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P24146

- LEC4_GRISI

UniProt

P24146 - LEC4_GRISI

Protein

Lectin-4

Gene
N/A
Organism
Griffonia simplicifolia (Bandeiraea simplicifolia)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 3 (01 Jun 1994)
      Previous versions | rss
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    Functioni

    Lectin which has a strong affinity for both the Lewis b and y human blood-group determinants.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi129 – 1291Manganese
    Metal bindingi131 – 1311Calcium
    Metal bindingi131 – 1311Manganese
    Metal bindingi133 – 1331Calcium
    Metal bindingi135 – 1351Calcium
    Metal bindingi140 – 1401Calcium
    Metal bindingi140 – 1401Manganese
    Metal bindingi145 – 1451Manganese

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Ligandi

    Calcium, Lectin, Manganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lectin-4
    Alternative name(s):
    GS4
    Lectin IV
    OrganismiGriffonia simplicifolia (Bandeiraea simplicifolia)
    Taxonomic identifieri3850 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaeCaesalpinioideaeCercideaeGriffonia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 243243Lectin-4PRO_0000105099Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11Pyrrolidone carboxylic acid
    Glycosylationi5 – 51N-linked (GlcNAc...); in alpha chain
    Glycosylationi18 – 181N-linked (GlcNAc...)

    Post-translational modificationi

    N-glycosylation of Asn-5 converts form Beta to form Alpha.

    Keywords - PTMi

    Glycoprotein, Pyrrolidone carboxylic acid

    Interactioni

    Subunit structurei

    Homodimer of Alpha and Beta forms.

    Structurei

    Secondary structure

    1
    243
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 95
    Beta strandi20 – 267
    Beta strandi29 – 313
    Beta strandi34 – 363
    Beta strandi48 – 5811
    Beta strandi71 – 8616
    Beta strandi90 – 978
    Helixi106 – 1083
    Turni109 – 1113
    Turni114 – 1185
    Helixi120 – 1223
    Beta strandi126 – 1316
    Helixi136 – 1383
    Beta strandi145 – 15410
    Beta strandi156 – 1605
    Helixi163 – 1664
    Beta strandi171 – 1799
    Turni180 – 1834
    Beta strandi184 – 1918
    Beta strandi196 – 2027
    Helixi205 – 2084
    Beta strandi211 – 22111
    Beta strandi223 – 23715
    Turni239 – 2424

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1GSLX-ray2.00A2-243[»]
    1LECX-ray2.00A2-243[»]
    1LEDX-ray2.00A2-243[»]
    ProteinModelPortaliP24146.
    SMRiP24146. Positions 1-243.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP24146.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the leguminous lectin family.Curated

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR016363. Lectin.
    IPR000985. Lectin_LegA_CS.
    IPR019825. Lectin_legB_Mn/Ca_BS.
    IPR001220. Legume_lectin_dom.
    [Graphical view]
    PfamiPF00139. Lectin_legB. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002690. L-type_lectin_plant. 1 hit.
    SUPFAMiSSF49899. SSF49899. 1 hit.
    PROSITEiPS00308. LECTIN_LEGUME_ALPHA. 1 hit.
    PS00307. LECTIN_LEGUME_BETA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24146-1 [UniParc]FASTAAdd to Basket

    « Hide

    QNTVNFTYPD FWSYSLKNGT EITFLGDATR IPGALQLTKT DANGNPVRSS    50
    AGQASYSEPV FLWDSTGKAA SFYTSFTFLL KNYGAPTADG LAFFLAPVDS 100
    SVKDYGGFLG LFRHETAADP SKNQVVAVEF DTWINKDWND PPYPHIGIDV 150
    NSIVSVATTR WENDDAYGSS IATAHITYDA RSKILTVLLS YEHGRDYILS 200
    HVVDLAKVLP QKVRIGFSAG VGYDEVTYIL SWHFFSTLDG TNK 243
    Length:243
    Mass (Da):26,810
    Last modified:June 1, 1994 - v3
    Checksum:iCFEC0FA389BBBF25
    GO

    Sequence databases

    PIRiS13389.

    Cross-referencesi

    Sequence databases

    PIRi S13389.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1GSL X-ray 2.00 A 2-243 [» ]
    1LEC X-ray 2.00 A 2-243 [» ]
    1LED X-ray 2.00 A 2-243 [» ]
    ProteinModelPortali P24146.
    SMRi P24146. Positions 1-243.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P24146.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR016363. Lectin.
    IPR000985. Lectin_LegA_CS.
    IPR019825. Lectin_legB_Mn/Ca_BS.
    IPR001220. Legume_lectin_dom.
    [Graphical view ]
    Pfami PF00139. Lectin_legB. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002690. L-type_lectin_plant. 1 hit.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    PROSITEi PS00308. LECTIN_LEGUME_ALPHA. 1 hit.
    PS00307. LECTIN_LEGUME_BETA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structures of the lectin IV of Griffonia simplicifolia and its complex with the Lewis b human blood group determinant at 2.0-A resolution."
      Delbaere L.T.J., Vandonselaar M., Prasad L., Quail J.W., Wilson K.S., Dauter Z.
      J. Mol. Biol. 230:950-965(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
    2. "Molecular-mass heterogeneity of Griffonia simplicifolia lectin IV subunits. Differences in the oligosaccharide moieties in the N-terminal region."
      Nikrad P.V., Pearlstone J.R., Carpenter M.R., Lemieux R.U., Smillie L.B.
      Biochem. J. 272:343-350(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-62.
      Tissue: Seed.

    Entry informationi

    Entry nameiLEC4_GRISI
    AccessioniPrimary (citable) accession number: P24146
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: June 1, 1994
    Last modified: October 1, 2014
    This is version 86 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Binds one manganese (or another transition metal) ion and one calcium ion. The metal ions are essential for the saccharide-binding and cell-agglutinating activities.

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3