P24112 (PELD_ERWCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pectin lyase
EC=4.2.2.10

Gene names

Name:

pnl

Organism

Erwinia carotovora

Taxonomic identifier

554 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Pectobacterium

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Eliminative cleavage of (1->4)-alpha-D-galacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.
Induction	By DNA-damaging agents such as nalidixic acid, mitomycin C or UV light.
Miscellaneous	In most stains, pnl production is accompanied by cell lysis and production of a bacteriocin.
Sequence similarities	Belongs to the polysaccharide lyase 1 family.

Ontologies

Keywords
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular function	pectin lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Pectin lyase		PRO_0000212995
Sites
	Active site	202	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	P24112 [UniParc]. Last modified March 1, 1992. Version 1. Checksum: D94769EA15DB4DE1	FASTA	314	33,709
	10 20 30 40 50 60 MAYPTTNLTG LIGFAKAAKV TGGTGGKVVT VNSLADFKSA VSGSAKTIVV LGSSLKTSAL 70 80 90 100 110 120 TKVVFGSNKT IVGSFGGANV LTNIHLRAES NSSNVIFQNL VFKHDVAIKD NDDIQLYLNY 130 140 150 160 170 180 GKGYWVDHCS WPGHTWSDND GSLDKLIYIG EKADYITISN CLFSNHKYGC IFGHPADDNN 190 200 210 220 230 240 SAYNGYPRLT ICHNYYENIQ VRAPGLMRYG YFHVFNNYVN KFQLAFTVAQ NANVISERNV 250 260 270 280 290 300 FGSGAEKKGM VDDKGNGSTF TDNGSSPAAV ASKSPAAKWT ASSNYSYSLM TTAAAQSWVV 310 SNAGAQNSAL KFPS

References

[1]	"Nucleotide sequence of pnl gene from Erwinia carotovora Er." Ohnishi H., Nishida T., Yoshida A., Kamio Y., Izaki K. Biochem. Biophys. Res. Commun. 176:321-327(1991) [PubMed: 2018526] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Er.
[2]	"Cloning and expression of pectin lyase gene from Erwinia carotovora in Escherichia coli." Nishida T., Suzuki T., Ito K., Kamio Y., Izaki K. Biochem. Biophys. Res. Commun. 168:801-808(1990) [PubMed: 2185758] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-18. Strain: Er.
[3]	"Analysis of promoter region of the pectin lyase gene from Erwinia carotovora Er." Ohnishi H., Nikaidou N., Kamio Y., Izaki K. Biosci. Biotechnol. Biochem. 58:432-433(1994) [PubMed: 7764549] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-20.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M65057 Genomic DNA. Translation: AAA24857.1.
PIR	JH0389.
3D structure databases
ProteinModelPortal	P24112.
ModBase	Search...
Protein family/group databases
CAZy	PL1. Polysaccharide Lyase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002022. Amb_allergen. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00544. Pec_lyase_C. 1 hit. [Graphical view]
SMART	SM00656. Amb_all. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PELD_ERWCA

Accession

Primary (citable) accession number: P24112

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 1992
Last sequence update:	March 1, 1992
Last modified:	May 31, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program