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P24161

- CD3Z_MOUSE

UniProt

P24161 - CD3Z_MOUSE

Protein

T-cell surface glycoprotein CD3 zeta chain

Gene

Cd247

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
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    Functioni

    Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. transmembrane signaling receptor activity Source: InterPro

    GO - Biological processi

    1. T cell receptor signaling pathway Source: MGI

    Keywords - Molecular functioni

    Receptor

    Enzyme and pathway databases

    ReactomeiREACT_196487. FCGR activation.
    REACT_198374. Regulation of actin dynamics for phagocytic cup formation.
    REACT_202937. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
    REACT_210240. Role of phospholipids in phagocytosis.
    REACT_215414. Translocation of ZAP-70 to Immunological synapse.
    REACT_216080. Phosphorylation of CD3 and TCR zeta chains.
    REACT_225145. Downstream TCR signaling.
    REACT_225768. Generation of second messenger molecules.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    T-cell surface glycoprotein CD3 zeta chain
    Alternative name(s):
    T-cell receptor T3 zeta chain
    CD_antigen: CD247
    Gene namesi
    Name:Cd247
    Synonyms:Cd3z, Tcrz
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:88334. Cd247.

    Subcellular locationi

    GO - Cellular componenti

    1. alpha-beta T cell receptor complex Source: MGI
    2. cytoplasm Source: MGI
    3. integral component of membrane Source: UniProtKB-KW
    4. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Add
    BLAST
    Chaini22 – 164143T-cell surface glycoprotein CD3 zeta chainPRO_0000016494Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei22 – 221Blocked amino end (Gln)
    Disulfide bondi32 – 32InterchainSequence Analysis
    Modified residuei58 – 581PhosphoserineBy similarity
    Modified residuei72 – 721PhosphotyrosinePROSITE-ProRule annotation
    Modified residuei83 – 831PhosphotyrosinePROSITE-ProRule annotation
    Modified residuei111 – 1111PhosphotyrosinePROSITE-ProRule annotation
    Modified residuei123 – 1231PhosphotyrosinePROSITE-ProRule annotation
    Modified residuei142 – 1421PhosphotyrosinePROSITE-ProRule annotation
    Modified residuei153 – 1531PhosphotyrosinePROSITE-ProRule annotation

    Post-translational modificationi

    Phosphorylated on Tyr residues after T-cell receptor triggering.

    Keywords - PTMi

    Disulfide bond, Phosphoprotein

    PTM databases

    PhosphoSiteiP24161.

    Expressioni

    Gene expression databases

    ArrayExpressiP24161.
    BgeeiP24161.
    CleanExiMM_CD247.
    GenevestigatoriP24161.

    Interactioni

    Subunit structurei

    Interacts with DOCK2 and TRAT1. Interacts with SHB. Interacts with ZAP70. Interacts (tyrosine phosphorylated) with SHC1 (via SH2 domain). Interacts with PTPRC By similarity. The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3-zeta forms either homodimers or heterodimers with CD3-eta. Interacts with SLA and SLA2.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Ptpn4Q9WU223EBI-7803400,EBI-7249866

    Protein-protein interaction databases

    BioGridi198598. 5 interactions.
    IntActiP24161. 5 interactions.
    MINTiMINT-7895650.

    Structurei

    3D structure databases

    ProteinModelPortaliP24161.
    SMRiP24161. Positions 28-60.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini22 – 309ExtracellularSequence Analysis
    Topological domaini52 – 164113CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei31 – 5121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini61 – 8929ITAM 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini100 – 12829ITAM 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini131 – 15929ITAM 3PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The ITAM domains mediate interaction with SHB.By similarity

    Sequence similaritiesi

    Belongs to the CD3Z/FCER1G family.Curated
    Contains 3 ITAM domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    GeneTreeiENSGT00390000018208.
    HOGENOMiHOG000234398.
    HOVERGENiHBG005280.
    KOiK06453.
    OMAiLHMQTLP.

    Family and domain databases

    InterProiIPR021663. CR3_zeta/IgE_Fc_rcpt_gamma.
    IPR003110. Phos_immunorcpt_sig_ITAM.
    IPR024128. T-cell_CD3_zeta/eta.
    [Graphical view]
    PANTHERiPTHR10035. PTHR10035. 1 hit.
    PfamiPF02189. ITAM. 3 hits.
    PF11628. TCR_zetazeta. 1 hit.
    [Graphical view]
    SMARTiSM00077. ITAM. 3 hits.
    [Graphical view]
    PROSITEiPS51055. ITAM_1. 3 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform CD-3-zeta (identifier: P24161-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKWKVSVLAC ILHVRFPGAE AQSFGLLDPK LCYLLDGILF IYGVIITALY    50
    LRAKFSRSAE TAANLQDPNQ LYNELNLGRR EEYDVLEKKR ARDPEMGGKQ 100
    QRRRNPQEGV YNALQKDKMA EAYSEIGTKG ERRRGKGHDG LYQGLSTATK 150
    DTYDALHMQT LAPR 164
    Length:164
    Mass (Da):18,637
    Last modified:March 1, 1992 - v1
    Checksum:i1B8022035A312831
    GO
    Isoform CD-3-eta (identifier: P29020-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform can be found in the external entry P29020.
    Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
    Length:206
    Mass (Da):23,339
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti153 – 1531Y → C in BAB30997. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M19729 Genomic DNA. Translation: AAA40171.1.
    J04967 mRNA. Translation: AAA50301.1.
    AK017904 mRNA. Translation: BAB30997.1.
    BC052824 mRNA. Translation: AAH52824.1.
    CCDSiCCDS48427.1. [P24161-1]
    PIRiA40104.
    RefSeqiNP_001106862.1. NM_001113391.2. [P24161-1]
    XP_006496696.1. XM_006496633.1. [P24161-1]
    UniGeneiMm.217308.
    Mm.245261.
    Mm.475205.

