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Protein

Cyclin-dependent kinase A-1

Gene

CDKA-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the control of the cell cycle. Essential for both G1/S and G2/M (mitosis) phase transitions. Functions in cell morphogenesis as well as cell proliferation. Required for cell division (entry into mitosis) of the generative cell in male gametogenesis.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulationi

CDK kinase activated by CDKF-1. CDK kinase activity inhibited by KRP1/ICK1, KRP2/ICK2, KRP3/ICK6, KRP4/ICK7, KRP5/ICK3, KRP6/ICK4 and KRP7/ICK5. Down-regulated by phosphorylation by WEE1.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATPPROSITE-ProRule annotation1
Active sitei127Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cyclin-dependent protein serine/threonine kinase activity Source: TAIR
  • kinase activity Source: TAIR
  • protein kinase activity Source: TAIR
  • RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

GO - Biological processi

  • asymmetric cell division Source: TAIR
  • cytokinesis by cell plate formation Source: TAIR
  • DNA endoreduplication Source: TAIR
  • embryo development ending in seed dormancy Source: TAIR
  • gametophyte development Source: TAIR
  • mitotic nuclear division Source: UniProtKB-KW
  • pollen development Source: TAIR
  • positive regulation of cell proliferation Source: TAIR
  • regulation of meiotic nuclear division Source: TAIR
  • response to cold Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 399.
ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-113510. E2F mediated regulation of DNA replication.
R-ATH-1538133. G0 and Early G1.
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-68911. G2 Phase.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-68962. Activation of the pre-replicative complex.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-69205. G1/S-Specific Transcription.
R-ATH-69273. Cyclin A/B1 associated events during G2/M transition.
R-ATH-69656. Cyclin A:Cdk2-associated events at S phase entry.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase A-1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDKA;1
Alternative name(s):
Cell division control protein 2 homolog A
Gene namesi
Name:CDKA-1
Synonyms:CDC2, CDC2A
Ordered Locus Names:At3g48750
ORF Names:T21J18.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G48750.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Mainly cytoplasmic. Nuclear distribution increases after binding to ICK1/KRP1.

GO - Cellular componenti

  • cortical microtubule, transverse to long axis Source: TAIR
  • cytoplasm Source: TAIR
  • cytosol Source: TAIR
  • nucleus Source: TAIR
  • plasma membrane Source: TAIR
  • preprophase band Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Plants display lethal male gametophyte.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14T → A: Increased kinase activity; when associated with F-15. 1 Publication1
Mutagenesisi15Y → F: Abolishes phosphorylation by WEE1. Increased kinase activity; when associated with A-14. 2 Publications1
Mutagenesisi146D → N: Decreased kinase activity and disturbed cell cycle. Reduced frequency of cell division during embryo development. 2 Publications1
Mutagenesisi156P → L: Decreased kinase activity and disturbed cell cycle. 1 Publication1
Mutagenesisi161T → D: Strong reduction in kinase activity and ability to bind substrate. Strong reduction in plant growth. Sterile plants. 1 Publication1
Mutagenesisi161T → V: Strong reduction in kinase activity and ability to bind substrate. 1 Publication1
Mutagenesisi166T → I: Decreased kinase activity and disturbed cell cycle. 1 Publication1
Mutagenesisi234 – 236Missing : No change in kinase activity, but disturbed cell cycle. Loss of interaction with CKS1. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000857491 – 294Cyclin-dependent kinase A-1Add BLAST294

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15Phosphotyrosine1 Publication1
Modified residuei161PhosphothreonineCombined sources1 Publication1

Post-translational modificationi

Phosphorylated at Tyr-15 by WEE1. Phosphorylation at Thr-161 is important for the kinase activity and substrate binding. Binding to the anti-phosphatase PAS2 prevents dephosphorylation.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP24100.
PRIDEiP24100.

PTM databases

iPTMnetiP24100.

Expressioni

Tissue specificityi

Expressed in roots, stems, flowers and siliques.1 Publication

Developmental stagei

Expressed throughout the cell cycle. Expressed in actively dividing cells: root and shoot apical meristems, leaf primordia and emerging lateral root meristem. Expressed in light-grown seedlings from 1 up to 7 days after germination with a peak at 2 and 3 days.2 Publications

Inductioni

By nematode infection in roots. Down-regulated by salt stress in root meristem and replication blocking agents (hydroxyurea and aphidicolin).3 Publications

Gene expression databases

GenevisibleiP24100. AT.

Interactioni

Subunit structurei

Interacts with CDT1A, CYCA2-3, CYCD2-1, CYCD3-1, CYCD4-1, CYCD4-2, CYCH1-1, CYCU1-1, CYCU2-1, CYCU2-2, CYCU3-1, CYCU4-1, CYCU4-2, CYCU4-3, CKS1, KRP2/ICK2, KRP3/ICK6, KRP4/ICK7, KRP6/ICK4, KRP7/ICK5, and C-terminal domain of KRP1/ICK1. Interacts with WEE1 and TIF4A-1/EIF4A-1. Interacts with PAS2; when phosphorylated at Tyr-15. Interacts with SMR3, SMR4, SMR5, SMR6, SMR8 and At4g14310.25 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CKS1O232499EBI-371713,EBI-1253127
CYCD2-1P427524EBI-371713,EBI-1253160
CYCD3-1P427535EBI-371713,EBI-1253610
CYCD4-1Q8LGA14EBI-371713,EBI-1253202
CYCU1-1Q9LJ452EBI-371713,EBI-1773749
CYCU4-1O805132EBI-371713,EBI-1773819
CYCU4-3Q9FKF62EBI-371713,EBI-1773829
KRP1Q67Y9310EBI-371713,EBI-1636730
KRP2Q9SCR26EBI-371713,EBI-1636748
KRP3Q9FKB54EBI-371713,EBI-1773302
KRP4Q8GYJ34EBI-371713,EBI-1253225
KRP5Q9LRY03EBI-371713,EBI-1636764
KRP6Q0WNX93EBI-371713,EBI-1253171
KRP7Q94CL93EBI-371713,EBI-1773344
TIF4A-1P413762EBI-371713,EBI-371706

Protein-protein interaction databases

BioGridi9354. 105 interactors.
IntActiP24100. 73 interactors.
STRINGi3702.AT3G48750.1.

Structurei

3D structure databases

ProteinModelPortaliP24100.
SMRiP24100.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 287Protein kinasePROSITE-ProRule annotationAdd BLAST284

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0594. Eukaryota.
ENOG410XPP3. LUCA.
HOGENOMiHOG000233024.
InParanoidiP24100.
KOiK02206.
OMAiISTFPKW.
OrthoDBiEOG09360GJR.
PhylomeDBiP24100.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24100-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQYEKVEKI GEGTYGVVYK ARDKVTNETI ALKKIRLEQE DEGVPSTAIR
60 70 80 90 100
EISLLKEMQH SNIVKLQDVV HSEKRLYLVF EYLDLDLKKH MDSTPDFSKD
110 120 130 140 150
LHMIKTYLYQ ILRGIAYCHS HRVLHRDLKP QNLLIDRRTN SLKLADFGLA
160 170 180 190 200
RAFGIPVRTF THEVVTLWYR APEILLGSHH YSTPVDIWSV GCIFAEMISQ
210 220 230 240 250
KPLFPGDSEI DQLFKIFRIM GTPYEDTWRG VTSLPDYKSA FPKWKPTDLE
260 270 280 290
TFVPNLDPDG VDLLSKMLLM DPTKRINARA ALEHEYFKDL GGMP
Length:294
Mass (Da):34,030
Last modified:March 1, 1992 - v1
Checksum:iB5FAE55FA9EC366E
GO

Sequence cautioni

The sequence AAL91258 differs from that shown. Reason: Frameshift at position 285.Curated
The sequence CAB87903 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59198 mRNA. Translation: AAA32831.1.
S45387 mRNA. Translation: AAB23643.1.
X57839 mRNA. Translation: CAA40971.1.
D10850 Genomic DNA. Translation: BAA01623.1.
AL132963 Genomic DNA. Translation: CAB87903.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78452.1.
AY090353 mRNA. Translation: AAL91258.1. Frameshift.
BT024706 mRNA. Translation: ABD59044.1.
AK226373 mRNA. Translation: BAE98520.1.
AY085153 mRNA. Translation: AAM61706.1.
PIRiS23095.
T49271.
RefSeqiNP_566911.1. NM_114734.4.
UniGeneiAt.24166.

Genome annotation databases

EnsemblPlantsiAT3G48750.1; AT3G48750.1; AT3G48750.
GeneIDi824036.
GrameneiAT3G48750.1; AT3G48750.1; AT3G48750.
KEGGiath:AT3G48750.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59198 mRNA. Translation: AAA32831.1.
S45387 mRNA. Translation: AAB23643.1.
X57839 mRNA. Translation: CAA40971.1.
D10850 Genomic DNA. Translation: BAA01623.1.
AL132963 Genomic DNA. Translation: CAB87903.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78452.1.
AY090353 mRNA. Translation: AAL91258.1. Frameshift.
BT024706 mRNA. Translation: ABD59044.1.
AK226373 mRNA. Translation: BAE98520.1.
AY085153 mRNA. Translation: AAM61706.1.
PIRiS23095.
T49271.
RefSeqiNP_566911.1. NM_114734.4.
UniGeneiAt.24166.

3D structure databases

ProteinModelPortaliP24100.
SMRiP24100.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9354. 105 interactors.
IntActiP24100. 73 interactors.
STRINGi3702.AT3G48750.1.

PTM databases

iPTMnetiP24100.

Proteomic databases

PaxDbiP24100.
PRIDEiP24100.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G48750.1; AT3G48750.1; AT3G48750.
GeneIDi824036.
GrameneiAT3G48750.1; AT3G48750.1; AT3G48750.
KEGGiath:AT3G48750.

Organism-specific databases

TAIRiAT3G48750.

Phylogenomic databases

eggNOGiKOG0594. Eukaryota.
ENOG410XPP3. LUCA.
HOGENOMiHOG000233024.
InParanoidiP24100.
KOiK02206.
OMAiISTFPKW.
OrthoDBiEOG09360GJR.
PhylomeDBiP24100.

Enzyme and pathway databases

BRENDAi2.7.11.22. 399.
ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-113510. E2F mediated regulation of DNA replication.
R-ATH-1538133. G0 and Early G1.
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-68911. G2 Phase.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-68962. Activation of the pre-replicative complex.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-69205. G1/S-Specific Transcription.
R-ATH-69273. Cyclin A/B1 associated events during G2/M transition.
R-ATH-69656. Cyclin A:Cdk2-associated events at S phase entry.

Miscellaneous databases

PROiP24100.

Gene expression databases

GenevisibleiP24100. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCDKA1_ARATH
AccessioniPrimary (citable) accession number: P24100
Secondary accession number(s): Q29Q50, Q8RX68, Q9M307
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 30, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.