P24131 (BGAL_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase
Short name=Beta-gal
EC=3.2.1.23

Alternative name(s):
Lactase

Gene names

Name:

cbgA

Organism

Clostridium acetobutylicum

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	897 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Induction	Late in the ABE (acetone, butanol, and ethanol) fermentation and subject to glucose repression.
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	beta-galactosidase complex Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 897	897	Beta-galactosidase		PRO_0000057660
Sites
	Active site	459	1	Proton donor By similarity
	Active site	503	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P24131 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: E13DB8674992A32F	FASTA	897	105,020
	10 20 30 40 50 60 MINNKPSLDW LENPEIFRVN RIDAHSDTWF YEKFEDVKLE DTMPLKQNLN GKWRFSYSEN 70 80 90 100 110 120 SSLRIKEFYK DEFDVSWIDY IEVPGHIQLQ GYDKCQYINT MYPWEGHDEL RPPHISKTYN 130 140 150 160 170 180 PVGSYVTFFE VKDELKNKQT FISFQGVETA FYVWVNGEFV GYSEDTFTPS EFDITDYLRE 190 200 210 220 230 240 GENKLAVEVY KRSSASWIED QDFWRFSGIF RDVYLYAVPE THVNDIFIKT DLYDDFKNAK 250 260 270 280 290 300 LNAELKMIGN SETTVETYLE DKEGNKIAIS EKIPFSDELT LYLDAQNINL WSAEEPNLYT 310 320 330 340 350 360 LYILVNKKDG NLIEVVTQKI GFRHFEMKDK IMCLKWKRII FKGVNRHEFS ARRGRSITKE 370 380 390 400 410 420 DMLWDIKFLK QHNINAVRTS HYPNQSLWYR LCDEYGIYLI DETNLESHGS WQKMGQIEPS 430 440 450 460 470 480 WNVPGSLPQW QAAVLDRASS MVERDKNHPS VLIWSCGNES YAGEDIYQMS KYFRKKDPSR 490 500 510 520 530 540 LVHYEGVTRC REFMTRRHES RMYAKAAEIE EYLNDNPKKP YISCEYMHSM GNSTGGMMKY 550 560 570 580 590 600 TELEDKYLMY QGGFIWDYGD QALYRKLPDG KEVLAYGGDF TDRPTDYNFS GNGLIYADRT 610 620 630 640 650 660 ISPKAQEVKY LYQNVKLEPD EKGVTIKNQN LFVNTDKYDL YYIVERDGKL IKDGYLNVSV 670 680 690 700 710 720 APDEEKYIEL PIGNYNFPEE IVLTTSLRLA QATLWAEKGY EIAFGQKVIK EKSDMNNHNS 730 740 750 760 770 780 ESKMKIIHGD VNIGVHGKDF KAIFSKQEGG IVSLRYNNKE FITRTPKTFY WRATTDNDRG 790 800 810 820 830 840 NRHEFRCSQW LAATMGQKYV DFSVEEFDEK ITLYYTYQLP TVPSTNVKIT YEVSGEGIIK 850 860 870 880 890 VNVKYKGVSG LPELPVLGMD FKLLAEFNSF SWYGMGPEEN YIDRCEGAKL GIYESTQ

References

[1]	"Expression and nucleotide sequence of the Clostridium acetobutylicum beta-galactosidase gene cloned in Escherichia coli." Hancock K.R., Rockman E., Young C.A., Pearce L., Maddox I.S., Scott D.B. J. Bacteriol. 173:3084-3095(1991) [PubMed: 1850729] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 1732 / NCIB 2951.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M35107 Genomic DNA. Translation: AAA23216.1.
PIR	A39405.
3D structure databases
ProteinModelPortal	P24131.
ModBase	Search...
Protein family/group databases
CAZy	GH2. Glycoside Hydrolase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004199. B-gal_small/dom_5. IPR008979. Galactose-bd-like. IPR011013. Glyco_hydro-type_carb-bd. IPR014718. Glyco_hydro-type_carb-bd_sub. IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR023232. Glyco_hydro_2_AS. IPR023230. Glyco_hydro_2_CS. IPR006102. Glyco_hydro_2_Ig-like. IPR006104. Glyco_hydro_2_N. IPR006103. Glyco_hydro_2_TIM. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 2 hits. G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF02929. Bgal_small_N. 1 hit. PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
PRINTS	PR00132. GLHYDRLASE2.
SMART	SM01038. Bgal_small_N. 1 hit. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF74650. Gal_mut_like. 1 hit. SSF49303. Glyco_hydro_2Ig. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_CLOAB

Accession

Primary (citable) accession number: P24131

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program