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Protein

Seed linoleate 9S-lipoxygenase

Gene

LOX1.4

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Catalytic activityi

Linoleate + O2 = (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate.

Cofactori

Fe cationNote: Binds 1 Fe cation per subunit. Iron is tightly bound.

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi525Iron; catalytic1
Metal bindingi530Iron; catalytic1
Metal bindingi716Iron; catalytic1
Metal bindingi720Iron; catalytic1
Metal bindingi864Iron; via carboxylate; catalytic1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Seed linoleate 9S-lipoxygenase (EC:1.13.11.58)
Alternative name(s):
Lipoxygenase
Gene namesi
Name:LOX1.4
Synonyms:SC514
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
Proteomesi
  • UP000008827 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3120043.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002207211 – 864Seed linoleate 9S-lipoxygenaseAdd BLAST864

Proteomic databases

PRIDEiP24095.
ProMEXiP24095.

Expressioni

Tissue specificityi

Germinated cotyledons.

Inductioni

By jasmonate.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi3847.GLYMA07G00900.1.

Chemistry databases

BindingDBiP24095.

Structurei

Secondary structure

1864
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 19Combined sources9
Helixi20 – 22Combined sources3
Helixi25 – 28Combined sources4
Helixi58 – 60Combined sources3
Beta strandi66 – 76Combined sources11
Beta strandi78 – 80Combined sources3
Beta strandi82 – 89Combined sources8
Beta strandi92 – 94Combined sources3
Beta strandi105 – 111Combined sources7
Beta strandi120 – 127Combined sources8
Beta strandi129 – 131Combined sources3
Beta strandi133 – 141Combined sources9
Beta strandi144 – 146Combined sources3
Beta strandi149 – 157Combined sources9
Helixi160 – 162Combined sources3
Beta strandi163 – 165Combined sources3
Beta strandi167 – 172Combined sources6
Turni177 – 179Combined sources3
Helixi182 – 184Combined sources3
Helixi185 – 196Combined sources12
Helixi221 – 223Combined sources3
Beta strandi230 – 236Combined sources7
Helixi265 – 267Combined sources3
Helixi274 – 276Combined sources3
Helixi278 – 287Combined sources10
Helixi290 – 298Combined sources9
Helixi310 – 314Combined sources5
Turni315 – 317Combined sources3
Helixi325 – 331Combined sources7
Turni335 – 340Combined sources6
Beta strandi346 – 350Combined sources5
Beta strandi360 – 365Combined sources6
Helixi367 – 376Combined sources10
Beta strandi377 – 379Combined sources3
Beta strandi387 – 390Combined sources4
Helixi397 – 400Combined sources4
Helixi409 – 412Combined sources4
Helixi413 – 415Combined sources3
Turni416 – 418Combined sources3
Helixi421 – 426Combined sources6
Beta strandi430 – 434Combined sources5
Helixi436 – 439Combined sources4
Helixi440 – 442Combined sources3
Helixi443 – 447Combined sources5
Beta strandi456 – 463Combined sources8
Beta strandi469 – 478Combined sources10
Beta strandi481 – 486Combined sources6
Beta strandi489 – 493Combined sources5
Beta strandi497 – 500Combined sources4
Helixi501 – 522Combined sources22
Helixi523 – 529Combined sources7
Helixi530 – 543Combined sources14
Helixi549 – 554Combined sources6
Helixi555 – 558Combined sources4
Helixi561 – 571Combined sources11
Helixi578 – 582Combined sources5
Helixi586 – 588Combined sources3
Helixi589 – 597Combined sources9
Helixi602 – 604Combined sources3
Helixi607 – 613Combined sources7
Beta strandi616 – 619Combined sources4
Beta strandi621 – 623Combined sources3
Beta strandi626 – 632Combined sources7
Helixi635 – 658Combined sources24
Helixi662 – 667Combined sources6
Helixi669 – 680Combined sources12
Turni681 – 683Combined sources3
Helixi684 – 686Combined sources3
Beta strandi695 – 697Combined sources3
Helixi698 – 712Combined sources15
Helixi714 – 720Combined sources7
Helixi723 – 727Combined sources5
Turni730 – 732Combined sources3
Helixi747 – 754Combined sources8
Helixi756 – 761Combined sources6
Helixi767 – 780Combined sources14
Helixi802 – 825Combined sources24
Helixi832 – 834Combined sources3
Helixi836 – 838Combined sources3
Beta strandi851 – 853Combined sources3
Beta strandi859 – 861Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IUKX-ray2.40A/B1-864[»]
ProteinModelPortaliP24095.
SMRiP24095.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24095.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 171PLATPROSITE-ProRule annotationAdd BLAST128
Domaini174 – 864LipoxygenasePROSITE-ProRule annotationAdd BLAST691

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated
Contains 1 lipoxygenase domain.PROSITE-ProRule annotation
Contains 1 PLAT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IH0D. Eukaryota.
ENOG410YN4N. LUCA.
InParanoidiP24095.
KOiK15718.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 3 hits.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24095-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGIFDKGQK IKGTVVLMPK NVLDFNAITS IGKGGVIDTA TGILGQGVSL
60 70 80 90 100
VGGVIDTATS FLGRNISMQL ISATQTDGSG NGKVGKEVYL EKHLPTLPTL
110 120 130 140 150
GARQDAFSIF FEWDASFGIP GAFYIKNFMT DEFFLVSVKL EDIPNHGTIE
160 170 180 190 200
FVCNSWVYNF RSYKKNRIFF VNDTYLPSAT PAPLLKYRKE ELEVLRGDGT
210 220 230 240 250
GKRKDFDRIY DYDVYNDLGN PDGGDPRPIL GGSSIYPYPR RVRTGRERTR
260 270 280 290 300
TDPNSEKPGE VYVPRDENFG HLKSSDFLTY GIKSLSHDVI PLFKSAIFQL
310 320 330 340 350
RVTSSEFESF EDVRSLYEGG IKLPTDILSQ ISPLPALKEI FRTDGENVLQ
360 370 380 390 400
FPPPHVAKVS KSGWMTDEEF AREVIAGVNP NVIRRLQEFP PKSTLDPTLY
410 420 430 440 450
GDQTSTITKE QLEINMGGVT VEEALSTQRL FILDYQDAFI PYLTRINSLP
460 470 480 490 500
TAKAYATRTI LFLKDDGTLK PLAIELSKPH PDGDNLGPES IVVLPATEGV
510 520 530 540 550
DSTIWLLAKA HVIVNDSGYH QLVSHWLNTH AVMEPFAIAT NRHLSVLHPI
560 570 580 590 600
YKLLYPHYRD TININGLARQ SLINADGIIE KSFLPGKYSI EMSSSVYKNW
610 620 630 640 650
VFTDQALPAD LVKRGLAIED PSAPHGLRLV IEDYPYAVDG LEIWDAIKTW
660 670 680 690 700
VHEYVSLYYP TDAAVQQDTE LQAWWKEAVE KGHGDLKEKP WWPKMQTTED
710 720 730 740 750
LIQSCSIIVW TASALHAAVN FGQYPYGGLI LNRPTLARRF IPAEGTPEYD
760 770 780 790 800
EMVKNPQKAY LRTITPKFET LIDLSVIEIL SRHASDEIYL GERETPNWTT
810 820 830 840 850
DKKALEAFKR FGSKLTGIEG KINARNSDPS LRNRTGPVQL PYTLLHRSSE
860
EGLTFKGIPN SISI
Length:864
Mass (Da):96,817
Last modified:March 1, 1992 - v1
Checksum:i66F31FB1FA5F3B60
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti233S → C in AAA03728 (Ref. 2) Curated1
Sequence conflicti240R → L in AAA03728 (Ref. 2) Curated1
Sequence conflicti604D → H in AAA03728 (Ref. 2) Curated1
Sequence conflicti695M → K in AAA03728 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56139 Genomic DNA. Translation: CAA39604.1.
U04526 mRNA. Translation: AAA03728.1.
PIRiS13381.
RefSeqiNP_001238676.1. NM_001251747.1.
UniGeneiGma.2019.

Genome annotation databases

GeneIDi547835.
KEGGigmx:547835.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56139 Genomic DNA. Translation: CAA39604.1.
U04526 mRNA. Translation: AAA03728.1.
PIRiS13381.
RefSeqiNP_001238676.1. NM_001251747.1.
UniGeneiGma.2019.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2IUKX-ray2.40A/B1-864[»]
ProteinModelPortaliP24095.
SMRiP24095.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3847.GLYMA07G00900.1.

Chemistry databases

BindingDBiP24095.
ChEMBLiCHEMBL3120043.

Proteomic databases

PRIDEiP24095.
ProMEXiP24095.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi547835.
KEGGigmx:547835.

Phylogenomic databases

eggNOGiENOG410IH0D. Eukaryota.
ENOG410YN4N. LUCA.
InParanoidiP24095.
KOiK15718.

Enzyme and pathway databases

UniPathwayiUPA00382.

Miscellaneous databases

EvolutionaryTraceiP24095.
PROiP24095.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 3 hits.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLOXX_SOYBN
AccessioniPrimary (citable) accession number: P24095
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: November 2, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Soybean contains at least 4 distinct isoenzymes, L-1, L-2, L-3a and L-3b in dry seeds, and at least two distinct isozymes in the hypocotyl/radicle region of the seedling stem.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.