Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P24146

- LEC4_GRISI

UniProt

P24146 - LEC4_GRISI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Lectin-4

Gene
N/A
Organism
Griffonia simplicifolia (Bandeiraea simplicifolia)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Lectin which has a strong affinity for both the Lewis b and y human blood-group determinants.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi129 – 1291Manganese
Metal bindingi131 – 1311Calcium
Metal bindingi131 – 1311Manganese
Metal bindingi133 – 1331Calcium
Metal bindingi135 – 1351Calcium
Metal bindingi140 – 1401Calcium
Metal bindingi140 – 1401Manganese
Metal bindingi145 – 1451Manganese

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

Calcium, Lectin, Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lectin-4
Alternative name(s):
GS4
Lectin IV
OrganismiGriffonia simplicifolia (Bandeiraea simplicifolia)
Taxonomic identifieri3850 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaeCaesalpinioideaeCercideaeGriffonia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 243243Lectin-4PRO_0000105099Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11Pyrrolidone carboxylic acid
Glycosylationi5 – 51N-linked (GlcNAc...); in alpha chain
Glycosylationi18 – 181N-linked (GlcNAc...)

Post-translational modificationi

N-glycosylation of Asn-5 converts form Beta to form Alpha.

Keywords - PTMi

Glycoprotein, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Homodimer of Alpha and Beta forms.

Structurei

Secondary structure

1
243
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95
Beta strandi20 – 267
Beta strandi29 – 313
Beta strandi34 – 363
Beta strandi48 – 5811
Beta strandi71 – 8616
Beta strandi90 – 978
Helixi106 – 1083
Turni109 – 1113
Turni114 – 1185
Helixi120 – 1223
Beta strandi126 – 1316
Helixi136 – 1383
Beta strandi145 – 15410
Beta strandi156 – 1605
Helixi163 – 1664
Beta strandi171 – 1799
Turni180 – 1834
Beta strandi184 – 1918
Beta strandi196 – 2027
Helixi205 – 2084
Beta strandi211 – 22111
Beta strandi223 – 23715
Turni239 – 2424

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GSLX-ray2.00A2-243[»]
1LECX-ray2.00A2-243[»]
1LEDX-ray2.00A2-243[»]
ProteinModelPortaliP24146.
SMRiP24146. Positions 1-243.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24146.

Family & Domainsi

Sequence similaritiesi

Belongs to the leguminous lectin family.Curated

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR016363. Lectin.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view]
PfamiPF00139. Lectin_legB. 1 hit.
[Graphical view]
PIRSFiPIRSF002690. L-type_lectin_plant. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiPS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24146-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
QNTVNFTYPD FWSYSLKNGT EITFLGDATR IPGALQLTKT DANGNPVRSS
60 70 80 90 100
AGQASYSEPV FLWDSTGKAA SFYTSFTFLL KNYGAPTADG LAFFLAPVDS
110 120 130 140 150
SVKDYGGFLG LFRHETAADP SKNQVVAVEF DTWINKDWND PPYPHIGIDV
160 170 180 190 200
NSIVSVATTR WENDDAYGSS IATAHITYDA RSKILTVLLS YEHGRDYILS
210 220 230 240
HVVDLAKVLP QKVRIGFSAG VGYDEVTYIL SWHFFSTLDG TNK
Length:243
Mass (Da):26,810
Last modified:June 1, 1994 - v3
Checksum:iCFEC0FA389BBBF25
GO

Sequence databases

PIRiS13389.

Cross-referencesi

Sequence databases

PIRi S13389.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1GSL X-ray 2.00 A 2-243 [» ]
1LEC X-ray 2.00 A 2-243 [» ]
1LED X-ray 2.00 A 2-243 [» ]
ProteinModelPortali P24146.
SMRi P24146. Positions 1-243.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Miscellaneous databases

EvolutionaryTracei P24146.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR013320. ConA-like_dom.
IPR016363. Lectin.
IPR000985. Lectin_LegA_CS.
IPR019825. Lectin_legB_Mn/Ca_BS.
IPR001220. Legume_lectin_dom.
[Graphical view ]
Pfami PF00139. Lectin_legB. 1 hit.
[Graphical view ]
PIRSFi PIRSF002690. L-type_lectin_plant. 1 hit.
SUPFAMi SSF49899. SSF49899. 1 hit.
PROSITEi PS00308. LECTIN_LEGUME_ALPHA. 1 hit.
PS00307. LECTIN_LEGUME_BETA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structures of the lectin IV of Griffonia simplicifolia and its complex with the Lewis b human blood group determinant at 2.0-A resolution."
    Delbaere L.T.J., Vandonselaar M., Prasad L., Quail J.W., Wilson K.S., Dauter Z.
    J. Mol. Biol. 230:950-965(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
  2. "Molecular-mass heterogeneity of Griffonia simplicifolia lectin IV subunits. Differences in the oligosaccharide moieties in the N-terminal region."
    Nikrad P.V., Pearlstone J.R., Carpenter M.R., Lemieux R.U., Smillie L.B.
    Biochem. J. 272:343-350(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-62.
    Tissue: Seed.

Entry informationi

Entry nameiLEC4_GRISI
AccessioniPrimary (citable) accession number: P24146
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: June 1, 1994
Last modified: October 29, 2014
This is version 87 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds one manganese (or another transition metal) ion and one calcium ion. The metal ions are essential for the saccharide-binding and cell-agglutinating activities.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3