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P24118 (CISY_TETTH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Citrate synthase, mitochondrial

EC=2.3.3.16
Alternative name(s):
14 nm filament-forming protein
49 kDa protein
OrganismTetrahymena thermophila
Taxonomic identifier5911 [NCBI]
Taxonomic lineageEukaryotaAlveolataCiliophoraIntramacronucleataOligohymenophoreaHymenostomatidaTetrahymeninaTetrahymenidaeTetrahymena

Protein attributes

Sequence length462 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Structural protein involved in oral morphogenesis and in pronuclear behavior during conjugation. Respiratory enzyme. Ref.1

Catalytic activity

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.

Subunit structure

Homodimer.

Subcellular location

Mitochondrion matrix. Cytoplasm. Cytoplasmcytoskeleton Ref.1.

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Sequence similarities

Belongs to the citrate synthase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2121Mitochondrion Ref.1
Chain22 – 462441Citrate synthase, mitochondrial
PRO_0000005474

Sites

Active site3001 By similarity
Active site3461 By similarity
Active site4011 By similarity

Sequences

Sequence LengthMass (Da)Tools
P24118 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: F4F4EFDAE62243A6

FASTA46252,575
        10         20         30         40         50         60 
MRSINQLLKQ ASLSQKSQYN FSQTNLKKVI AEIIPQKQAE LKEVKEKYGD KVVGQYTVKQ 

        70         80         90        100        110        120 
VIGGMRGMKG LMSDLSRCDP YQGIIFRGYT IPQLKEFLPK ADPKAADQAN QEPLPEGIFW 

       130        140        150        160        170        180 
LLMTGQLPTH AQVDALKHEW QNRGTVNQDC VNFILNLPKD LHSMTMLSMA LLYLQKDSKF 

       190        200        210        220        230        240 
AKLYDEGKIS KKDYWEPFYE DSMDLIAKIP RVAAIIYRHK YRDSKLIDSD SKLDWAGNYA 

       250        260        270        280        290        300 
HMMGFEQHVV KECIRGYLSI HCDHEGGNVS AHTTHLVGSA LSDPYLSYSA GVNGLAGPLH 

       310        320        330        340        350        360 
GLANQEVLKW LLQFIEEKGT KVSDKDIEDY VDHVISSGRV VPGYGHAVLR DTDPRFHHQV 

       370        380        390        400        410        420 
DFSKFHLKDD QMIKLLHQCA DVIPKKLLTY KKIANPYPNV DCHSGVLLYS LGLTEYQYYT 

       430        440        450        460 
VVFAVSRALG CMANLIWSRA FGLPIERPGS ADLKWFHDKY RE 

« Hide

References

[1]"Tetrahymena 14-nm filament-forming protein has citrate synthase activity."
Numata O., Takemasa T., Takagi I., Hirono M., Hirano H., Chiba J., Watanabe Y.
Biochem. Biophys. Res. Commun. 174:1028-1034(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-40, SUBCELLULAR LOCATION, FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D90117 mRNA. Translation: BAA14145.1.
PIRJC5625.

3D structure databases

ProteinModelPortalP24118.
SMRP24118. Positions 24-455.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5911.P24118.

Proteomic databases

PRIDEP24118.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG0372.

Enzyme and pathway databases

UniPathwayUPA00223; UER00717.

Family and domain databases

Gene3D1.10.580.10. 1 hit.
InterProIPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
[Graphical view]
PANTHERPTHR11739. PTHR11739. 1 hit.
PfamPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSPR00143. CITRTSNTHASE.
SUPFAMSSF48256. SSF48256. 1 hit.
PROSITEPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCISY_TETTH
AccessionPrimary (citable) accession number: P24118
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: June 11, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways