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P24161 (CD3Z_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
T-cell surface glycoprotein CD3 zeta chain
Alternative name(s):
T-cell receptor T3 zeta chain
CD_antigen=CD247
Gene names
Name:Cd247
Synonyms:Cd3z, Tcrz
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length164 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.

Subunit structure

Interacts with DOCK2 and TRAT1. Interacts with SHB. Interacts with ZAP70. Interacts (tyrosine phosphorylated) with SHC1 (via SH2 domain). Interacts with PTPRC By similarity. The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3-zeta forms either homodimers or heterodimers with CD3-eta. Interacts with SLA and SLA2. Ref.8 Ref.9

Subcellular location

Membrane; Single-pass type I membrane protein.

Domain

The ITAM domains mediate interaction with SHB By similarity.

Post-translational modification

Phosphorylated on Tyr residues after T-cell receptor triggering.

Sequence similarities

Belongs to the CD3Z/FCER1G family.

Contains 3 ITAM domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Ptpn4Q9WU223EBI-7803400,EBI-7249866

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform CD-3-zeta (identifier: P24161-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform CD-3-eta (identifier: P29020-1)

The sequence of this isoform can be found in the external entry P29020.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121
Chain22 – 164143T-cell surface glycoprotein CD3 zeta chain
PRO_0000016494

Regions

Topological domain22 – 309Extracellular Potential
Transmembrane31 – 5121Helical; Potential
Topological domain52 – 164113Cytoplasmic Potential
Domain61 – 8929ITAM 1
Domain100 – 12829ITAM 2
Domain131 – 15929ITAM 3

Amino acid modifications

Modified residue221Blocked amino end (Gln)
Modified residue581Phosphoserine By similarity
Modified residue721Phosphotyrosine By similarity
Modified residue831Phosphotyrosine By similarity
Modified residue1111Phosphotyrosine By similarity
Modified residue1231Phosphotyrosine By similarity
Modified residue1421Phosphotyrosine By similarity
Modified residue1531Phosphotyrosine By similarity
Disulfide bond32Interchain Potential

Experimental info

Sequence conflict1531Y → C in BAB30997. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform CD-3-zeta [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 1B8022035A312831

FASTA16418,637
        10         20         30         40         50         60 
MKWKVSVLAC ILHVRFPGAE AQSFGLLDPK LCYLLDGILF IYGVIITALY LRAKFSRSAE 

        70         80         90        100        110        120 
TAANLQDPNQ LYNELNLGRR EEYDVLEKKR ARDPEMGGKQ QRRRNPQEGV YNALQKDKMA 

       130        140        150        160 
EAYSEIGTKG ERRRGKGHDG LYQGLSTATK DTYDALHMQT LAPR 

« Hide

Isoform CD-3-eta [UniParc].

See P29020.

References

« Hide 'large scale' references
[1]"Molecular cloning of the zeta chain of the T cell antigen receptor."
Weissman A.M., Baniyash M., Hou D., Samelson L.E., Burgess W.H., Klausner R.D.
Science 239:1018-1021(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
[2]"The isolation and characterization of the murine T cell antigen receptor zeta chain gene."
Baniyash M., Hsu V.W., Seldin M.F., Klausner R.D.
J. Biol. Chem. 264:13252-13257(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Liver.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Thymus.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Hematopoietic.
[5]"Molecular cloning of the CD3 eta subunit identifies a CD3 zeta-related product in thymus-derived cells."
Jin Y.J., Clayton L.K., Howard F.D., Koyasu S., Sieh M., Steinbrich R., Tarr G.E., Reinherz E.L.
Proc. Natl. Acad. Sci. U.S.A. 87:3319-3323(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE, BLOCKAGE OF N-TERMINUS.
[6]"CD3 zeta and eta chains are produced by alternative splicing from a common gene."
Ohno H., Saito T.
Int. Immunol. 2:1117-1119(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: ALTERNATIVE SPLICING.
[7]Erratum
Ohno H., Saito T.
Int. Immunol. 4:1339-1339(1992)
[8]"Src-like adaptor protein (SLAP) is a negative regulator of T cell receptor signaling."
Sosinowski T., Pandey A., Dixit V.M., Weiss A.
J. Exp. Med. 191:463-474(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SLA.
[9]"A novel Src homology 2 domain-containing molecule, Src-like adapter protein-2 (SLAP-2), which negatively regulates T cell receptor signaling."
Pandey A., Ibarrola N., Kratchmarova I., Fernandez M.M., Constantinescu S.N., Ohara O., Sawasdikosol S., Lodish H.F., Mann M.
J. Biol. Chem. 277:19131-19138(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SLA2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M19729 Genomic DNA. Translation: AAA40171.1.
J04967 mRNA. Translation: AAA50301.1.
AK017904 mRNA. Translation: BAB30997.1.
BC052824 mRNA. Translation: AAH52824.1.
PIRA40104.
RefSeqNP_001106862.1. NM_001113391.2.
XP_006496696.1. XM_006496633.1.
UniGeneMm.217308.
Mm.245261.
Mm.475205.

3D structure databases

ProteinModelPortalP24161.
SMRP24161. Positions 28-60.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198598. 4 interactions.
IntActP24161. 5 interactions.
MINTMINT-7895650.

PTM databases

PhosphoSiteP24161.

Protocols and materials databases

DNASU12503.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000005907; ENSMUSP00000005907; ENSMUSG00000005763. [P24161-1]
GeneID12503.
KEGGmmu:12503.
UCSCuc007djn.2. mouse. [P24161-1]

Organism-specific databases

CTD919.
MGIMGI:88334. Cd247.

Phylogenomic databases

GeneTreeENSGT00390000018208.
HOGENOMHOG000234398.
HOVERGENHBG005280.
KOK06453.
OMALHMQTLP.

Gene expression databases

ArrayExpressP24161.
BgeeP24161.
CleanExMM_CD247.
GenevestigatorP24161.

Family and domain databases

InterProIPR021663. CR3_zeta/IgE_Fc_rcpt_gamma.
IPR003110. Phos_immunorcpt_sig_ITAM.
IPR024128. T-cell_CD3_zeta/eta.
[Graphical view]
PANTHERPTHR10035. PTHR10035. 1 hit.
PfamPF02189. ITAM. 3 hits.
PF11628. TCR_zetazeta. 1 hit.
[Graphical view]
SMARTSM00077. ITAM. 3 hits.
[Graphical view]
PROSITEPS51055. ITAM_1. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCD247. mouse.
NextBio281458.
PROP24161.
SOURCESearch...

Entry information

Entry nameCD3Z_MOUSE
AccessionPrimary (citable) accession number: P24161
Secondary accession number(s): Q9D3G3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: April 16, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot