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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2

Gene

Plcg2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei327PROSITE-ProRule annotation1
Active sitei372PROSITE-ProRule annotation1

GO - Molecular functioni

  • phosphatidylinositol phospholipase C activity Source: RGD

GO - Biological processi

  • intracellular signal transduction Source: InterPro
  • phosphatidylinositol biosynthetic process Source: UniProtKB
  • phospholipid catabolic process Source: InterPro
  • positive regulation of cytosolic calcium ion concentration Source: RGD
  • release of sequestered calcium ion into cytosol Source: UniProtKB
  • response to ATP Source: RGD
  • response to magnesium ion Source: RGD

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism
LigandCalcium

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-gamma-2
Phospholipase C-IV
Short name:
PLC-IV
Phospholipase C-gamma-2
Short name:
PLC-gamma-2
Gene namesi
Name:Plcg2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3348 Plcg2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5230

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000885021 – 12651-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2Add BLAST1265

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei753Phosphotyrosine; by BTKBy similarity1
Modified residuei759Phosphotyrosine; by BTKBy similarity1
Modified residuei1197Phosphotyrosine; by BTK1 Publication1
Modified residuei1217PhosphotyrosineBy similarity1
Modified residuei1245PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues by CSF1R (By similarity). Phosphorylated on tyrosine residues by BTK and SYK; upon ligand-induced activation of a variety of growth factor receptors and immune system receptors. Phosphorylation leads to increased phospholipase activity (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP24135
PRIDEiP24135

PTM databases

iPTMnetiP24135
PhosphoSitePlusiP24135

Interactioni

Subunit structurei

Interacts (via SH2 domain) with CSF1R (tyrosine phosphorylated).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000018678

Structurei

Secondary structure

11265
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni641 – 643Combined sources3
Beta strandi647 – 650Combined sources4
Helixi653 – 662Combined sources10
Beta strandi665 – 672Combined sources8
Beta strandi680 – 686Combined sources7
Beta strandi689 – 700Combined sources12
Beta strandi702 – 707Combined sources6
Beta strandi710 – 712Combined sources3
Helixi713 – 720Combined sources8
Beta strandi725 – 728Combined sources4
Helixi737 – 742Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EOBNMR-A633-743[»]
ProteinModelPortaliP24135
SMRiP24135
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24135

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 131PHPROSITE-ProRule annotationAdd BLAST112
Domaini312 – 456PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST145
Domaini532 – 635SH2 1PROSITE-ProRule annotationAdd BLAST104
Domaini646 – 735SH2 2PROSITE-ProRule annotationAdd BLAST90
Domaini769 – 829SH3PROSITE-ProRule annotationAdd BLAST61
Domaini930 – 1044PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST115
Domaini1059 – 1152C2PROSITE-ProRule annotationAdd BLAST94

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG1264 Eukaryota
ENOG410XPXE LUCA
HOGENOMiHOG000230864
HOVERGENiHBG053611
InParanoidiP24135
KOiK05859
PhylomeDBiP24135

Family and domain databases

CDDicd09932 SH2_C-SH2_PLC_gamma_like, 1 hit
cd10341 SH2_N-SH2_PLC_gamma_like, 1 hit
cd11969 SH3_PLCgamma2, 1 hit
Gene3Di2.30.29.30, 2 hits
2.60.40.150, 1 hit
3.20.20.190, 2 hits
3.30.505.10, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011992 EF-hand-dom_pair
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR001192 PI-PLC_fam
IPR016279 PLC-gamma
IPR028381 PLC-gamma2
IPR035023 PLC-gamma_C-SH2
IPR035024 PLC-gamma_N-SH2
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR035723 PLCgamma2_SH3
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR10336 PTHR10336, 1 hit
PTHR10336:SF25 PTHR10336:SF25, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PF00017 SH2, 2 hits
PF00018 SH3_1, 1 hit
PIRSFiPIRSF000952 PLC-gamma, 1 hit
PRINTSiPR00390 PHPHLIPASEC
PR00401 SH2DOMAIN
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00233 PH, 2 hits
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SM00252 SH2, 2 hits
SM00326 SH3, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF50044 SSF50044, 1 hit
SSF51695 SSF51695, 2 hits
SSF55550 SSF55550, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50003 PH_DOMAIN, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit
PS50001 SH2, 2 hits
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

