 mRNA. Translation: AAH52824.1.
IPI	IPI00133467.
PIR	A40104.
RefSeq	NP_001106862.1. NM_001113391.2.
UniGene	Mm.217308. Mm.475205.
3D structure databases
ProteinModelPortal	P24161.
SMR	P24161. Positions 28-60.
ModBase	Search...
Protein-protein interaction databases
STRING	P24161.
PTM databases
PhosphoSite	P24161.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000005907; ENSMUSP00000005907; ENSMUSG00000005763.
GeneID	12503.
KEGG	mmu:12503.
UCSC	uc007djn.2. mouse.
Organism-specific databases
CTD	919.
MGI	MGI:88334. Cd247.
Phylogenomic databases
GeneTree	ENSGT00390000018208.
HOVERGEN	HBG005280.
OMA	PRLCYLL.
Gene expression databases
ArrayExpress	P24161.
Bgee	P24161.
CleanEx	MM_CD247.
Genevestigator	P24161.
GermOnline	ENSMUSG00000005763. Mus musculus.
Family and domain databases
InterPro	IPR021663. CR3_zeta/IgE_Fc_rcpt_gamma. IPR003110. Phos_immunorcpt_sig_ITAM. IPR024128. T-cell_CD3_zeta/eta. [Graphical view]
KO	K06453.
PANTHER	PTHR10035. PTHR10035. 1 hit.
Pfam	PF02189. ITAM. 3 hits. PF11628. TCR_zetazeta. 1 hit. [Graphical view]
SMART	SM00077. ITAM. 3 hits. [Graphical view]
PROSITE	PS51055. ITAM_1. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	281458.
SOURCE	Search...

Entry information

Entry name

CD3Z_MOUSE

Accession

Primary (citable) accession number: P24161
Secondary accession number(s): Q9D3G3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	January 25, 2012
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program