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Protein

Myeloblastin

Gene

PRTN3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters.

Catalytic activityi

Hydrolysis of proteins, including elastin, by preferential cleavage: -Ala-|-Xaa- > -Val-|-Xaa-.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei71Charge relay system1
Active sitei118Charge relay system1
Active sitei203Charge relay system1

GO - Molecular functioni

  • enzyme binding Source: UniProtKB
  • serine-type endopeptidase activity Source: GO_Central
  • serine-type peptidase activity Source: ProtInc

GO - Biological processi

  • blood coagulation Source: Reactome
  • collagen catabolic process Source: UniProtKB-KW
  • mature conventional dendritic cell differentiation Source: UniProtKB
  • negative regulation of phagocytosis Source: UniProtKB
  • positive regulation of cell proliferation Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Collagen degradation

Enzyme and pathway databases

BioCyciZFISH:HS10473-MONOMER.
BRENDAi3.4.21.76. 2681.
ReactomeiR-HSA-1222556. ROS, RNS production in response to bacteria.
R-HSA-140875. Common Pathway of Fibrin Clot Formation.
R-HSA-449147. Signaling by Interleukins.
R-HSA-6798695. Neutrophil degranulation.
R-HSA-6803157. Antimicrobial peptides.

Protein family/group databases

MEROPSiS01.134.

Names & Taxonomyi

Protein namesi
Recommended name:
Myeloblastin (EC:3.4.21.76)
Alternative name(s):
AGP7
C-ANCA antigen
Leukocyte proteinase 3
Short name:
PR-3
Short name:
PR3
Neutrophil proteinase 4
Short name:
NP-4
P29
Wegener autoantigen
Gene namesi
Name:PRTN3
Synonyms:MBN
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:9495. PRTN3.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi5657.
MalaCardsiPRTN3.
OpenTargetsiENSG00000196415.
ENSG00000277804.
Orphaneti900. Granulomatosis with polyangiitis.
PharmGKBiPA33842.

Chemistry databases

ChEMBLiCHEMBL3900.
GuidetoPHARMACOLOGYi2401.

Polymorphism and mutation databases

BioMutaiPRTN3.
DMDMi6174926.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Add BLAST25
PropeptideiPRO_000002770726 – 272
ChainiPRO_000002770828 – 248MyeloblastinAdd BLAST221
PropeptideiPRO_0000027709249 – 2568

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi56 ↔ 72
Glycosylationi129N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi152 ↔ 209
Glycosylationi174N-linked (GlcNAc...)1
Disulfide bondi182 ↔ 188
Disulfide bondi199 ↔ 224

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiP24158.
PaxDbiP24158.
PeptideAtlasiP24158.
PRIDEiP24158.

Expressioni

Gene expression databases

BgeeiENSG00000196415.
CleanExiHS_PRTN3.
ExpressionAtlasiP24158. baseline and differential.
GenevisibleiP24158. HS.

Organism-specific databases

HPAiCAB017558.
HPA005938.

Interactioni

GO - Molecular functioni

  • enzyme binding Source: UniProtKB

Protein-protein interaction databases

BioGridi111638. 6 interactors.
DIPiDIP-31107N.
IntActiP24158. 2 interactors.
MINTiMINT-4054660.
STRINGi9606.ENSP00000234347.

Chemistry databases

BindingDBiP24158.

Structurei

Secondary structure

1256
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi42 – 47Combined sources6
Beta strandi56 – 62Combined sources7
Beta strandi65 – 68Combined sources4
Helixi70 – 73Combined sources4
Beta strandi74 – 76Combined sources3
Helixi78 – 80Combined sources3
Beta strandi81 – 86Combined sources6
Beta strandi98 – 107Combined sources10
Turni112 – 115Combined sources4
Beta strandi120 – 126Combined sources7
Beta strandi151 – 160Combined sources10
Beta strandi162 – 164Combined sources3
Beta strandi171 – 178Combined sources8
Beta strandi186 – 190Combined sources5
Beta strandi192 – 195Combined sources4
Beta strandi206 – 209Combined sources4
Beta strandi212 – 219Combined sources8
Beta strandi221 – 223Combined sources3
Beta strandi227 – 229Combined sources3
Beta strandi231 – 235Combined sources5
Helixi236 – 239Combined sources4
Helixi240 – 247Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FUJX-ray2.20A/B/C/D28-248[»]
ProteinModelPortaliP24158.
SMRiP24158.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24158.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 248Peptidase S1PROSITE-ProRule annotationAdd BLAST221

Sequence similaritiesi

Belongs to the peptidase S1 family. Elastase subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118895.
HOVERGENiHBG013304.
InParanoidiP24158.
KOiK01350.
OMAiFLCRPHN.
OrthoDBiEOG091G0DF7.
PhylomeDBiP24158.
TreeFamiTF335284.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P24158-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHRPPSPAL ASVLLALLLS GAARAAEIVG GHEAQPHSRP YMASLQMRGN
60 70 80 90 100
PGSHFCGGTL IHPSFVLTAA HCLRDIPQRL VNVVLGAHNV RTQEPTQQHF
110 120 130 140 150
SVAQVFLNNY DAENKLNDVL LIQLSSPANL SASVATVQLP QQDQPVPHGT
160 170 180 190 200
QCLAMGWGRV GAHDPPAQVL QELNVTVVTF FCRPHNICTF VPRRKAGICF
210 220 230 240 250
GDSGGPLICD GIIQGIDSFV IWGCATRLFP DFFTRVALYV DWIRSTLRRV

EAKGRP
Length:256
Mass (Da):27,807
Last modified:December 15, 1998 - v3
Checksum:iCBECA36D8C4B2A40
GO

Sequence cautioni

The sequence AAA36342 differs from that shown. Reason: Frameshift at positions 34 and 39.Curated
The sequence CAA39598 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2A → R in CAA39203 (PubMed:2258701).Curated1
Sequence conflicti38S → I AA sequence (PubMed:1688612).Curated1
Sequence conflicti40P → PI AA sequence (PubMed:1688612).Curated1
Sequence conflicti46Q → E AA sequence (PubMed:2404977).Curated1
Sequence conflicti46Q → E AA sequence (PubMed:2501794).Curated1
Sequence conflicti48R → A AA sequence (PubMed:2121162).Curated1
Sequence conflicti64S → D AA sequence (PubMed:2033050).Curated1
Sequence conflicti70A → P in AAA59558 (PubMed:1681549).Curated1
Sequence conflicti255Missing in CAA39203 (PubMed:2258701).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_011691119V → I.7 PublicationsCorresponds to variant rs351111dbSNPEnsembl.1
Natural variantiVAR_011713135A → T.4 PublicationsCorresponds to variant rs1042281dbSNPEnsembl.1
Natural variantiVAR_011714136T → S.4 PublicationsCorresponds to variant rs1042282dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M75154 mRNA. Translation: AAA59558.1.
AC004799 Genomic DNA. No translation available.
CH471139 Genomic DNA. Translation: EAW69591.1.
BC096183 mRNA. Translation: AAH96183.1.
BC096184 mRNA. Translation: AAH96184.1.
BC096185 mRNA. Translation: AAH96185.1.
BC096186 mRNA. Translation: AAH96186.1.
M96628 Genomic DNA. Translation: AAB59364.1.
AH005293 Genomic DNA. Translation: AAB59493.1.
M97911 Genomic DNA. No translation available.
AH007523 Genomic DNA. Translation: AAD21524.1.
X56606 mRNA. Translation: CAA39943.1.
X55668 mRNA. Translation: CAA39203.1.
M29142 mRNA. Translation: AAA36342.1. Frameshift.
X56132 mRNA. Translation: CAA39597.1.
X56132 mRNA. Translation: CAA39598.1. Different initiation.
CCDSiCCDS32860.1.
PIRiA45080. PRHU3.
RefSeqiNP_002768.3. NM_002777.3.
UniGeneiHs.928.

Genome annotation databases

EnsembliENST00000234347; ENSP00000234347; ENSG00000196415.
ENST00000612112; ENSP00000478977; ENSG00000277804.
GeneIDi5657.
KEGGihsa:5657.
UCSCiuc002lqa.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Wikipedia

Proteinase 3 entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M75154 mRNA. Translation: AAA59558.1.
AC004799 Genomic DNA. No translation available.
CH471139 Genomic DNA. Translation: EAW69591.1.
BC096183 mRNA. Translation: AAH96183.1.
BC096184 mRNA. Translation: AAH96184.1.
BC096185 mRNA. Translation: AAH96185.1.
BC096186 mRNA. Translation: AAH96186.1.
M96628 Genomic DNA. Translation: AAB59364.1.
AH005293 Genomic DNA. Translation: AAB59493.1.
M97911 Genomic DNA. No translation available.
AH007523 Genomic DNA. Translation: AAD21524.1.
X56606 mRNA. Translation: CAA39943.1.
X55668 mRNA. Translation: CAA39203.1.
M29142 mRNA. Translation: AAA36342.1. Frameshift.
X56132 mRNA. Translation: CAA39597.1.
X56132 mRNA. Translation: CAA39598.1. Different initiation.
CCDSiCCDS32860.1.
PIRiA45080. PRHU3.
RefSeqiNP_002768.3. NM_002777.3.
UniGeneiHs.928.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FUJX-ray2.20A/B/C/D28-248[»]
ProteinModelPortaliP24158.
SMRiP24158.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111638. 6 interactors.
DIPiDIP-31107N.
IntActiP24158. 2 interactors.
MINTiMINT-4054660.
STRINGi9606.ENSP00000234347.

Chemistry databases

BindingDBiP24158.
ChEMBLiCHEMBL3900.
GuidetoPHARMACOLOGYi2401.

Protein family/group databases

MEROPSiS01.134.

Polymorphism and mutation databases

BioMutaiPRTN3.
DMDMi6174926.

Proteomic databases

EPDiP24158.
PaxDbiP24158.
PeptideAtlasiP24158.
PRIDEiP24158.

Protocols and materials databases

DNASUi5657.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000234347; ENSP00000234347; ENSG00000196415.
ENST00000612112; ENSP00000478977; ENSG00000277804.
GeneIDi5657.
KEGGihsa:5657.
UCSCiuc002lqa.2. human.

Organism-specific databases

CTDi5657.
DisGeNETi5657.
GeneCardsiPRTN3.
HGNCiHGNC:9495. PRTN3.
HPAiCAB017558.
HPA005938.
MalaCardsiPRTN3.
MIMi177020. gene.
neXtProtiNX_P24158.
OpenTargetsiENSG00000196415.
ENSG00000277804.
Orphaneti900. Granulomatosis with polyangiitis.
PharmGKBiPA33842.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118895.
HOVERGENiHBG013304.
InParanoidiP24158.
KOiK01350.
OMAiFLCRPHN.
OrthoDBiEOG091G0DF7.
PhylomeDBiP24158.
TreeFamiTF335284.

Enzyme and pathway databases

BioCyciZFISH:HS10473-MONOMER.
BRENDAi3.4.21.76. 2681.
ReactomeiR-HSA-1222556. ROS, RNS production in response to bacteria.
R-HSA-140875. Common Pathway of Fibrin Clot Formation.
R-HSA-449147. Signaling by Interleukins.
R-HSA-6798695. Neutrophil degranulation.
R-HSA-6803157. Antimicrobial peptides.

Miscellaneous databases

EvolutionaryTraceiP24158.
GeneWikiiProteinase_3.
GenomeRNAii5657.
PROiP24158.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000196415.
CleanExiHS_PRTN3.
ExpressionAtlasiP24158. baseline and differential.
GenevisibleiP24158. HS.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRTN3_HUMAN
AccessioniPrimary (citable) accession number: P24158
Secondary accession number(s): P15637
, P18078, Q4VB08, Q4VB09, Q6LBM7, Q6LBN2, Q9UD25, Q9UQD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: December 15, 1998
Last modified: November 30, 2016
This is version 183 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Peptidase families
    Classification of peptidase families and list of entries
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.