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Protein

T-cell surface glycoprotein CD3 zeta chain

Gene

Cd247

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.

GO - Molecular functioni

GO - Biological processi

  • T cell receptor signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-MMU-202424. Downstream TCR signaling.
R-MMU-202427. Phosphorylation of CD3 and TCR zeta chains.
R-MMU-202430. Translocation of ZAP-70 to Immunological synapse.
R-MMU-202433. Generation of second messenger molecules.
R-MMU-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-MMU-2029485. Role of phospholipids in phagocytosis.
R-MMU-389948. PD-1 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
T-cell surface glycoprotein CD3 zeta chain
Alternative name(s):
T-cell receptor T3 zeta chain
CD_antigen: CD247
Gene namesi
Name:Cd247
Synonyms:Cd3z, Tcrz
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:88334. Cd247.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 30ExtracellularSequence analysis9
Transmembranei31 – 51HelicalSequence analysisAdd BLAST21
Topological domaini52 – 164CytoplasmicSequence analysisAdd BLAST113

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000001649422 – 164T-cell surface glycoprotein CD3 zeta chainAdd BLAST143

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei22Blocked amino end (Gln)1
Disulfide bondi32InterchainSequence analysis
Modified residuei58PhosphoserineBy similarity1
Modified residuei72PhosphotyrosinePROSITE-ProRule annotationBy similarity1
Modified residuei83PhosphotyrosinePROSITE-ProRule annotationBy similarity1
Modified residuei111PhosphotyrosinePROSITE-ProRule annotationBy similarity1
Modified residuei123PhosphotyrosinePROSITE-ProRule annotationBy similarity1
Modified residuei142PhosphotyrosinePROSITE-ProRule annotationBy similarity1
Modified residuei153PhosphotyrosinePROSITE-ProRule annotationBy similarity1

Post-translational modificationi

Phosphorylated on Tyr residues after T-cell receptor triggering.

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

EPDiP24161.
PaxDbiP24161.
P29020.
PRIDEiP24161.

PTM databases

iPTMnetiP24161.
PhosphoSitePlusiP24161.

Expressioni

Gene expression databases

BgeeiENSMUSG00000005763.
CleanExiMM_CD247.
ExpressionAtlasiP24161. baseline and differential.
GenevisibleiP24161. MM.

Interactioni

Subunit structurei

Interacts with DOCK2 and TRAT1. Interacts with SHB. Interacts with ZAP70. Interacts (tyrosine phosphorylated) with SHC1 (via SH2 domain). Interacts with PTPRC (By similarity). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3-zeta forms either homodimers or heterodimers with CD3-eta. Interacts with SLA and SLA2.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Ptpn4Q9WU223EBI-7803400,EBI-7249866

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198598. 5 interactors.
IntActiP24161. 6 interactors.
MINTiMINT-7895650.
STRINGi10090.ENSMUSP00000027849.

Structurei

3D structure databases

ProteinModelPortaliP24161.
SMRiP24161.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini61 – 89ITAM 1PROSITE-ProRule annotationAdd BLAST29
Domaini100 – 128ITAM 2PROSITE-ProRule annotationAdd BLAST29
Domaini131 – 159ITAM 3PROSITE-ProRule annotationAdd BLAST29

Domaini

The ITAM domains mediate interaction with SHB.By similarity

Sequence similaritiesi

Belongs to the CD3Z/FCER1G family.Curated
Contains 3 ITAM domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J0ID. Eukaryota.
ENOG411241T. LUCA.
GeneTreeiENSGT00390000018208.
HOGENOMiHOG000234398.
HOVERGENiHBG005280.
InParanoidiP29020.
KOiK06453.
OMAiLHMQTLP.
OrthoDBiEOG091G0QJC.

Family and domain databases

InterProiIPR021663. CD3_zeta/IgE_Fc_rcpt_gamma.
IPR003110. Phos_immunorcpt_sig_ITAM.
IPR024128. T-cell_CD3_zeta.
[Graphical view]
PANTHERiPTHR10035. PTHR10035. 1 hit.
PfamiPF02189. ITAM. 3 hits.
PF11628. TCR_zetazeta. 1 hit.
[Graphical view]
SMARTiSM00077. ITAM. 3 hits.
[Graphical view]
PROSITEiPS51055. ITAM_1. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform CD-3-zeta (identifier: P24161-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKWKVSVLAC ILHVRFPGAE AQSFGLLDPK LCYLLDGILF IYGVIITALY
60 70 80 90 100
LRAKFSRSAE TAANLQDPNQ LYNELNLGRR EEYDVLEKKR ARDPEMGGKQ
110 120 130 140 150
QRRRNPQEGV YNALQKDKMA EAYSEIGTKG ERRRGKGHDG LYQGLSTATK
160
DTYDALHMQT LAPR
Length:164
Mass (Da):18,637
Last modified:March 1, 1992 - v1
Checksum:i1B8022035A312831
GO
Isoform CD-3-eta (identifier: P24161-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     144-164: GLSTATKDTYDALHMQTLAPR → DSHFQAVQFG...PWPPSSSSQL

Show »
Length:206
Mass (Da):23,339
Checksum:i829256A2CF44E444
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti153Y → C in BAB30997 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_058346144 – 164GLSTA…TLAPR → DSHFQAVQFGNRREREGSEL TRTLGLRARPKGESTQQSSQ SCASVFSIPTLWSPWPPSSS SQL in isoform CD-3-eta. Add BLAST21

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19729 Genomic DNA. Translation: AAA40171.1.
J04967 mRNA. Translation: AAA50301.1.
M33158 mRNA. Translation: AAA37398.1.
AK017904 mRNA. Translation: BAB30997.1.
BC052824 mRNA. Translation: AAH52824.1.
M76711 Genomic DNA. Translation: AAA40403.1.
CCDSiCCDS15443.1. [P24161-2]
CCDS48427.1. [P24161-1]
PIRiA35900.
A40104.
RefSeqiNP_001106862.1. NM_001113391.2. [P24161-1]
NP_112439.1. NM_031162.4. [P24161-2]
XP_011237047.1. XM_011238745.2. [P24161-1]
XP_017168774.1. XM_017313285.1. [P24161-1]
UniGeneiMm.217308.
Mm.245261.
Mm.475205.

Genome annotation databases

EnsembliENSMUST00000005907; ENSMUSP00000005907; ENSMUSG00000005763. [P24161-1]
ENSMUST00000027849; ENSMUSP00000027849; ENSMUSG00000005763. [P24161-2]
ENSMUST00000086002; ENSMUSP00000083165; ENSMUSG00000005763. [P24161-2]
ENSMUST00000187313; ENSMUSP00000140926; ENSMUSG00000005763. [P24161-2]
GeneIDi12503.
KEGGimmu:12503.
UCSCiuc007djn.2. mouse. [P24161-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19729 Genomic DNA. Translation: AAA40171.1.
J04967 mRNA. Translation: AAA50301.1.
M33158 mRNA. Translation: AAA37398.1.
AK017904 mRNA. Translation: BAB30997.1.
BC052824 mRNA. Translation: AAH52824.1.
M76711 Genomic DNA. Translation: AAA40403.1.
CCDSiCCDS15443.1. [P24161-2]
CCDS48427.1. [P24161-1]
PIRiA35900.
A40104.
RefSeqiNP_001106862.1. NM_001113391.2. [P24161-1]
NP_112439.1. NM_031162.4. [P24161-2]
XP_011237047.1. XM_011238745.2. [P24161-1]
XP_017168774.1. XM_017313285.1. [P24161-1]
UniGeneiMm.217308.
Mm.245261.
Mm.475205.

3D structure databases

ProteinModelPortaliP24161.
SMRiP24161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198598. 5 interactors.
IntActiP24161. 6 interactors.
MINTiMINT-7895650.
STRINGi10090.ENSMUSP00000027849.

PTM databases

iPTMnetiP24161.
PhosphoSitePlusiP24161.

Proteomic databases

EPDiP24161.
PaxDbiP24161.
P29020.
PRIDEiP24161.

Protocols and materials databases

DNASUi12503.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005907; ENSMUSP00000005907; ENSMUSG00000005763. [P24161-1]
ENSMUST00000027849; ENSMUSP00000027849; ENSMUSG00000005763. [P24161-2]
ENSMUST00000086002; ENSMUSP00000083165; ENSMUSG00000005763. [P24161-2]
ENSMUST00000187313; ENSMUSP00000140926; ENSMUSG00000005763. [P24161-2]
GeneIDi12503.
KEGGimmu:12503.
UCSCiuc007djn.2. mouse. [P24161-1]

Organism-specific databases

CTDi919.
MGIiMGI:88334. Cd247.

Phylogenomic databases

eggNOGiENOG410J0ID. Eukaryota.
ENOG411241T. LUCA.
GeneTreeiENSGT00390000018208.
HOGENOMiHOG000234398.
HOVERGENiHBG005280.
InParanoidiP29020.
KOiK06453.
OMAiLHMQTLP.
OrthoDBiEOG091G0QJC.

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-MMU-202424. Downstream TCR signaling.
R-MMU-202427. Phosphorylation of CD3 and TCR zeta chains.
R-MMU-202430. Translocation of ZAP-70 to Immunological synapse.
R-MMU-202433. Generation of second messenger molecules.
R-MMU-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-MMU-2029485. Role of phospholipids in phagocytosis.
R-MMU-389948. PD-1 signaling.

Miscellaneous databases

ChiTaRSiCd247. mouse.
PROiP24161.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005763.
CleanExiMM_CD247.
ExpressionAtlasiP24161. baseline and differential.
GenevisibleiP24161. MM.

Family and domain databases

InterProiIPR021663. CD3_zeta/IgE_Fc_rcpt_gamma.
IPR003110. Phos_immunorcpt_sig_ITAM.
IPR024128. T-cell_CD3_zeta.
[Graphical view]
PANTHERiPTHR10035. PTHR10035. 1 hit.
PfamiPF02189. ITAM. 3 hits.
PF11628. TCR_zetazeta. 1 hit.
[Graphical view]
SMARTiSM00077. ITAM. 3 hits.
[Graphical view]
PROSITEiPS51055. ITAM_1. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD3Z_MOUSE
AccessioniPrimary (citable) accession number: P24161
Secondary accession number(s): P29020, Q9D3G3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 30, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.