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Protein

Seed linoleate 9S-lipoxygenase

Gene

LOX1.4

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Catalytic activityi

Linoleate + O2 = (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate.

Cofactori

Fe cationNote: Binds 1 Fe cation per subunit. Iron is tightly bound.

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi525 – 5251Iron; catalytic
Metal bindingi530 – 5301Iron; catalytic
Metal bindingi716 – 7161Iron; catalytic
Metal bindingi720 – 7201Iron; catalytic
Metal bindingi864 – 8641Iron; via carboxylate; catalytic

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Seed linoleate 9S-lipoxygenase (EC:1.13.11.58)
Alternative name(s):
Lipoxygenase
Gene namesi
Name:LOX1.4
Synonyms:SC514
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
Proteomesi
  • UP000008827 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3120043.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 864864Seed linoleate 9S-lipoxygenasePRO_0000220721Add
BLAST

Proteomic databases

ProMEXiP24095.

Expressioni

Tissue specificityi

Germinated cotyledons.

Inductioni

By jasmonate.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi3847.GLYMA07G00900.1.

Chemistry

BindingDBiP24095.

Structurei

Secondary structure

1
864
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 199Combined sources
Helixi20 – 223Combined sources
Helixi25 – 284Combined sources
Helixi58 – 603Combined sources
Beta strandi66 – 7611Combined sources
Beta strandi78 – 803Combined sources
Beta strandi82 – 898Combined sources
Beta strandi92 – 943Combined sources
Beta strandi105 – 1117Combined sources
Beta strandi120 – 1278Combined sources
Beta strandi129 – 1313Combined sources
Beta strandi133 – 1419Combined sources
Beta strandi144 – 1463Combined sources
Beta strandi149 – 1579Combined sources
Helixi160 – 1623Combined sources
Beta strandi163 – 1653Combined sources
Beta strandi167 – 1726Combined sources
Turni177 – 1793Combined sources
Helixi182 – 1843Combined sources
Helixi185 – 19612Combined sources
Helixi221 – 2233Combined sources
Beta strandi230 – 2367Combined sources
Helixi265 – 2673Combined sources
Helixi274 – 2763Combined sources
Helixi278 – 28710Combined sources
Helixi290 – 2989Combined sources
Helixi310 – 3145Combined sources
Turni315 – 3173Combined sources
Helixi325 – 3317Combined sources
Turni335 – 3406Combined sources
Beta strandi346 – 3505Combined sources
Beta strandi360 – 3656Combined sources
Helixi367 – 37610Combined sources
Beta strandi377 – 3793Combined sources
Beta strandi387 – 3904Combined sources
Helixi397 – 4004Combined sources
Helixi409 – 4124Combined sources
Helixi413 – 4153Combined sources
Turni416 – 4183Combined sources
Helixi421 – 4266Combined sources
Beta strandi430 – 4345Combined sources
Helixi436 – 4394Combined sources
Helixi440 – 4423Combined sources
Helixi443 – 4475Combined sources
Beta strandi456 – 4638Combined sources
Beta strandi469 – 47810Combined sources
Beta strandi481 – 4866Combined sources
Beta strandi489 – 4935Combined sources
Beta strandi497 – 5004Combined sources
Helixi501 – 52222Combined sources
Helixi523 – 5297Combined sources
Helixi530 – 54314Combined sources
Helixi549 – 5546Combined sources
Helixi555 – 5584Combined sources
Helixi561 – 57111Combined sources
Helixi578 – 5825Combined sources
Helixi586 – 5883Combined sources
Helixi589 – 5979Combined sources
Helixi602 – 6043Combined sources
Helixi607 – 6137Combined sources
Beta strandi616 – 6194Combined sources
Beta strandi621 – 6233Combined sources
Beta strandi626 – 6327Combined sources
Helixi635 – 65824Combined sources
Helixi662 – 6676Combined sources
Helixi669 – 68012Combined sources
Turni681 – 6833Combined sources
Helixi684 – 6863Combined sources
Beta strandi695 – 6973Combined sources
Helixi698 – 71215Combined sources
Helixi714 – 7207Combined sources
Helixi723 – 7275Combined sources
Turni730 – 7323Combined sources
Helixi747 – 7548Combined sources
Helixi756 – 7616Combined sources
Helixi767 – 78014Combined sources
Helixi802 – 82524Combined sources
Helixi832 – 8343Combined sources
Helixi836 – 8383Combined sources
Beta strandi851 – 8533Combined sources
Beta strandi859 – 8613Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IUKX-ray2.40A/B1-864[»]
ProteinModelPortaliP24095.
SMRiP24095. Positions 8-864.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP24095.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini44 – 171128PLATPROSITE-ProRule annotationAdd
BLAST
Domaini174 – 864691LipoxygenasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated
Contains 1 lipoxygenase domain.PROSITE-ProRule annotation
Contains 1 PLAT domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IH0D. Eukaryota.
ENOG410YN4N. LUCA.
InParanoidiP24095.
KOiK15718.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 3 hits.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P24095-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFGIFDKGQK IKGTVVLMPK NVLDFNAITS IGKGGVIDTA TGILGQGVSL
60 70 80 90 100
VGGVIDTATS FLGRNISMQL ISATQTDGSG NGKVGKEVYL EKHLPTLPTL
110 120 130 140 150
GARQDAFSIF FEWDASFGIP GAFYIKNFMT DEFFLVSVKL EDIPNHGTIE
160 170 180 190 200
FVCNSWVYNF RSYKKNRIFF VNDTYLPSAT PAPLLKYRKE ELEVLRGDGT
210 220 230 240 250
GKRKDFDRIY DYDVYNDLGN PDGGDPRPIL GGSSIYPYPR RVRTGRERTR
260 270 280 290 300
TDPNSEKPGE VYVPRDENFG HLKSSDFLTY GIKSLSHDVI PLFKSAIFQL
310 320 330 340 350
RVTSSEFESF EDVRSLYEGG IKLPTDILSQ ISPLPALKEI FRTDGENVLQ
360 370 380 390 400
FPPPHVAKVS KSGWMTDEEF AREVIAGVNP NVIRRLQEFP PKSTLDPTLY
410 420 430 440 450
GDQTSTITKE QLEINMGGVT VEEALSTQRL FILDYQDAFI PYLTRINSLP
460 470 480 490 500
TAKAYATRTI LFLKDDGTLK PLAIELSKPH PDGDNLGPES IVVLPATEGV
510 520 530 540 550
DSTIWLLAKA HVIVNDSGYH QLVSHWLNTH AVMEPFAIAT NRHLSVLHPI
560 570 580 590 600
YKLLYPHYRD TININGLARQ SLINADGIIE KSFLPGKYSI EMSSSVYKNW
610 620 630 640 650
VFTDQALPAD LVKRGLAIED PSAPHGLRLV IEDYPYAVDG LEIWDAIKTW
660 670 680 690 700
VHEYVSLYYP TDAAVQQDTE LQAWWKEAVE KGHGDLKEKP WWPKMQTTED
710 720 730 740 750
LIQSCSIIVW TASALHAAVN FGQYPYGGLI LNRPTLARRF IPAEGTPEYD
760 770 780 790 800
EMVKNPQKAY LRTITPKFET LIDLSVIEIL SRHASDEIYL GERETPNWTT
810 820 830 840 850
DKKALEAFKR FGSKLTGIEG KINARNSDPS LRNRTGPVQL PYTLLHRSSE
860
EGLTFKGIPN SISI
Length:864
Mass (Da):96,817
Last modified:March 1, 1992 - v1
Checksum:i66F31FB1FA5F3B60
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti233 – 2331S → C in AAA03728 (Ref. 2) Curated
Sequence conflicti240 – 2401R → L in AAA03728 (Ref. 2) Curated
Sequence conflicti604 – 6041D → H in AAA03728 (Ref. 2) Curated
Sequence conflicti695 – 6951M → K in AAA03728 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56139 Genomic DNA. Translation: CAA39604.1.
U04526 mRNA. Translation: AAA03728.1.
PIRiS13381.
RefSeqiNP_001238676.1. NM_001251747.1.
UniGeneiGma.2019.

Genome annotation databases

GeneIDi547835.
KEGGigmx:547835.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56139 Genomic DNA. Translation: CAA39604.1.
U04526 mRNA. Translation: AAA03728.1.
PIRiS13381.
RefSeqiNP_001238676.1. NM_001251747.1.
UniGeneiGma.2019.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IUKX-ray2.40A/B1-864[»]
ProteinModelPortaliP24095.
SMRiP24095. Positions 8-864.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3847.GLYMA07G00900.1.

Chemistry

BindingDBiP24095.
ChEMBLiCHEMBL3120043.

Proteomic databases

ProMEXiP24095.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi547835.
KEGGigmx:547835.

Phylogenomic databases

eggNOGiENOG410IH0D. Eukaryota.
ENOG410YN4N. LUCA.
InParanoidiP24095.
KOiK15718.

Enzyme and pathway databases

UniPathwayiUPA00382.

Miscellaneous databases

EvolutionaryTraceiP24095.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_plant.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 3 hits.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequences of a soybean lipoxygenase gene and the short intergenic region between an upstream lipoxygenase gene."
    Shibata D., Kato T., Tanaka K.
    Plant Mol. Biol. 16:353-359(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Cotyledon.
  2. Park T., Holland M.A., Laskey J.G., Polacco J.C.
    Submitted (DEC-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Williams 82.
    Tissue: Radicle.
  3. "Crystal structures of vegetative soybean lipoxygenase VLX-B and VLX-D, and comparisons with seed isoforms LOX-1 and LOX-3."
    Youn B., Sellhorn G.E., Mirchel R.J., Gaffney B.J., Grimes H.D., Kang C.
    Proteins 65:1008-1020(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH IRON IONS.

Entry informationi

Entry nameiLOXX_SOYBN
AccessioniPrimary (citable) accession number: P24095
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 1992
Last sequence update: March 1, 1992
Last modified: December 9, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Soybean contains at least 4 distinct isoenzymes, L-1, L-2, L-3a and L-3b in dry seeds, and at least two distinct isozymes in the hypocotyl/radicle region of the seedling stem.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.