P24135-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTMVNVDTL PEYEKSQIKR ALELGTVMTV FSARKSTPER RTVQMIMETR
60 70 80 90 100
QVAWSKTADK IEGFLDIMEI KEIRPGKNSK DFERAKAVRH KADCCFTIFY
110 120 130 140 150
GTQFVLSTLS LATDSKEDAV KWLSGLKILH QEAMNASTPT MIESWLRKQI
160 170 180 190 200
YSVDQTRRNS ISLRELKTIL PLVNFKVSGI KFLKDKLVEI GAQKDELSFE
210 220 230 240 250
QFHLFYKKLM FEQQKSILDE FKKDSSVFIL GNTDRPDASA VYLQDFQRFL
260 270 280 290 300
LHEQQELWAQ DLNKVRERMT KFIDDTMRET AEPFLFVDEF LTYLFSRENS
310 320 330 340 350
IWDEKYDAVD MQDMNNPLSH YWISSSHNTY LTGDQLRSES STEAYIRCLR
360 370 380 390 400
AGCRCIELDC WDGPDGKPII YHGWTRTTKI KFDDVVQAIR DHAFVTSSFP
410 420 430 440 450
VILSIEEHCS VEQQRHMAKV FKEVLGDLLL TKPTEASADQ LPSPSQLREK
460 470 480 490 500
IIIKHKKLGP RGDVDVNVED KKDEHKTQGE LYMWDSIDQK WTRHYCAIAD
510 520 530 540 550
AKLSFSDDIE QTVEEDPVQD TPPTELHFGE KWFHKKVESR TSAEKLLQEY
560 570 580 590 600
CAETGAKDGT FLVRESETFP NDYTLSFWRS GRVQHCRIRS TMEGGVMKYY
610 620 630 640 650
LTDNLTFNSI YALIQHYREA HLRCAEFELR LTDPVPNPNP HESKPWYYDR
660 670 680 690 700
LSRGEAEDML MRIPRDGAFL IRKREGTDSY AITFRARGKV KHCRINRDGR
710 720 730 740 750
HFVLGTSAYF ESLVELVSYY EKHALYRKMR LRYPVTPELL ERYNMERDIN
760 770 780 790 800
SLYDVSRMYV DPSEINPSMP QRTVKALYDY KAKRSDELTF CRGALIHNVS
810 820 830 840 850
KEPGGWWKGD YGTRIQQYFP SNYVEDISAG DAEEMEKQII EDNPLGSLCR
860 870 880 890 900
GILDLNTYNV VKAPQGKNQK AFVFILEPKK QGDPPVEFAT DRVEELFEWF
910 920 930 940 950
QSIREITWKI DTKENNMKYW ERNQSIAIEL SDLVVYCKPT SKTKDHLENP
960 970 980 990 1000
DFREIRSFVE TKADSIVRQK PVDLLRYNQK GLTRVYPKGQ RVDSSNYDPF
1010 1020 1030 1040 1050
RLWLCGSQMV ALNFQTPDKY MQMNHALFSL NGRTGYVLQP ESMRSEKYDP
1060 1070 1080 1090 1100
MPPESQRKIL MTLTVKVLGA RHLPKLGRSI ACPFVEVEIC GAEYDSNKFK
1110 1120 1130 1140 1150
TTVVNDNGLS PVWAPTQEKV TFEIYDPNLA FLRFLVYEED MFSDPNFLAH
1160 1170 1180 1190 1200
ATYPIKGIKS GFRSVPLKNG YSEDIELASL LVFCEMRPVL ESEEELYSSC
1210 1220 1230 1240 1250
RQLRRRQEEL NNQLFLYDTH QNLRGANRDA LVKEFNVNEN QLRLYQEKCN
1260
RRLREKRVSN SRFYS
Length:1,265
Mass (Da):147,735
Last modified:March 1, 1992 - v1
Checksum:i2C34910D43572723
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05155 mRNA Translation: AAA41896.1
PIRiA34163
RefSeqiNP_058864.1, NM_017168.1
UniGeneiRn.9751

Genome annotation databases

GeneIDi29337
KEGGirno:29337
UCSCiRGD:3348 rat

Similar proteinsi

Entry informationi

Entry nameiPLCG2_RAT
AccessioniPrimary (citable) accession number: P24135
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: June 20, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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