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P24155

- THOP1_RAT

UniProt

P24155 - THOP1_RAT

Protein

Thimet oligopeptidase

Gene

Thop1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 4 (26 Feb 2008)
      Previous versions | rss
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    Functioni

    Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation.

    Catalytic activityi

    Preferential cleavage of bonds with hydrophobic residues at P1, P2 and P3' and a small residue at P1' in substrates of 5 to 15 residues.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi473 – 4731Zinc; catalyticPROSITE-ProRule annotation
    Active sitei474 – 4741PROSITE-ProRule annotation
    Metal bindingi477 – 4771Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi480 – 4801Zinc; catalyticPROSITE-ProRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. metalloendopeptidase activity Source: RGD
    3. peptidase activity Source: RGD
    4. peptide binding Source: RGD
    5. protein binding Source: IntAct

    GO - Biological processi

    1. peptide metabolic process Source: Ensembl
    2. proteolysis Source: RGD

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BRENDAi3.4.24.15. 5301.

    Protein family/group databases

    MEROPSiM03.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thimet oligopeptidase (EC:3.4.24.15)
    Alternative name(s):
    Endo-oligopeptidase A
    Endopeptidase 24.15
    PZ-peptidase
    Soluble metallo-endopeptidase
    Gene namesi
    Name:Thop1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 7

    Organism-specific databases

    RGDi68330. Thop1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 687687Thimet oligopeptidasePRO_0000078155Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei257 – 2571N6-acetyllysineBy similarity
    Modified residuei278 – 2781PhosphotyrosineBy similarity
    Modified residuei538 – 5381N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP24155.
    PRIDEiP24155.

    PTM databases

    PhosphoSiteiP24155.

    Expressioni

    Tissue specificityi

    Expressed abundantly in the testis. It is also found in the liver, lung and kidney.

    Gene expression databases

    GenevestigatoriP24155.

    Interactioni

    Subunit structurei

    Monomer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Calm3P621612EBI-6372841,EBI-397530

    Protein-protein interaction databases

    BioGridi249102. 1 interaction.
    IntActiP24155. 2 interactions.
    STRINGi10116.ENSRNOP00000027045.

    Structurei

    3D structure databases

    ProteinModelPortaliP24155.
    SMRiP24155. Positions 24-677.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M3 family.Curated

    Phylogenomic databases

    eggNOGiCOG0339.
    GeneTreeiENSGT00550000074738.
    HOGENOMiHOG000245985.
    HOVERGENiHBG000238.
    InParanoidiQ66HS4.
    KOiK01392.
    OMAiGCEPPAC.
    OrthoDBiEOG7SR4KW.
    PhylomeDBiP24155.
    TreeFamiTF300459.

    Family and domain databases

    Gene3Di1.10.1370.10. 2 hits.
    1.20.1050.40. 1 hit.
    3.40.390.10. 1 hit.
    InterProiIPR024079. MetalloPept_cat_dom.
    IPR024077. Neurolysin/TOP_dom2.
    IPR024080. Neurolysin/TOP_N.
    IPR001567. Pept_M3A_M3B.
    [Graphical view]
    PfamiPF01432. Peptidase_M3. 1 hit.
    [Graphical view]
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24155-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKPPAACAGD VVDTVSPCST VNHLRWDLSA QQIRALTTQL IEQTKCVYDR    50
    VGAQDFEDVS YESTLKALAD VEVTYTVQRN ILDFPQHVSP NKDIRAASTE 100
    ADKKLSEFDV EMSMRQDVYQ RVVWLQEKIP KDSLKPEAAR YLERLIKLGR 150
    RNGLHLPQDT QEKIKNIKKR LSLLCIDFNK NLNEDTTFLP FTREELGGLP 200
    EDFLNSLEKT EDGKLKVTLK YPHYFPLLKK CHVPETRRLL EEAFNCRCKE 250
    ENCAILKELV SLRAQKSNLL GFRTHADYVL EMNMAKTSQT VATFLDELAR 300
    KLKPLGEQER AVILELKEAE CAKRGLPFDG RIHAWDMRYY MNQVEETRYR 350
    VDQNLLKEYF PMQVVTRGLL AIYQELLGLT FTLEEGAAAW HEDVRLYSVR 400
    DAASGEEIGK FYLDLYPREG KYGHAACFGL QPGCLRQDGS RQLAIAAMVA 450
    NFTKPTPDVP SLLQHDEVET YFHEFGHVMH QLCSQAEFAM FSGTHVERDF 500
    VEAPSQMLEN WVWEKEPLMR MSQHYRTGGE APEDLLEKLI KSRQANAGLF 550
    NLRQIVLAKV DQVLHTQTDV DPAEEYARLC QEILGVPATP GTNMPATFGH 600
    LAGGYDAQYY GYLWSEVYSM DMFHTRFKQE GVLSPKVGMD YRTSILRPGG 650
    SEDASTMLKQ FLGRDPKQDA FLLSKGLQVE GCEPPAC 687
    Length:687
    Mass (Da):78,385
    Last modified:February 26, 2008 - v4
    Checksum:iA0B399AF61B60F81
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti321 – 3211C → S in AAA41586. (PubMed:2261476)Curated
    Sequence conflicti347 – 3482TR → DS in AAA41586. (PubMed:2261476)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M61142 mRNA. Translation: AAA41586.1.
    BC081706 mRNA. Translation: AAH81706.1.
    PIRiS38760. HYRTTH.
    RefSeqiNP_742072.2. NM_172075.2.
    UniGeneiRn.9490.

