Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P24131

- BGAL_CLOAT

UniProt

P24131 - BGAL_CLOAT

Protein

Beta-galactosidase

Gene

cbgA

Organism
Clostridium acetobutylicum
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei459 – 4591Proton donorBy similarity
    Active sitei503 – 5031NucleophileBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidase (EC:3.2.1.23)
    Short name:
    Beta-gal
    Alternative name(s):
    Lactase
    Gene namesi
    Name:cbgA
    OrganismiClostridium acetobutylicum
    Taxonomic identifieri1488 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 897897Beta-galactosidasePRO_0000057660Add
    BLAST

    Expressioni

    Inductioni

    Late in the ABE (acetone, butanol, and ethanol) fermentation and subject to glucose repression.

    Structurei

    3D structure databases

    ProteinModelPortaliP24131.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.Curated

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P24131-1 [UniParc]FASTAAdd to Basket

    « Hide

    MINNKPSLDW LENPEIFRVN RIDAHSDTWF YEKFEDVKLE DTMPLKQNLN    50
    GKWRFSYSEN SSLRIKEFYK DEFDVSWIDY IEVPGHIQLQ GYDKCQYINT 100
    MYPWEGHDEL RPPHISKTYN PVGSYVTFFE VKDELKNKQT FISFQGVETA 150
    FYVWVNGEFV GYSEDTFTPS EFDITDYLRE GENKLAVEVY KRSSASWIED 200
    QDFWRFSGIF RDVYLYAVPE THVNDIFIKT DLYDDFKNAK LNAELKMIGN 250
    SETTVETYLE DKEGNKIAIS EKIPFSDELT LYLDAQNINL WSAEEPNLYT 300
    LYILVNKKDG NLIEVVTQKI GFRHFEMKDK IMCLKWKRII FKGVNRHEFS 350
    ARRGRSITKE DMLWDIKFLK QHNINAVRTS HYPNQSLWYR LCDEYGIYLI 400
    DETNLESHGS WQKMGQIEPS WNVPGSLPQW QAAVLDRASS MVERDKNHPS 450
    VLIWSCGNES YAGEDIYQMS KYFRKKDPSR LVHYEGVTRC REFMTRRHES 500
    RMYAKAAEIE EYLNDNPKKP YISCEYMHSM GNSTGGMMKY TELEDKYLMY 550
    QGGFIWDYGD QALYRKLPDG KEVLAYGGDF TDRPTDYNFS GNGLIYADRT 600
    ISPKAQEVKY LYQNVKLEPD EKGVTIKNQN LFVNTDKYDL YYIVERDGKL 650
    IKDGYLNVSV APDEEKYIEL PIGNYNFPEE IVLTTSLRLA QATLWAEKGY 700
    EIAFGQKVIK EKSDMNNHNS ESKMKIIHGD VNIGVHGKDF KAIFSKQEGG 750
    IVSLRYNNKE FITRTPKTFY WRATTDNDRG NRHEFRCSQW LAATMGQKYV 800
    DFSVEEFDEK ITLYYTYQLP TVPSTNVKIT YEVSGEGIIK VNVKYKGVSG 850
    LPELPVLGMD FKLLAEFNSF SWYGMGPEEN YIDRCEGAKL GIYESTQ 897
    Length:897
    Mass (Da):105,020
    Last modified:February 1, 1996 - v2
    Checksum:iE13DB8674992A32F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35107 Genomic DNA. Translation: AAA23216.1.
    PIRiA39405.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35107 Genomic DNA. Translation: AAA23216.1 .
    PIRi A39405.

    3D structure databases

    ProteinModelPortali P24131.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression and nucleotide sequence of the Clostridium acetobutylicum beta-galactosidase gene cloned in Escherichia coli."
      Hancock K.R., Rockman E., Young C.A., Pearce L., Maddox I.S., Scott D.B.
      J. Bacteriol. 173:3084-3095(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DSM 1732 / NCIB 2951.

    Entry informationi

    Entry nameiBGAL_CLOAT
    AccessioniPrimary (citable) accession number: P24131
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 1992
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 75 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3