Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P24099 (GLNA1_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase cytosolic isozyme 1

EC=6.3.1.2
Alternative name(s):
GS1-1
Glutamate--ammonia ligase
OrganismGlycine max (Soybean) (Glycine hispida) [Reference proteome]
Taxonomic identifier3847 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja

Protein attributes

Sequence length355 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer.

Subcellular location

Cytoplasm.

Miscellaneous

Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Biological processNitrogen fixation
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

nitrogen fixation

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 355355Glutamine synthetase cytosolic isozyme 1
PRO_0000153196

Sequences

Sequence LengthMass (Da)Tools
P24099 [UniParc].

Last modified March 1, 1992. Version 1.
Checksum: 01A985684B1EF51D

FASTA35538,845
        10         20         30         40         50         60 
MSLLSDLINL NLSDTTEKVI AEYIWIGGSG MDLRSKARTL PGPVSDPSEL PKWNYDGSST 

        70         80         90        100        110        120 
GQAPGEDSEA IYTHKPFQDP FRRGNNILVI CDAYTPAGEP IPTNKRHAAA KVFSHPDVVA 

       130        140        150        160        170        180 
EVPWYGIEQE YTLLQKDIQW PLGWPVGGFP GPQGPYYCGV GADKAFGRDI VDAHYKACIY 

       190        200        210        220        230        240 
AGINISGING EVMPGQWEFQ VGPSVGISAG DEIWAARYIL ERITEIAGVV VSFDPKPIPG 

       250        260        270        280        290        300 
DWNGAGAHTN YSTKSMREDG GYEVIKAAID KLGKKHKEHI AAYGEGNERR LTGRHETADI 

       310        320        330        340        350 
NTFLWGVANR GASVRVGRDT EKAGKGYFED RRPASNMDPY VVTSMIADTT ILWKP 

« Hide

References

[1]"Ammonia-regulated expression of a soybean gene encoding cytosolic glutamine synthetase in transgenic Lotus corniculatus."
Miao G.-H., Hirel B., Marsolier M.C., Ridge R.W., Verma D.P.S.
Plant Cell 3:11-22(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Prize.
[2]"Forcing expression of a soybean root glutamine synthetase gene in tobacco leaves induces a native gene encoding cytosolic enzyme."
Hirel B., Marsolier M.C., Hoarau A., Hoarau J., Brangeon J., Schafer R., Verma D.P.S.
Plant Mol. Biol. 20:207-218(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 333-355.
Strain: cv. Prize.
Tissue: Root nodule.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S46513 mRNA. Translation: AAB23379.1.
X68598 mRNA. Translation: CAA48590.1.
PIRJQ0937.
RefSeqNP_001238531.1. NM_001251602.1.
UniGeneGma.32142.

3D structure databases

ProteinModelPortalP24099.
SMRP24099. Positions 4-354.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID548082.
KEGGgmx:548082.

Gene expression databases

GenevestigatorP24099.

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA1_SOYBN
AccessionPrimary (citable) accession number: P24099
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families