    Genome annotation databases

    EnsembliENSMUST00000005907; ENSMUSP00000005907; ENSMUSG00000005763. [P24161-1]
    GeneIDi12503.
    KEGGimmu:12503.
    UCSCiuc007djn.2. mouse. [P24161-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M19729 Genomic DNA. Translation: AAA40171.1 .
    J04967 mRNA. Translation: AAA50301.1 .
    AK017904 mRNA. Translation: BAB30997.1 .
    BC052824 mRNA. Translation: AAH52824.1 .
    CCDSi CCDS48427.1. [P24161-1 ]
    PIRi A40104.
    RefSeqi NP_001106862.1. NM_001113391.2. [P24161-1 ]
    XP_006496696.1. XM_006496633.1. [P24161-1 ]
    UniGenei Mm.217308.
    Mm.245261.
    Mm.475205.

    3D structure databases

    ProteinModelPortali P24161.
    SMRi P24161. Positions 28-60.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198598. 5 interactions.
    IntActi P24161. 5 interactions.
    MINTi MINT-7895650.

    PTM databases

    PhosphoSitei P24161.

    Protocols and materials databases

    DNASUi 12503.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000005907 ; ENSMUSP00000005907 ; ENSMUSG00000005763 . [P24161-1 ]
    GeneIDi 12503.
    KEGGi mmu:12503.
    UCSCi uc007djn.2. mouse. [P24161-1 ]

    Organism-specific databases

    CTDi 919.
    MGIi MGI:88334. Cd247.

    Phylogenomic databases

    GeneTreei ENSGT00390000018208.
    HOGENOMi HOG000234398.
    HOVERGENi HBG005280.
    KOi K06453.
    OMAi LHMQTLP.

    Enzyme and pathway databases

    Reactomei REACT_196487. FCGR activation.
    REACT_198374. Regulation of actin dynamics for phagocytic cup formation.
    REACT_202937. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
    REACT_210240. Role of phospholipids in phagocytosis.
    REACT_215414. Translocation of ZAP-70 to Immunological synapse.
    REACT_216080. Phosphorylation of CD3 and TCR zeta chains.
    REACT_225145. Downstream TCR signaling.
    REACT_225768. Generation of second messenger molecules.

    Miscellaneous databases

    ChiTaRSi CD247. mouse.
    NextBioi 281458.
    PROi P24161.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P24161.
    Bgeei P24161.
    CleanExi MM_CD247.
    Genevestigatori P24161.

    Family and domain databases

    InterProi IPR021663. CR3_zeta/IgE_Fc_rcpt_gamma.
    IPR003110. Phos_immunorcpt_sig_ITAM.
    IPR024128. T-cell_CD3_zeta/eta.
    [Graphical view ]
    PANTHERi PTHR10035. PTHR10035. 1 hit.
    Pfami PF02189. ITAM. 3 hits.
    PF11628. TCR_zetazeta. 1 hit.
    [Graphical view ]
    SMARTi SM00077. ITAM. 3 hits.
    [Graphical view ]
    PROSITEi PS51055. ITAM_1. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the zeta chain of the T cell antigen receptor."
      Weissman A.M., Baniyash M., Hou D., Samelson L.E., Burgess W.H., Klausner R.D.
      Science 239:1018-1021(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    2. "The isolation and characterization of the murine T cell antigen receptor zeta chain gene."
      Baniyash M., Hsu V.W., Seldin M.F., Klausner R.D.
      J. Biol. Chem. 264:13252-13257(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Thymus.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Hematopoietic.
    5. "Molecular cloning of the CD3 eta subunit identifies a CD3 zeta-related product in thymus-derived cells."
      Jin Y.J., Clayton L.K., Howard F.D., Koyasu S., Sieh M., Steinbrich R., Tarr G.E., Reinherz E.L.
      Proc. Natl. Acad. Sci. U.S.A. 87:3319-3323(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE, BLOCKAGE OF N-TERMINUS.
    6. "CD3 zeta and eta chains are produced by alternative splicing from a common gene."
      Ohno H., Saito T.
      Int. Immunol. 2:1117-1119(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: ALTERNATIVE SPLICING.
    7. Erratum
      Ohno H., Saito T.
      Int. Immunol. 4:1339-1339(1992)
    8. "Src-like adaptor protein (SLAP) is a negative regulator of T cell receptor signaling."
      Sosinowski T., Pandey A., Dixit V.M., Weiss A.
      J. Exp. Med. 191:463-474(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SLA.
    9. "A novel Src homology 2 domain-containing molecule, Src-like adapter protein-2 (SLAP-2), which negatively regulates T cell receptor signaling."
      Pandey A., Ibarrola N., Kratchmarova I., Fernandez M.M., Constantinescu S.N., Ohara O., Sawasdikosol S., Lodish H.F., Mann M.
      J. Biol. Chem. 277:19131-19138(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SLA2.

    Entry informationi

    Entry nameiCD3Z_MOUSE
    AccessioniPrimary (citable) accession number: P24161
    Secondary accession number(s): Q9D3G3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3