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P24146 (LEC4_GRISI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lectin-4
Alternative name(s):
GS4
Lectin IV
OrganismGriffonia simplicifolia (Bandeiraea simplicifolia)
Taxonomic identifier3850 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaeCaesalpinioideaeCercideaeGriffonia

Protein attributes

Sequence length243 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Lectin which has a strong affinity for both the Lewis b and y human blood-group determinants.

Subunit structure

Homodimer of Alpha and Beta forms.

Post-translational modification

N-glycosylation of Asn-5 converts form Beta to form Alpha.

Miscellaneous

Binds one manganese (or another transition metal) ion and one calcium ion. The metal ions are essential for the saccharide-binding and cell-agglutinating activities.

Sequence similarities

Belongs to the leguminous lectin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 243243Lectin-4
PRO_0000105099

Sites

Metal binding1291Manganese
Metal binding1311Calcium
Metal binding1311Manganese
Metal binding1331Calcium
Metal binding1351Calcium
Metal binding1401Calcium
Metal binding1401Manganese
Metal binding1451Manganese

Amino acid modifications

Modified residue11Pyrrolidone carboxylic acid
Glycosylation51N-linked (GlcNAc...); in alpha chain
Glycosylation181N-linked (GlcNAc...)

Secondary structure

.............................................. 243
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P24146 [UniParc].

Last modified June 1, 1994. Version 3.
Checksum: CFEC0FA389BBBF25

FASTA24326,810
        10         20         30         40         50         60 
QNTVNFTYPD FWSYSLKNGT EITFLGDATR IPGALQLTKT DANGNPVRSS AGQASYSEPV 

        70         80         90        100        110        120 
FLWDSTGKAA SFYTSFTFLL KNYGAPTADG LAFFLAPVDS SVKDYGGFLG LFRHETAADP 

       130        140        150        160        170        180 
SKNQVVAVEF DTWINKDWND PPYPHIGIDV NSIVSVATTR WENDDAYGSS IATAHITYDA 

       190        200        210        220        230        240 
RSKILTVLLS YEHGRDYILS HVVDLAKVLP QKVRIGFSAG VGYDEVTYIL SWHFFSTLDG 


TNK 

« Hide

References

[1]"Structures of the lectin IV of Griffonia simplicifolia and its complex with the Lewis b human blood group determinant at 2.0-A resolution."
Delbaere L.T.J., Vandonselaar M., Prasad L., Quail J.W., Wilson K.S., Dauter Z.
J. Mol. Biol. 230:950-965(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[2]"Molecular-mass heterogeneity of Griffonia simplicifolia lectin IV subunits. Differences in the oligosaccharide moieties in the N-terminal region."
Nikrad P.V., Pearlstone J.R., Carpenter M.R., Lemieux R.U., Smillie L.B.
Biochem. J. 272:343-350(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-62.
Tissue: Seed.

Cross-references

Sequence databases

PIRS13389.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GSLX-ray2.00A2-243[»]
1LECX-ray2.00A2-243[»]
1LEDX-ray2.00A2-243[»]
ProteinModelPortalP24146.
SMRP24146. Positions 1-243.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR016363. Lectin.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view]
PfamPF00139. Lectin_legB. 1 hit.
[Graphical view]
PIRSFPIRSF002690. L-type_lectin_plant. 1 hit.
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP24146.

Entry information

Entry nameLEC4_GRISI
AccessionPrimary (citable) accession number: P24146
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: June 1, 1994
Last modified: February 19, 2014
This is version 85 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references