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P24135

- PLCG2_RAT

UniProt

P24135 - PLCG2_RAT

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2

Gene

Plcg2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 1 (01 Mar 1992)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Cofactori

    Calcium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei327 – 3271PROSITE-ProRule annotation
    Active sitei372 – 3721PROSITE-ProRule annotation

    GO - Molecular functioni

    1. phosphatidylinositol phospholipase C activity Source: RGD
    2. protein binding Source: RGD
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. phosphatidylinositol biosynthetic process Source: UniProtKB
    3. phospholipid catabolic process Source: InterPro
    4. positive regulation of cytosolic calcium ion concentration Source: RGD
    5. release of sequestered calcium ion into cytosol Source: UniProtKB
    6. response to ATP Source: RGD
    7. response to magnesium ion Source: RGD

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium

    Enzyme and pathway databases

    ReactomeiREACT_203734. FCERI mediated Ca+2 mobilization.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase C-gamma-2
    Phospholipase C-IV
    Short name:
    PLC-IV
    Phospholipase C-gamma-2
    Short name:
    PLC-gamma-2
    Gene namesi
    Name:Plcg2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3348. Plcg2.

    Subcellular locationi

    GO - Cellular componenti

    1. membrane Source: RGD
    2. plasma membrane Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 126512651-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2PRO_0000088502Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei753 – 7531Phosphotyrosine; by BTKBy similarity
    Modified residuei759 – 7591Phosphotyrosine; by BTKBy similarity
    Modified residuei1197 – 11971Phosphotyrosine; by BTK1 Publication
    Modified residuei1217 – 12171PhosphotyrosineBy similarity
    Modified residuei1245 – 12451PhosphotyrosineBy similarity

    Post-translational modificationi

    Phosphorylated on tyrosine residues by CSF1R By similarity. Phosphorylated on tyrosine residues by BTK and SYK; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Phosphorylation leads to increased phospholipase activity By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP24135.
    PRIDEiP24135.

    PTM databases

    PhosphoSiteiP24135.

    Expressioni

    Gene expression databases

    GenevestigatoriP24135.

    Interactioni

    Subunit structurei

    Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated).By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000018678.

    Structurei

    Secondary structure

    1
    1265
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Turni641 – 6433
    Beta strandi647 – 6504
    Helixi653 – 66210
    Beta strandi665 – 6728
    Beta strandi680 – 6867
    Beta strandi689 – 70012
    Beta strandi702 – 7076
    Beta strandi710 – 7123
    Helixi713 – 7208
    Beta strandi725 – 7284
    Helixi737 – 7426

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2EOBNMR-A633-743[»]
    ProteinModelPortaliP24135.
    SMRiP24135. Positions 520-744, 772-829.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP24135.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 131112PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini312 – 456145PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini532 – 635104SH2 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini646 – 73590SH2 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini769 – 82961SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini930 – 1044115PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini1059 – 115294C2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation
    Contains 2 SH2 domains.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, SH2 domain, SH3 domain

    Phylogenomic databases

    eggNOGiNOG268751.
    HOGENOMiHOG000230864.
    HOVERGENiHBG053611.
    InParanoidiP24135.
    KOiK05859.
    PhylomeDBiP24135.

    Family and domain databases

    Gene3Di2.30.29.30. 3 hits.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    3.30.505.10. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR016279. PLC-gamma.
    IPR028381. PLC-gamma2.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF25. PTHR10336:SF25. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    PF00017. SH2. 2 hits.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000952. PLC-gamma. 1 hit.
    PRINTSiPR00390. PHPHLIPASEC.
    PR00401. SH2DOMAIN.
    SMARTiSM00239. C2. 1 hit.
    SM00233. PH. 2 hits.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    SM00252. SH2. 2 hits.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF50044. SSF50044. 1 hit.
    SSF51695. SSF51695. 2 hits.
    SSF55550. SSF55550. 2 hits.
    PROSITEiPS50004. C2. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    PS50001. SH2. 2 hits.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24135-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTMVNVDTL PEYEKSQIKR ALELGTVMTV FSARKSTPER RTVQMIMETR     50
    QVAWSKTADK IEGFLDIMEI KEIRPGKNSK DFERAKAVRH KADCCFTIFY 100
    GTQFVLSTLS LATDSKEDAV KWLSGLKILH QEAMNASTPT MIESWLRKQI 150
    YSVDQTRRNS ISLRELKTIL PLVNFKVSGI KFLKDKLVEI GAQKDELSFE 200
    QFHLFYKKLM FEQQKSILDE FKKDSSVFIL GNTDRPDASA VYLQDFQRFL 250
    LHEQQELWAQ DLNKVRERMT KFIDDTMRET AEPFLFVDEF LTYLFSRENS 300
    IWDEKYDAVD MQDMNNPLSH YWISSSHNTY LTGDQLRSES STEAYIRCLR 350
    AGCRCIELDC WDGPDGKPII YHGWTRTTKI KFDDVVQAIR DHAFVTSSFP 400
    VILSIEEHCS VEQQRHMAKV FKEVLGDLLL TKPTEASADQ LPSPSQLREK 450
    IIIKHKKLGP RGDVDVNVED KKDEHKTQGE LYMWDSIDQK WTRHYCAIAD 500
    AKLSFSDDIE QTVEEDPVQD TPPTELHFGE KWFHKKVESR TSAEKLLQEY 550
    CAETGAKDGT FLVRESETFP NDYTLSFWRS GRVQHCRIRS TMEGGVMKYY 600
    LTDNLTFNSI YALIQHYREA HLRCAEFELR LTDPVPNPNP HESKPWYYDR 650
    LSRGEAEDML MRIPRDGAFL IRKREGTDSY AITFRARGKV KHCRINRDGR 700
    HFVLGTSAYF ESLVELVSYY EKHALYRKMR LRYPVTPELL ERYNMERDIN 750
    SLYDVSRMYV DPSEINPSMP QRTVKALYDY KAKRSDELTF CRGALIHNVS 800
    KEPGGWWKGD YGTRIQQYFP SNYVEDISAG DAEEMEKQII EDNPLGSLCR 850
    GILDLNTYNV VKAPQGKNQK AFVFILEPKK QGDPPVEFAT DRVEELFEWF 900
    QSIREITWKI DTKENNMKYW ERNQSIAIEL SDLVVYCKPT SKTKDHLENP 950
    DFREIRSFVE TKADSIVRQK PVDLLRYNQK GLTRVYPKGQ RVDSSNYDPF 1000
    RLWLCGSQMV ALNFQTPDKY MQMNHALFSL NGRTGYVLQP ESMRSEKYDP 1050
    MPPESQRKIL MTLTVKVLGA RHLPKLGRSI ACPFVEVEIC GAEYDSNKFK 1100
    TTVVNDNGLS PVWAPTQEKV TFEIYDPNLA FLRFLVYEED MFSDPNFLAH 1150
    ATYPIKGIKS GFRSVPLKNG YSEDIELASL LVFCEMRPVL ESEEELYSSC 1200
    RQLRRRQEEL NNQLFLYDTH QNLRGANRDA LVKEFNVNEN QLRLYQEKCN 1250
    RRLREKRVSN SRFYS 1265
    Length:1,265
    Mass (Da):147,735
    Last modified:March 1, 1992 - v1
    Checksum:i2C34910D43572723
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05155 mRNA. Translation: AAA41896.1.
    PIRiA34163.
    RefSeqiNP_058864.1. NM_017168.1.
    UniGeneiRn.9751.

