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P24135

- PLCG2_RAT

UniProt

P24135 - PLCG2_RAT

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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2

Gene

Plcg2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Calcium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei327 – 3271PROSITE-ProRule annotation
Active sitei372 – 3721PROSITE-ProRule annotation

GO - Molecular functioni

  1. phosphatidylinositol phospholipase C activity Source: RGD
  2. signal transducer activity Source: UniProtKB-KW

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. phosphatidylinositol biosynthetic process Source: UniProtKB
  3. phospholipid catabolic process Source: InterPro
  4. positive regulation of cytosolic calcium ion concentration Source: RGD
  5. release of sequestered calcium ion into cytosol Source: UniProtKB
  6. response to ATP Source: RGD
  7. response to magnesium ion Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_203734. FCERI mediated Ca+2 mobilization.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-gamma-2
Phospholipase C-IV
Short name:
PLC-IV
Phospholipase C-gamma-2
Short name:
PLC-gamma-2
Gene namesi
Name:Plcg2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3348. Plcg2.

Subcellular locationi

GO - Cellular componenti

  1. membrane Source: RGD
  2. plasma membrane Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 126512651-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2PRO_0000088502Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei753 – 7531Phosphotyrosine; by BTKBy similarity
Modified residuei759 – 7591Phosphotyrosine; by BTKBy similarity
Modified residuei1197 – 11971Phosphotyrosine; by BTK1 Publication
Modified residuei1217 – 12171PhosphotyrosineBy similarity
Modified residuei1245 – 12451PhosphotyrosineBy similarity

Post-translational modificationi

Phosphorylated on tyrosine residues by CSF1R (By similarity). Phosphorylated on tyrosine residues by BTK and SYK; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Phosphorylation leads to increased phospholipase activity (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP24135.
PRIDEiP24135.

PTM databases

PhosphoSiteiP24135.

Expressioni

Gene expression databases

GenevestigatoriP24135.

Interactioni

Subunit structurei

Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018678.

Structurei

Secondary structure

1
1265
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni641 – 6433
Beta strandi647 – 6504
Helixi653 – 66210
Beta strandi665 – 6728
Beta strandi680 – 6867
Beta strandi689 – 70012
Beta strandi702 – 7076
Beta strandi710 – 7123
Helixi713 – 7208
Beta strandi725 – 7284
Helixi737 – 7426

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2EOBNMR-A633-743[»]
ProteinModelPortaliP24135.
SMRiP24135. Positions 520-744, 772-829.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24135.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 131112PHPROSITE-ProRule annotationAdd
BLAST
Domaini312 – 456145PI-PLC X-boxPROSITE-ProRule annotationAdd
BLAST
Domaini532 – 635104SH2 1PROSITE-ProRule annotationAdd
BLAST
Domaini646 – 73590SH2 2PROSITE-ProRule annotationAdd
BLAST
Domaini769 – 82961SH3PROSITE-ProRule annotationAdd
BLAST
Domaini930 – 1044115PI-PLC Y-boxPROSITE-ProRule annotationAdd
BLAST
Domaini1059 – 115294C2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation
Contains 2 SH2 domains.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiNOG268751.
HOGENOMiHOG000230864.
HOVERGENiHBG053611.
InParanoidiP24135.
KOiK05859.
PhylomeDBiP24135.

Family and domain databases

Gene3Di2.30.29.30. 3 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
3.30.505.10. 2 hits.
InterProiIPR000008. C2_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR016279. PLC-gamma.
IPR028381. PLC-gamma2.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF25. PTHR10336:SF25. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF00017. SH2. 2 hits.
PF00018. SH3_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000952. PLC-gamma. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
PR00401. SH2DOMAIN.
SMARTiSM00239. C2. 1 hit.
SM00233. PH. 2 hits.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
SM00252. SH2. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF51695. SSF51695. 2 hits.
SSF55550. SSF55550. 2 hits.
PROSITEiPS50004. C2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
PS50001. SH2. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24135 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTMVNVDTL PEYEKSQIKR ALELGTVMTV FSARKSTPER RTVQMIMETR
60 70 80 90 100
QVAWSKTADK IEGFLDIMEI KEIRPGKNSK DFERAKAVRH KADCCFTIFY
110 120 130 140 150
GTQFVLSTLS LATDSKEDAV KWLSGLKILH QEAMNASTPT MIESWLRKQI
160 170 180 190 200
YSVDQTRRNS ISLRELKTIL PLVNFKVSGI KFLKDKLVEI GAQKDELSFE
210 220 230 240 250
QFHLFYKKLM FEQQKSILDE FKKDSSVFIL GNTDRPDASA VYLQDFQRFL
260 270 280 290 300
LHEQQELWAQ DLNKVRERMT KFIDDTMRET AEPFLFVDEF LTYLFSRENS
310 320 330 340 350
IWDEKYDAVD MQDMNNPLSH YWISSSHNTY LTGDQLRSES STEAYIRCLR
360 370 380 390 400
AGCRCIELDC WDGPDGKPII YHGWTRTTKI KFDDVVQAIR DHAFVTSSFP
410 420 430 440 450
VILSIEEHCS VEQQRHMAKV FKEVLGDLLL TKPTEASADQ LPSPSQLREK
460 470 480 490 500
IIIKHKKLGP RGDVDVNVED KKDEHKTQGE LYMWDSIDQK WTRHYCAIAD
510 520 530 540 550
AKLSFSDDIE QTVEEDPVQD TPPTELHFGE KWFHKKVESR TSAEKLLQEY
560 570 580 590 600
CAETGAKDGT FLVRESETFP NDYTLSFWRS GRVQHCRIRS TMEGGVMKYY
610 620 630 640 650
LTDNLTFNSI YALIQHYREA HLRCAEFELR LTDPVPNPNP HESKPWYYDR
660 670 680 690 700
LSRGEAEDML MRIPRDGAFL IRKREGTDSY AITFRARGKV KHCRINRDGR
710 720 730 740 750
HFVLGTSAYF ESLVELVSYY EKHALYRKMR LRYPVTPELL ERYNMERDIN
760 770 780 790 800
SLYDVSRMYV DPSEINPSMP QRTVKALYDY KAKRSDELTF CRGALIHNVS
810 820 830 840 850
KEPGGWWKGD YGTRIQQYFP SNYVEDISAG DAEEMEKQII EDNPLGSLCR
860 870 880 890 900
GILDLNTYNV VKAPQGKNQK AFVFILEPKK QGDPPVEFAT DRVEELFEWF
910 920 930 940 950
QSIREITWKI DTKENNMKYW ERNQSIAIEL SDLVVYCKPT SKTKDHLENP
960 970 980 990 1000
DFREIRSFVE TKADSIVRQK PVDLLRYNQK GLTRVYPKGQ RVDSSNYDPF
1010 1020 1030 1040 1050
RLWLCGSQMV ALNFQTPDKY MQMNHALFSL NGRTGYVLQP ESMRSEKYDP
1060 1070 1080 1090 1100
MPPESQRKIL MTLTVKVLGA RHLPKLGRSI ACPFVEVEIC GAEYDSNKFK
1110 1120 1130 1140 1150
TTVVNDNGLS PVWAPTQEKV TFEIYDPNLA FLRFLVYEED MFSDPNFLAH
1160 1170 1180 1190 1200
ATYPIKGIKS GFRSVPLKNG YSEDIELASL LVFCEMRPVL ESEEELYSSC
1210 1220 1230 1240 1250
RQLRRRQEEL NNQLFLYDTH QNLRGANRDA LVKEFNVNEN QLRLYQEKCN
1260
RRLREKRVSN SRFYS
Length:1,265
Mass (Da):147,735
Last modified:March 1, 1992 - v1
Checksum:i2C34910D43572723
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05155 mRNA. Translation: AAA41896.1.
PIRiA34163.
RefSeqiNP_058864.1. NM_017168.1.
UniGeneiRn.9751.

