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Protein

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Gene

DPAGT1

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

Enzyme regulationi

Enzyme activity is stimulated by phosphatidylglycerol and mannosylphosphoryldolichol and inhibited by tunicamycin.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • dolichol biosynthetic process Source: UniProtKB
  • dolichol-linked oligosaccharide biosynthetic process Source: InterPro
  • protein N-linked glycosylation Source: InterPro
  • protein oligomerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC:2.7.8.15)
Alternative name(s):
GlcNAc-1-P transferase
Short name:
G1PT
Short name:
GPT
N-acetylglucosamine-1-phosphate transferase
Gene namesi
Name:DPAGT1
Synonyms:DPAGT2, GNPTA, GTR2
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6LumenalSequence analysis6
Transmembranei7 – 32HelicalSequence analysisAdd BLAST26
Topological domaini33 – 57CytoplasmicSequence analysisAdd BLAST25
Transmembranei58 – 79HelicalSequence analysisAdd BLAST22
Topological domaini80 – 94LumenalSequence analysisAdd BLAST15
Transmembranei95 – 114HelicalSequence analysisAdd BLAST20
Topological domaini115 – 125CytoplasmicSequence analysisAdd BLAST11
Transmembranei126 – 145HelicalSequence analysisAdd BLAST20
Topological domaini146 – 164LumenalSequence analysisAdd BLAST19
Transmembranei165 – 184HelicalSequence analysisAdd BLAST20
Topological domaini185 – 194CytoplasmicSequence analysis10
Transmembranei195 – 211HelicalSequence analysisAdd BLAST17
Topological domaini212 – 221LumenalSequence analysis10
Transmembranei222 – 240HelicalSequence analysisAdd BLAST19
Topological domaini241 – 252CytoplasmicSequence analysisAdd BLAST12
Transmembranei253 – 269HelicalSequence analysisAdd BLAST17
Topological domaini270 – 274LumenalSequence analysis5
Transmembranei275 – 294HelicalSequence analysisAdd BLAST20
Topological domaini295 – 378CytoplasmicSequence analysisAdd BLAST84
Transmembranei379 – 397HelicalSequence analysisAdd BLAST19
Topological domaini398 – 408LumenalSequence analysisAdd BLAST11

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: UniProtKB
  • integral component of endoplasmic reticulum membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001087601 – 408UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferaseAdd BLAST408

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi146N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi67 – 79Dolichol recognitionAdd BLAST13
Motifi222 – 234Dolichol recognitionAdd BLAST13

Sequence similaritiesi

Belongs to the glycosyltransferase 4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG000846.
KOiK01001.

Family and domain databases

CDDicd06855. GT_GPT_euk. 1 hit.
InterProiIPR000715. Glycosyl_transferase_4.
IPR033895. GPT.
[Graphical view]
PANTHERiPTHR10571. PTHR10571. 1 hit.
PfamiPF00953. Glycos_transf_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24140-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWAFPELPLP LLVNLFGSLL GFVATVTLIP AFRSHFIAAR LCGQDLNKLS
60 70 80 90 100
RQQIPESQGV ICGAVFLIIL FCFIPFPFLN CFVEEQCKAF PHHEFVALIG
110 120 130 140 150
ALLAICCMIF LGFADDVLNL RWRHKLLLPT AASLPLLMVY FTNFGNTTIV
160 170 180 190 200
VPKPFRWILG LHLDLGILYY VYMGLLAVFC TNAINILAGI NGLEAGQSLV
210 220 230 240 250
ISASIIVFNL VELEGDYRDD HVFSLYFMIP FFFTTLGLLY HNWYPSQVFV
260 270 280 290 300
GDTFCYFAGM TFAVVGILGH FSKTMLLFFI PQVFNFLYSL PQLLHAIPCP
310 320 330 340 350
RHRIPRLNPK TGKLEMSYSK FKTKNLSFLG TFILKVAERL QLVTVHRGES
360 370 380 390 400
EDGAFTECNN MTLINLLLKI FGPIHERNLT LLLLLLQILS SAVTFSIRYQ

LVRLFYDV
Length:408
Mass (Da):46,250
Last modified:March 1, 1992 - v1
Checksum:iCB0FF5946C07BEF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36899 mRNA. Translation: AAA37027.1.
U15161 Genomic DNA. Translation: AAA51872.1.
U09453 Genomic DNA. Translation: AAA85220.1.
RefSeqiNP_001230970.1. NM_001244041.1.

Genome annotation databases

GeneIDi100689054.
KEGGicge:100689054.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36899 mRNA. Translation: AAA37027.1.
U15161 Genomic DNA. Translation: AAA51872.1.
U09453 Genomic DNA. Translation: AAA85220.1.
RefSeqiNP_001230970.1. NM_001244041.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100689054.
KEGGicge:100689054.

Organism-specific databases

CTDi1798.

Phylogenomic databases

HOVERGENiHBG000846.
KOiK01001.

Enzyme and pathway databases

UniPathwayiUPA00378.

Family and domain databases

CDDicd06855. GT_GPT_euk. 1 hit.
InterProiIPR000715. Glycosyl_transferase_4.
IPR033895. GPT.
[Graphical view]
PANTHERiPTHR10571. PTHR10571. 1 hit.
PfamiPF00953. Glycos_transf_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGPT_CRIGR
AccessioniPrimary (citable) accession number: P24140
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 30, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.