    Genome annotation databases

    EnsembliENSRNOT00000027045; ENSRNOP00000027045; ENSRNOG00000019924.
    GeneIDi64517.
    KEGGirno:64517.
    UCSCiRGD:68330. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M61142 mRNA. Translation: AAA41586.1 .
    BC081706 mRNA. Translation: AAH81706.1 .
    PIRi S38760. HYRTTH.
    RefSeqi NP_742072.2. NM_172075.2.
    UniGenei Rn.9490.

    3D structure databases

    ProteinModelPortali P24155.
    SMRi P24155. Positions 24-677.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 249102. 1 interaction.
    IntActi P24155. 2 interactions.
    STRINGi 10116.ENSRNOP00000027045.

    Protein family/group databases

    MEROPSi M03.001.

    PTM databases

    PhosphoSitei P24155.

    Proteomic databases

    PaxDbi P24155.
    PRIDEi P24155.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000027045 ; ENSRNOP00000027045 ; ENSRNOG00000019924 .
    GeneIDi 64517.
    KEGGi rno:64517.
    UCSCi RGD:68330. rat.

    Organism-specific databases

    CTDi 7064.
    RGDi 68330. Thop1.

    Phylogenomic databases

    eggNOGi COG0339.
    GeneTreei ENSGT00550000074738.
    HOGENOMi HOG000245985.
    HOVERGENi HBG000238.
    InParanoidi Q66HS4.
    KOi K01392.
    OMAi GCEPPAC.
    OrthoDBi EOG7SR4KW.
    PhylomeDBi P24155.
    TreeFami TF300459.

    Enzyme and pathway databases

    BRENDAi 3.4.24.15. 5301.

    Miscellaneous databases

    NextBioi 613318.
    PROi P24155.

    Gene expression databases

    Genevestigatori P24155.

    Family and domain databases

    Gene3Di 1.10.1370.10. 2 hits.
    1.20.1050.40. 1 hit.
    3.40.390.10. 1 hit.
    InterProi IPR024079. MetalloPept_cat_dom.
    IPR024077. Neurolysin/TOP_dom2.
    IPR024080. Neurolysin/TOP_N.
    IPR001567. Pept_M3A_M3B.
    [Graphical view ]
    Pfami PF01432. Peptidase_M3. 1 hit.
    [Graphical view ]
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and primary structure of rat testes metalloendopeptidase EC 3.4.24.15."
      Pierotti A., Dong K.-W., Glucksman M.J., Orlowski M., Roberts J.L.
      Biochemistry 29:10323-10329(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Testis.
    2. "Thimet oligopeptidase: similarity to 'soluble angiotensin II-binding protein' and some corrections to the published amino acid sequence of the rat testis enzyme."
      McKie N., Dando P.M., Rawlings N.D., Barrett A.J.
      Biochem. J. 295:57-60(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    4. Lubec G., Chen W.-Q.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 35-45; 267-273; 311-317; 351-357; 411-418 AND 560-578, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Hippocampus.

    Entry informationi

    Entry nameiTHOP1_RAT
    AccessioniPrimary (citable) accession number: P24155
    Secondary accession number(s): Q66HS4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: February 26, 2008
    Last modified: October 1, 2014
    This is version 118 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3