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P24101 (PER33_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peroxidase 33
Short name=Atperox P33
EC=1.11.1.7
Alternative name(s):
ATPCa
Neutral peroxidase C
Short name=PERC
Gene names
Name:PER33
Synonyms:P33, PRXCA
Ordered Locus Names:At3g49110
ORF Names:T2J13.50, F2K15.4
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Removal of H2O2, oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.

May be implicated in the systemic acquired resistance response via the salicylic acid signal transduction pathway.

Catalytic activity

2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O.

Cofactor

Binds 1 heme B (iron-protoporphyrin IX) group per subunit By similarity.

Binds 2 calcium ions per subunit By similarity.

Subcellular location

Secreted Probable. Vacuole Probable. Note: Carboxy-terminal extension appears to target the protein to vacuoles.

Tissue specificity

Expressed in roots.

Induction

Transiently induced by ozone treatment. Up-regulated during a continuous drought stress. Early induced by benzothiadiazol, a chemical analog of salicylic acid. Enhanced expression following both compatible or incompatible pathogen attacks. Ref.6 Ref.7 Ref.8

Miscellaneous

There are 73 peroxidase genes in A.thaliana.

Sequence similarities

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Potential
Chain32 – 354323Peroxidase 33
PRO_0000023699

Sites

Active site731Proton acceptor
Metal binding741Calcium 1 By similarity
Metal binding771Calcium 1; via carbonyl oxygen By similarity
Metal binding791Calcium 1; via carbonyl oxygen By similarity
Metal binding811Calcium 1 By similarity
Metal binding831Calcium 1 By similarity
Metal binding2011Iron (heme axial ligand) By similarity
Metal binding2021Calcium 2 By similarity
Metal binding2531Calcium 2 By similarity
Metal binding2561Calcium 2 By similarity
Metal binding2611Calcium 2 By similarity
Binding site1701Substrate; via carbonyl oxygen By similarity
Site691Transition state stabilizer By similarity

Amino acid modifications

Modified residue321Pyrrolidone carboxylic acid By similarity
Glycosylation881N-linked (GlcNAc...) Potential
Glycosylation2171N-linked (GlcNAc...) Potential
Glycosylation2291N-linked (GlcNAc...) Potential
Glycosylation2451N-linked (GlcNAc...) Potential
Glycosylation2861N-linked (GlcNAc...) Potential
Disulfide bond42 ↔ 122 By similarity
Disulfide bond75 ↔ 80 By similarity
Disulfide bond128 ↔ 332 By similarity
Disulfide bond208 ↔ 240 By similarity

Sequences

Sequence LengthMass (Da)Tools
P24101 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 6D2EE3D536111724

FASTA35438,941
        10         20         30         40         50         60 
MQFSSSSITS FTWTVLITVG CLMLCASFSD AQLTPTFYDT SCPTVTNIVR DTIVNELRSD 

        70         80         90        100        110        120 
PRIAGSILRL HFHDCFVNGC DASILLDNTT SFRTEKDALG NANSARGFPV IDRMKAAVER 

       130        140        150        160        170        180 
ACPRTVSCAD MLTIAAQQSV TLAGGPSWKV PLGRRDSLQA FLDLANANLP APFFTLPQLK 

       190        200        210        220        230        240 
ANFKNVGLDR PSDLVALSGA HTFGKNQCRF IMDRLYNFSN TGLPDPTLNT TYLQTLRGQC 

       250        260        270        280        290        300 
PRNGNQSVLV DFDLRTPLVF DNKYYVNLKE QKGLIQSDQE LFSSPNATDT IPLVRAYADG 

       310        320        330        340        350 
TQTFFNAFVE AMNRMGNITP TTGTQGQIRL NCRVVNSNSL LHDVVDIVDF VSSM

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequences of two genomic DNAs encoding peroxidase of Arabidopsis thaliana."
Intapruk C., Higashimura N., Yamamoto K., Okada N., Shinmyo A., Takano M.
Gene 98:237-241(1991) [PubMed: 2016063] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed: 11130713] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Computational analyses and annotations of the Arabidopsis peroxidase gene family."
Oestergaard L., Pedersen A.G., Jespersen H.M., Brunak S., Welinder K.G.
FEBS Lett. 433:98-102(1998) [PubMed: 9738941] [Abstract]
Cited for: CHARACTERIZATION.
Strain: cv. Columbia.
[6]"Ozone-induced expression of stress-related genes in Arabidopsis thaliana."
Sharma Y.K., Davis K.R.
Plant Physiol. 105:1089-1096(1994) [PubMed: 12232267] [Abstract]
Cited for: INDUCTION.
Strain: cv. Columbia.
[7]"The transcriptome of Arabidopsis thaliana during systemic acquired resistance."
Maleck K., Levine A., Eulgem T., Morgan A., Schmid J., Lawton K.A., Dangl J.L., Dietrich R.A.
Nat. Genet. 26:403-410(2000) [PubMed: 11101835] [Abstract]
Cited for: INDUCTION.
Strain: cv. Wassilewskija.
[8]"Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray."
Seki M., Narusaka M., Ishida J., Nanjo T., Fujita M., Oono Y., Kamiya A., Nakajima M., Enju A., Sakurai T., Satou M., Akiyama K., Taji T., Yamaguchi-Shinozaki K., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Plant J. 31:279-292(2002) [PubMed: 12164808] [Abstract]
Cited for: INDUCTION.
Strain: cv. Columbia.
[9]"Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana."
Tognolli M., Penel C., Greppin H., Simon P.
Gene 288:129-138(2002) [PubMed: 12034502] [Abstract]
Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M58380 Genomic DNA. Translation: AAA32849.1.
AL132956 Genomic DNA. No translation available.
AL132967 Genomic DNA. Translation: CAB61999.1.
CP002686 Genomic DNA. Translation: AEE78499.1.
AY049304 mRNA. Translation: AAK83646.1.
AY052673 mRNA. Translation: AAK96577.1.
IPIIPI00531114.
PIRJU0457.
RefSeqNP_190480.1. NM_114770.2.
UniGeneAt.23913.
At.47588.

3D structure databases

ProteinModelPortalP24101.
SMRP24101. Positions 32-337.
ModBaseSearch...

Protein-protein interaction databases

STRINGP24101.

Protein family/group databases

PeroxiBase199. AtPrx33.

Proteomic databases

PRIDEP24101.
ProMEXP24101.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G49110.1; AT3G49110.1; AT3G49110.
GeneID824072.
GenomeReviewsGene locus AT3G49110 in contig BA000014_GR.
KEGGath:AT3G49110.
NMPDRfig|3702.1.peg.16155.

Organism-specific databases

GeneFarm1860. 61.
TAIRAt3g49110.

Phylogenomic databases

GeneTreeEPGT00070000028241.
HOGENOMHBG597790.
InParanoidP24101.
OMAPLVFDNK.
PhylomeDBP24101.
ProtClustDBCLSN2683115.

Gene expression databases

GenevestigatorP24101.
GermOnlineAT3G49110. Arabidopsis thaliana.

Family and domain databases

InterProIPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR000823. Peroxidase_pln.
IPR019794. Peroxidases_AS.
IPR019793. Peroxidases_heam-ligand_BS.
[Graphical view]
KOK00430.
PfamPF00141. peroxidase. 1 hit.
[Graphical view]
PRINTSPR00458. PEROXIDASE.
PR00461. PLPEROXIDASE.
SUPFAMSSF48113. Peroxidase_super. 1 hit.
PROSITEPS00435. PEROXIDASE_1. 1 hit.
PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePER33_ARATH
AccessionPrimary (citable) accession number: P24101
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: December 14, 2011
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents