Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P24131 (BGAL_CLOAT) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:cbgA
OrganismClostridium acetobutylicum
Taxonomic identifier1488 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length897 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Induction

Late in the ABE (acetone, butanol, and ethanol) fermentation and subject to glucose repression.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentbeta-galactosidase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

carbohydrate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 897897Beta-galactosidase
PRO_0000057660

Sites

Active site4591Proton donor By similarity
Active site5031Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
P24131 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: E13DB8674992A32F

FASTA897105,020
        10         20         30         40         50         60 
MINNKPSLDW LENPEIFRVN RIDAHSDTWF YEKFEDVKLE DTMPLKQNLN GKWRFSYSEN 

        70         80         90        100        110        120 
SSLRIKEFYK DEFDVSWIDY IEVPGHIQLQ GYDKCQYINT MYPWEGHDEL RPPHISKTYN 

       130        140        150        160        170        180 
PVGSYVTFFE VKDELKNKQT FISFQGVETA FYVWVNGEFV GYSEDTFTPS EFDITDYLRE 

       190        200        210        220        230        240 
GENKLAVEVY KRSSASWIED QDFWRFSGIF RDVYLYAVPE THVNDIFIKT DLYDDFKNAK 

       250        260        270        280        290        300 
LNAELKMIGN SETTVETYLE DKEGNKIAIS EKIPFSDELT LYLDAQNINL WSAEEPNLYT 

       310        320        330        340        350        360 
LYILVNKKDG NLIEVVTQKI GFRHFEMKDK IMCLKWKRII FKGVNRHEFS ARRGRSITKE 

       370        380        390        400        410        420 
DMLWDIKFLK QHNINAVRTS HYPNQSLWYR LCDEYGIYLI DETNLESHGS WQKMGQIEPS 

       430        440        450        460        470        480 
WNVPGSLPQW QAAVLDRASS MVERDKNHPS VLIWSCGNES YAGEDIYQMS KYFRKKDPSR 

       490        500        510        520        530        540 
LVHYEGVTRC REFMTRRHES RMYAKAAEIE EYLNDNPKKP YISCEYMHSM GNSTGGMMKY 

       550        560        570        580        590        600 
TELEDKYLMY QGGFIWDYGD QALYRKLPDG KEVLAYGGDF TDRPTDYNFS GNGLIYADRT 

       610        620        630        640        650        660 
ISPKAQEVKY LYQNVKLEPD EKGVTIKNQN LFVNTDKYDL YYIVERDGKL IKDGYLNVSV 

       670        680        690        700        710        720 
APDEEKYIEL PIGNYNFPEE IVLTTSLRLA QATLWAEKGY EIAFGQKVIK EKSDMNNHNS 

       730        740        750        760        770        780 
ESKMKIIHGD VNIGVHGKDF KAIFSKQEGG IVSLRYNNKE FITRTPKTFY WRATTDNDRG 

       790        800        810        820        830        840 
NRHEFRCSQW LAATMGQKYV DFSVEEFDEK ITLYYTYQLP TVPSTNVKIT YEVSGEGIIK 

       850        860        870        880        890 
VNVKYKGVSG LPELPVLGMD FKLLAEFNSF SWYGMGPEEN YIDRCEGAKL GIYESTQ 

« Hide

References

[1]"Expression and nucleotide sequence of the Clostridium acetobutylicum beta-galactosidase gene cloned in Escherichia coli."
Hancock K.R., Rockman E., Young C.A., Pearce L., Maddox I.S., Scott D.B.
J. Bacteriol. 173:3084-3095(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DSM 1732 / NCIB 2951.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M35107 Genomic DNA. Translation: AAA23216.1.
PIRA39405.

3D structure databases

ProteinModelPortalP24131.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_CLOAT
AccessionPrimary (citable) accession number: P24131
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: February 1, 1996
Last modified: October 16, 2013
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries