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Protein

Glutamine synthetase cytosolic isozyme 1

Gene
N/A
Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Nitrogen fixation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine synthetase cytosolic isozyme 1 (EC:6.3.1.2)
Alternative name(s):
GS1-1
Glutamate--ammonia ligase
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
ProteomesiUP000008827 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 355355Glutamine synthetase cytosolic isozyme 1PRO_0000153196Add
BLAST

Interactioni

Subunit structurei

Homooctamer.

Protein-protein interaction databases

STRINGi3847.GLYMA11G33560.1.

Structurei

3D structure databases

ProteinModelPortaliP24099.
SMRiP24099. Positions 4-354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamine synthetase family.Curated

Phylogenomic databases

KOiK01915.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24099-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLLSDLINL NLSDTTEKVI AEYIWIGGSG MDLRSKARTL PGPVSDPSEL
60 70 80 90 100
PKWNYDGSST GQAPGEDSEA IYTHKPFQDP FRRGNNILVI CDAYTPAGEP
110 120 130 140 150
IPTNKRHAAA KVFSHPDVVA EVPWYGIEQE YTLLQKDIQW PLGWPVGGFP
160 170 180 190 200
GPQGPYYCGV GADKAFGRDI VDAHYKACIY AGINISGING EVMPGQWEFQ
210 220 230 240 250
VGPSVGISAG DEIWAARYIL ERITEIAGVV VSFDPKPIPG DWNGAGAHTN
260 270 280 290 300
YSTKSMREDG GYEVIKAAID KLGKKHKEHI AAYGEGNERR LTGRHETADI
310 320 330 340 350
NTFLWGVANR GASVRVGRDT EKAGKGYFED RRPASNMDPY VVTSMIADTT

ILWKP
Length:355
Mass (Da):38,845
Last modified:March 1, 1992 - v1
Checksum:i01A985684B1EF51D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S46513 mRNA. Translation: AAB23379.1.
X68598 mRNA. Translation: CAA48590.1.
PIRiJQ0937.
RefSeqiNP_001238531.1. NM_001251602.1.
UniGeneiGma.32142.

Genome annotation databases

GeneIDi548082.
KEGGigmx:548082.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S46513 mRNA. Translation: AAB23379.1.
X68598 mRNA. Translation: CAA48590.1.
PIRiJQ0937.
RefSeqiNP_001238531.1. NM_001251602.1.
UniGeneiGma.32142.

3D structure databases

ProteinModelPortaliP24099.
SMRiP24099. Positions 4-354.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3847.GLYMA11G33560.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi548082.
KEGGigmx:548082.

Phylogenomic databases

KOiK01915.

Family and domain databases

Gene3Di3.30.590.10. 1 hit.
InterProiIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamiPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF54368. SSF54368. 1 hit.
PROSITEiPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Ammonia-regulated expression of a soybean gene encoding cytosolic glutamine synthetase in transgenic Lotus corniculatus."
    Miao G.-H., Hirel B., Marsolier M.C., Ridge R.W., Verma D.P.S.
    Plant Cell 3:11-22(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Prize.
  2. "Forcing expression of a soybean root glutamine synthetase gene in tobacco leaves induces a native gene encoding cytosolic enzyme."
    Hirel B., Marsolier M.C., Hoarau A., Hoarau J., Brangeon J., Schafer R., Verma D.P.S.
    Plant Mol. Biol. 20:207-218(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 333-355.
    Strain: cv. Prize.
    Tissue: Root nodule.

Entry informationi

Entry nameiGLNA1_SOYBN
AccessioniPrimary (citable) accession number: P24099
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: June 24, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.