    Genome annotation databases

    GeneIDi29337.
    KEGGirno:29337.
    UCSCiRGD:3348. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05155 mRNA. Translation: AAA41896.1 .
    PIRi A34163.
    RefSeqi NP_058864.1. NM_017168.1.
    UniGenei Rn.9751.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2EOB NMR - A 633-743 [» ]
    ProteinModelPortali P24135.
    SMRi P24135. Positions 520-744, 772-829.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000018678.

    Chemistry

    ChEMBLi CHEMBL5230.

    PTM databases

    PhosphoSitei P24135.

    Proteomic databases

    PaxDbi P24135.
    PRIDEi P24135.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 29337.
    KEGGi rno:29337.
    UCSCi RGD:3348. rat.

    Organism-specific databases

    CTDi 5336.
    RGDi 3348. Plcg2.

    Phylogenomic databases

    eggNOGi NOG268751.
    HOGENOMi HOG000230864.
    HOVERGENi HBG053611.
    InParanoidi P24135.
    KOi K05859.
    PhylomeDBi P24135.

    Enzyme and pathway databases

    Reactomei REACT_203734. FCERI mediated Ca+2 mobilization.

    Miscellaneous databases

    EvolutionaryTracei P24135.
    NextBioi 608820.
    PROi P24135.

    Gene expression databases

    Genevestigatori P24135.

    Family and domain databases

    Gene3Di 2.30.29.30. 3 hits.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    3.30.505.10. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR001192. PI-PLC_fam.
    IPR016279. PLC-gamma.
    IPR028381. PLC-gamma2.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF25. PTHR10336:SF25. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    PF00017. SH2. 2 hits.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000952. PLC-gamma. 1 hit.
    PRINTSi PR00390. PHPHLIPASEC.
    PR00401. SH2DOMAIN.
    SMARTi SM00239. C2. 1 hit.
    SM00233. PH. 2 hits.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    SM00252. SH2. 2 hits.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF50044. SSF50044. 1 hit.
    SSF51695. SSF51695. 2 hits.
    SSF55550. SSF55550. 2 hits.
    PROSITEi PS50004. C2. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    PS50001. SH2. 2 hits.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A second type of rat phosphoinositide-specific phospholipase C containing a src-related sequence not essential for phosphoinositide-hydrolyzing activity."
      Emori Y., Homma Y., Sorimachi H., Kawasaki H., Nakanishi O., Suzuki K., Takenawa T.
      J. Biol. Chem. 264:21885-21890(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Muscle.
    2. "Identification of the SH2 domain binding protein of Bruton's tyrosine kinase as BLNK--functional significance of Btk-SH2 domain in B-cell antigen receptor-coupled calcium signaling."
      Hashimoto S., Iwamatsu A., Ishiai M., Okawa K., Yamadori T., Matsushita M., Baba Y., Kishimoto T., Kurosaki T., Tsukada S.
      Blood 94:2357-2364(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT TYR-1197.
    3. "Solution structure of the second SH2 domain from rat PLC gamma-2."
      RIKEN structural genomics initiative (RSGI)
      Submitted (APR-2008) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 633-744.

    Entry informationi

    Entry nameiPLCG2_RAT
    AccessioniPrimary (citable) accession number: P24135
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: March 1, 1992
    Last modified: October 1, 2014
    This is version 137 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3