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P24140

- GPT_CRIGR

UniProt

P24140 - GPT_CRIGR

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Protein

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Gene
DPAGT1, DPAGT2, GNPTA, GTR2
Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

Enzyme regulationi

Enzyme activity is stimulated by phosphatidylglycerol and mannosylphosphoryldolichol and inhibited by tunicamycin.

Pathwayi

GO - Molecular functioni

  1. phospho-N-acetylmuramoyl-pentapeptide-transferase activity Source: InterPro
  2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW
  3. UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity Source: UniProtKB

GO - Biological processi

  1. dolichol biosynthetic process Source: UniProtKB
  2. protein glycosylation Source: UniProtKB-UniPathway
  3. protein oligomerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC:2.7.8.15)
Alternative name(s):
GlcNAc-1-P transferase
Short name:
G1PT
Short name:
GPT
N-acetylglucosamine-1-phosphate transferase
Gene namesi
Name:DPAGT1
Synonyms:DPAGT2, GNPTA, GTR2
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66Lumenal Reviewed prediction
Transmembranei7 – 3226Helical; Reviewed predictionAdd
BLAST
Topological domaini33 – 5725Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei58 – 7922Helical; Reviewed predictionAdd
BLAST
Topological domaini80 – 9415Lumenal Reviewed predictionAdd
BLAST
Transmembranei95 – 11420Helical; Reviewed predictionAdd
BLAST
Topological domaini115 – 12511Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei126 – 14520Helical; Reviewed predictionAdd
BLAST
Topological domaini146 – 16419Lumenal Reviewed predictionAdd
BLAST
Transmembranei165 – 18420Helical; Reviewed predictionAdd
BLAST
Topological domaini185 – 19410Cytoplasmic Reviewed prediction
Transmembranei195 – 21117Helical; Reviewed predictionAdd
BLAST
Topological domaini212 – 22110Lumenal Reviewed prediction
Transmembranei222 – 24019Helical; Reviewed predictionAdd
BLAST
Topological domaini241 – 25212Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei253 – 26917Helical; Reviewed predictionAdd
BLAST
Topological domaini270 – 2745Lumenal Reviewed prediction
Transmembranei275 – 29420Helical; Reviewed predictionAdd
BLAST
Topological domaini295 – 37884Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei379 – 39719Helical; Reviewed predictionAdd
BLAST
Topological domaini398 – 40811Lumenal Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB
  2. integral component of endoplasmic reticulum membrane Source: UniProtKB
  3. integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 408408UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferasePRO_0000108760Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi146 – 1461N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi67 – 7913Dolichol recognitionAdd
BLAST
Motifi222 – 23413Dolichol recognitionAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG000846.
KOiK01001.

Family and domain databases

InterProiIPR000715. Glycosyl_transferase_4.
[Graphical view]
PfamiPF00953. Glycos_transf_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24140-1 [UniParc]FASTAAdd to Basket

« Hide

MWAFPELPLP LLVNLFGSLL GFVATVTLIP AFRSHFIAAR LCGQDLNKLS    50
RQQIPESQGV ICGAVFLIIL FCFIPFPFLN CFVEEQCKAF PHHEFVALIG 100
ALLAICCMIF LGFADDVLNL RWRHKLLLPT AASLPLLMVY FTNFGNTTIV 150
VPKPFRWILG LHLDLGILYY VYMGLLAVFC TNAINILAGI NGLEAGQSLV 200
ISASIIVFNL VELEGDYRDD HVFSLYFMIP FFFTTLGLLY HNWYPSQVFV 250
GDTFCYFAGM TFAVVGILGH FSKTMLLFFI PQVFNFLYSL PQLLHAIPCP 300
RHRIPRLNPK TGKLEMSYSK FKTKNLSFLG TFILKVAERL QLVTVHRGES 350
EDGAFTECNN MTLINLLLKI FGPIHERNLT LLLLLLQILS SAVTFSIRYQ 400
LVRLFYDV 408
Length:408
Mass (Da):46,250
Last modified:March 1, 1992 - v1
Checksum:iCB0FF5946C07BEF3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36899 mRNA. Translation: AAA37027.1.
U15161 Genomic DNA. Translation: AAA51872.1.
U09453 Genomic DNA. Translation: AAA85220.1.
RefSeqiNP_001230970.1. NM_001244041.1.

Genome annotation databases

GeneIDi100689054.
KEGGicge:100689054.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36899 mRNA. Translation: AAA37027.1 .
U15161 Genomic DNA. Translation: AAA51872.1 .
U09453 Genomic DNA. Translation: AAA85220.1 .
RefSeqi NP_001230970.1. NM_001244041.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100689054.
KEGGi cge:100689054.

Organism-specific databases

CTDi 1798.

Phylogenomic databases

HOVERGENi HBG000846.
KOi K01001.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Family and domain databases

InterProi IPR000715. Glycosyl_transferase_4.
[Graphical view ]
Pfami PF00953. Glycos_transf_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Sequence of a cDNA that specifies the uridine diphosphate N-acetyl-D-glucosamine:dolichol phosphate N-acetylglucosamine-1-phosphate transferase from Chinese hamster ovary cells."
    Scocca J.R., Krag S.S.
    J. Biol. Chem. 265:20621-20626(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.
  2. "Genomic organization and expression of hamster UDP-N-acetylglucosamine:dolichyl phosphate N-acetylglucosaminyl phosphoryl transferase."
    Scocca J.R., Zou J., Krag S.S.
    Glycobiology 5:129-136(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

Entry informationi

Entry nameiGPT_CRIGR
AccessioniPrimary (citable) accession number: P24140
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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