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P24155 (THOP1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Thimet oligopeptidase
EC=3.4.24.15
Alternative name(s):
Endo-oligopeptidase A
Endopeptidase 24.15
PZ-peptidase
Soluble metallo-endopeptidase
Gene names
Name:Thop1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length687 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation.

Catalytic activity

Preferential cleavage of bonds with hydrophobic residues at P1, P2 and P3' and a small residue at P1' in substrates of 5 to 15 residues.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Monomer.

Subcellular location

Cytoplasm.

Tissue specificity

Expressed abundantly in the testis. It is also found in the liver, lung and kidney.

Sequence similarities

Belongs to the peptidase M3 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Calm3P621612EBI-6372841,EBI-397530

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 687687Thimet oligopeptidase
PRO_0000078155

Sites

Active site4741 By similarity
Metal binding4731Zinc; catalytic By similarity
Metal binding4771Zinc; catalytic By similarity
Metal binding4801Zinc; catalytic By similarity

Amino acid modifications

Modified residue2571N6-acetyllysine By similarity
Modified residue2781Phosphotyrosine By similarity
Modified residue5381N6-acetyllysine By similarity

Experimental info

Sequence conflict3211C → S in AAA41586. Ref.1
Sequence conflict347 – 3482TR → DS in AAA41586. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P24155 [UniParc].

Last modified February 26, 2008. Version 4.
Checksum: A0B399AF61B60F81

FASTA68778,385
        10         20         30         40         50         60 
MKPPAACAGD VVDTVSPCST VNHLRWDLSA QQIRALTTQL IEQTKCVYDR VGAQDFEDVS 

        70         80         90        100        110        120 
YESTLKALAD VEVTYTVQRN ILDFPQHVSP NKDIRAASTE ADKKLSEFDV EMSMRQDVYQ 

       130        140        150        160        170        180 
RVVWLQEKIP KDSLKPEAAR YLERLIKLGR RNGLHLPQDT QEKIKNIKKR LSLLCIDFNK 

       190        200        210        220        230        240 
NLNEDTTFLP FTREELGGLP EDFLNSLEKT EDGKLKVTLK YPHYFPLLKK CHVPETRRLL 

       250        260        270        280        290        300 
EEAFNCRCKE ENCAILKELV SLRAQKSNLL GFRTHADYVL EMNMAKTSQT VATFLDELAR 

       310        320        330        340        350        360 
KLKPLGEQER AVILELKEAE CAKRGLPFDG RIHAWDMRYY MNQVEETRYR VDQNLLKEYF 

       370        380        390        400        410        420 
PMQVVTRGLL AIYQELLGLT FTLEEGAAAW HEDVRLYSVR DAASGEEIGK FYLDLYPREG 

       430        440        450        460        470        480 
KYGHAACFGL QPGCLRQDGS RQLAIAAMVA NFTKPTPDVP SLLQHDEVET YFHEFGHVMH 

       490        500        510        520        530        540 
QLCSQAEFAM FSGTHVERDF VEAPSQMLEN WVWEKEPLMR MSQHYRTGGE APEDLLEKLI 

       550        560        570        580        590        600 
KSRQANAGLF NLRQIVLAKV DQVLHTQTDV DPAEEYARLC QEILGVPATP GTNMPATFGH 

       610        620        630        640        650        660 
LAGGYDAQYY GYLWSEVYSM DMFHTRFKQE GVLSPKVGMD YRTSILRPGG SEDASTMLKQ 

       670        680 
FLGRDPKQDA FLLSKGLQVE GCEPPAC

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and primary structure of rat testes metalloendopeptidase EC 3.4.24.15."
Pierotti A., Dong K.-W., Glucksman M.J., Orlowski M., Roberts J.L.
Biochemistry 29:10323-10329(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Testis.
[2]"Thimet oligopeptidase: similarity to 'soluble angiotensin II-binding protein' and some corrections to the published amino acid sequence of the rat testis enzyme."
McKie N., Dando P.M., Rawlings N.D., Barrett A.J.
Biochem. J. 295:57-60(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[4]Lubec G., Chen W.-Q.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 35-45; 267-273; 311-317; 351-357; 411-418 AND 560-578, MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Hippocampus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M61142 mRNA. Translation: AAA41586.1.
BC081706 mRNA. Translation: AAH81706.1.
IPIIPI00211777.
PIRHYRTTH. S38760.
RefSeqNP_742072.2. NM_172075.2.
UniGeneRn.9490.

3D structure databases

ProteinModelPortalP24155.
SMRP24155. Positions 24-677.
ModBaseSearch...

Protein-protein interaction databases

IntActP24155. 2 interactions.
STRING10116.ENSRNOP00000027045.

Protein family/group databases

MEROPSM03.001.

PTM databases

PhosphoSiteP24155.

Proteomic databases

PaxDbP24155.
PRIDEP24155.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000027045; ENSRNOP00000027045; ENSRNOG00000019924.
GeneID64517.
KEGGrno:64517.
UCSCRGD:68330. rat.

Organism-specific databases

CTD7064.
RGD68330. Thop1.

Phylogenomic databases

eggNOGCOG0339.
GeneTreeENSGT00550000074738.
HOGENOMHOG000245985.
HOVERGENHBG000238.
InParanoidQ66HS4.
KOK01392.
OMAITFKERT.
OrthoDBEOG4CG07P.

Enzyme and pathway databases

BRENDA3.4.24.15. 5301.

Gene expression databases

GenevestigatorP24155.
GermOnlineENSRNOG00000019924. Rattus norvegicus.

Family and domain databases

Gene3D1.10.1370.10. 2 hits.
1.20.1050.40. 1 hit.
3.40.390.10. 1 hit.
InterProIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR024080. Neurolysin/TOP_N.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio613318.

Entry information

Entry nameTHOP1_RAT
AccessionPrimary (citable) accession number: P24155
Secondary accession number(s): Q66HS4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: February 26, 2008
Last modified: April 3, 2013
This is version 107 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

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