Reviewed, UniProtKB/Swiss-Prot P24113 (LUXA_PHOPO)

Names and origin

Protein names

Recommended name:
    Alkanal monooxygenase alpha chain
    EC=1.14.14.3
Alternative name(s):
    Bacterial luciferase alpha chain

Gene names

Name:

luxA

Organism

Photobacterium phosphoreum

Taxonomic identifier

659 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Photobacterium

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Light-emitting reaction in luminous bacteria.
Catalytic activity	RCHO + reduced FMN + O2 = RCOOH + FMN + H2O + light.
Subunit structure	Heterodimer of an alpha and a beta chain.

Ontologies

Keywords
Biological process	Luminescence
Ligand	FMN Flavoprotein
Molecular function	Monooxygenase Oxidoreductase Photoprotein
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alkanal monooxygenase (FMN-linked) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Alkanal monooxygenase alpha chain		PRO_0000220169
Experimental info
	Sequence conflict	6	1	I → IC in AAA25627. Ref.2
	Sequence conflict	40 – 41	2	LE → TL in AAA25627. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P24113-1 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: 360EC324E0A438A0	FASTA	346	39,364
	10 20 30 40 50 60 MKFGNIFSYQ PPGESHKEVM DRFVRLGVAS EELNFDTYWL EHHFTEFGLT GNLFVACANL 70 80 90 100 110 120 LGRTTKLNVG TMIVLPTAHP ARQMEDLLLL DQMSKGRFNF GVVRGYHKDF RVFGVTMEDS 130 140 150 160 170 180 RAITEDFHTM IMDGTKTGLH TDGKNIEFPD VNVYPEAYLE KIPTCMTAES ATTTWLAERG 190 200 210 220 230 240 LPMVLSWIIT TSEKKAQMEL YNAVRDSGYS EEYIKNVDHS MTLICSVDED GKKAEDVREF 250 260 270 280 290 300 LGNWYDSYVN ATNIFSESNQ TRGYDYHKGQ KDFVLQGHTN TKRRVDYSND LNPVGTPEKC 310 320 330 340 IEIQRDIDAT GITNITLGFE ANGSEEEIIA SMKRFMQVAP FLKDPK

References

[1]	"Structure and properties of luciferase from Photobacterium phosphoreum." Ferri S.R., Soly R.R., Szittner R.B., Meighen E.A. Biochem. Biophys. Res. Commun. 176:541-548(1991) [PubMed: 2018544] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A lux-specific myristoyl transferase in luminescent bacteria related to eukaryotic serine esterases." Ferri S.R., Meighen E.A. J. Biol. Chem. 266:12852-12857(1991) [PubMed: 2071574] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-41.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M65067 Genomic DNA. Translation: AAA70297.1. M64224 Genomic DNA. Translation: AAA25627.1.
PIR	JH0387.
3D structure databases
HSSP	HSSP built from PDB template 1LUC based on UniProtKB P07740.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.14.3. 348.
Family and domain databases
InterPro	IPR018235. Bacterial_luciferase_CS. IPR011251. Luciferase-like. IPR016048. Luciferase-like_sub. IPR002103. Luciferase_bac. [Graphical view]
Gene3D	G3DSA:3.20.20.30. Luciferase_like. 1 hit.
Pfam	PF00296. Bac_luciferase. 1 hit. [Graphical view]
PRINTS	PR00089. LUCIFERASE.
PROSITE	PS00494. BACTERIAL_LUCIFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUXA_PHOPO

Accession

Primary (citable) accession number: P24113

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)