Genome annotation databases

GeneIDi29337.
KEGGirno:29337.
UCSCiRGD:3348. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05155 mRNA. Translation: AAA41896.1 .
PIRi A34163.
RefSeqi NP_058864.1. NM_017168.1.
UniGenei Rn.9751.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2EOB NMR - A 633-743 [» ]
ProteinModelPortali P24135.
SMRi P24135. Positions 520-744, 772-829.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000018678.

Chemistry

ChEMBLi CHEMBL5230.

PTM databases

PhosphoSitei P24135.

Proteomic databases

PaxDbi P24135.
PRIDEi P24135.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 29337.
KEGGi rno:29337.
UCSCi RGD:3348. rat.

Organism-specific databases

CTDi 5336.
RGDi 3348. Plcg2.

Phylogenomic databases

eggNOGi NOG268751.
HOGENOMi HOG000230864.
HOVERGENi HBG053611.
InParanoidi P24135.
KOi K05859.
PhylomeDBi P24135.

Enzyme and pathway databases

Reactomei REACT_203734. FCERI mediated Ca+2 mobilization.

Miscellaneous databases

EvolutionaryTracei P24135.
NextBioi 608820.
PROi P24135.

Gene expression databases

Genevestigatori P24135.

Family and domain databases

Gene3Di 2.30.29.30. 3 hits.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
3.30.505.10. 2 hits.
InterProi IPR000008. C2_dom.
IPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR001192. PI-PLC_fam.
IPR016279. PLC-gamma.
IPR028381. PLC-gamma2.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLipase_C_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
IPR000980. SH2.
IPR001452. SH3_domain.
[Graphical view ]
PANTHERi PTHR10336. PTHR10336. 1 hit.
PTHR10336:SF25. PTHR10336:SF25. 1 hit.
Pfami PF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF00017. SH2. 2 hits.
PF00018. SH3_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000952. PLC-gamma. 1 hit.
PRINTSi PR00390. PHPHLIPASEC.
PR00401. SH2DOMAIN.
SMARTi SM00239. C2. 1 hit.
SM00233. PH. 2 hits.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
SM00252. SH2. 2 hits.
SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF49562. SSF49562. 1 hit.
SSF50044. SSF50044. 1 hit.
SSF51695. SSF51695. 2 hits.
SSF55550. SSF55550. 2 hits.
PROSITEi PS50004. C2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
PS50001. SH2. 2 hits.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A second type of rat phosphoinositide-specific phospholipase C containing a src-related sequence not essential for phosphoinositide-hydrolyzing activity."
    Emori Y., Homma Y., Sorimachi H., Kawasaki H., Nakanishi O., Suzuki K., Takenawa T.
    J. Biol. Chem. 264:21885-21890(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Muscle.
  2. "Identification of the SH2 domain binding protein of Bruton's tyrosine kinase as BLNK--functional significance of Btk-SH2 domain in B-cell antigen receptor-coupled calcium signaling."
    Hashimoto S., Iwamatsu A., Ishiai M., Okawa K., Yamadori T., Matsushita M., Baba Y., Kishimoto T., Kurosaki T., Tsukada S.
    Blood 94:2357-2364(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT TYR-1197.
  3. "Solution structure of the second SH2 domain from rat PLC gamma-2."
    RIKEN structural genomics initiative (RSGI)
    Submitted (APR-2008) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 633-744.

Entry informationi

Entry nameiPLCG2_RAT
AccessioniPrimary (citable) accession number: P24135
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: October 29, 2014
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3