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Protein

Beta-galactosidase

Gene

cbgA

Organism
Clostridium acetobutylicum
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei459 – 4591Proton donorBy similarity
Active sitei503 – 5031NucleophileBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:cbgA
OrganismiClostridium acetobutylicum
Taxonomic identifieri1488 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 897897Beta-galactosidasePRO_0000057660Add
BLAST

Expressioni

Inductioni

Late in the ABE (acetone, butanol, and ethanol) fermentation and subject to glucose repression.

Structurei

3D structure databases

ProteinModelPortaliP24131.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24131-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINNKPSLDW LENPEIFRVN RIDAHSDTWF YEKFEDVKLE DTMPLKQNLN
60 70 80 90 100
GKWRFSYSEN SSLRIKEFYK DEFDVSWIDY IEVPGHIQLQ GYDKCQYINT
110 120 130 140 150
MYPWEGHDEL RPPHISKTYN PVGSYVTFFE VKDELKNKQT FISFQGVETA
160 170 180 190 200
FYVWVNGEFV GYSEDTFTPS EFDITDYLRE GENKLAVEVY KRSSASWIED
210 220 230 240 250
QDFWRFSGIF RDVYLYAVPE THVNDIFIKT DLYDDFKNAK LNAELKMIGN
260 270 280 290 300
SETTVETYLE DKEGNKIAIS EKIPFSDELT LYLDAQNINL WSAEEPNLYT
310 320 330 340 350
LYILVNKKDG NLIEVVTQKI GFRHFEMKDK IMCLKWKRII FKGVNRHEFS
360 370 380 390 400
ARRGRSITKE DMLWDIKFLK QHNINAVRTS HYPNQSLWYR LCDEYGIYLI
410 420 430 440 450
DETNLESHGS WQKMGQIEPS WNVPGSLPQW QAAVLDRASS MVERDKNHPS
460 470 480 490 500
VLIWSCGNES YAGEDIYQMS KYFRKKDPSR LVHYEGVTRC REFMTRRHES
510 520 530 540 550
RMYAKAAEIE EYLNDNPKKP YISCEYMHSM GNSTGGMMKY TELEDKYLMY
560 570 580 590 600
QGGFIWDYGD QALYRKLPDG KEVLAYGGDF TDRPTDYNFS GNGLIYADRT
610 620 630 640 650
ISPKAQEVKY LYQNVKLEPD EKGVTIKNQN LFVNTDKYDL YYIVERDGKL
660 670 680 690 700
IKDGYLNVSV APDEEKYIEL PIGNYNFPEE IVLTTSLRLA QATLWAEKGY
710 720 730 740 750
EIAFGQKVIK EKSDMNNHNS ESKMKIIHGD VNIGVHGKDF KAIFSKQEGG
760 770 780 790 800
IVSLRYNNKE FITRTPKTFY WRATTDNDRG NRHEFRCSQW LAATMGQKYV
810 820 830 840 850
DFSVEEFDEK ITLYYTYQLP TVPSTNVKIT YEVSGEGIIK VNVKYKGVSG
860 870 880 890
LPELPVLGMD FKLLAEFNSF SWYGMGPEEN YIDRCEGAKL GIYESTQ
Length:897
Mass (Da):105,020
Last modified:January 31, 1996 - v2
Checksum:iE13DB8674992A32F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35107 Genomic DNA. Translation: AAA23216.1.
PIRiA39405.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35107 Genomic DNA. Translation: AAA23216.1.
PIRiA39405.

3D structure databases

ProteinModelPortaliP24131.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression and nucleotide sequence of the Clostridium acetobutylicum beta-galactosidase gene cloned in Escherichia coli."
    Hancock K.R., Rockman E., Young C.A., Pearce L., Maddox I.S., Scott D.B.
    J. Bacteriol. 173:3084-3095(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 1732 / NCIB 2951.

Entry informationi

Entry nameiBGAL_CLOAT
AccessioniPrimary (citable) accession number: P24131
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 29, 1992
Last sequence update: January 31, 1996
Last modified: September 30, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.