Reviewed, UniProtKB/Swiss-Prot P24114 (LUXA_VIBAB)

Names and origin

Protein names

Recommended name:
    Alkanal monooxygenase alpha chain
    EC=1.14.14.3
Alternative name(s):
    Bacterial luciferase alpha chain

Gene names

Name:

luxA

Organism

Vibrio albensis

Taxonomic identifier

140100 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	103 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Light-emitting reaction in luminous bacteria.
Catalytic activity	RCHO + reduced FMN + O2 = RCOOH + FMN + H2O + light.
Subunit structure	Heterodimer of an alpha and a beta chain.
Miscellaneous	The synthesis of this protein is regulated by a complex control mechanism that has been termed autoinduction. In fully induced cells luciferase comprises up to 5% of the soluble protein. Strain ATCC 14547 is luminescent. Strain UM4102 is dimly luminescent. Strain UM4082 is non-luminescent.

Ontologies

Keywords
Biological process	Luminescence
Ligand	FMN Flavoprotein
Molecular function	Monooxygenase Oxidoreductase Photoprotein
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alkanal monooxygenase (FMN-linked) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›103	›103	Alkanal monooxygenase alpha chain		PRO_0000220170
Natural variations
	Natural variant	101	1	Y → F in strain: UM4082.
	Natural variant	101	1	Y → S in strain: UM4102.
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	103	1

Sequences

	Sequence		Length	Mass (Da)
	P24114-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: D0DE5D3EEAB137D2	FASTA	103	12,337
	10 20 30 40 50 60 INTHEKKAQL DLYNEIALEH GHDIQNIDHC LSYITSVDHD SQRAKDICRQ FLAHWYDSYV 70 80 90 100 NATRIFDDSD QTKGYDFNKG QWRDFVLKGH RDTNRRIDYS YEI

References

[1]

"Detection of luciferase gene sequence in nonluminescent Vibrio cholerae by colony hybridization and polymerase chain reaction." Palmer L.M., Colwell R.R. Appl. Environ. Microbiol. 57:1286-1293(1991) [PubMed: 1854193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 14547 / LMG 4406 / NCIMB 41, UM4082 and UM4102.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M60437 Genomic DNA. Translation: AAA27532.1. M60438 Genomic DNA. Translation: AAA27533.1. M60439 Genomic DNA. Translation: AAA27534.1.
PIR	B49775. C49775.
3D structure databases
HSSP	HSSP built from PDB template 1BRL based on UniProtKB P07740.
SMR	P24114. Positions 1-103.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.14.3. 308101.
Family and domain databases
InterPro	IPR018235. Bacterial_luciferase_CS. IPR011251. Luciferase-like. IPR016048. Luciferase-like_sub. IPR002103. Luciferase_bac. [Graphical view]
Gene3D	G3DSA:3.20.20.30. Luciferase_like. 1 hit.
Pfam	PF00296. Bac_luciferase. 1 hit. [Graphical view]
PRINTS	PR00089. LUCIFERASE.
PROSITE	PS00494. BACTERIAL_LUCIFERASE. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUXA_VIBAB

Accession

Primary (citable) accession number: P24114

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)