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Protein

Cyclin-dependent kinase A-1

Gene

CDKA-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the control of the cell cycle. Essential for both G1/S and G2/M (mitosis) phase transitions. Functions in cell morphogenesis as well as cell proliferation. Required for cell division (entry into mitosis) of the generative cell in male gametogenesis. Required to trigger guard mother cells (GMC) symmetric divisions at the late stage of stomatal development, probably via the regulation of G1 to S transition in the cell cycle. Promotes divisions in the guard cells (GCs) after the guard mother cells (GMC) symmetric division when in the presence of CYCD3-2 (PubMed:24687979).4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme regulationi

CDK kinase activated by CDKF-1. CDK kinase activity inhibited by KRP1/ICK1, KRP2/ICK2, KRP3/ICK6, KRP4/ICK7, KRP5/ICK3, KRP6/ICK4 and KRP7/ICK5. Down-regulated by phosphorylation by WEE1.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATPPROSITE-ProRule annotation1
Active sitei127Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cyclin-dependent protein serine/threonine kinase activity Source: TAIR
  • kinase activity Source: TAIR
  • protein kinase activity Source: TAIR
  • RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

GO - Biological processi

  • asymmetric cell division Source: TAIR
  • cytokinesis by cell plate formation Source: TAIR
  • DNA endoreduplication Source: TAIR
  • DNA repair Source: GO_Central
  • embryo development ending in seed dormancy Source: TAIR
  • gametophyte development Source: TAIR
  • guard mother cell cytokinesis Source: UniProtKB
  • guard mother cell differentiation Source: UniProtKB
  • histone phosphorylation Source: GO_Central
  • microtubule cytoskeleton organization Source: GO_Central
  • mitotic cell cycle phase transition Source: GO_Central
  • pollen development Source: UniProtKB
  • positive regulation of cell proliferation Source: TAIR
  • regulation of cell cycle G1/S phase transition Source: UniProtKB
  • regulation of gene expression Source: GO_Central
  • regulation of meiotic nuclear division Source: TAIR
  • regulation of mitotic cell cycle Source: GO_Central
  • response to cold Source: TAIR
  • symmetric cell division Source: UniProtKB

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division, Mitosis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 399.
ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-1538133. G0 and Early G1.
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-ATH-2299718. Condensation of Prophase Chromosomes.
R-ATH-2500257. Resolution of Sister Chromatid Cohesion.
R-ATH-4419969. Depolymerisation of the Nuclear Lamina.
R-ATH-5693607. Processing of DNA double-strand break ends.
R-ATH-6804114. TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
R-ATH-68911. G2 Phase.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-68962. Activation of the pre-replicative complex.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-69200. Phosphorylation of proteins involved in G1/S transition by active Cyclin E:Cdk2 complexes.
R-ATH-69202. Cyclin E associated events during G1/S transition.
R-ATH-69273. Cyclin A/B1/B2 associated events during G2/M transition.
R-ATH-69478. G2/M DNA replication checkpoint.
R-ATH-69656. Cyclin A:Cdk2-associated events at S phase entry.
R-ATH-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase A-1 (EC:2.7.11.22, EC:2.7.11.23)
Short name:
CDKA;1
Alternative name(s):
Cell division control protein 2 homolog A
Gene namesi
Name:CDKA-1
Synonyms:CDC2, CDC2A
Ordered Locus Names:At3g48750
ORF Names:T21J18.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G48750.
TAIRilocus:2099478. AT3G48750.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Plants display lethal male gametophyte (PubMed:16460514). Impaired stomata formation with arrested guard mother cells (GMC) divisions. Impaired last mitotic division in the male gametophyte, leading to 50 percent of pollen with two gametes. The double mutant flp-1 myb88 displays an enhanced stomatal phenotype with more and larger stomatal clusters. Triple mutants cdka;1 flp-1 myb88 don't have guard cells stacks but accumulates sGCs (PubMed:24687979).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi14T → A: Increased kinase activity; when associated with F-15. 1 Publication1
Mutagenesisi15Y → F: Abolishes phosphorylation by WEE1. Increased kinase activity; when associated with A-14. 2 Publications1
Mutagenesisi146D → N: Decreased kinase activity and disturbed cell cycle. Reduced frequency of cell division during embryo development. 2 Publications1
Mutagenesisi156P → L: Decreased kinase activity and disturbed cell cycle. 1 Publication1
Mutagenesisi161T → D: Strong reduction in kinase activity and ability to bind substrate. Strong reduction in plant growth. Sterile plants. 1 Publication1
Mutagenesisi161T → V: Strong reduction in kinase activity and ability to bind substrate. 1 Publication1
Mutagenesisi166T → I: Decreased kinase activity and disturbed cell cycle. 1 Publication1
Mutagenesisi234 – 236Missing : No change in kinase activity, but disturbed cell cycle. Loss of interaction with CKS1. 1 Publication3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000857491 – 294Cyclin-dependent kinase A-1Add BLAST294

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15Phosphotyrosine1 Publication1
Modified residuei161PhosphothreonineCombined sources1 Publication1

Post-translational modificationi

Phosphorylated at Tyr-15 by WEE1. Phosphorylation at Thr-161 is important for the kinase activity and substrate binding. Binding to the anti-phosphatase PAS2 prevents dephosphorylation.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP24100.
PRIDEiP24100.

PTM databases

iPTMnetiP24100.

Expressioni

Tissue specificityi

Expressed in roots, stems, flowers and siliques.1 Publication

Developmental stagei

Expressed throughout the cell cycle. Expressed in actively dividing cells: root and shoot apical meristems, leaf primordia and emerging lateral root meristem. Expressed in light-grown seedlings from 1 up to 7 days after germination with a peak at 2 and 3 days.2 Publications

Inductioni

By nematode infection in roots. Down-regulated by salt stress in root meristem and replication blocking agents (hydroxyurea and aphidicolin).3 Publications

Gene expression databases

ExpressionAtlasiP24100. baseline and differential.
GenevisibleiP24100. AT.

Interactioni

Subunit structurei

Interacts with CDT1A, CYCA2-3, CYCD2-1, CYCD3-1, CYCD4-1, CYCD4-2, CYCH1-1, CYCU1-1, CYCU2-1, CYCU2-2, CYCU3-1, CYCU4-1, CYCU4-2, CYCU4-3, CKS1, KRP2/ICK2, KRP3/ICK6, KRP4/ICK7, KRP6/ICK4, KRP7/ICK5, and C-terminal domain of KRP1/ICK1. Interacts with WEE1 and TIF4A-1/EIF4A-1. Interacts with PAS2; when phosphorylated at Tyr-15. Interacts with SMR3, SMR4, SMR5, SMR6, SMR8 and At4g14310. Binds to CYCD3-2 (PubMed:24687979). Component of a DREAM-like complex which modulates a variety of developmentally regulated genes and of the mitotic genes in proliferating and differentiated cells. Interacts with MYB3R3 at later and with MYB3R4 at earlier stages of leaf development (PubMed:26069325).27 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi9354. 107 interactors.
IntActiP24100. 73 interactors.
STRINGi3702.AT3G48750.1.

Structurei

3D structure databases

ProteinModelPortaliP24100.
SMRiP24100.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 287Protein kinasePROSITE-ProRule annotationAdd BLAST284

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0594. Eukaryota.
ENOG410XPP3. LUCA.
HOGENOMiHOG000233024.
InParanoidiP24100.
KOiK02206.
OMAiEMMLVYD.
OrthoDBiEOG09360GJR.
PhylomeDBiP24100.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

P24100-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDQYEKVEKI GEGTYGVVYK ARDKVTNETI ALKKIRLEQE DEGVPSTAIR
60 70 80 90 100
EISLLKEMQH SNIVKLQDVV HSEKRLYLVF EYLDLDLKKH MDSTPDFSKD
110 120 130 140 150
LHMIKTYLYQ ILRGIAYCHS HRVLHRDLKP QNLLIDRRTN SLKLADFGLA
160 170 180 190 200
RAFGIPVRTF THEVVTLWYR APEILLGSHH YSTPVDIWSV GCIFAEMISQ
210 220 230 240 250
KPLFPGDSEI DQLFKIFRIM GTPYEDTWRG VTSLPDYKSA FPKWKPTDLE
260 270 280 290
TFVPNLDPDG VDLLSKMLLM DPTKRINARA ALEHEYFKDL GGMP
Length:294
Mass (Da):34,030
Last modified:March 1, 1992 - v1
Checksum:iB5FAE55FA9EC366E
GO

Sequence cautioni

The sequence AAL91258 differs from that shown. Reason: Frameshift at position 285.Curated
The sequence CAB87903 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59198 mRNA. Translation: AAA32831.1.
S45387 mRNA. Translation: AAB23643.1.
X57839 mRNA. Translation: CAA40971.1.
D10850 Genomic DNA. Translation: BAA01623.1.
AL132963 Genomic DNA. Translation: CAB87903.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE78452.1.
AY090353 mRNA. Translation: AAL91258.1. Frameshift.
BT024706 mRNA. Translation: ABD59044.1.
AK226373 mRNA. Translation: BAE98520.1.
AY085153 mRNA. Translation: AAM61706.1.
PIRiS23095.
T49271.
RefSeqiNP_566911.1. NM_114734.4.
UniGeneiAt.24166.

Genome annotation databases

EnsemblPlantsiAT3G48750.1; AT3G48750.1; AT3G48750.
GeneIDi824036.
GrameneiAT3G48750.1; AT3G48750.1; AT3G48750.
KEGGiath:AT3G48750.

Similar proteinsi

Entry informationi

Entry nameiCDKA1_ARATH
AccessioniPrimary (citable) accession number: P24100
Secondary accession number(s): Q29Q50, Q8RX68, Q9M307
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: September 27, 2017
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families